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The map label for this gene is thiD [H]

Identifier: 238919083

GI number: 238919083

Start: 1147967

End: 1148572

Strand: Direct

Name: thiD [H]

Synonym: NT01EI_1153

Alternate gene names: 238919083

Gene position: 1147967-1148572 (Clockwise)

Preceding gene: 238919082

Following gene: 238919084

Centisome position: 30.11

GC content: 63.86

Gene sequence:

>606_bases
ATGAGCGCAGAACGGATTAATGCCCTGACCATCGCCGGTACCGATCCCAGCGGTGGCGCCGGGATCCAGGCCGATCTCAA
AGCCTTTTCCGCGCTGGGCGCCTATGGTACCAGCGTCATCACTGCACTGGTGGCCCAGAATACCCGCGGCGTACAGTCGG
TTTACCCTATTGACGCCGGGTTTGTTCAGGAGCAGCTGGACTCCGTGCTGAGCGATGTGCGTATCGACAGCGCGAAAATT
GGCATGCTGTCCAGCGCCGATATCGTCACCTGCGTCGCCGATGCGCTGAAACGCTATCCTTTGCCGTACGTCGTGCTGGA
TACGGTTATGGTGGCCAAAAGCGGCGATGCGCTGCTGGCGCCACAGGCCGTCAGCGCGATTCGCGAGCAGCTGTTGCCGC
AGGTCTCGCTGATCACCCCCAACCTGCCGGAAGCCGCCACGCTGCTGGGGTGTGCGGCGGCAGAGGATGAGGTGGCGATG
GTTGCCCAGGGCGAAGCGCTGCTGGCGCTGGGATGTCAGGCGGTTCTGATGAAGGGGGGACACCTGCGGTGCGGGGAGAG
CCCTGACTGGTTGCTGACCCGCGACGGCGCCGCCGAGCGTTTTTAG

Upstream 100 bases:

>100_bases
CGGCGGCGCAGGCCAGCGGTCCCGGCAGCTTCACGCCCCATTTCCTGGATGCCCTGTATGGCCTTCAGCCCGCCGTCATC
GCGGGGGAGGGGGCATGAGC

Downstream 100 bases:

>100_bases
TGCGGCGCGCGTGGCGACGCGCCATACTCACGGGACGGGCTGCACCCTGTCCGCGGCGCTGGCGGCGCTGCGTCCGCGTC
ATGAAGACTGGCGCGCTACC

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MSAERINALTIAGTDPSGGAGIQADLKAFSALGAYGTSVITALVAQNTRGVQSVYPIDAGFVQEQLDSVLSDVRIDSAKI
GMLSSADIVTCVADALKRYPLPYVVLDTVMVAKSGDALLAPQAVSAIREQLLPQVSLITPNLPEAATLLGCAAAEDEVAM
VAQGEALLALGCQAVLMKGGHLRCGESPDWLLTRDGAAERF

Sequences:

>Translated_201_residues
MSAERINALTIAGTDPSGGAGIQADLKAFSALGAYGTSVITALVAQNTRGVQSVYPIDAGFVQEQLDSVLSDVRIDSAKI
GMLSSADIVTCVADALKRYPLPYVVLDTVMVAKSGDALLAPQAVSAIREQLLPQVSLITPNLPEAATLLGCAAAEDEVAM
VAQGEALLALGCQAVLMKGGHLRCGESPDWLLTRDGAAERF
>Mature_200_residues
SAERINALTIAGTDPSGGAGIQADLKAFSALGAYGTSVITALVAQNTRGVQSVYPIDAGFVQEQLDSVLSDVRIDSAKIG
MLSSADIVTCVADALKRYPLPYVVLDTVMVAKSGDALLAPQAVSAIREQLLPQVSLITPNLPEAATLLGCAAAEDEVAMV
AQGEALLALGCQAVLMKGGHLRCGESPDWLLTRDGAAERF

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=198, Percent_Identity=71.2121212121212, Blast_Score=275, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6324517, Length=199, Percent_Identity=36.6834170854271, Blast_Score=105, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324997, Length=204, Percent_Identity=36.7647058823529, Blast_Score=104, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6325378, Length=206, Percent_Identity=30.0970873786408, Blast_Score=88, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 20770; Mature: 20639

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAERINALTIAGTDPSGGAGIQADLKAFSALGAYGTSVITALVAQNTRGVQSVYPIDAG
CCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHH
FVQEQLDSVLSDVRIDSAKIGMLSSADIVTCVADALKRYPLPYVVLDTVMVAKSGDALLA
HHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEC
PQAVSAIREQLLPQVSLITPNLPEAATLLGCAAAEDEVAMVAQGEALLALGCQAVLMKGG
HHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCC
HLRCGESPDWLLTRDGAAERF
CEECCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
SAERINALTIAGTDPSGGAGIQADLKAFSALGAYGTSVITALVAQNTRGVQSVYPIDAG
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHH
FVQEQLDSVLSDVRIDSAKIGMLSSADIVTCVADALKRYPLPYVVLDTVMVAKSGDALLA
HHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEC
PQAVSAIREQLLPQVSLITPNLPEAATLLGCAAAEDEVAMVAQGEALLALGCQAVLMKGG
HHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCC
HLRCGESPDWLLTRDGAAERF
CEECCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]