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The map label for this gene is ribH

Identifier: 238918990

GI number: 238918990

Start: 1054837

End: 1055307

Strand: Direct

Name: ribH

Synonym: NT01EI_1057

Alternate gene names: 238918990

Gene position: 1054837-1055307 (Clockwise)

Preceding gene: 238918989

Following gene: 238918991

Centisome position: 27.67

GC content: 58.17

Gene sequence:

>471_bases
ATGAAAACGATTCAAGGCGTTGTTGCTGCTCCCCAGGCGCGTGTTGCCATCGCGATCTCGCGCTTTAACCATTTCATCAA
TGACAGTTTGCTGGAAGGGGCGATCGACGCGCTGAAGCGTATTGGTCAGGTTTCTGACGAAAATATTACCGTCGTGTGGG
TGCCGGGCGCCTATGAGCTGCCGTTGACCGTACGCGCGCTGGCGTCCTCTGGCCGTTACGACGCCGTTGTGGCGCTGGGC
ACCGTGATCCGCGGCGGCACTGCCCATTTTGAATTCGTTGCCGGTGAGTGCAGCTCCGGTCTGGCCAGCGTTGCGCTGAA
TACCGACGTACCGGTCGCCTTTGGCGTGCTGACCACTGAAACCATCGAACAAGCCATTGATCGCGCCGGGGCCAAAGCGG
GTAACAAAGGTGCGGAAGCCGCGCTGACCGCGCTCGAAATGATCAATGTACTTAAAGCCATTAAAGCCTGA

Upstream 100 bases:

>100_bases
CACAGCCGCCGAAATGACAATGGATCTGAGCAGAAGTGCAAAGAGTATGATAGAATCCGCCCCCCTGCGGGGTATAACTG
ACGTATGTTAAGGAAACCGT

Downstream 100 bases:

>100_bases
TTTATTAGTAAGGGGAATTCCGTGAAACCTGCTGCCCGTCGCCGCGCCCGTGAGTGTGCTGTCCAGGCACTCTACTCTTG
GCAGATCTCTAAAAATGACA

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKTIQGVVAAPQARVAIAISRFNHFINDSLLEGAIDALKRIGQVSDENITVVWVPGAYELPLTVRALASSGRYDAVVALG
TVIRGGTAHFEFVAGECSSGLASVALNTDVPVAFGVLTTETIEQAIDRAGAKAGNKGAEAALTALEMINVLKAIKA

Sequences:

>Translated_156_residues
MKTIQGVVAAPQARVAIAISRFNHFINDSLLEGAIDALKRIGQVSDENITVVWVPGAYELPLTVRALASSGRYDAVVALG
TVIRGGTAHFEFVAGECSSGLASVALNTDVPVAFGVLTTETIEQAIDRAGAKAGNKGAEAALTALEMINVLKAIKA
>Mature_156_residues
MKTIQGVVAAPQARVAIAISRFNHFINDSLLEGAIDALKRIGQVSDENITVVWVPGAYELPLTVRALASSGRYDAVVALG
TVIRGGTAHFEFVAGECSSGLASVALNTDVPVAFGVLTTETIEQAIDRAGAKAGNKGAEAALTALEMINVLKAIKA

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=156, Percent_Identity=80.1282051282051, Blast_Score=252, Evalue=8e-69,
Organism=Saccharomyces cerevisiae, GI6324429, Length=150, Percent_Identity=36, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_EDWI9 (C5BCH5)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002932504.1
- GeneID:   7959440
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1057
- OMA:   KAGNKGW
- ProtClustDB:   CLSK2812935
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16147; Mature: 16147

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIQGVVAAPQARVAIAISRFNHFINDSLLEGAIDALKRIGQVSDENITVVWVPGAYEL
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
PLTVRALASSGRYDAVVALGTVIRGGTAHFEFVAGECSSGLASVALNTDVPVAFGVLTTE
CHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHH
TIEQAIDRAGAKAGNKGAEAALTALEMINVLKAIKA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTIQGVVAAPQARVAIAISRFNHFINDSLLEGAIDALKRIGQVSDENITVVWVPGAYEL
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
PLTVRALASSGRYDAVVALGTVIRGGTAHFEFVAGECSSGLASVALNTDVPVAFGVLTTE
CHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHH
TIEQAIDRAGAKAGNKGAEAALTALEMINVLKAIKA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA