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The map label for this gene is mnmG [H]

Identifier: 238917974

GI number: 238917974

Start: 2137236

End: 2139119

Strand: Reverse

Name: mnmG [H]

Synonym: EUBELI_02064

Alternate gene names: 238917974

Gene position: 2139119-2137236 (Counterclockwise)

Preceding gene: 238917975

Following gene: 238917973

Centisome position: 99.76

GC content: 39.54

Gene sequence:

>1884_bases
ATGCCTAATATTAGAGAAGAATATGATGTAGTTGTAGTAGGTGCTGGTCACGCAGGTTGTGAGGCTGCCCTTGCTGCTGC
AAGAATGGGACTTGAAACAATTATATTTACAGTAAGCGTTGAAAGTATAGCAATGATGCCTTGTAATCCTAATATAGGAG
GAAGTTCTAAGGGACATCTTGTAAGAGAAATAGATGCTCTTGGTGGAGAGATGGGAAAGAACATTGATGCTACATTTATA
CAGTCAAAGATGTTAAATGCATCAAAAGGCCCTGCGGTTCATTCGCTCCGCGCACAGGCTGACAAGTCTGATTATAGCAG
AAGAATGAGAAATGTTCTTGAGAATACTGAACATCTGCATATAAGACAGGCAGAGGTTTCAGAGATTATAGTTAATGATG
GAAAAATTGGCGGTGTAAAGACTGTTTCTGGTGCGGAATACATAGCTAAGGCAGTTGTTTTATGTACAGGAGTATATCTT
AAGGCAAGATGTATATATGGTGATGTAAGCTATCATACAGGTCCTAATGGATTACAGGCTGCCAATCATCTTACACAGTC
ACTTATTGATAATGGAATTGAAGTGAGAAGATTCAAGACGGGTACACCTGCCAGAGTTGATAAAAGGTCAGTGGATTTTT
CTAAGATGACAGAGCAGTTCGGTGATAAGAGAGTTGTGCCATTTTCATTTGAAACAGACCCTGAGAGCATACAGAAAGAT
CAGGTAAGCTGCTGGCTTACATATACTAATGAAGAGACACACAAGATTATTATGGATAATCTTGACCGTTCACCTCTTTA
TTCAGGAGATATCCACGGAACAGGCCCAAGATATTGTCCATCAATTGAGGATAAGGTTGTAAGATTTGCAGACAAAGACA
GGCATCAGGTATTCATTGAGCCAGAGGGCTTGTATACTAATGAAATGTATCTTGGTGGAATGAGCAGCTCAATGCCTGAG
GATGTGCAGTATGCAATGTATAGAACAGTTCCTGGTCTTGAAAATGTCCGAATCGTAAGAAATGCTTATGCTATTGAGTA
TGACTGTATTAACGCTGTTCAGTTAAAGTCTTCTCTTGAATTCAAGAAGATTGAAGGATTATTTGCTGGTGGACAGTTTA
ACGGAAGCTCAGGATATGAGGAAGCCGCTGCACAGGGAATTATTGCTGGTATCAATGCGGCACTTTTCGTTAAGAAGGAA
GAACCACTTATACTTGACCGTTCAGAAAGTTATATAGGTGTTCTTATAGATGACCTTGTAACTAAGGAGACAGCAGAGCC
TTACAGAATGATGACAAGCCGTGCAGAGTACAGACTTATATTAAGACAGGATAATGCAGATATAAGACTTACTAAATATG
GTTACAGAGTCGGACTTATAAGCGAAGAAAGAATGAACCGTCTTACCCGCAAGATTCAGCTTATAGATGAAGAAATTGAG
CGTGTTAAGTCAGTTAATATAGGAACTGGAAGCGAAGTACAGAAGGTACTTACAGATAACGGAAGTACAGAGCTTACAAC
AGGTGCTACACTTGCAGAACTTATAAGAAGACCGGAGCTTTCATATGATGTTCTTGCTCCAATTGATAAGCACAGACCAG
AGCTGCCTTGGGATGTATGTGAGCAGGTTAATATTAATCTGAAATACGAGGGATATATAGACAGACAGTTAAGACAGGTT
GAGCATTTTAAAAAACTTGAAAATAAGCTTATACCAGACAGCCTTGATTACTTAGGAATAAGCGGATTAAGAAAAGAGGC
TATGCAGAAGCTTGATAAATTCAGACCTCGTTCAATTGGACAGGCTTCAAGAATATCGGGTGTTTCGCCTGCTGATATAT
CAGTGCTTCTTGTTTACCTGGAGTCTCTTAGAAGAAAGTATTAA

Upstream 100 bases:

>100_bases
TGGTCTTATAATAGGAGAGGAGATTGAAGATGACCTCGCCAACAGAATTTTCTCAAAGTTCTGTATGGGTAAGTAATGCA
GGTTGATAAGGAGAAGTAAT

Downstream 100 bases:

>100_bases
TCATTGATAAGGAGAAATGTATGATAAATGAAACATTTAAGAATAATCTGAATTCTATAGGTGTTGAGCTTACAGACAGA
CAGTACGAGCAGTTTGATAT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 627; Mature: 626

Protein sequence:

>627_residues
MPNIREEYDVVVVGAGHAGCEAALAAARMGLETIIFTVSVESIAMMPCNPNIGGSSKGHLVREIDALGGEMGKNIDATFI
QSKMLNASKGPAVHSLRAQADKSDYSRRMRNVLENTEHLHIRQAEVSEIIVNDGKIGGVKTVSGAEYIAKAVVLCTGVYL
KARCIYGDVSYHTGPNGLQAANHLTQSLIDNGIEVRRFKTGTPARVDKRSVDFSKMTEQFGDKRVVPFSFETDPESIQKD
QVSCWLTYTNEETHKIIMDNLDRSPLYSGDIHGTGPRYCPSIEDKVVRFADKDRHQVFIEPEGLYTNEMYLGGMSSSMPE
DVQYAMYRTVPGLENVRIVRNAYAIEYDCINAVQLKSSLEFKKIEGLFAGGQFNGSSGYEEAAAQGIIAGINAALFVKKE
EPLILDRSESYIGVLIDDLVTKETAEPYRMMTSRAEYRLILRQDNADIRLTKYGYRVGLISEERMNRLTRKIQLIDEEIE
RVKSVNIGTGSEVQKVLTDNGSTELTTGATLAELIRRPELSYDVLAPIDKHRPELPWDVCEQVNINLKYEGYIDRQLRQV
EHFKKLENKLIPDSLDYLGISGLRKEAMQKLDKFRPRSIGQASRISGVSPADISVLLVYLESLRRKY

Sequences:

>Translated_627_residues
MPNIREEYDVVVVGAGHAGCEAALAAARMGLETIIFTVSVESIAMMPCNPNIGGSSKGHLVREIDALGGEMGKNIDATFI
QSKMLNASKGPAVHSLRAQADKSDYSRRMRNVLENTEHLHIRQAEVSEIIVNDGKIGGVKTVSGAEYIAKAVVLCTGVYL
KARCIYGDVSYHTGPNGLQAANHLTQSLIDNGIEVRRFKTGTPARVDKRSVDFSKMTEQFGDKRVVPFSFETDPESIQKD
QVSCWLTYTNEETHKIIMDNLDRSPLYSGDIHGTGPRYCPSIEDKVVRFADKDRHQVFIEPEGLYTNEMYLGGMSSSMPE
DVQYAMYRTVPGLENVRIVRNAYAIEYDCINAVQLKSSLEFKKIEGLFAGGQFNGSSGYEEAAAQGIIAGINAALFVKKE
EPLILDRSESYIGVLIDDLVTKETAEPYRMMTSRAEYRLILRQDNADIRLTKYGYRVGLISEERMNRLTRKIQLIDEEIE
RVKSVNIGTGSEVQKVLTDNGSTELTTGATLAELIRRPELSYDVLAPIDKHRPELPWDVCEQVNINLKYEGYIDRQLRQV
EHFKKLENKLIPDSLDYLGISGLRKEAMQKLDKFRPRSIGQASRISGVSPADISVLLVYLESLRRKY
>Mature_626_residues
PNIREEYDVVVVGAGHAGCEAALAAARMGLETIIFTVSVESIAMMPCNPNIGGSSKGHLVREIDALGGEMGKNIDATFIQ
SKMLNASKGPAVHSLRAQADKSDYSRRMRNVLENTEHLHIRQAEVSEIIVNDGKIGGVKTVSGAEYIAKAVVLCTGVYLK
ARCIYGDVSYHTGPNGLQAANHLTQSLIDNGIEVRRFKTGTPARVDKRSVDFSKMTEQFGDKRVVPFSFETDPESIQKDQ
VSCWLTYTNEETHKIIMDNLDRSPLYSGDIHGTGPRYCPSIEDKVVRFADKDRHQVFIEPEGLYTNEMYLGGMSSSMPED
VQYAMYRTVPGLENVRIVRNAYAIEYDCINAVQLKSSLEFKKIEGLFAGGQFNGSSGYEEAAAQGIIAGINAALFVKKEE
PLILDRSESYIGVLIDDLVTKETAEPYRMMTSRAEYRLILRQDNADIRLTKYGYRVGLISEERMNRLTRKIQLIDEEIER
VKSVNIGTGSEVQKVLTDNGSTELTTGATLAELIRRPELSYDVLAPIDKHRPELPWDVCEQVNINLKYEGYIDRQLRQVE
HFKKLENKLIPDSLDYLGISGLRKEAMQKLDKFRPRSIGQASRISGVSPADISVLLVYLESLRRKY

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=638, Percent_Identity=38.871473354232, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI19882217, Length=662, Percent_Identity=37.7643504531722, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI183227703, Length=678, Percent_Identity=36.5781710914454, Blast_Score=404, Evalue=1e-112,
Organism=Escherichia coli, GI2367273, Length=624, Percent_Identity=48.8782051282051, Blast_Score=590, Evalue=1e-169,
Organism=Caenorhabditis elegans, GI17534255, Length=619, Percent_Identity=39.2568659127625, Blast_Score=439, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6321202, Length=619, Percent_Identity=42.1647819063005, Blast_Score=463, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24658174, Length=629, Percent_Identity=40.3815580286169, Blast_Score=405, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 70090; Mature: 69959

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS01280 GIDA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNIREEYDVVVVGAGHAGCEAALAAARMGLETIIFTVSVESIAMMPCNPNIGGSSKGHL
CCCCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEEEEECCEEEECCCCCCCCCCCCCH
VREIDALGGEMGKNIDATFIQSKMLNASKGPAVHSLRAQADKSDYSRRMRNVLENTEHLH
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCEEE
IRQAEVSEIIVNDGKIGGVKTVSGAEYIAKAVVLCTGVYLKARCIYGDVSYHTGPNGLQA
EEHHHHHHHEECCCCCCCEEECCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCHHHH
ANHLTQSLIDNGIEVRRFKTGTPARVDKRSVDFSKMTEQFGDKRVVPFSFETDPESIQKD
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCHHHHCCC
QVSCWLTYTNEETHKIIMDNLDRSPLYSGDIHGTGPRYCPSIEDKVVRFADKDRHQVFIE
CEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEC
PEGLYTNEMYLGGMSSSMPEDVQYAMYRTVPGLENVRIVRNAYAIEYDCINAVQLKSSLE
CCCCEECCEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEHEEEEEECCCCHHHHHHCCC
FKKIEGLFAGGQFNGSSGYEEAAAQGIIAGINAALFVKKEEPLILDRSESYIGVLIDDLV
HHHHCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCEEHHHHHHHH
TKETAEPYRMMTSRAEYRLILRQDNADIRLTKYGYRVGLISEERMNRLTRKIQLIDEEIE
HHCCCCHHHHHHCCCCEEEEEEECCCCEEEEEECEEEEEECHHHHHHHHHHHHHHHHHHH
RVKSVNIGTGSEVQKVLTDNGSTELTTGATLAELIRRPELSYDVLAPIDKHRPELPWDVC
HHHCCCCCCCHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHH
EQVNINLKYEGYIDRQLRQVEHFKKLENKLIPDSLDYLGISGLRKEAMQKLDKFRPRSIG
HHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHCCCCCCC
QASRISGVSPADISVLLVYLESLRRKY
CHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PNIREEYDVVVVGAGHAGCEAALAAARMGLETIIFTVSVESIAMMPCNPNIGGSSKGHL
CCCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEEEEECCEEEECCCCCCCCCCCCCH
VREIDALGGEMGKNIDATFIQSKMLNASKGPAVHSLRAQADKSDYSRRMRNVLENTEHLH
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCEEE
IRQAEVSEIIVNDGKIGGVKTVSGAEYIAKAVVLCTGVYLKARCIYGDVSYHTGPNGLQA
EEHHHHHHHEECCCCCCCEEECCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCHHHH
ANHLTQSLIDNGIEVRRFKTGTPARVDKRSVDFSKMTEQFGDKRVVPFSFETDPESIQKD
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCHHHHCCC
QVSCWLTYTNEETHKIIMDNLDRSPLYSGDIHGTGPRYCPSIEDKVVRFADKDRHQVFIE
CEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEC
PEGLYTNEMYLGGMSSSMPEDVQYAMYRTVPGLENVRIVRNAYAIEYDCINAVQLKSSLE
CCCCEECCEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEHEEEEEECCCCHHHHHHCCC
FKKIEGLFAGGQFNGSSGYEEAAAQGIIAGINAALFVKKEEPLILDRSESYIGVLIDDLV
HHHHCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCEEHHHHHHHH
TKETAEPYRMMTSRAEYRLILRQDNADIRLTKYGYRVGLISEERMNRLTRKIQLIDEEIE
HHCCCCHHHHHHCCCCEEEEEEECCCCEEEEEECEEEEEECHHHHHHHHHHHHHHHHHHH
RVKSVNIGTGSEVQKVLTDNGSTELTTGATLAELIRRPELSYDVLAPIDKHRPELPWDVC
HHHCCCCCCCHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHH
EQVNINLKYEGYIDRQLRQVEHFKKLENKLIPDSLDYLGISGLRKEAMQKLDKFRPRSIG
HHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHCCCCCCC
QASRISGVSPADISVLLVYLESLRRKY
CHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA