BacMap

Definition	Eubacterium eligens ATCC 27750 chromosome, complete genome.
Accession	NC_012778
Length	2,144,190

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The map label for this gene is ppiA [C]

Identifier: 238917748

GI number: 238917748

Start: 1888609

End: 1889148

Strand: Direct

Name: ppiA [C]

Synonym: EUBELI_01829

Alternate gene names: 238917748

Gene position: 1888609-1889148 (Clockwise)

Preceding gene: 238917746

Following gene: 238917752

Centisome position: 88.08

GC content: 41.85

Gene sequence:

>540_bases
ATGAATTTCAGGAGGAATAATATGGCTAATCCAATAGTTACATTTACAATGGAAAACGGCGATGTGATGAAAGCTGAGCT
TTATCCGGAAATCGCACCTACATCAGTTAATAATTTCGTTAATCTTGTTCAGAAGGGATTCTATGACGGACTTATATTTC
ACAGAGTAATCGCAGGTTTTATGATTCAGGGTGGTGACCCTGAAGGAACAGGTATGGGTGGTCCTGGCTACTCTATCAAG
GGCGAATTCGCATCTAACGGATTTAAGAACGACTTAAAGCATACAAGAGGTGTTCTTTCAATGGCTCGTTCTATGAGACC
TGATTCTGCAGGTTCACAGTTCTTTATCATGCACCAGAACGCTCCACATCTTGACGGAGATTACGCTGCTTTTGGTAAGC
TTATCGAGGGTGAAGATGTTCTTGACAAGATTGCAGGCGTAGACACAGATTACAGCGACAGACCTAGAAAACCACAGGTT
ATGAAGACTGTTACTGTTGAGACATTTGGCGAGGAATATCCAGAACCAGAGAAGTTATAA

Upstream 100 bases:

>100_bases
TCTTCATAATTGTTTCTAAAGTTTTAATTTGACTTTTTATACTGTAGTATTTAGAATTAATTACATCACATTTTGCTCAT
GCAGACAAGCGGGCAATTTA

Downstream 100 bases:

>100_bases
TTAAATTATATTAAACAAAATCGGCGGGTACATATCACAATTCTGTACCCGCCTTTATTATTATATGTTTATCATCTAGA
AATGTCCGCCGTAGCCACTG

Product: peptidylprolyl isomerase

Products: NA

Alternate protein names: Phosphatase; Peptidyl-prolyl cis-trans isomerase; PPIase; Rotamase [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MNFRRNNMANPIVTFTMENGDVMKAELYPEIAPTSVNNFVNLVQKGFYDGLIFHRVIAGFMIQGGDPEGTGMGGPGYSIK
GEFASNGFKNDLKHTRGVLSMARSMRPDSAGSQFFIMHQNAPHLDGDYAAFGKLIEGEDVLDKIAGVDTDYSDRPRKPQV
MKTVTVETFGEEYPEPEKL

Sequences:

>Translated_179_residues
MNFRRNNMANPIVTFTMENGDVMKAELYPEIAPTSVNNFVNLVQKGFYDGLIFHRVIAGFMIQGGDPEGTGMGGPGYSIK
GEFASNGFKNDLKHTRGVLSMARSMRPDSAGSQFFIMHQNAPHLDGDYAAFGKLIEGEDVLDKIAGVDTDYSDRPRKPQV
MKTVTVETFGEEYPEPEKL
>Mature_179_residues
MNFRRNNMANPIVTFTMENGDVMKAELYPEIAPTSVNNFVNLVQKGFYDGLIFHRVIAGFMIQGGDPEGTGMGGPGYSIK
GEFASNGFKNDLKHTRGVLSMARSMRPDSAGSQFFIMHQNAPHLDGDYAAFGKLIEGEDVLDKIAGVDTDYSDRPRKPQV
MKTVTVETFGEEYPEPEKL

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI19557636, Length=157, Percent_Identity=41.4012738853503, Blast_Score=111, Evalue=3e-25,
Organism=Homo sapiens, GI7706339, Length=135, Percent_Identity=42.2222222222222, Blast_Score=105, Evalue=1e-23,
Organism=Homo sapiens, GI24308049, Length=150, Percent_Identity=39.3333333333333, Blast_Score=98, Evalue=5e-21,
Organism=Homo sapiens, GI64276486, Length=161, Percent_Identity=37.2670807453416, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI22547212, Length=156, Percent_Identity=41.6666666666667, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI7657473, Length=156, Percent_Identity=41.6666666666667, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI5454154, Length=169, Percent_Identity=38.4615384615385, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI22547215, Length=165, Percent_Identity=41.2121212121212, Blast_Score=91, Evalue=4e-19,
Organism=Homo sapiens, GI4758950, Length=141, Percent_Identity=44.6808510638298, Blast_Score=88, Evalue=3e-18,
Organism=Homo sapiens, GI6631100, Length=146, Percent_Identity=41.7808219178082, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI4505991, Length=134, Percent_Identity=47.7611940298507, Blast_Score=86, Evalue=1e-17,
Organism=Homo sapiens, GI42560244, Length=146, Percent_Identity=38.3561643835616, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI10863927, Length=176, Percent_Identity=37.5, Blast_Score=78, Evalue=4e-15,
Organism=Homo sapiens, GI4826932, Length=176, Percent_Identity=37.5, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI27735045, Length=142, Percent_Identity=40.8450704225352, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI20911035, Length=141, Percent_Identity=36.1702127659575, Blast_Score=73, Evalue=1e-13,
Organism=Homo sapiens, GI5031987, Length=127, Percent_Identity=44.8818897637795, Blast_Score=73, Evalue=1e-13,
Organism=Homo sapiens, GI310127646, Length=145, Percent_Identity=36.551724137931, Blast_Score=73, Evalue=1e-13,
Organism=Homo sapiens, GI310114371, Length=145, Percent_Identity=36.551724137931, Blast_Score=73, Evalue=1e-13,
Organism=Homo sapiens, GI310119221, Length=145, Percent_Identity=36.551724137931, Blast_Score=73, Evalue=1e-13,
Organism=Homo sapiens, GI303305009, Length=126, Percent_Identity=43.6507936507937, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI45439316, Length=126, Percent_Identity=43.6507936507937, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI5174637, Length=126, Percent_Identity=43.6507936507937, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI289803023, Length=165, Percent_Identity=34.5454545454545, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI256017253, Length=149, Percent_Identity=38.255033557047, Blast_Score=71, Evalue=5e-13,
Organism=Homo sapiens, GI221139811, Length=149, Percent_Identity=38.255033557047, Blast_Score=71, Evalue=5e-13,
Organism=Homo sapiens, GI256017251, Length=149, Percent_Identity=38.255033557047, Blast_Score=71, Evalue=5e-13,
Organism=Homo sapiens, GI310110104, Length=124, Percent_Identity=42.741935483871, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI150418007, Length=147, Percent_Identity=39.4557823129252, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI310129267, Length=124, Percent_Identity=41.9354838709677, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI310120620, Length=124, Percent_Identity=41.9354838709677, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI178057341, Length=125, Percent_Identity=41.6, Blast_Score=68, Evalue=4e-12,
Organism=Escherichia coli, GI1789763, Length=167, Percent_Identity=43.7125748502994, Blast_Score=105, Evalue=1e-24,
Organism=Escherichia coli, GI1786736, Length=169, Percent_Identity=40.2366863905325, Blast_Score=104, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17539496, Length=132, Percent_Identity=48.4848484848485, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71980594, Length=156, Percent_Identity=42.9487179487179, Blast_Score=113, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71980590, Length=148, Percent_Identity=43.9189189189189, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17532641, Length=160, Percent_Identity=38.125, Blast_Score=95, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17506311, Length=145, Percent_Identity=41.3793103448276, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17551284, Length=141, Percent_Identity=41.1347517730496, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17551282, Length=141, Percent_Identity=41.1347517730496, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17506307, Length=141, Percent_Identity=45.3900709219858, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71997782, Length=148, Percent_Identity=39.1891891891892, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17552780, Length=141, Percent_Identity=43.9716312056738, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17552784, Length=163, Percent_Identity=36.1963190184049, Blast_Score=81, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17566266, Length=134, Percent_Identity=43.2835820895522, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17565860, Length=167, Percent_Identity=38.9221556886228, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17552782, Length=162, Percent_Identity=38.8888888888889, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17559074, Length=132, Percent_Identity=41.6666666666667, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539498, Length=156, Percent_Identity=37.8205128205128, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17532643, Length=169, Percent_Identity=33.7278106508876, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32563659, Length=145, Percent_Identity=31.7241379310345, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321848, Length=158, Percent_Identity=41.7721518987342, Blast_Score=89, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323562, Length=143, Percent_Identity=41.958041958042, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320359, Length=124, Percent_Identity=43.5483870967742, Blast_Score=79, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320510, Length=144, Percent_Identity=41.6666666666667, Blast_Score=78, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323246, Length=171, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19922376, Length=145, Percent_Identity=42.0689655172414, Blast_Score=119, Evalue=9e-28,
Organism=Drosophila melanogaster, GI17986117, Length=158, Percent_Identity=43.0379746835443, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24652460, Length=157, Percent_Identity=37.5796178343949, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24650807, Length=143, Percent_Identity=39.1608391608392, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI20130249, Length=141, Percent_Identity=43.9716312056738, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21357249, Length=133, Percent_Identity=38.3458646616541, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45549139, Length=127, Percent_Identity=44.8818897637795, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17136360, Length=159, Percent_Identity=37.1069182389937, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24586038, Length=179, Percent_Identity=34.6368715083799, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24664125, Length=127, Percent_Identity=43.3070866141732, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357783, Length=127, Percent_Identity=43.3070866141732, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922912, Length=145, Percent_Identity=39.3103448275862, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21355677, Length=167, Percent_Identity=31.7365269461078, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150
- InterPro:   IPR002130 [H]

Pfam domain/function: PF08282 Hydrolase_3; PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 19806; Mature: 19806

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRRNNMANPIVTFTMENGDVMKAELYPEIAPTSVNNFVNLVQKGFYDGLIFHRVIAGF
CCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MIQGGDPEGTGMGGPGYSIKGEFASNGFKNDLKHTRGVLSMARSMRPDSAGSQFFIMHQN
HCCCCCCCCCCCCCCCCEEECHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
APHLDGDYAAFGKLIEGEDVLDKIAGVDTDYSDRPRKPQVMKTVTVETFGEEYPEPEKL
CCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHEEHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNFRRNNMANPIVTFTMENGDVMKAELYPEIAPTSVNNFVNLVQKGFYDGLIFHRVIAGF
CCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MIQGGDPEGTGMGGPGYSIKGEFASNGFKNDLKHTRGVLSMARSMRPDSAGSQFFIMHQN
HCCCCCCCCCCCCCCCCEEECHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
APHLDGDYAAFGKLIEGEDVLDKIAGVDTDYSDRPRKPQVMKTVTVETFGEEYPEPEKL
CCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHEEHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA