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The map label for this gene is lpfB [H]

Identifier: 238919061

GI number: 238919061

Start: 1124963

End: 1125649

Strand: Direct

Name: lpfB [H]

Synonym: NT01EI_1129

Alternate gene names: 238919061

Gene position: 1124963-1125649 (Clockwise)

Preceding gene: 238919060

Following gene: 238919062

Centisome position: 29.51

GC content: 50.36

Gene sequence:

>687_bases
ATGAACCGAATCGTTGCCGTATTATTACTGGTGAGCACTTTTACTGCCCATGCAGGAATTCAGGTTGATGCTACGCGTGT
TATTTATAATGGCGATGAAAAGTCAGCGTCTCTGCCTATCCACAATGACAGTGTGGATGCCTATATGGTGCAGACATGGC
TGGATAACGGGGATAGCACCAAGGTAGAGAGTAAGTTACCGATGGTGGTGGTACCACCGATTGTTAAACTGGATGCGCAA
AAATCGGCAATTTTACGTTTTATCTATTCCGGGCAGGGTTTTCTGCAGGATCAGGAAACACTGCTGTGGGTGAACGTACA
GGAAATCCCGCCAACGCCAAAGCAAGAAAATGTATTACAACTTGCTGTACGTACGCGGATTAAGCTGTTTTATCGCCCGG
GTTCGCTGCATACCACGCTGGATGAACAGGTTCGTAAACTGCGCTGGCAGAAAGCGGGTCATCAGTTACTGGCCATCAAC
GACGGGCCGCTGCACATCACCTTTGGCTCTGTACAGATGCAGGGCAACAACGGTAAGCCCATTGCGGTAGATACCAATAT
GGTCAACCCCCACAGCCGCCTGGCGATCGCCATTCCGGCGGGAGCTCACGTCGGAAATACAGTCTCCTTCAGCTACATCA
ATGACTTTGGGGGCCGGACTGAGGTTAAGGACGCGACAGTATACTGA

Upstream 100 bases:

>100_bases
TACCGCCGGTCTGGCCAACGCGACCAGTCCGTTCACCATCGAATATAAATAATTATTCGTCGGAATATTCGGCCAGGGAT
GGTTCGTATAGAGGAATAAG

Downstream 100 bases:

>100_bases
CGGAGAGAAGCGGGATGGCGCAACACCAACGGACAGGATCACTGAAGACAGAGCCGACACGCTGGATGTGCCTGGCATTT
GCCGCCGGTAGCCTGTCCCT

Product: fimbrial chaperon protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MNRIVAVLLLVSTFTAHAGIQVDATRVIYNGDEKSASLPIHNDSVDAYMVQTWLDNGDSTKVESKLPMVVVPPIVKLDAQ
KSAILRFIYSGQGFLQDQETLLWVNVQEIPPTPKQENVLQLAVRTRIKLFYRPGSLHTTLDEQVRKLRWQKAGHQLLAIN
DGPLHITFGSVQMQGNNGKPIAVDTNMVNPHSRLAIAIPAGAHVGNTVSFSYINDFGGRTEVKDATVY

Sequences:

>Translated_228_residues
MNRIVAVLLLVSTFTAHAGIQVDATRVIYNGDEKSASLPIHNDSVDAYMVQTWLDNGDSTKVESKLPMVVVPPIVKLDAQ
KSAILRFIYSGQGFLQDQETLLWVNVQEIPPTPKQENVLQLAVRTRIKLFYRPGSLHTTLDEQVRKLRWQKAGHQLLAIN
DGPLHITFGSVQMQGNNGKPIAVDTNMVNPHSRLAIAIPAGAHVGNTVSFSYINDFGGRTEVKDATVY
>Mature_228_residues
MNRIVAVLLLVSTFTAHAGIQVDATRVIYNGDEKSASLPIHNDSVDAYMVQTWLDNGDSTKVESKLPMVVVPPIVKLDAQ
KSAILRFIYSGQGFLQDQETLLWVNVQEIPPTPKQENVLQLAVRTRIKLFYRPGSLHTTLDEQVRKLRWQKAGHQLLAIN
DGPLHITFGSVQMQGNNGKPIAVDTNMVNPHSRLAIAIPAGAHVGNTVSFSYINDFGGRTEVKDATVY

Specific function: Required for the biogenesis of long polar fimbria; binds and interact with lpfA [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=232, Percent_Identity=33.1896551724138, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1790771, Length=222, Percent_Identity=31.981981981982, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789532, Length=228, Percent_Identity=29.3859649122807, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787171, Length=222, Percent_Identity=31.0810810810811, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1786743, Length=231, Percent_Identity=29.4372294372294, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786936, Length=221, Percent_Identity=30.7692307692308, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1788428, Length=222, Percent_Identity=27.9279279279279, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI87082203, Length=223, Percent_Identity=32.7354260089686, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788677, Length=207, Percent_Identity=28.5024154589372, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081806, Length=228, Percent_Identity=25.4385964912281, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 25182; Mature: 25182

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIVAVLLLVSTFTAHAGIQVDATRVIYNGDEKSASLPIHNDSVDAYMVQTWLDNGDST
CCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCC
KVESKLPMVVVPPIVKLDAQKSAILRFIYSGQGFLQDQETLLWVNVQEIPPTPKQENVLQ
EECCCCCEEEECCCEEECCCHHHEEEEEECCCCCEECCCEEEEEEEECCCCCCCCCHHHH
LAVRTRIKLFYRPGSLHTTLDEQVRKLRWQKAGHQLLAINDGPLHITFGSVQMQGNNGKP
HEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEEECCCCCE
IAVDTNMVNPHSRLAIAIPAGAHVGNTVSFSYINDFGGRTEVKDATVY
EEEECCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRIVAVLLLVSTFTAHAGIQVDATRVIYNGDEKSASLPIHNDSVDAYMVQTWLDNGDST
CCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCC
KVESKLPMVVVPPIVKLDAQKSAILRFIYSGQGFLQDQETLLWVNVQEIPPTPKQENVLQ
EECCCCCEEEECCCEEECCCHHHEEEEEECCCCCEECCCEEEEEEEECCCCCCCCCHHHH
LAVRTRIKLFYRPGSLHTTLDEQVRKLRWQKAGHQLLAINDGPLHITFGSVQMQGNNGKP
HEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEEECCCCCE
IAVDTNMVNPHSRLAIAIPAGAHVGNTVSFSYINDFGGRTEVKDATVY
EEEECCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7721701; 11677609 [H]