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The map label for this gene is napA

Identifier: 238919146

GI number: 238919146

Start: 1212885

End: 1215374

Strand: Direct

Name: napA

Synonym: NT01EI_1217

Alternate gene names: 238919146

Gene position: 1212885-1215374 (Clockwise)

Preceding gene: 238919145

Following gene: 238919147

Centisome position: 31.81

GC content: 60.76

Gene sequence:

>2490_bases
ATGAAACTCAGTCGTCGAGACTTCATGAAAGCTAACGCTGTTGCTGCCGCTGCCGCTGCAGCGGGGCTGACTATCCCGAC
CGTGGCACGCGCGGTGACGGATGCCGGATCGGATGGTATCACGTGGGATAAGGCGCCGTGCCGCTTTTGCGGTACCGGCT
GCGGGGTGCTTGTCGGCACCCAGAATGGCCGCATCGTGGCCTCTCAGGGGGACCCGGATGCGCCGGTCAACCGTGGGCTG
AACTGCATTAAAGGCTATTTCCTGCCCAAGATCATGTACGGTAAGGATCGTCTGACCCAGCCGCTGCTGCGGATGAAGGG
AGGCCAGTATGACAAAGAGGGCGAGTTTACGCCGGTCAGCTGGGATCAGGCGTTTGATGTGATGGCCGCCAAGTTTAAGG
GGACGCTGAAAGAGAAGGGGCCGGGTGCCGTCGGGATGTTTGGCTCCGGACAGTGGACGGTCTGGGAGGGGTACGCCGCT
GCCAAGCTGTTCAAGGCCGGCTTCCGCTCCAATAACCTCGACCCCAACGCCCGCCACTGCATGGCCTCCGCGGTGGTCGG
CTTTATGCGTACCTTCGGTATGGATGAGCCGATGGGCTGCTATGATGACATCGAGCAGGCCGATGCCTTTGTGCTGTGGG
GCTCCAACATGGCGGAGATGCACCCGATCCTGTGGTCGCGTATCACCAACCGCCGTCTGAGCAACGATAAGGTGCAGGTC
GCGGTGTTGTCCACCTTCGAGCAGCGTAGCTTTGAGCTGGCGGACAACCCGATTATCTTCACCCCGCAGTCCGATCTGGT
CATCCTGAACTACATCGCTAACTACATTATCCAGAATAACGCCGTTGATCAGGCATTTATGGATAAGCATGTCAATATTC
GCAAAGGGGCGACCGATATCGGCTACGGCCTGCGTCCGACCGATCCGCTGGAGAAGGCGGCGAAGAACCCGGGCAGCGAT
GCCTCTGAGCCGATGAGCTTCGACGACTATAAAGCCTTTGTGGCCGATTATACCCTGGAAAAAACCGCCAAGATGACCGG
TGTGCCGCAGGATCAGCTGCTAGCGCTGGCCAAGCTGTATGCCGACCCAAATATCAAGGTAGTCTCTTACTGGACCATGG
GCTTTAACCAGCATACCCGTGGGGTATGGGCCAATAACCTGTGCTACAACATTCACCTGCTGACCGGCAAGATCTCCAAG
CCGGGCTGTGGGCCATTCTCCCTGACCGGCCAGCCGTCGGCCTGTGGCACCGCGCGTGAGGTCGGTACCTTTGCCCACCG
TCTACCGGCGGATATGGTGGTAACCAACGAAAAACACCGTCAGATCGCCGAAACCCGCTGGAAGATCCCGGCCGGAACCA
TTCCGGACAAGGTGGGGCTGCATGCCGTCGCCCAGGACCGCGCCCTGAAAGACGGCAAGCTCAATGCCTATTGGGTGATG
TGTAACAACAACATGCAGGCTGGTCCGAACATCAATGCGGATCGTATGCCGGGCTGGCGTGATCCGCGTAACTTTATTGT
GGTCTCCGATCCCTACCCGACGGTGAGCGCACTCTCCGCCGACCTGATCCTGCCGACCGCCATGTGGGTGGAGAAAGAGG
GGGCCTACGGCAACGCCGAGCGCCGCACCCAGTTCTGGCACCAGCAGGTGAAGGCGCCGGGGGAGGCCAAGTCGGATCTG
TGGCAGCTGATGGAGTTCTCCAAGCGTTTCACCGTCGATGAGGTCTGGCCGGCGGCGCTGCTGGCGAAGATGCCGGCGCT
GCAGGGAAAAACGCTGTATGAGGTGCTGTATGTCAACGGTAACGTCGACAGGTATCCGCTGGATGAGGTGCCGGCCGATC
GCCTGAACGATGAGGCGCGCGCCTGTGGCTTCTACGTGCAGAAAGGGCTGTTTGAGGAGTACGCCGGTTTTGGCCGCGGC
CACGGCCACGATCTGGCGCCGTTCGATGCCTATCACAAGGCACGCGGTATCCGCTGGCCGGTGGTTGACGGCAAAGAGAC
GCTGTGGCGCTACCGCGAAGGCTACGATCCGTTCGTCAAGAACGGGGAAGAGGTGCGCTTCTACGGCAAGCCGGATGGTA
AGGCGGTGATCTTTGCGCTGCCCTATGAGCCGCCCGCCGAGAGTCCGGATCAGGAGTACGACCTGTGGCTGTCTACCGGC
CGCGTTCTGGAGCACTGGCATACCGGTTCGATGACCCGACGGGTCCCGGAGCTGCACCGTGCGTTCCCTGAGGCGGTGCT
GTTTATCCATCCGCTGGATGCCAAGGCGCGCGGCCTGCGCCGCGGCGACAAGGTTAAGGTGATGTCGCGCCGCGGCGAGC
TGGTGTCCATTGTCGAGACCCGCGGCCGTAACCGCGTACCGCAGGGGTTGGTCTACATGCCGTTCTTCGATGCCGCCCAG
CTGGTTAATGTGCTGACACTGGACGCCACCGATCCGCTGTCCAAAGAGGCCGATTTCAAGAAGTGCGCAGTGAAGATTGA
GAAGGTGTAA

Upstream 100 bases:

>100_bases
GCAGGCTGCTGGATCGAATTGAGTCGGTGCGCAATGTAGCTGGCGTGCTGGCGGTGTCGCTGGTTTATCACCAGCAGGAT
GAGCAAGGTGAGGATACGCC

Downstream 100 bases:

>100_bases
GGACGGCGCAGATGACGGAACAACGGGATAAGGGACCGGCACGGCGGCGCTTCTTGCGCGATGCCGTGCGCGGTGCGGGT
GGACTGGCGGCCGTGGCCCT

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 829; Mature: 829

Protein sequence:

>829_residues
MKLSRRDFMKANAVAAAAAAAGLTIPTVARAVTDAGSDGITWDKAPCRFCGTGCGVLVGTQNGRIVASQGDPDAPVNRGL
NCIKGYFLPKIMYGKDRLTQPLLRMKGGQYDKEGEFTPVSWDQAFDVMAAKFKGTLKEKGPGAVGMFGSGQWTVWEGYAA
AKLFKAGFRSNNLDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAEMHPILWSRITNRRLSNDKVQV
AVLSTFEQRSFELADNPIIFTPQSDLVILNYIANYIIQNNAVDQAFMDKHVNIRKGATDIGYGLRPTDPLEKAAKNPGSD
ASEPMSFDDYKAFVADYTLEKTAKMTGVPQDQLLALAKLYADPNIKVVSYWTMGFNQHTRGVWANNLCYNIHLLTGKISK
PGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRQIAETRWKIPAGTIPDKVGLHAVAQDRALKDGKLNAYWVM
CNNNMQAGPNINADRMPGWRDPRNFIVVSDPYPTVSALSADLILPTAMWVEKEGAYGNAERRTQFWHQQVKAPGEAKSDL
WQLMEFSKRFTVDEVWPAALLAKMPALQGKTLYEVLYVNGNVDRYPLDEVPADRLNDEARACGFYVQKGLFEEYAGFGRG
HGHDLAPFDAYHKARGIRWPVVDGKETLWRYREGYDPFVKNGEEVRFYGKPDGKAVIFALPYEPPAESPDQEYDLWLSTG
RVLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARGLRRGDKVKVMSRRGELVSIVETRGRNRVPQGLVYMPFFDAAQ
LVNVLTLDATDPLSKEADFKKCAVKIEKV

Sequences:

>Translated_829_residues
MKLSRRDFMKANAVAAAAAAAGLTIPTVARAVTDAGSDGITWDKAPCRFCGTGCGVLVGTQNGRIVASQGDPDAPVNRGL
NCIKGYFLPKIMYGKDRLTQPLLRMKGGQYDKEGEFTPVSWDQAFDVMAAKFKGTLKEKGPGAVGMFGSGQWTVWEGYAA
AKLFKAGFRSNNLDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAEMHPILWSRITNRRLSNDKVQV
AVLSTFEQRSFELADNPIIFTPQSDLVILNYIANYIIQNNAVDQAFMDKHVNIRKGATDIGYGLRPTDPLEKAAKNPGSD
ASEPMSFDDYKAFVADYTLEKTAKMTGVPQDQLLALAKLYADPNIKVVSYWTMGFNQHTRGVWANNLCYNIHLLTGKISK
PGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRQIAETRWKIPAGTIPDKVGLHAVAQDRALKDGKLNAYWVM
CNNNMQAGPNINADRMPGWRDPRNFIVVSDPYPTVSALSADLILPTAMWVEKEGAYGNAERRTQFWHQQVKAPGEAKSDL
WQLMEFSKRFTVDEVWPAALLAKMPALQGKTLYEVLYVNGNVDRYPLDEVPADRLNDEARACGFYVQKGLFEEYAGFGRG
HGHDLAPFDAYHKARGIRWPVVDGKETLWRYREGYDPFVKNGEEVRFYGKPDGKAVIFALPYEPPAESPDQEYDLWLSTG
RVLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARGLRRGDKVKVMSRRGELVSIVETRGRNRVPQGLVYMPFFDAAQ
LVNVLTLDATDPLSKEADFKKCAVKIEKV
>Mature_829_residues
MKLSRRDFMKANAVAAAAAAAGLTIPTVARAVTDAGSDGITWDKAPCRFCGTGCGVLVGTQNGRIVASQGDPDAPVNRGL
NCIKGYFLPKIMYGKDRLTQPLLRMKGGQYDKEGEFTPVSWDQAFDVMAAKFKGTLKEKGPGAVGMFGSGQWTVWEGYAA
AKLFKAGFRSNNLDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAEMHPILWSRITNRRLSNDKVQV
AVLSTFEQRSFELADNPIIFTPQSDLVILNYIANYIIQNNAVDQAFMDKHVNIRKGATDIGYGLRPTDPLEKAAKNPGSD
ASEPMSFDDYKAFVADYTLEKTAKMTGVPQDQLLALAKLYADPNIKVVSYWTMGFNQHTRGVWANNLCYNIHLLTGKISK
PGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRQIAETRWKIPAGTIPDKVGLHAVAQDRALKDGKLNAYWVM
CNNNMQAGPNINADRMPGWRDPRNFIVVSDPYPTVSALSADLILPTAMWVEKEGAYGNAERRTQFWHQQVKAPGEAKSDL
WQLMEFSKRFTVDEVWPAALLAKMPALQGKTLYEVLYVNGNVDRYPLDEVPADRLNDEARACGFYVQKGLFEEYAGFGRG
HGHDLAPFDAYHKARGIRWPVVDGKETLWRYREGYDPFVKNGEEVRFYGKPDGKAVIFALPYEPPAESPDQEYDLWLSTG
RVLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARGLRRGDKVKVMSRRGELVSIVETRGRNRVPQGLVYMPFFDAAQ
LVNVLTLDATDPLSKEADFKKCAVKIEKV

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily

Homologues:

Organism=Escherichia coli, GI1788534, Length=829, Percent_Identity=84.9215922798552, Blast_Score=1478, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=794, Percent_Identity=24.0554156171285, Blast_Score=208, Evalue=1e-54,
Organism=Escherichia coli, GI3868720, Length=664, Percent_Identity=23.9457831325301, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI87081797, Length=901, Percent_Identity=23.5294117647059, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI3868719, Length=664, Percent_Identity=22.5903614457831, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1787870, Length=891, Percent_Identity=23.1200897867565, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI171474008, Length=888, Percent_Identity=22.5225225225225, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1787778, Length=406, Percent_Identity=24.1379310344828, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPA_EDWI9 (C5B7B9)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002932660.1
- ProteinModelPortal:   C5B7B9
- GeneID:   7960963
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1217
- OMA:   IISAPYE
- ProtClustDB:   PRK13532
- GO:   GO:0006810
- HAMAP:   MF_01630
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311
- Gene3D:   G3DSA:2.40.40.20
- SMART:   SM00926
- TIGRFAMs:   TIGR01706
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; SSF50692 Asp_decarb_fold

EC number: =1.7.99.4

Molecular weight: Translated: 92242; Mature: 92242

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1; PS00490 MOLYBDOPTERIN_PROK_2; PS00932 MOLYBDOPTERIN_PROK_3; PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSRRDFMKANAVAAAAAAAGLTIPTVARAVTDAGSDGITWDKAPCRFCGTGCGVLVGT
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEECCCCCHHHCCCCEEEEEC
QNGRIVASQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLLRMKGGQYDKEGEFTPVS
CCCEEEECCCCCCCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
WDQAFDVMAAKFKGTLKEKGPGAVGMFGSGQWTVWEGYAAAKLFKAGFRSNNLDPNARHC
HHHHHHHHHHHHHCHHHHCCCCEEEECCCCCEEEECCHHHHHHHHHHCCCCCCCCHHHHH
MASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAEMHPILWSRITNRRLSNDKVQV
HHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEE
AVLSTFEQRSFELADNPIIFTPQSDLVILNYIANYIIQNNAVDQAFMDKHVNIRKGATDI
EEEEHHHHHCCEECCCCEEECCCCCEEHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCC
GYGLRPTDPLEKAAKNPGSDASEPMSFDDYKAFVADYTLEKTAKMTGVPQDQLLALAKLY
CCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ADPNIKVVSYWTMGFNQHTRGVWANNLCYNIHLLTGKISKPGCGPFSLTGQPSACGTARE
CCCCEEEEEEEEECCCCCCCCEEECCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHH
VGTFAHRLPADMVVTNEKHRQIAETRWKIPAGTIPDKVGLHAVAQDRALKDGKLNAYWVM
HHHHHHHCCCCEEEECHHHHHHHHHHCCCCCCCCCCHHCHHHHHHCCCCCCCCCEEEEEE
CNNNMQAGPNINADRMPGWRDPRNFIVVSDPYPTVSALSADLILPTAMWVEKEGAYGNAE
ECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHEEECCCCCCCHH
RRTQFWHQQVKAPGEAKSDLWQLMEFSKRFTVDEVWPAALLAKMPALQGKTLYEVLYVNG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECC
NVDRYPLDEVPADRLNDEARACGFYVQKGLFEEYAGFGRGHGHDLAPFDAYHKARGIRWP
CCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCC
VVDGKETLWRYREGYDPFVKNGEEVRFYGKPDGKAVIFALPYEPPAESPDQEYDLWLSTG
CCCCHHHHHHHHCCCCHHHCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCHHEEECCC
RVLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARGLRRGDKVKVMSRRGELVSIVET
HHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCCCCEEEEHHCCCEEEEHHH
RGRNRVPQGLVYMPFFDAAQLVNVLTLDATDPLSKEADFKKCAVKIEKV
CCCCCCCCCEEECCCCCHHHHHHEEEECCCCCCCCCCCHHHHHEEEECC
>Mature Secondary Structure
MKLSRRDFMKANAVAAAAAAAGLTIPTVARAVTDAGSDGITWDKAPCRFCGTGCGVLVGT
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEECCCCCHHHCCCCEEEEEC
QNGRIVASQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLLRMKGGQYDKEGEFTPVS
CCCEEEECCCCCCCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
WDQAFDVMAAKFKGTLKEKGPGAVGMFGSGQWTVWEGYAAAKLFKAGFRSNNLDPNARHC
HHHHHHHHHHHHHCHHHHCCCCEEEECCCCCEEEECCHHHHHHHHHHCCCCCCCCHHHHH
MASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAEMHPILWSRITNRRLSNDKVQV
HHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEE
AVLSTFEQRSFELADNPIIFTPQSDLVILNYIANYIIQNNAVDQAFMDKHVNIRKGATDI
EEEEHHHHHCCEECCCCEEECCCCCEEHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCC
GYGLRPTDPLEKAAKNPGSDASEPMSFDDYKAFVADYTLEKTAKMTGVPQDQLLALAKLY
CCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ADPNIKVVSYWTMGFNQHTRGVWANNLCYNIHLLTGKISKPGCGPFSLTGQPSACGTARE
CCCCEEEEEEEEECCCCCCCCEEECCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHH
VGTFAHRLPADMVVTNEKHRQIAETRWKIPAGTIPDKVGLHAVAQDRALKDGKLNAYWVM
HHHHHHHCCCCEEEECHHHHHHHHHHCCCCCCCCCCHHCHHHHHHCCCCCCCCCEEEEEE
CNNNMQAGPNINADRMPGWRDPRNFIVVSDPYPTVSALSADLILPTAMWVEKEGAYGNAE
ECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHEEECCCCCCCHH
RRTQFWHQQVKAPGEAKSDLWQLMEFSKRFTVDEVWPAALLAKMPALQGKTLYEVLYVNG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECC
NVDRYPLDEVPADRLNDEARACGFYVQKGLFEEYAGFGRGHGHDLAPFDAYHKARGIRWP
CCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCC
VVDGKETLWRYREGYDPFVKNGEEVRFYGKPDGKAVIFALPYEPPAESPDQEYDLWLSTG
CCCCHHHHHHHHCCCCHHHCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCHHEEECCC
RVLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARGLRRGDKVKVMSRRGELVSIVET
HHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCCCCEEEEHHCCCEEEEHHH
RGRNRVPQGLVYMPFFDAAQLVNVLTLDATDPLSKEADFKKCAVKIEKV
CCCCCCCCCEEECCCCCHHHHHHEEEECCCCCCCCCCCHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA