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The map label for this gene is sbp [H]

Identifier: 238917811

GI number: 238917811

Start: 1956106

End: 1957161

Strand: Reverse

Name: sbp [H]

Synonym: EUBELI_01892

Alternate gene names: 238917811

Gene position: 1957161-1956106 (Counterclockwise)

Preceding gene: 238917812

Following gene: 238917809

Centisome position: 91.28

GC content: 42.52

Gene sequence:

>1056_bases
ATGAAAAAATATGGATTTTTTAAAAAGTTAATATGTTTAGGTATGATTTTCAGCCTGACTTTTACCGGACTTTCCGGGTG
TGGGCAGAAGAAGACGGAACTTGTTAATGTTTCTTATGATGCTACCAGAGAGTTTTATAAGGAGTATAACAGGCTTTTTG
AAGATTACTGGTATGATAAGACTGGTGAGAAGGTTGAGGTTATCCAGTCTCATGGCGGTTCTGGAAAGCAGGCGCTAGAG
GTTGCTAACGGTCTTGGAGCTGATGTTGTCACGCTTGCTCTTGAGGGTGATGTTAATGTTATAAGGAACGAAGGGCTGAT
TGATGACGGATATATTGATGATTATTCTGATGACAGCTCGCCTTACACTTCTACGATTGTCTTTCTTGTAAGAAAGGGTA
ATCCTAAGAATATTAAGGACTGGGATGACCTTCTTAATGACGGGGTTAAGGTTATTACCCCTAATCCTAAGACTTCTGGA
GGAGCCAGATGGAATTTCCTTGCGGCATGGTATTATTTTAAGAAGCAGGGACAGACTGATGAACAGGTTCAGGCAAGTGT
TACCAAGCTTTACAAGAATGTGGCAGTGCTTGATTCGGGTGCAAGAGGTTCTTCTACAACATTTGCAGAGAACGGACAGG
GGGATGTTCTGATTGCATGGGAAAATGAAGCATTTTTCGCATTACAGGAATATCCGGGCGAGTATGAGATTGTTGTTCCG
TCCGCATCTGTATTGTGCCAGCCAACGGTTGCGAAGGTTGACGAGGTCACACAGATGAATGGAACTGCGGAACTGGCAGA
CGCCTATCTTTCTTTCCTGTACACTGATGATGCGCAGAGACTTGAAGCACAGAACTTCTACAGACCTGTAAACAAGGACA
TCCAGAAGGAATACGAATCTTCAAGTGACGCAAGAAATATTAAAGAGATTCTATCTGATGGCAAATGGATTGTCAGCGAC
ATTGACATGGCAGATATCGGGTATTTTGGCGGTTGGGAAGCGGCTACACAGAAGTTTTTCTCTGATGGCGGAATATTTGA
TAAGATATATGATTAA

Upstream 100 bases:

>100_bases
AGTCCTTGCAGGCTGGGAGTCTGAGGTTGACGCAACTACTGCGGATGTGGCTGAGTTTATTAAGAAGAATGCGTAAGTAT
TGTACAGAGGTCAGGTTGAG

Downstream 100 bases:

>100_bases
TTAAAATGTAAGGTCACACTTAACCGGCTTTGAGCTGGCTGAGTGTGGTCTTTTTTGTGTGAATCTGACTATGCGCAGAC
GCATGAAGCCTACTAACATA

Product: sulfate transport system substrate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKKYGFFKKLICLGMIFSLTFTGLSGCGQKKTELVNVSYDATREFYKEYNRLFEDYWYDKTGEKVEVIQSHGGSGKQALE
VANGLGADVVTLALEGDVNVIRNEGLIDDGYIDDYSDDSSPYTSTIVFLVRKGNPKNIKDWDDLLNDGVKVITPNPKTSG
GARWNFLAAWYYFKKQGQTDEQVQASVTKLYKNVAVLDSGARGSSTTFAENGQGDVLIAWENEAFFALQEYPGEYEIVVP
SASVLCQPTVAKVDEVTQMNGTAELADAYLSFLYTDDAQRLEAQNFYRPVNKDIQKEYESSSDARNIKEILSDGKWIVSD
IDMADIGYFGGWEAATQKFFSDGGIFDKIYD

Sequences:

>Translated_351_residues
MKKYGFFKKLICLGMIFSLTFTGLSGCGQKKTELVNVSYDATREFYKEYNRLFEDYWYDKTGEKVEVIQSHGGSGKQALE
VANGLGADVVTLALEGDVNVIRNEGLIDDGYIDDYSDDSSPYTSTIVFLVRKGNPKNIKDWDDLLNDGVKVITPNPKTSG
GARWNFLAAWYYFKKQGQTDEQVQASVTKLYKNVAVLDSGARGSSTTFAENGQGDVLIAWENEAFFALQEYPGEYEIVVP
SASVLCQPTVAKVDEVTQMNGTAELADAYLSFLYTDDAQRLEAQNFYRPVNKDIQKEYESSSDARNIKEILSDGKWIVSD
IDMADIGYFGGWEAATQKFFSDGGIFDKIYD
>Mature_351_residues
MKKYGFFKKLICLGMIFSLTFTGLSGCGQKKTELVNVSYDATREFYKEYNRLFEDYWYDKTGEKVEVIQSHGGSGKQALE
VANGLGADVVTLALEGDVNVIRNEGLIDDGYIDDYSDDSSPYTSTIVFLVRKGNPKNIKDWDDLLNDGVKVITPNPKTSG
GARWNFLAAWYYFKKQGQTDEQVQASVTKLYKNVAVLDSGARGSSTTFAENGQGDVLIAWENEAFFALQEYPGEYEIVVP
SASVLCQPTVAKVDEVTQMNGTAELADAYLSFLYTDDAQRLEAQNFYRPVNKDIQKEYESSSDARNIKEILSDGKWIVSD
IDMADIGYFGGWEAATQKFFSDGGIFDKIYD

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=351, Percent_Identity=49.5726495726496, Blast_Score=330, Evalue=9e-92,
Organism=Escherichia coli, GI1788765, Length=322, Percent_Identity=40.0621118012422, Blast_Score=223, Evalue=1e-59,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39217; Mature: 39217

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYGFFKKLICLGMIFSLTFTGLSGCGQKKTELVNVSYDATREFYKEYNRLFEDYWYDK
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCC
TGEKVEVIQSHGGSGKQALEVANGLGADVVTLALEGDVNVIRNEGLIDDGYIDDYSDDSS
CCCEEHHHHCCCCCCHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCC
PYTSTIVFLVRKGNPKNIKDWDDLLNDGVKVITPNPKTSGGARWNFLAAWYYFKKQGQTD
CCEEEEEEEEECCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCHHHHHHEEHHHHCCCCH
EQVQASVTKLYKNVAVLDSGARGSSTTFAENGQGDVLIAWENEAFFALQEYPGEYEIVVP
HHHHHHHHHHHHHHHEEECCCCCCCCEECCCCCCCEEEEECCCEEEEEECCCCCEEEEEC
SASVLCQPTVAKVDEVTQMNGTAELADAYLSFLYTDDAQRLEAQNFYRPVNKDIQKEYES
CCHHEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHC
SSDARNIKEILSDGKWIVSDIDMADIGYFGGWEAATQKFFSDGGIFDKIYD
CCHHHHHHHHHCCCCEEEEECCHHHCCCCCCHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MKKYGFFKKLICLGMIFSLTFTGLSGCGQKKTELVNVSYDATREFYKEYNRLFEDYWYDK
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCC
TGEKVEVIQSHGGSGKQALEVANGLGADVVTLALEGDVNVIRNEGLIDDGYIDDYSDDSS
CCCEEHHHHCCCCCCHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCC
PYTSTIVFLVRKGNPKNIKDWDDLLNDGVKVITPNPKTSGGARWNFLAAWYYFKKQGQTD
CCEEEEEEEEECCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCHHHHHHEEHHHHCCCCH
EQVQASVTKLYKNVAVLDSGARGSSTTFAENGQGDVLIAWENEAFFALQEYPGEYEIVVP
HHHHHHHHHHHHHHHEEECCCCCCCCEECCCCCCCEEEEECCCEEEEEECCCCCEEEEEC
SASVLCQPTVAKVDEVTQMNGTAELADAYLSFLYTDDAQRLEAQNFYRPVNKDIQKEYES
CCHHEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHC
SSDARNIKEILSDGKWIVSDIDMADIGYFGGWEAATQKFFSDGGIFDKIYD
CCHHHHHHHHHCCCCEEEEECCHHHCCCCCCHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]