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The map label for this gene is cwlD [H]

Identifier: 238917861

GI number: 238917861

Start: 2008736

End: 2009524

Strand: Reverse

Name: cwlD [H]

Synonym: EUBELI_01942

Alternate gene names: 238917861

Gene position: 2009524-2008736 (Counterclockwise)

Preceding gene: 238917862

Following gene: 238917860

Centisome position: 93.72

GC content: 38.78

Gene sequence:

>789_bases
ATGCCAGAAAAAGCTGATATAAGCAGGACACTTCTTCTGCCGTTTATGTCAATAATACTTCTTGTTGCTGTTTACATGAC
AGCCAATGTACTTCTTCCATTAAAGGCCAATGGCAAAGGCAGCAAAGAAAGTGAAGATGTGCAGGAGAGACAACATTTGT
CAGATGATGCAGGTGATGGTGAAGTCGTTGAGATGAATATTAATTTTTCTGAAAAATGTATTGTTATTGATAGTGGGCAT
GGTGGCGCAGACCCAGGTAAAGTAGGGGTTGCGGGAACAAATGAAAAAGAGATTAATCTTGCAATTGCAAAAAAATTACA
GGAACGGCTTGAAGATGCCCAGATTAATGTTATTATGACAAGGGACACGGATGATGATTTAAGTGTGGAGTCCGATAAGA
GTAAAAAGAAAGCTGACCTGGAAAGAAGATGTGATATAATCAATAGCAGTGGTGCTGATATGGTAATCAGTATCCATCAG
AACAGTTATGTCACACCTAAGGCAGAAGGTGCACAGGTATTTTATTATAAGAAGTCTGAGGAAAGTAAAAGAATAGCGCA
GATAATGCAGGGGATTCTTGGTGAGAAGTTAGGTACAACAAGGCAGATTAAGTCAGATGTTAATTATTACATACTGCTTC
ATTCCAAGCTGCCGACAATTATATCAGAGTGTGGTTTCTTGTCTAATCCGGAAGAGGAGCAGAGACTGTGTACGGAAGAA
TATCAGGAAAAAGTGGCTGATGCTCTGTATTGTGGTATAATAGAATATCTGATGATGAATATGAAGTAA

Upstream 100 bases:

>100_bases
TCATCAGTATGGTCAGTTATCAGCAAATATAATAAATAAAAGTAATATGCATAAACCTTCTGTAATAAATTAGTAGAGAA
TTACAGGTTTCGGAGTCGTT

Downstream 100 bases:

>100_bases
TGAAAGCTGGCGAAAGGTTTTATGGAAACAATAATAAAGAAGATAACACACACAGAAGAAGATAAGAAAATATATGAACA
GGCAGGACAGATTCTAAGAG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MPEKADISRTLLLPFMSIILLVAVYMTANVLLPLKANGKGSKESEDVQERQHLSDDAGDGEVVEMNINFSEKCIVIDSGH
GGADPGKVGVAGTNEKEINLAIAKKLQERLEDAQINVIMTRDTDDDLSVESDKSKKKADLERRCDIINSSGADMVISIHQ
NSYVTPKAEGAQVFYYKKSEESKRIAQIMQGILGEKLGTTRQIKSDVNYYILLHSKLPTIISECGFLSNPEEEQRLCTEE
YQEKVADALYCGIIEYLMMNMK

Sequences:

>Translated_262_residues
MPEKADISRTLLLPFMSIILLVAVYMTANVLLPLKANGKGSKESEDVQERQHLSDDAGDGEVVEMNINFSEKCIVIDSGH
GGADPGKVGVAGTNEKEINLAIAKKLQERLEDAQINVIMTRDTDDDLSVESDKSKKKADLERRCDIINSSGADMVISIHQ
NSYVTPKAEGAQVFYYKKSEESKRIAQIMQGILGEKLGTTRQIKSDVNYYILLHSKLPTIISECGFLSNPEEEQRLCTEE
YQEKVADALYCGIIEYLMMNMK
>Mature_261_residues
PEKADISRTLLLPFMSIILLVAVYMTANVLLPLKANGKGSKESEDVQERQHLSDDAGDGEVVEMNINFSEKCIVIDSGHG
GADPGKVGVAGTNEKEINLAIAKKLQERLEDAQINVIMTRDTDDDLSVESDKSKKKADLERRCDIINSSGADMVISIHQN
SYVTPKAEGAQVFYYKKSEESKRIAQIMQGILGEKLGTTRQIKSDVNYYILLHSKLPTIISECGFLSNPEEEQRLCTEEY
QEKVADALYCGIIEYLMMNMK

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=233, Percent_Identity=28.755364806867, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790611, Length=242, Percent_Identity=26.0330578512397, Blast_Score=95, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR014234 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 29158; Mature: 29026

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEKADISRTLLLPFMSIILLVAVYMTANVLLPLKANGKGSKESEDVQERQHLSDDAGDG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCC
EVVEMNINFSEKCIVIDSGHGGADPGKVGVAGTNEKEINLAIAKKLQERLEDAQINVIMT
CEEEEEECCCCEEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEE
RDTDDDLSVESDKSKKKADLERRCDIINSSGADMVISIHQNSYVTPKAEGAQVFYYKKSE
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCC
ESKRIAQIMQGILGEKLGTTRQIKSDVNYYILLHSKLPTIISECGFLSNPEEEQRLCTEE
HHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHH
YQEKVADALYCGIIEYLMMNMK
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PEKADISRTLLLPFMSIILLVAVYMTANVLLPLKANGKGSKESEDVQERQHLSDDAGDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCC
EVVEMNINFSEKCIVIDSGHGGADPGKVGVAGTNEKEINLAIAKKLQERLEDAQINVIMT
CEEEEEECCCCEEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEE
RDTDDDLSVESDKSKKKADLERRCDIINSSGADMVISIHQNSYVTPKAEGAQVFYYKKSE
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCC
ESKRIAQIMQGILGEKLGTTRQIKSDVNYYILLHSKLPTIISECGFLSNPEEEQRLCTEE
HHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHH
YQEKVADALYCGIIEYLMMNMK
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559346; 8969501; 9384377 [H]