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The map label for this gene is ushA [H]

Identifier: 238919064

GI number: 238919064

Start: 1129532

End: 1131190

Strand: Direct

Name: ushA [H]

Synonym: NT01EI_1132

Alternate gene names: 238919064

Gene position: 1129532-1131190 (Clockwise)

Preceding gene: 238919063

Following gene: 238919070

Centisome position: 29.63

GC content: 59.67

Gene sequence:

>1659_bases
ATGAAATTTACCTATCGGGGTGCCGCCTGCGCGATAGCACTAACGCTGGCGCTGACGCCAGGCTGGTCGCTGGCCTGGGA
AAAAGATAAAACCTATAACCTGACCATTCTGCATACCAACGATCACCACGGCCACTTCTGGCAGAGCGAGCAGGGCGAAT
ATGGTCTGGCTGCCCAGAAAACGCTGGTGGATCGCCTGCGTGCCGAGATTGCCGCTGAGGGCGGCAGCGTGCTGCTGCTG
TCGGGGGGGGATATCAATACCGGAGTTCCGGAGTCCGATCTGCAGGATGCCGAGCCCGATTTTCGCGGTATGAATCTGGT
GGGCTACGATGCAATGGCCATCGGCAACCACGAGTTCGACAATCCGCTGAACGTGCTGCGTCAGCAGCAGAAGTGGGCCG
ACTTCCCGCTGCTGTCGGCCAATATCTATGCGCAAGATAGTGGTAAGCGCCTGTTTCAGCCCTATCAGATGTTCGATCGC
CAGGGGATCCGCATTGCGGTGATCGGCCTGACCACCGACGATACGGTCAAGATAGGTAACCCTGAATACCTGAAGAATAT
CGAGTTCCGCCCCCCGGCGGCGGAGGCCAAGGGCGTGGTAGAGCAGCTGCGGGCCAACGAGAAGCCGGACGTGGTGATCG
CCGCGACGCACATGGGCCACTATGACAACGGCAAGCATGGCTCCAACGCCCCGGGCGATGTTGAAATGGCGCGCGCGCTG
CCGGCGGGCTACCTCGACATGATCGTTGGCGGCCACTCGCAGGATCCGGTGTGCATGGCACAGGAAAACCGTAAGCAGGC
GAATTATGTCCCCGGTACGCCTTGTGCGCCGGATCGCCAGAACGGGACCTGGATCGTTCAGGCTCATGAATGGGGTAAAT
ATGTTGGACGGGCCGACTTTACCTTCCGTAACGGGGAGCTGACCCTGCAGCGCTACCAGCTGATCCCGGTGAATCTCAAC
CGGCAGGAGAAGCAGGCCGACGGTTCGGAGACGCGGATCTATTACACCGAGGAGATCCCGCAGGATAAGGTGATGCTGAA
GCTGCTGGCGCCGTATCAGGATCAGGGGCAGGCCGCGCTGGCGGTGAAAATCGGCAGTGTAGAGGGGCGTCTGGAGGGGG
ATCGCAGCAGGGTCCGTTTTGAGCAGACCAACATGGGGCGCCTGCTACTGGCAGCGCAGATGGAGCGAACCCATGCCGAC
CTGGGCGTAATGAGCGGTGGCGGGGTGCGCGACTCGATAGACAGCGGTGACATCAGCTATAAGGATGTGCTTAAGGTGCA
GCCGTTCGGTAATGCCGTAGTCTATGTCGACATGAACGGTGCGGAGCTGGAGAAATACCTGAGCGCCGTCGCCAATAAAA
AGCCCAACTCCGGCGCCTATGCGCAGTTTGCCAATCTGAGCCTGACGGCGGACGGCAAGCAGGGCGTCAGCGACGTGAAG
ATTGGCGGGAAACCGCTGCAGGCTGACCGGATCTATCGCGTAGCGTTGCTGAGCTTTAATGCCAGCGGCGGCGATGATTA
CCCCAATGTGACCCAGTATCCGGGCTACGTGAACACCGGTTTTATTGACGCCGAAGTGCTGAAGGACTATATCCAGCGCC
ATTCACCGCTCAAGGCGGCGGACTATCAGCCGAACGGCGAGATCGTGTATCGCAATTAA

Upstream 100 bases:

>100_bases
CAGAATCACCGACTGATTCAGGTTAAGATAGGGATGTTCGCCGCCGAGCCTCCCCGGGGCTGGCATGAGTGGCGCGTTAT
TGTGACAAGGAGAGAGGGCA

Downstream 100 bases:

>100_bases
GCGTCATTTCCCACGCCGTGCGTAGCGGTATTTATCTCTGTAACGGCATGCGCGGCATGCCGTTTTTGATCGCCGCGAAG
CCGGTCTCAGGCCGCGTCGC

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: UDP-sugar hydrolase; UDP-sugar diphosphatase; UDP-sugar pyrophosphatase; 5'-nucleotidase; 5'-NT [H]

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MKFTYRGAACAIALTLALTPGWSLAWEKDKTYNLTILHTNDHHGHFWQSEQGEYGLAAQKTLVDRLRAEIAAEGGSVLLL
SGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFDNPLNVLRQQQKWADFPLLSANIYAQDSGKRLFQPYQMFDR
QGIRIAVIGLTTDDTVKIGNPEYLKNIEFRPPAAEAKGVVEQLRANEKPDVVIAATHMGHYDNGKHGSNAPGDVEMARAL
PAGYLDMIVGGHSQDPVCMAQENRKQANYVPGTPCAPDRQNGTWIVQAHEWGKYVGRADFTFRNGELTLQRYQLIPVNLN
RQEKQADGSETRIYYTEEIPQDKVMLKLLAPYQDQGQAALAVKIGSVEGRLEGDRSRVRFEQTNMGRLLLAAQMERTHAD
LGVMSGGGVRDSIDSGDISYKDVLKVQPFGNAVVYVDMNGAELEKYLSAVANKKPNSGAYAQFANLSLTADGKQGVSDVK
IGGKPLQADRIYRVALLSFNASGGDDYPNVTQYPGYVNTGFIDAEVLKDYIQRHSPLKAADYQPNGEIVYRN

Sequences:

>Translated_552_residues
MKFTYRGAACAIALTLALTPGWSLAWEKDKTYNLTILHTNDHHGHFWQSEQGEYGLAAQKTLVDRLRAEIAAEGGSVLLL
SGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFDNPLNVLRQQQKWADFPLLSANIYAQDSGKRLFQPYQMFDR
QGIRIAVIGLTTDDTVKIGNPEYLKNIEFRPPAAEAKGVVEQLRANEKPDVVIAATHMGHYDNGKHGSNAPGDVEMARAL
PAGYLDMIVGGHSQDPVCMAQENRKQANYVPGTPCAPDRQNGTWIVQAHEWGKYVGRADFTFRNGELTLQRYQLIPVNLN
RQEKQADGSETRIYYTEEIPQDKVMLKLLAPYQDQGQAALAVKIGSVEGRLEGDRSRVRFEQTNMGRLLLAAQMERTHAD
LGVMSGGGVRDSIDSGDISYKDVLKVQPFGNAVVYVDMNGAELEKYLSAVANKKPNSGAYAQFANLSLTADGKQGVSDVK
IGGKPLQADRIYRVALLSFNASGGDDYPNVTQYPGYVNTGFIDAEVLKDYIQRHSPLKAADYQPNGEIVYRN
>Mature_552_residues
MKFTYRGAACAIALTLALTPGWSLAWEKDKTYNLTILHTNDHHGHFWQSEQGEYGLAAQKTLVDRLRAEIAAEGGSVLLL
SGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFDNPLNVLRQQQKWADFPLLSANIYAQDSGKRLFQPYQMFDR
QGIRIAVIGLTTDDTVKIGNPEYLKNIEFRPPAAEAKGVVEQLRANEKPDVVIAATHMGHYDNGKHGSNAPGDVEMARAL
PAGYLDMIVGGHSQDPVCMAQENRKQANYVPGTPCAPDRQNGTWIVQAHEWGKYVGRADFTFRNGELTLQRYQLIPVNLN
RQEKQADGSETRIYYTEEIPQDKVMLKLLAPYQDQGQAALAVKIGSVEGRLEGDRSRVRFEQTNMGRLLLAAQMERTHAD
LGVMSGGGVRDSIDSGDISYKDVLKVQPFGNAVVYVDMNGAELEKYLSAVANKKPNSGAYAQFANLSLTADGKQGVSDVK
IGGKPLQADRIYRVALLSFNASGGDDYPNVTQYPGYVNTGFIDAEVLKDYIQRHSPLKAADYQPNGEIVYRN

Specific function: Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=587, Percent_Identity=26.0647359454855, Blast_Score=128, Evalue=2e-29,
Organism=Escherichia coli, GI1786687, Length=552, Percent_Identity=69.5652173913043, Blast_Score=816, Evalue=0.0,
Organism=Escherichia coli, GI1790658, Length=466, Percent_Identity=19.9570815450644, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922446, Length=525, Percent_Identity=26.0952380952381, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24654424, Length=525, Percent_Identity=26.0952380952381, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19922444, Length=556, Percent_Identity=23.9208633093525, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28573524, Length=579, Percent_Identity=24.006908462867, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI221329836, Length=565, Percent_Identity=21.4159292035398, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24641187, Length=563, Percent_Identity=22.0248667850799, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.6.1.45; =3.1.3.5 [H]

Molecular weight: Translated: 60851; Mature: 60851

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTYRGAACAIALTLALTPGWSLAWEKDKTYNLTILHTNDHHGHFWQSEQGEYGLAAQK
CCEEECCHHEEEEEEEEECCCCCEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHH
TLVDRLRAEIAAEGGSVLLLSGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFD
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCCCC
NPLNVLRQQQKWADFPLLSANIYAQDSGKRLFQPYQMFDRQGIRIAVIGLTTDDTVKIGN
CHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCEEEEEEECCCCEEECCC
PEYLKNIEFRPPAAEAKGVVEQLRANEKPDVVIAATHMGHYDNGKHGSNAPGDVEMARAL
HHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHC
PAGYLDMIVGGHSQDPVCMAQENRKQANYVPGTPCAPDRQNGTWIVQAHEWGKYVGRADF
CCCHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCE
TFRNGELTLQRYQLIPVNLNRQEKQADGSETRIYYTEEIPQDKVMLKLLAPYQDQGQAAL
EEECCCEEEEEEEEEEECCCCHHHHCCCCCEEEEECCCCCCHHHHHHEECCCCCCCCEEE
AVKIGSVEGRLEGDRSRVRFEQTNMGRLLLAAQMERTHADLGVMSGGGVRDSIDSGDISY
EEEEECCCCCCCCCCHHEEEECCCCCEEEEEHHHHHHHHCCEEECCCCCCCCCCCCCCCH
KDVLKVQPFGNAVVYVDMNGAELEKYLSAVANKKPNSGAYAQFANLSLTADGKQGVSDVK
HHEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCEEE
IGGKPLQADRIYRVALLSFNASGGDDYPNVTQYPGYVNTGFIDAEVLKDYIQRHSPLKAA
ECCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHCCCCCCC
DYQPNGEIVYRN
CCCCCCCEEEEC
>Mature Secondary Structure
MKFTYRGAACAIALTLALTPGWSLAWEKDKTYNLTILHTNDHHGHFWQSEQGEYGLAAQK
CCEEECCHHEEEEEEEEECCCCCEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHH
TLVDRLRAEIAAEGGSVLLLSGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFD
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCCCC
NPLNVLRQQQKWADFPLLSANIYAQDSGKRLFQPYQMFDRQGIRIAVIGLTTDDTVKIGN
CHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCEEEEEEECCCCEEECCC
PEYLKNIEFRPPAAEAKGVVEQLRANEKPDVVIAATHMGHYDNGKHGSNAPGDVEMARAL
HHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHC
PAGYLDMIVGGHSQDPVCMAQENRKQANYVPGTPCAPDRQNGTWIVQAHEWGKYVGRADF
CCCHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCE
TFRNGELTLQRYQLIPVNLNRQEKQADGSETRIYYTEEIPQDKVMLKLLAPYQDQGQAAL
EEECCCEEEEEEEEEEECCCCHHHHCCCCCEEEEECCCCCCHHHHHHEECCCCCCCCEEE
AVKIGSVEGRLEGDRSRVRFEQTNMGRLLLAAQMERTHADLGVMSGGGVRDSIDSGDISY
EEEEECCCCCCCCCCHHEEEECCCCCEEEEEHHHHHHHHCCEEECCCCCCCCCCCCCCCH
KDVLKVQPFGNAVVYVDMNGAELEKYLSAVANKKPNSGAYAQFANLSLTADGKQGVSDVK
HHEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCEEE
IGGKPLQADRIYRVALLSFNASGGDDYPNVTQYPGYVNTGFIDAEVLKDYIQRHSPLKAA
ECCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHCCCCCCC
DYQPNGEIVYRN
CCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA