Click here to switch to the map view.

The map label for this gene is nusB

Identifier: 238918991

GI number: 238918991

Start: 1055329

End: 1055748

Strand: Direct

Name: nusB

Synonym: NT01EI_1058

Alternate gene names: 238918991

Gene position: 1055329-1055748 (Clockwise)

Preceding gene: 238918990

Following gene: 238918992

Centisome position: 27.68

GC content: 53.57

Gene sequence:

>420_bases
GTGAAACCTGCTGCCCGTCGCCGCGCCCGTGAGTGTGCTGTCCAGGCACTCTACTCTTGGCAGATCTCTAAAAATGACAT
CGCTGATGTCGAATACCAGTTTCTGTCAGAGCAGGACGTGAAAGATGTTGACGTTAACTATTTCCGTGAGCTGTTGTCCG
GTGTGGCCAGCAATGCGGAGTATCTGGATGGCCTGATGGCGCCCGTGCTGTCACGTCAGCTGGATGAGCTGGGACAGGTT
GAGAGAGCTATCCTGCGCGTGTCCATCTATGAGCTGAGCAAGCGCCAGGATGTCCCTTATAAAGTGGCCATCAATGAAGG
CATAGAGCTGGCAAAAATCTTTGGCGCCGAAGACAGCCACAAGTTTGTCAACGGCGTACTGGATAAGGTTGCGCCACAGG
TGCGTCCGAACCGTAAATAA

Upstream 100 bases:

>100_bases
CAAAGCGGGTAACAAAGGTGCGGAAGCCGCGCTGACCGCGCTCGAAATGATCAATGTACTTAAAGCCATTAAAGCCTGAT
TTATTAGTAAGGGGAATTCC

Downstream 100 bases:

>100_bases
TCATTGCGGGTCGTCCGCCCGGCCTTATGGGGCCTGACAGCTATGCCACAGGCCGGTTCTCCGGCCTTTTTTTCGGCTGT
GCGAACGACCAGATCGCAGG

Product: transcription antitermination factor NusB

Products: NA

Alternate protein names: Protein nusB

Number of amino acids: Translated: 139; Mature: 139

Protein sequence:

>139_residues
MKPAARRRARECAVQALYSWQISKNDIADVEYQFLSEQDVKDVDVNYFRELLSGVASNAEYLDGLMAPVLSRQLDELGQV
ERAILRVSIYELSKRQDVPYKVAINEGIELAKIFGAEDSHKFVNGVLDKVAPQVRPNRK

Sequences:

>Translated_139_residues
MKPAARRRARECAVQALYSWQISKNDIADVEYQFLSEQDVKDVDVNYFRELLSGVASNAEYLDGLMAPVLSRQLDELGQV
ERAILRVSIYELSKRQDVPYKVAINEGIELAKIFGAEDSHKFVNGVLDKVAPQVRPNRK
>Mature_139_residues
MKPAARRRARECAVQALYSWQISKNDIADVEYQFLSEQDVKDVDVNYFRELLSGVASNAEYLDGLMAPVLSRQLDELGQV
ERAILRVSIYELSKRQDVPYKVAINEGIELAKIFGAEDSHKFVNGVLDKVAPQVRPNRK

Specific function: Involved in the transcription termination process

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family

Homologues:

Organism=Escherichia coli, GI1786618, Length=139, Percent_Identity=82.7338129496403, Blast_Score=229, Evalue=6e-62,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSB_EDWI9 (C5BCH6)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002932505.1
- GeneID:   7959441
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1058
- OMA:   SHNDIAD
- ProtClustDB:   CLSK2812936
- HAMAP:   MF_00073
- InterPro:   IPR011605
- InterPro:   IPR006027
- Gene3D:   G3DSA:1.10.940.10
- TIGRFAMs:   TIGR01951

Pfam domain/function: PF01029 NusB; SSF48013 NusB_RsmB_TIM44

EC number: NA

Molecular weight: Translated: 15784; Mature: 15784

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPAARRRARECAVQALYSWQISKNDIADVEYQFLSEQDVKDVDVNYFRELLSGVASNAE
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHH
YLDGLMAPVLSRQLDELGQVERAILRVSIYELSKRQDVPYKVAINEGIELAKIFGAEDSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCHH
KFVNGVLDKVAPQVRPNRK
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKPAARRRARECAVQALYSWQISKNDIADVEYQFLSEQDVKDVDVNYFRELLSGVASNAE
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHH
YLDGLMAPVLSRQLDELGQVERAILRVSIYELSKRQDVPYKVAINEGIELAKIFGAEDSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCHH
KFVNGVLDKVAPQVRPNRK
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA