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The map label for this gene is cysD2 [H]

Identifier: 238917813

GI number: 238917813

Start: 1958777

End: 1959676

Strand: Reverse

Name: cysD2 [H]

Synonym: EUBELI_01894

Alternate gene names: 238917813

Gene position: 1959676-1958777 (Counterclockwise)

Preceding gene: 238917814

Following gene: 238917812

Centisome position: 91.39

GC content: 43.11

Gene sequence:

>900_bases
ATGGGTAATTTATCACATTTGGACGCACTCGAAGCAGAGGCGATATATATTATAAGGGAAGTTGCAGCAGAATGCGAAAA
GCCGGTAATGCTGTATTCTATAGGAAAGGACAGCTCAGTTATGCTTCATCTTGCAATGAAAGCATTCTATCCTGAGAAAC
CACCTTTTCCATTTCTTCACATTGACACAACATGGAAGTTCAAGGAAATGATTAAGTTCAGGGATGAGACAGCGAAAAAG
CTTGGAATCGAGATGCTTACATACACTAACGAAGAGGGTGTAAAGCAGGGAATCAACCCATTTGACCACGGTTCAGCTTA
CACTGATATCATGAAGACTCAGGCTCTTAAGCAGGCACTTAACAAATACGGCTTTACAGCAGCATTTGGCGGAGGACGAA
GAGACGAGGAGAAATCAAGAGCTAAGGAGAGAATTTTCTCTTTCAGAAATACAGCACAGGCATGGGACCCTAAGAACCAG
CGTCCGGAAATGTGGAAGCTTTACAACACTAAGATTCACAAGGGCGAGAGCATGAGAGTTTTCCCTATCTCTAACTGGAC
TGAGAAGGATATCTGGCAGTACATCCAGAGAGATAAGATTGATATTGTACCTCTTTATTTTGCAGCAGAAAGACCTGTTG
TATACCGTGACGGCAATATCATCATGGTTGATGATGACAGAATGAGACTTCTTCCAGGCGAAAAGCCACAGATGAAGAAG
GTAAGATTCAGAACTCTTGGCTGTTATCCACTTACAGGCGGTGTTGAGTCAGACGCAGAAACTCTTGACGAGATTATCGA
AGAGACTTTAAGTGCGGTTGAATCAGAGAGAACAAGCCGTGTTATAGACCATGAGGAAGCAGGAAGCATGGAAAGAAGAA
AGAAAGAGGGGTACTTCTAG

Upstream 100 bases:

>100_bases
AGGGAAGTATCAATACATGGACGGTGACTTCGCCGGACGGAACAGTTAAGACGCTTGAGGTCAACAGACAGGAATCTAAT
AAATATTAGGGAGGAATATC

Downstream 100 bases:

>100_bases
AATGAAAGGTTTACTTAAATTTATTACATGCGGTAGTGTTGATGATGGAAAATCTACACTTATCGGACACATTTTATATG
ATTCAAAGTTATTGTATGCT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit 2; Sulfate adenylate transferase 2; SAT 2 [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MGNLSHLDALEAEAIYIIREVAAECEKPVMLYSIGKDSSVMLHLAMKAFYPEKPPFPFLHIDTTWKFKEMIKFRDETAKK
LGIEMLTYTNEEGVKQGINPFDHGSAYTDIMKTQALKQALNKYGFTAAFGGGRRDEEKSRAKERIFSFRNTAQAWDPKNQ
RPEMWKLYNTKIHKGESMRVFPISNWTEKDIWQYIQRDKIDIVPLYFAAERPVVYRDGNIIMVDDDRMRLLPGEKPQMKK
VRFRTLGCYPLTGGVESDAETLDEIIEETLSAVESERTSRVIDHEEAGSMERRKKEGYF

Sequences:

>Translated_299_residues
MGNLSHLDALEAEAIYIIREVAAECEKPVMLYSIGKDSSVMLHLAMKAFYPEKPPFPFLHIDTTWKFKEMIKFRDETAKK
LGIEMLTYTNEEGVKQGINPFDHGSAYTDIMKTQALKQALNKYGFTAAFGGGRRDEEKSRAKERIFSFRNTAQAWDPKNQ
RPEMWKLYNTKIHKGESMRVFPISNWTEKDIWQYIQRDKIDIVPLYFAAERPVVYRDGNIIMVDDDRMRLLPGEKPQMKK
VRFRTLGCYPLTGGVESDAETLDEIIEETLSAVESERTSRVIDHEEAGSMERRKKEGYF
>Mature_298_residues
GNLSHLDALEAEAIYIIREVAAECEKPVMLYSIGKDSSVMLHLAMKAFYPEKPPFPFLHIDTTWKFKEMIKFRDETAKKL
GIEMLTYTNEEGVKQGINPFDHGSAYTDIMKTQALKQALNKYGFTAAFGGGRRDEEKSRAKERIFSFRNTAQAWDPKNQR
PEMWKLYNTKIHKGESMRVFPISNWTEKDIWQYIQRDKIDIVPLYFAAERPVVYRDGNIIMVDDDRMRLLPGEKPQMKKV
RFRTLGCYPLTGGVESDAETLDEIIEETLSAVESERTSRVIDHEEAGSMERRKKEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=69.3602693602694, Blast_Score=430, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34687; Mature: 34556

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNLSHLDALEAEAIYIIREVAAECEKPVMLYSIGKDSSVMLHLAMKAFYPEKPPFPFLH
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEE
IDTTWKFKEMIKFRDETAKKLGIEMLTYTNEEGVKQGINPFDHGSAYTDIMKTQALKQAL
ECCCHHHHHHHHHHHHHHHHHCCHHEEECCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
NKYGFTAAFGGGRRDEEKSRAKERIFSFRNTAQAWDPKNQRPEMWKLYNTKIHKGESMRV
HHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCEECCCCCEEE
FPISNWTEKDIWQYIQRDKIDIVPLYFAAERPVVYRDGNIIMVDDDRMRLLPGEKPQMKK
EECCCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCEEEECCCCEEECCCCCCHHHH
VRFRTLGCYPLTGGVESDAETLDEIIEETLSAVESERTSRVIDHEEAGSMERRKKEGYF
HHHHEECCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHCCCC
>Mature Secondary Structure 
GNLSHLDALEAEAIYIIREVAAECEKPVMLYSIGKDSSVMLHLAMKAFYPEKPPFPFLH
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEE
IDTTWKFKEMIKFRDETAKKLGIEMLTYTNEEGVKQGINPFDHGSAYTDIMKTQALKQAL
ECCCHHHHHHHHHHHHHHHHHCCHHEEECCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
NKYGFTAAFGGGRRDEEKSRAKERIFSFRNTAQAWDPKNQRPEMWKLYNTKIHKGESMRV
HHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCEECCCCCEEE
FPISNWTEKDIWQYIQRDKIDIVPLYFAAERPVVYRDGNIIMVDDDRMRLLPGEKPQMKK
EECCCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCEEEECCCCEEECCCCCCHHHH
VRFRTLGCYPLTGGVESDAETLDEIIEETLSAVESERTSRVIDHEEAGSMERRKKEGYF
HHHHEECCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA