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The map label for this gene is napB [H]

Identifier: 238919149

GI number: 238919149

Start: 1217001

End: 1217453

Strand: Direct

Name: napB [H]

Synonym: NT01EI_1220

Alternate gene names: 238919149

Gene position: 1217001-1217453 (Clockwise)

Preceding gene: 238919148

Following gene: 238919150

Centisome position: 31.92

GC content: 60.71

Gene sequence:

>453_bases
ATGAAGAGCAATGTCCTGAAGAAGCGGTTCTCCCTGGGGGCACTGTTATTATCATTGGCGTTGTGCGGCGCGGCTTCGGC
CGCCGACGATGCCGTTAATCTGGCGCAGTCACCGGAGGTGTCCGGTACGGCCGAAGGGCAGATCAGGATGCCAAAGCAGC
AGGAGCGCATGGCACTGAACTATGTTAACCAGCCGCCGATGATCCCGCACAGCGTGGACGGCTACCAGATCACCAAAAAT
ACCAACCGCTGCCTGCAGTGCCATGGGGTGGAGAATTACCGCACCACCGGGGCGATGCGCGTCAGCCCGACCCATTTCAT
CGACGCCAGCGGCAAGATGCTGGGACAGGTGGCGCCCAACCGCTACTTCTGCCTGCAGTGCCACGTACCGCAGACCGATG
CACCGCCGATCGTCGGGAATACCTTTACCCCGTCCAGCGGCTTTGGTCAGTAA

Upstream 100 bases:

>100_bases
TATTGAGCAAGGCGTGCATCAGCTGCGGGCGCTGCATGGACGTATGTGCTGAGCAGGTATTTGAATTTAAGACACGATTT
CATCGTTCGGGAGATAAACA

Downstream 100 bases:

>100_bases
AAGGGGCCTATGATGAGCGAGCAACATAACGCCCCGACGGGCAACAAGCCGGGCCTGCTGCGCCGGGTGTGGCAGTGGTG
GCGTCGTCCCAGTCGTCTGG

Product: citrate reductase cytochrome c-type subunit

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MKSNVLKKRFSLGALLLSLALCGAASAADDAVNLAQSPEVSGTAEGQIRMPKQQERMALNYVNQPPMIPHSVDGYQITKN
TNRCLQCHGVENYRTTGAMRVSPTHFIDASGKMLGQVAPNRYFCLQCHVPQTDAPPIVGNTFTPSSGFGQ

Sequences:

>Translated_150_residues
MKSNVLKKRFSLGALLLSLALCGAASAADDAVNLAQSPEVSGTAEGQIRMPKQQERMALNYVNQPPMIPHSVDGYQITKN
TNRCLQCHGVENYRTTGAMRVSPTHFIDASGKMLGQVAPNRYFCLQCHVPQTDAPPIVGNTFTPSSGFGQ
>Mature_150_residues
MKSNVLKKRFSLGALLLSLALCGAASAADDAVNLAQSPEVSGTAEGQIRMPKQQERMALNYVNQPPMIPHSVDGYQITKN
TNRCLQCHGVENYRTTGAMRVSPTHFIDASGKMLGQVAPNRYFCLQCHVPQTDAPPIVGNTFTPSSGFGQ

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=150, Percent_Identity=68.6666666666667, Blast_Score=223, Evalue=5e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 16180; Mature: 16180

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSNVLKKRFSLGALLLSLALCGAASAADDAVNLAQSPEVSGTAEGQIRMPKQQERMALN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEECCHHHHHHHHH
YVNQPPMIPHSVDGYQITKNTNRCLQCHGVENYRTTGAMRVSPTHFIDASGKMLGQVAPN
CCCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEECCCEEECCCCCEEEECCCC
RYFCLQCHVPQTDAPPIVGNTFTPSSGFGQ
EEEEEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSNVLKKRFSLGALLLSLALCGAASAADDAVNLAQSPEVSGTAEGQIRMPKQQERMALN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEECCHHHHHHHHH
YVNQPPMIPHSVDGYQITKNTNRCLQCHGVENYRTTGAMRVSPTHFIDASGKMLGQVAPN
CCCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEECCCEEECCCCCEEEECCCC
RYFCLQCHVPQTDAPPIVGNTFTPSSGFGQ
EEEEEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 8039676 [H]