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The map label for this gene is thiI

Identifier: 238918997

GI number: 238918997

Start: 1060716

End: 1062164

Strand: Direct

Name: thiI

Synonym: NT01EI_1065

Alternate gene names: 238918997

Gene position: 1060716-1062164 (Clockwise)

Preceding gene: 238918993

Following gene: 238919005

Centisome position: 27.82

GC content: 58.25

Gene sequence:

>1449_bases
ATGAAGTTTATCATTAAATTATTCCCGGAAATCACTATTAAAAGCCAGTCTGTACGCCTGCGCTTTATTAAAATCTTGAC
CGGGAATATCCGCAACGTGTTGAAACCGTTGGTTGAAGATGTGGCGGTGACGCGCCATTGGGATCATATCGAAGTTCGGG
CCAAAGAGGAAAGCCGCCGCGACCTGGTTCGTGATGCCTTAACCCGGATCCCGGGTATTCATCACATTTTAGAGGTGGAG
GATCATCCCTACACCGACATGCACGATATCTTTGAACAGACGCTGGCGATGTACCGTGAGCAGCTGGAAGAGAAAACCTT
TTGCGTGCGCGTTAAGCGCCGCGGCAAACATACGTTTACCTCAATTGAGGTGGAGCGCTATGTCGGCGGCGGTCTGAATC
AGAATATCCCCAGTGCCCGGGTTCGTCTGAACCACCCCGAGGTGACGGTTAACCTGGAGATTGAAGACGATCGTTTGCTG
CTGGTGCGCGGCCGCTTTGAGGGACAGGGGGGGTACCCTATCGGTACCCAGGAGGATGTTCTGTCGCTGATCTCCGGCGG
CTTTGACTCCGGGGTTTCCAGCTATATGCTGATGCGCCGCGGCTGCCGGGTGCACTACTGCTTCTTCAACCTGGGCGGCT
CTGCGCATGAAATCGGCGTGAAGCAGGTGGCACACTATCTGTGGAACCGGTTCGGCAGCTCGCACCGGGTACGCTTTGTC
GCCATTGACTTTGCACCGGTGGTCGGTGAGATCCTGGAGAAGGTGGACGACGGCCAGATGGGGGTGGTGCTGAAGCGCAT
GATGGTGCGTGCCGCGTCTCGGGTCGCCGAACGCTATGGTATTCAGGCGCTGGTGACCGGGGAGGCGCTGGGACAGGTCT
CCAGCCAGACCCTGACCAACCTGCGTCTGATCGACGGCGTGAGCGATACGCTGGTGCTGCGTCCGCTGATTGCCCATGAC
AAAGAGCATATCATCCGCCTGGCGCGCAACATCGGTACCGAGGATTTTGCCAAGACCATGCCGGAATTCTGCGGCGTGAT
CTCGAAGAGCCCGACCGTGAAGGCGGTGAAGGCGAGGATTGAGGCCGAAGAGGAGCACTTCGATTTCGCCATTCTGGATC
GTGTGGTCGACGAGGCGCAGACCATGGACATTCGCGACATCGCGCGTCAGAGCGAGGCCGACGTCGTCGAAGTCGAGACC
ATCGAATCGCTGGCCGAGGGCGATGTGGTCCTGGATATCCGCGCTCCCGATGAGCAGGAGGCGAAGCCGTTGCAGCTGGC
AGACAGCGAGGTGATCTGCCTGCCGTTCTATAAGCTGGCCAATGCCTTCGGCGATCTGGATCAAGTCCGTCGTTACGTGC
TGTACTGCGATCGTGGGGTGATGAGCCGCCTGCAGGCACTCTACCTGCGCGAGCAGGGCTACGACAACGTCCGCGTCTAC
CGCCCTTAA

Upstream 100 bases:

>100_bases
TGCGCGCCCTGTGCTCGGCACGGCGCATTCTGCAGAAGACGCGTCCGTGCAGTGTAAGCCCTTTTAACGCATATCTCGAC
GACTATTAGCCACGACCGCC

Downstream 100 bases:

>100_bases
GCGTTTCCCTCCGCCATGTTGCCGCTCAGCCTCGTTTGCAGGCTGAGCGGTAACGTCAAACCATACGCCTCCAGCAGCGT
CTCATCGCGCAGGATCTGCG

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKPLVEDVAVTRHWDHIEVRAKEESRRDLVRDALTRIPGIHHILEVE
DHPYTDMHDIFEQTLAMYREQLEEKTFCVRVKRRGKHTFTSIEVERYVGGGLNQNIPSARVRLNHPEVTVNLEIEDDRLL
LVRGRFEGQGGYPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGSAHEIGVKQVAHYLWNRFGSSHRVRFV
AIDFAPVVGEILEKVDDGQMGVVLKRMMVRAASRVAERYGIQALVTGEALGQVSSQTLTNLRLIDGVSDTLVLRPLIAHD
KEHIIRLARNIGTEDFAKTMPEFCGVISKSPTVKAVKARIEAEEEHFDFAILDRVVDEAQTMDIRDIARQSEADVVEVET
IESLAEGDVVLDIRAPDEQEAKPLQLADSEVICLPFYKLANAFGDLDQVRRYVLYCDRGVMSRLQALYLREQGYDNVRVY
RP

Sequences:

>Translated_482_residues
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKPLVEDVAVTRHWDHIEVRAKEESRRDLVRDALTRIPGIHHILEVE
DHPYTDMHDIFEQTLAMYREQLEEKTFCVRVKRRGKHTFTSIEVERYVGGGLNQNIPSARVRLNHPEVTVNLEIEDDRLL
LVRGRFEGQGGYPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGSAHEIGVKQVAHYLWNRFGSSHRVRFV
AIDFAPVVGEILEKVDDGQMGVVLKRMMVRAASRVAERYGIQALVTGEALGQVSSQTLTNLRLIDGVSDTLVLRPLIAHD
KEHIIRLARNIGTEDFAKTMPEFCGVISKSPTVKAVKARIEAEEEHFDFAILDRVVDEAQTMDIRDIARQSEADVVEVET
IESLAEGDVVLDIRAPDEQEAKPLQLADSEVICLPFYKLANAFGDLDQVRRYVLYCDRGVMSRLQALYLREQGYDNVRVY
RP
>Mature_482_residues
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKPLVEDVAVTRHWDHIEVRAKEESRRDLVRDALTRIPGIHHILEVE
DHPYTDMHDIFEQTLAMYREQLEEKTFCVRVKRRGKHTFTSIEVERYVGGGLNQNIPSARVRLNHPEVTVNLEIEDDRLL
LVRGRFEGQGGYPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGSAHEIGVKQVAHYLWNRFGSSHRVRFV
AIDFAPVVGEILEKVDDGQMGVVLKRMMVRAASRVAERYGIQALVTGEALGQVSSQTLTNLRLIDGVSDTLVLRPLIAHD
KEHIIRLARNIGTEDFAKTMPEFCGVISKSPTVKAVKARIEAEEEHFDFAILDRVVDEAQTMDIRDIARQSEADVVEVET
IESLAEGDVVLDIRAPDEQEAKPLQLADSEVICLPFYKLANAFGDLDQVRRYVLYCDRGVMSRLQALYLREQGYDNVRVY
RP

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=482, Percent_Identity=79.4605809128631, Blast_Score=787, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_EDWI9 (C5BCI2)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002932511.1
- ProteinModelPortal:   C5BCI2
- GeneID:   7960650
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1065
- OMA:   GYPIGTQ
- ProtClustDB:   PRK01269
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.250.10
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP; SSF52821 Rhodanese-like

EC number: =2.8.1.4

Molecular weight: Translated: 54872; Mature: 54872

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50206 RHODANESE_3; PS51165 THUMP

Important sites: ACT_SITE 456-456 BINDING 265-265 BINDING 287-287 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKPLVEDVAVTRHWDHIEVRAKEESRR
CCCCEEECCCCEEECCHHEEEEEHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHHH
DLVRDALTRIPGIHHILEVEDHPYTDMHDIFEQTLAMYREQLEEKTFCVRVKRRGKHTFT
HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
SIEVERYVGGGLNQNIPSARVRLNHPEVTVNLEIEDDRLLLVRGRFEGQGGYPIGTQEDV
EEEEHHHHCCCCCCCCCCCEEEECCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCHHHH
LSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGSAHEIGVKQVAHYLWNRFGSSHRVRFV
HHHHHCCCCHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
AIDFAPVVGEILEKVDDGQMGVVLKRMMVRAASRVAERYGIQALVTGEALGQVSSQTLTN
EEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHH
LRLIDGVSDTLVLRPLIAHDKEHIIRLARNIGTEDFAKTMPEFCGVISKSPTVKAVKARI
HHEECCCCHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EAEEEHFDFAILDRVVDEAQTMDIRDIARQSEADVVEVETIESLAEGDVVLDIRAPDEQE
HCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEHHHHHHHHCCCEEEEEECCCCCC
AKPLQLADSEVICLPFYKLANAFGDLDQVRRYVLYCDRGVMSRLQALYLREQGYDNVRVY
CCCCEECCCCEEEEHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEE
RP
CC
>Mature Secondary Structure
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKPLVEDVAVTRHWDHIEVRAKEESRR
CCCCEEECCCCEEECCHHEEEEEHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHHH
DLVRDALTRIPGIHHILEVEDHPYTDMHDIFEQTLAMYREQLEEKTFCVRVKRRGKHTFT
HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
SIEVERYVGGGLNQNIPSARVRLNHPEVTVNLEIEDDRLLLVRGRFEGQGGYPIGTQEDV
EEEEHHHHCCCCCCCCCCCEEEECCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCHHHH
LSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGSAHEIGVKQVAHYLWNRFGSSHRVRFV
HHHHHCCCCHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
AIDFAPVVGEILEKVDDGQMGVVLKRMMVRAASRVAERYGIQALVTGEALGQVSSQTLTN
EEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHH
LRLIDGVSDTLVLRPLIAHDKEHIIRLARNIGTEDFAKTMPEFCGVISKSPTVKAVKARI
HHEECCCCHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EAEEEHFDFAILDRVVDEAQTMDIRDIARQSEADVVEVETIESLAEGDVVLDIRAPDEQE
HCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEHHHHHHHHCCCEEEEEECCCCCC
AKPLQLADSEVICLPFYKLANAFGDLDQVRRYVLYCDRGVMSRLQALYLREQGYDNVRVY
CCCCEECCCCEEEEHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEE
RP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA