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The map label for this gene is cysNC [H]

Identifier: 238917812

GI number: 238917812

Start: 1957186

End: 1958826

Strand: Reverse

Name: cysNC [H]

Synonym: EUBELI_01893

Alternate gene names: 238917812

Gene position: 1958826-1957186 (Counterclockwise)

Preceding gene: 238917813

Following gene: 238917811

Centisome position: 91.36

GC content: 42.54

Gene sequence:

>1641_bases
ATGAGGAAGCAGGAAGCATGGAAAGAAGAAAGAAAGAGGGGTACTTCTAGAATGAAAGGTTTACTTAAATTTATTACATG
CGGTAGTGTTGATGATGGAAAATCTACACTTATCGGACACATTTTATATGATTCAAAGTTATTGTATGCTGACCAGGAGA
AGGCGTTGGAGCTTGATTCCAAGGTTGGAAGCCGCGAAGGCAAGATTGATTATTCACTTCTTCTTGATGGTCTTATGGCT
GAGAGAGAGCAGGGAATCACTATTGATGTTGCTTACAGATATTTTACAACTGACAACAGAAGCTTTATCGTTGCTGATAC
TCCGGGACATGAGGAGTACACAAGAAACATGGCGGTTGGTGCTTCATTTGCAGACCTTGCAGTAATCCTTGTTGATGCAA
AGCAGGGCGTGCTTATCCAGACTAAGAGACACGCAAGAATCTGCGCACTCATGGGAATTAAGCATTTTGTATTTGCAGTT
AATAAGATGGACCTTGTTGGTTACAGTGAGGAGCGTTTCAACGAGATTAATGCACAGATAGCAGAGCTTACTAAGGAACT
TTCACTTGCAGATGTAGTTGTTATCCCTGTTTCGGCAACAGAGGGCGACAATGTTACAACTAAGTCTGCTAATATCCCAT
GGTATAAGGGCGAGGCACTTCTTCCATACCTTGAGCAGATTGATGTTGATGATACTGAGGAAGAAAAAGGCTTCTACATG
CCTGTTCAGAGAGTATGCCGTCCAAATCATGAATTCCGTGGTTTCCAGGGACAGATTGAGGCAGGAAGCGTATCTGTTGG
TGACGAGATTATCACTCTTCCAACTAAGGAGCATGTTCATGTCAAGAGCATCCATGTTGGTGATAAGGAAGCCCAGAGCG
CATCTATTGGACAGCCTGTTACTATCCAGTTAGACCGCGAGGTTGATGTCTCAAGAGGTTCAGTTCTTGCAGCAGGAGCA
GACCTTAAGCTTGCAACAGAGATAACAGCTACAATTCTCTGGATGGACGATGATGTTCTTACTAATAACAAGAACTTTTT
TGTAAAGCTTGGAACAAGACTTATTCCGGGCGTTGTTACTGAGATTGTCAATACTATTGATGTCAATACAGGCGAGGAGA
AGCCAGCAGCTCTTCTTAATAAAAATGAAATCGCTGTATGTAAGATTTCTCTTGCTGATGTAATTGTTGTTGATGAATTC
AACCTTCACAAGACTATGGGGGAGCTTATCCTCATAGACCGTGTTACTAATATGACTAGTGCCTGCGGCGTTGTCCGTGA
AGTTAATGAGGCAGCAAGCGATGTTAAGGAAGTTGACGCAGCTTTCAGAGCGGAGCTTAACAACCAGAAGCCTGTTGTTG
TTGAGGCTGTTCTTTCAGACAAGATTAATGTTGATTTCTTAAAGAAGGTTGAGAAGGAGCTTCTTCTTGACAGCAAGCAT
GTATATCTGTATGCTCCGGCAGAAGGCGAGGATTATACAAATGTTGTGACTCACCTTGTAAATGCAGGTATTGTTGTTAT
TCTTGCACTTAAAGAGAAAGCAGATATTAAGATTGATGGTGCAGAAGTCCTTGCAGGCTGGGAGTCTGAGGTTGACGCAA
CTACTGCGGATGTGGCTGAGTTTATTAAGAAGAATGCGTAA

Upstream 100 bases:

>100_bases
CTTACAGGCGGTGTTGAGTCAGACGCAGAAACTCTTGACGAGATTATCGAAGAGACTTTAAGTGCGGTTGAATCAGAGAG
AACAAGCCGTGTTATAGACC

Downstream 100 bases:

>100_bases
GTATTGTACAGAGGTCAGGTTGAGATGAAAAAATATGGATTTTTTAAAAAGTTAATATGTTTAGGTATGATTTTCAGCCT
GACTTTTACCGGACTTTCCG

Product: sulfate adenylyltransferase

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MRKQEAWKEERKRGTSRMKGLLKFITCGSVDDGKSTLIGHILYDSKLLYADQEKALELDSKVGSREGKIDYSLLLDGLMA
EREQGITIDVAYRYFTTDNRSFIVADTPGHEEYTRNMAVGASFADLAVILVDAKQGVLIQTKRHARICALMGIKHFVFAV
NKMDLVGYSEERFNEINAQIAELTKELSLADVVVIPVSATEGDNVTTKSANIPWYKGEALLPYLEQIDVDDTEEEKGFYM
PVQRVCRPNHEFRGFQGQIEAGSVSVGDEIITLPTKEHVHVKSIHVGDKEAQSASIGQPVTIQLDREVDVSRGSVLAAGA
DLKLATEITATILWMDDDVLTNNKNFFVKLGTRLIPGVVTEIVNTIDVNTGEEKPAALLNKNEIAVCKISLADVIVVDEF
NLHKTMGELILIDRVTNMTSACGVVREVNEAASDVKEVDAAFRAELNNQKPVVVEAVLSDKINVDFLKKVEKELLLDSKH
VYLYAPAEGEDYTNVVTHLVNAGIVVILALKEKADIKIDGAEVLAGWESEVDATTADVAEFIKKNA

Sequences:

>Translated_546_residues
MRKQEAWKEERKRGTSRMKGLLKFITCGSVDDGKSTLIGHILYDSKLLYADQEKALELDSKVGSREGKIDYSLLLDGLMA
EREQGITIDVAYRYFTTDNRSFIVADTPGHEEYTRNMAVGASFADLAVILVDAKQGVLIQTKRHARICALMGIKHFVFAV
NKMDLVGYSEERFNEINAQIAELTKELSLADVVVIPVSATEGDNVTTKSANIPWYKGEALLPYLEQIDVDDTEEEKGFYM
PVQRVCRPNHEFRGFQGQIEAGSVSVGDEIITLPTKEHVHVKSIHVGDKEAQSASIGQPVTIQLDREVDVSRGSVLAAGA
DLKLATEITATILWMDDDVLTNNKNFFVKLGTRLIPGVVTEIVNTIDVNTGEEKPAALLNKNEIAVCKISLADVIVVDEF
NLHKTMGELILIDRVTNMTSACGVVREVNEAASDVKEVDAAFRAELNNQKPVVVEAVLSDKINVDFLKKVEKELLLDSKH
VYLYAPAEGEDYTNVVTHLVNAGIVVILALKEKADIKIDGAEVLAGWESEVDATTADVAEFIKKNA
>Mature_546_residues
MRKQEAWKEERKRGTSRMKGLLKFITCGSVDDGKSTLIGHILYDSKLLYADQEKALELDSKVGSREGKIDYSLLLDGLMA
EREQGITIDVAYRYFTTDNRSFIVADTPGHEEYTRNMAVGASFADLAVILVDAKQGVLIQTKRHARICALMGIKHFVFAV
NKMDLVGYSEERFNEINAQIAELTKELSLADVVVIPVSATEGDNVTTKSANIPWYKGEALLPYLEQIDVDDTEEEKGFYM
PVQRVCRPNHEFRGFQGQIEAGSVSVGDEIITLPTKEHVHVKSIHVGDKEAQSASIGQPVTIQLDREVDVSRGSVLAAGA
DLKLATEITATILWMDDDVLTNNKNFFVKLGTRLIPGVVTEIVNTIDVNTGEEKPAALLNKNEIAVCKISLADVIVVDEF
NLHKTMGELILIDRVTNMTSACGVVREVNEAASDVKEVDAAFRAELNNQKPVVVEAVLSDKINVDFLKKVEKELLLDSKH
VYLYAPAEGEDYTNVVTHLVNAGIVVILALKEKADIKIDGAEVLAGWESEVDATTADVAEFIKKNA

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=30.2483069977427, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=30.2483069977427, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI194018522, Length=404, Percent_Identity=26.7326732673267, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI194018520, Length=404, Percent_Identity=26.7326732673267, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI194097354, Length=404, Percent_Identity=26.7326732673267, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI46094014, Length=404, Percent_Identity=26.2376237623762, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI4503475, Length=453, Percent_Identity=26.4900662251656, Blast_Score=146, Evalue=6e-35,
Organism=Homo sapiens, GI4503471, Length=452, Percent_Identity=26.9911504424779, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI34147630, Length=348, Percent_Identity=26.7241379310345, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1789108, Length=446, Percent_Identity=42.152466367713, Blast_Score=356, Evalue=2e-99,
Organism=Escherichia coli, GI2367247, Length=247, Percent_Identity=26.7206477732794, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1790412, Length=337, Percent_Identity=24.0356083086053, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789737, Length=337, Percent_Identity=24.0356083086053, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI32566629, Length=431, Percent_Identity=28.0742459396752, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17552884, Length=445, Percent_Identity=26.9662921348315, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17569207, Length=445, Percent_Identity=26.9662921348315, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI115532067, Length=428, Percent_Identity=26.8691588785047, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=438, Percent_Identity=26.9406392694064, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=25.7990867579909, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=148, Percent_Identity=34.4594594594595, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25141371, Length=254, Percent_Identity=28.3464566929134, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6325337, Length=448, Percent_Identity=26.1160714285714, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=448, Percent_Identity=26.1160714285714, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6322937, Length=448, Percent_Identity=25.6696428571429, Blast_Score=144, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6320377, Length=430, Percent_Identity=26.2790697674419, Blast_Score=123, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6324761, Length=343, Percent_Identity=27.1137026239067, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137380, Length=424, Percent_Identity=27.8301886792453, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45550900, Length=430, Percent_Identity=27.6744186046512, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24652838, Length=461, Percent_Identity=28.4164859002169, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI17137572, Length=461, Percent_Identity=28.4164859002169, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI45553807, Length=445, Percent_Identity=27.6404494382022, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI45553816, Length=445, Percent_Identity=27.6404494382022, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24651721, Length=445, Percent_Identity=27.6404494382022, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI17864154, Length=445, Percent_Identity=27.6404494382022, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI281363316, Length=339, Percent_Identity=27.7286135693215, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI17864358, Length=339, Percent_Identity=27.7286135693215, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19921738, Length=174, Percent_Identity=30.4597701149425, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 60168; Mature: 60168

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKQEAWKEERKRGTSRMKGLLKFITCGSVDDGKSTLIGHILYDSKLLYADQEKALELDS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEEEEECCEEEECCCHHHHHHHH
KVGSREGKIDYSLLLDGLMAEREQGITIDVAYRYFTTDNRSFIVADTPGHEEYTRNMAVG
HCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEECCCCHHHHHHHCCC
ASFADLAVILVDAKQGVLIQTKRHARICALMGIKHFVFAVNKMDLVGYSEERFNEINAQI
CCHHCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHEECCEEEECCCHHHHHHHHHHH
AELTKELSLADVVVIPVSATEGDNVTTKSANIPWYKGEALLPYLEQIDVDDTEEEKGFYM
HHHHHHCCEEEEEEEEEECCCCCCEEEECCCCCEECCCCHHHHHHHCCCCCCCCCCCEEC
PVQRVCRPNHEFRGFQGQIEAGSVSVGDEIITLPTKEHVHVKSIHVGDKEAQSASIGQPV
CHHHHCCCCCCCCCCCCEEECCCEECCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCE
TIQLDREVDVSRGSVLAAGADLKLATEITATILWMDDDVLTNNKNFFVKLGTRLIPGVVT
EEEECCCCCCCCCCEEEECCCEEEEEEEEEEEEEECCCEEECCCEEEEEECCHHHHHHHH
EIVNTIDVNTGEEKPAALLNKNEIAVCKISLADVIVVDEFNLHKTMGELILIDRVTNMTS
HHHHHCCCCCCCCCCHHHCCCCCEEEEEEEEEEEEEEECCCHHHHHCCEEEEEHHHHHHH
ACGVVREVNEAASDVKEVDAAFRAELNNQKPVVVEAVLSDKINVDFLKKVEKELLLDSKH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCE
VYLYAPAEGEDYTNVVTHLVNAGIVVILALKEKADIKIDGAEVLAGWESEVDATTADVAE
EEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCEEECCHHHEECCCCCCCCHHHHHHH
FIKKNA
HHHCCC
>Mature Secondary Structure
MRKQEAWKEERKRGTSRMKGLLKFITCGSVDDGKSTLIGHILYDSKLLYADQEKALELDS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEEEEECCEEEECCCHHHHHHHH
KVGSREGKIDYSLLLDGLMAEREQGITIDVAYRYFTTDNRSFIVADTPGHEEYTRNMAVG
HCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEECCCCHHHHHHHCCC
ASFADLAVILVDAKQGVLIQTKRHARICALMGIKHFVFAVNKMDLVGYSEERFNEINAQI
CCHHCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHEECCEEEECCCHHHHHHHHHHH
AELTKELSLADVVVIPVSATEGDNVTTKSANIPWYKGEALLPYLEQIDVDDTEEEKGFYM
HHHHHHCCEEEEEEEEEECCCCCCEEEECCCCCEECCCCHHHHHHHCCCCCCCCCCCEEC
PVQRVCRPNHEFRGFQGQIEAGSVSVGDEIITLPTKEHVHVKSIHVGDKEAQSASIGQPV
CHHHHCCCCCCCCCCCCEEECCCEECCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCE
TIQLDREVDVSRGSVLAAGADLKLATEITATILWMDDDVLTNNKNFFVKLGTRLIPGVVT
EEEECCCCCCCCCCEEEECCCEEEEEEEEEEEEEECCCEEECCCEEEEEECCHHHHHHHH
EIVNTIDVNTGEEKPAALLNKNEIAVCKISLADVIVVDEFNLHKTMGELILIDRVTNMTS
HHHHHCCCCCCCCCCHHHCCCCCEEEEEEEEEEEEEEECCCHHHHHCCEEEEEHHHHHHH
ACGVVREVNEAASDVKEVDAAFRAELNNQKPVVVEAVLSDKINVDFLKKVEKELLLDSKH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCE
VYLYAPAEGEDYTNVVTHLVNAGIVVILALKEKADIKIDGAEVLAGWESEVDATTADVAE
EEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCEEECCHHHEECCCCCCCCHHHHHHH
FIKKNA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]