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The map label for this gene is thiM [H]

Identifier: 238919082

GI number: 238919082

Start: 1147161

End: 1147964

Strand: Direct

Name: thiM [H]

Synonym: NT01EI_1152

Alternate gene names: 238919082

Gene position: 1147161-1147964 (Clockwise)

Preceding gene: 238919081

Following gene: 238919083

Centisome position: 30.09

GC content: 68.03

Gene sequence:

>804_bases
ATGTTGACACAACCCACCCCCCTGCCTGATGCGCTGTGCGCCCAGGCGCTGATGACCTTACGCCAGCGCGCGCCGCTGGT
TCACTGCCTGACCAATGAAGTGGTGCAAAGCCTGACGGCCAACGTGCTGTTGGCGCTGGGCGCCGCTCCGGCGATGGTTG
TGCAGGCTGAGGAGGCCCGCCAGTTTGCAGCGCTGGCCGATGCGCTGCTGATTAATATCGGTACGTTGTATGACACGCGC
GCCAACAGTATGCTGGCGGCGATTGAGTCGGCCAACCTGGCAGGAACCCCCTGGGTACTGGATCCGGTGGCCGTCGGGGC
GCTGCGCTACCGCAGTGATTTTGCCCATCGTTTGTTGCGGTTGCGCCCGGCGGTGATTCGCGGCAACGCCTCTGAAATCC
TGGCGCTGGCAGGCGCCCCCTCGCGCGGACGCGGCGTCGATAGCCAGGATGACGCGCTGGCGGCGCTACCGGCGGCAGCG
GCGCTGGCGCGCGATAGCGGCGCGGTGGTGGCGATTACCGGCGCCAGCGATTACGTGACCGATGGACATAACAGCTACCG
GATCGACGGCGGCGATCCACTGATGACCCAGGTCGTCGGTACCGGGTGCGCGCTGTCGGCGGTGGCGGCGGCCTTCAGCG
TATTGGACGATCGTCTGACGGCGGTGGCCAGCGCCTGCCGGGTGATGGCGCTGGCGGGTCAGCGGGCGGCGGCGCAGGCC
AGCGGTCCCGGCAGCTTCACGCCCCATTTCCTGGATGCCCTGTATGGCCTTCAGCCCGCCGTCATCGCGGGGGAGGGGGC
ATGA

Upstream 100 bases:

>100_bases
GCTGAGAAATACCCGTATTACCTGATCTGGATAATGCCAGCGTAGGGAAGTCACGGTGTCTGACCGCGCCGCCTTCTTCA
CGCCGGTTGAGAGAGATGCC

Downstream 100 bases:

>100_bases
GCATGAGCGCAGAACGGATTAATGCCCTGACCATCGCCGGTACCGATCCCAGCGGTGGCGCCGGGATCCAGGCCGATCTC
AAAGCCTTTTCCGCGCTGGG

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MLTQPTPLPDALCAQALMTLRQRAPLVHCLTNEVVQSLTANVLLALGAAPAMVVQAEEARQFAALADALLINIGTLYDTR
ANSMLAAIESANLAGTPWVLDPVAVGALRYRSDFAHRLLRLRPAVIRGNASEILALAGAPSRGRGVDSQDDALAALPAAA
ALARDSGAVVAITGASDYVTDGHNSYRIDGGDPLMTQVVGTGCALSAVAAAFSVLDDRLTAVASACRVMALAGQRAAAQA
SGPGSFTPHFLDALYGLQPAVIAGEGA

Sequences:

>Translated_267_residues
MLTQPTPLPDALCAQALMTLRQRAPLVHCLTNEVVQSLTANVLLALGAAPAMVVQAEEARQFAALADALLINIGTLYDTR
ANSMLAAIESANLAGTPWVLDPVAVGALRYRSDFAHRLLRLRPAVIRGNASEILALAGAPSRGRGVDSQDDALAALPAAA
ALARDSGAVVAITGASDYVTDGHNSYRIDGGDPLMTQVVGTGCALSAVAAAFSVLDDRLTAVASACRVMALAGQRAAAQA
SGPGSFTPHFLDALYGLQPAVIAGEGA
>Mature_267_residues
MLTQPTPLPDALCAQALMTLRQRAPLVHCLTNEVVQSLTANVLLALGAAPAMVVQAEEARQFAALADALLINIGTLYDTR
ANSMLAAIESANLAGTPWVLDPVAVGALRYRSDFAHRLLRLRPAVIRGNASEILALAGAPSRGRGVDSQDDALAALPAAA
ALARDSGAVVAITGASDYVTDGHNSYRIDGGDPLMTQVVGTGCALSAVAAAFSVLDDRLTAVASACRVMALAGQRAAAQA
SGPGSFTPHFLDALYGLQPAVIAGEGA

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=257, Percent_Identity=62.2568093385214, Blast_Score=299, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6325042, Length=263, Percent_Identity=32.319391634981, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 27262; Mature: 27262

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQPTPLPDALCAQALMTLRQRAPLVHCLTNEVVQSLTANVLLALGAAPAMVVQAEEAR
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHH
QFAALADALLINIGTLYDTRANSMLAAIESANLAGTPWVLDPVAVGALRYRSDFAHRLLR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHH
LRPAVIRGNASEILALAGAPSRGRGVDSQDDALAALPAAAALARDSGAVVAITGASDYVT
HHHHHHCCCHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEE
DGHNSYRIDGGDPLMTQVVGTGCALSAVAAAFSVLDDRLTAVASACRVMALAGQRAAAQA
CCCCEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
SGPGSFTPHFLDALYGLQPAVIAGEGA
CCCCCCCHHHHHHHHCCCCEEEECCCC
>Mature Secondary Structure
MLTQPTPLPDALCAQALMTLRQRAPLVHCLTNEVVQSLTANVLLALGAAPAMVVQAEEAR
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHH
QFAALADALLINIGTLYDTRANSMLAAIESANLAGTPWVLDPVAVGALRYRSDFAHRLLR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHH
LRPAVIRGNASEILALAGAPSRGRGVDSQDDALAALPAAAALARDSGAVVAITGASDYVT
HHHHHHCCCHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEE
DGHNSYRIDGGDPLMTQVVGTGCALSAVAAAFSVLDDRLTAVASACRVMALAGQRAAAQA
CCCCEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
SGPGSFTPHFLDALYGLQPAVIAGEGA
CCCCCCCHHHHHHHHCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA