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The map label for this gene is mnmE [H]

Identifier: 238917975

GI number: 238917975

Start: 2139144

End: 2140523

Strand: Reverse

Name: mnmE [H]

Synonym: EUBELI_02065

Alternate gene names: 238917975

Gene position: 2140523-2139144 (Counterclockwise)

Preceding gene: 238917976

Following gene: 238917974

Centisome position: 99.83

GC content: 34.71

Gene sequence:

>1380_bases
ATGGAAATGGAGGATACAATAGCAGCTATATCTACAAGTACAGTGTCAAGCGGTGGAATTAGTATTGTAAGAATAAGCGG
TCCTGATGCAATAATTATTGGAGATAAAGTTTTTAAGTCAAAAAAGAATATTAAGCTTGCAAATGTGCAGAGTCATACAG
TACATTTTGGAAATATATGTGATAACGGGAATGAAATTGATGAAGCACTTGTGATTGTAATGAAATCTCCTAACACATAT
ACAAAAGAGAATATTGTTGAGATTGACTGTCATGGAGGTATTCTTGTTACTAAGAAGGTGCTGGAGGCGGTACTTAACGC
TGGAGCAAGGCTTGCAGAGCCTGGTGAATTTACAAAGAGGGCATTTCTTAATGGAAGAATTGACCTGTCACAGGCGGAGG
CTGTTATTGATATTATTAATGCAAAGAATGACTACGCATTGAAATCATCTGTTAATCAGTTAGATGGTAAGTTATCTGAA
AAGATTAAGAACATAAGAGAGATTATATTAAATAATGTTGCATTTATTGAAGCTGCACTTGATGATCCTGAACATTTTGA
TATAGATGCTAATGTAAATAACCTTAAAAATGATGTGGAAAACTGTCTTAATAATGTGGATAACTTGTTAAAAACATGCG
ACAATGGCAGAATTATGCATGATGGCGTGCGTACCGTCATTTTGGGGAAAACTAATGCAGGAAAGTCAAGCCTGCTTAAT
GTACTTGCAAGAGAAGAGAGAGCAATAGTTACTGATATAGAAGGAACTACAAGAGATACTCTTGAGGAAATGATTAATCT
GTCGGGAGTTACTCTTAATCTTATTGATACTGCAGGAATCAGAAAAACTGATGATGTTGTTGAAAGCATTGGTGTTAATA
AGGCTAAGAAGCTTGCAGAAGTTGCAGACCTTGTAATATATGTGGTTGATTCTTCAAGGTCACTTGATGATAATGACTAC
GAGATTATGGAACTTATCAAGGATAAGAAGGTTCTTGTAATACTTAATAAAGCTGATCTTGCACAGGTTACAACAGCAGA
AGATATTAAGAAAATAATCAATTGTGAGACAGTTACTATATCTGCAAAGCAGGAGACAGGAATTGAAGAACTTGAGGAAA
CTGTTAAGAATATGTTCTTTAACGGTGAAGTTAAGTTTAATGAAGATGTATATATTACAAGTACAAGACATAAAGAACTT
TTAAAGAAGGCTGAGAACAGCTTAAAGCTTGTACTTGATGGAATTGATAACATGGTAAGTGAAGATTTCCTTACTATTGA
TTTAATGGCTGCATATGAGAATCTTGGTCTTATAATAGGAGAGGAGATTGAAGATGACCTCGCCAACAGAATTTTCTCAA
AGTTCTGTATGGGTAAGTAA

Upstream 100 bases:

>100_bases
TATCTTGAAAGAGAGAATAATAACAGATATAACAGATAATTAGATATATGATTTTATTAAATGCTGTGGTATTATTATCA
CAGCATTTTTTATATGGAGG

Downstream 100 bases:

>100_bases
TGCAGGTTGATAAGGAGAAGTAATATGCCTAATATTAGAGAAGAATATGATGTAGTTGTAGTAGGTGCTGGTCACGCAGG
TTGTGAGGCTGCCCTTGCTG

Product: tRNA modification GTPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MEMEDTIAAISTSTVSSGGISIVRISGPDAIIIGDKVFKSKKNIKLANVQSHTVHFGNICDNGNEIDEALVIVMKSPNTY
TKENIVEIDCHGGILVTKKVLEAVLNAGARLAEPGEFTKRAFLNGRIDLSQAEAVIDIINAKNDYALKSSVNQLDGKLSE
KIKNIREIILNNVAFIEAALDDPEHFDIDANVNNLKNDVENCLNNVDNLLKTCDNGRIMHDGVRTVILGKTNAGKSSLLN
VLAREERAIVTDIEGTTRDTLEEMINLSGVTLNLIDTAGIRKTDDVVESIGVNKAKKLAEVADLVIYVVDSSRSLDDNDY
EIMELIKDKKVLVILNKADLAQVTTAEDIKKIINCETVTISAKQETGIEELEETVKNMFFNGEVKFNEDVYITSTRHKEL
LKKAENSLKLVLDGIDNMVSEDFLTIDLMAAYENLGLIIGEEIEDDLANRIFSKFCMGK

Sequences:

>Translated_459_residues
MEMEDTIAAISTSTVSSGGISIVRISGPDAIIIGDKVFKSKKNIKLANVQSHTVHFGNICDNGNEIDEALVIVMKSPNTY
TKENIVEIDCHGGILVTKKVLEAVLNAGARLAEPGEFTKRAFLNGRIDLSQAEAVIDIINAKNDYALKSSVNQLDGKLSE
KIKNIREIILNNVAFIEAALDDPEHFDIDANVNNLKNDVENCLNNVDNLLKTCDNGRIMHDGVRTVILGKTNAGKSSLLN
VLAREERAIVTDIEGTTRDTLEEMINLSGVTLNLIDTAGIRKTDDVVESIGVNKAKKLAEVADLVIYVVDSSRSLDDNDY
EIMELIKDKKVLVILNKADLAQVTTAEDIKKIINCETVTISAKQETGIEELEETVKNMFFNGEVKFNEDVYITSTRHKEL
LKKAENSLKLVLDGIDNMVSEDFLTIDLMAAYENLGLIIGEEIEDDLANRIFSKFCMGK
>Mature_459_residues
MEMEDTIAAISTSTVSSGGISIVRISGPDAIIIGDKVFKSKKNIKLANVQSHTVHFGNICDNGNEIDEALVIVMKSPNTY
TKENIVEIDCHGGILVTKKVLEAVLNAGARLAEPGEFTKRAFLNGRIDLSQAEAVIDIINAKNDYALKSSVNQLDGKLSE
KIKNIREIILNNVAFIEAALDDPEHFDIDANVNNLKNDVENCLNNVDNLLKTCDNGRIMHDGVRTVILGKTNAGKSSLLN
VLAREERAIVTDIEGTTRDTLEEMINLSGVTLNLIDTAGIRKTDDVVESIGVNKAKKLAEVADLVIYVVDSSRSLDDNDY
EIMELIKDKKVLVILNKADLAQVTTAEDIKKIINCETVTISAKQETGIEELEETVKNMFFNGEVKFNEDVYITSTRHKEL
LKKAENSLKLVLDGIDNMVSEDFLTIDLMAAYENLGLIIGEEIEDDLANRIFSKFCMGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=28.7234042553192, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI193082993, Length=459, Percent_Identity=29.1938997821351, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI193082988, Length=501, Percent_Identity=26.3473053892216, Blast_Score=173, Evalue=3e-43,
Organism=Escherichia coli, GI2367268, Length=465, Percent_Identity=34.1935483870968, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI87082120, Length=170, Percent_Identity=30.5882352941176, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=466, Percent_Identity=28.9699570815451, Blast_Score=179, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=502, Percent_Identity=29.4820717131474, Blast_Score=195, Evalue=1e-50,
Organism=Drosophila melanogaster, GI45550813, Length=481, Percent_Identity=28.2744282744283, Blast_Score=179, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50505; Mature: 50505

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMEDTIAAISTSTVSSGGISIVRISGPDAIIIGDKVFKSKKNIKLANVQSHTVHFGNIC
CCCHHHHHHHHHCCCCCCCEEEEEECCCCEEEECHHHHHCCCCEEEEECCCCEEEECCCC
DNGNEIDEALVIVMKSPNTYTKENIVEIDCHGGILVTKKVLEAVLNAGARLAEPGEFTKR
CCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCEEHHHHHHHHHHCCCCCCCCCCHHHHH
AFLNGRIDLSQAEAVIDIINAKNDYALKSSVNQLDGKLSEKIKNIREIILNNVAFIEAAL
HHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEC
DDPEHFDIDANVNNLKNDVENCLNNVDNLLKTCDNGRIMHDGVRTVILGKTNAGKSSLLN
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEECCCCCHHHHHH
VLAREERAIVTDIEGTTRDTLEEMINLSGVTLNLIDTAGIRKTDDVVESIGVNKAKKLAE
HHHHCCCEEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHH
VADLVIYVVDSSRSLDDNDYEIMELIKDKKVLVILNKADLAQVTTAEDIKKIINCETVTI
HHHEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHCCHHHHHHHHCCCEEEE
SAKQETGIEELEETVKNMFFNGEVKFNEDVYITSTRHKELLKKAENSLKLVLDGIDNMVS
ECHHHCCHHHHHHHHHHHEECCEEEECCCEEEECHHHHHHHHHHHHHHEEHHHHHHHHHH
EDFLTIDLMAAYENLGLIIGEEIEDDLANRIFSKFCMGK
CCHHEEHHHHHHHHCCEEECCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEMEDTIAAISTSTVSSGGISIVRISGPDAIIIGDKVFKSKKNIKLANVQSHTVHFGNIC
CCCHHHHHHHHHCCCCCCCEEEEEECCCCEEEECHHHHHCCCCEEEEECCCCEEEECCCC
DNGNEIDEALVIVMKSPNTYTKENIVEIDCHGGILVTKKVLEAVLNAGARLAEPGEFTKR
CCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCEEHHHHHHHHHHCCCCCCCCCCHHHHH
AFLNGRIDLSQAEAVIDIINAKNDYALKSSVNQLDGKLSEKIKNIREIILNNVAFIEAAL
HHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEC
DDPEHFDIDANVNNLKNDVENCLNNVDNLLKTCDNGRIMHDGVRTVILGKTNAGKSSLLN
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEECCCCCHHHHHH
VLAREERAIVTDIEGTTRDTLEEMINLSGVTLNLIDTAGIRKTDDVVESIGVNKAKKLAE
HHHHCCCEEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHH
VADLVIYVVDSSRSLDDNDYEIMELIKDKKVLVILNKADLAQVTTAEDIKKIINCETVTI
HHHEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHCCHHHHHHHHCCCEEEE
SAKQETGIEELEETVKNMFFNGEVKFNEDVYITSTRHKELLKKAENSLKLVLDGIDNMVS
ECHHHCCHHHHHHHHHHHEECCEEEECCCEEEECHHHHHHHHHHHHHHEEHHHHHHHHHH
EDFLTIDLMAAYENLGLIIGEEIEDDLANRIFSKFCMGK
CCHHEEHHHHHHHHCCEEECCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA