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The map label for this gene is hphIAM [H]

Identifier: 238917713

GI number: 238917713

Start: 1853605

End: 1855038

Strand: Reverse

Name: hphIAM [H]

Synonym: EUBELI_01793

Alternate gene names: 238917713

Gene position: 1855038-1853605 (Counterclockwise)

Preceding gene: 238917714

Following gene: 238917712

Centisome position: 86.51

GC content: 35.56

Gene sequence:

>1434_bases
ATGGAATACAAAAATGATAAAGGTGAATTTATATCTTTTATAAAGGCTGGATTGGGCGATAAGGAGAATAATCATGTAGA
TGTCTGCCTAAAAAGAGTTACAAAGCCTGTAACAGAGAAATTCCTTAATGACAGGGCAGATCTATATAAGACTGATGTGG
ATAATGATTCGGAACTTATGGAAGCATTTAGAAGACTGGCTTTTATATGCAATGAAAAAGAACAGGAATGCTATGAGTGT
CCTGTAAGTAAATATTGTAATACATATATTGAAAATGCCAGAAAAAATGTAAGTGATGATTCGTTAAAGATGTTAGATCT
GTTTTGCGGGGCTGGTGGCTTGTCATTAGGTTTTACACAGGAAGGATTTGTGACAAGTCTGGCAAATGATATACAGGACT
GTTGTGTGGACACATATGCTCACAATCATCCGGAGACACCAAGAGATCACATAGTGCTAGGTGATATAAAAGATGTGGTA
AAAAATCTTGATGAGCTACTGGCAGGCAGGAACGTTGATATTGTTGTTGGTGGACCACCATGTCAGGGATTTAGTATGGC
TAACAGACAGAGATTGATAGATGATCCTAGAAATCATCTGTATAAGAGCTATGTTGAAGTTGTAAAAAAAGTACACCCTA
AATTTTTTGTTATGGAAAATGTTAAAGGAATGTTATCTGTAGCTGAACAGGTAAAAGAAGATTTTCGTGCCATCGGATAT
TCGGTAGAATGCCATATATTAAATGCCAAGAATTTTGGGGTTCCACAGAACAGGGAAAGACTTATATATATAGGAAATTG
CATGGGAGTAGATAACGAACAAATTTTTAATGAAATATTTGCACTAAGTGAAAATATACCAGAACATAATCTTGGAGACG
CGCTATTTGCACTTAGGGAACTGGAGGCTTCAAGAGTAAAAAATTCTACAGAAAGTGGTTCAATAGAGAGCGGATACAAA
ATAGAAAAAAATAATATTACAGAAACCAATGATTATATTTCATATATTAATCAGGATAAGGTGGTTAATGTAGTATCAAA
TCATAAAGCGAGATATAATAATGATCGTGATATAGAAATTTATGGCCGCATGGAGCCGGGAGACCGCTCAGATGATCCAA
AAATTGCAGATATTATGCCATATGCCAGAAGAAATGGTATTTTTAAAGATAAATATTTTAAACTTGAAAATGACAAGGTT
TGCAAAACAATAACCGCACATATGAAGTTCGATTGTAATATGTATATTCATCCAACACAGGCAAGAGGACTCACACCAAG
GGAGGCAGCAAGGGTGCAGTCTTATCCTGATGATTATTTTTTCAGAGGTGCATATACGAAGACTTATATGCAGATAGGAA
ATTCTGTTCCTCCACTTCTGGGAAGAGCGATAGCACATGTGATAAAAAGCTATATGAAAGGTGGTAATGACTGA

Upstream 100 bases:

>100_bases
TATAAACAGTTTGGAAATGCAGTTACAGTTAATGTATCAAAAGCTGTTGCCCAATCGGTAAAGGCAACATTAATAAATCT
TGGAGAGTGGAAAGACTGAT

Downstream 100 bases:

>100_bases
TGGAGTTTACAGAGGCAATAGAGATAATAAAGAACATAGTTCCGGAGAGTTCAGCAAATGAATATGATGGAATAATGGTA
AAAAGACTGGAAGCATCTAA

Product: DNA (cytosine-5-)-methyltransferase

Products: NA

Alternate protein names: M.HphIA; Cytosine-specific methyltransferase HphIA; M.Hphi(C) [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MEYKNDKGEFISFIKAGLGDKENNHVDVCLKRVTKPVTEKFLNDRADLYKTDVDNDSELMEAFRRLAFICNEKEQECYEC
PVSKYCNTYIENARKNVSDDSLKMLDLFCGAGGLSLGFTQEGFVTSLANDIQDCCVDTYAHNHPETPRDHIVLGDIKDVV
KNLDELLAGRNVDIVVGGPPCQGFSMANRQRLIDDPRNHLYKSYVEVVKKVHPKFFVMENVKGMLSVAEQVKEDFRAIGY
SVECHILNAKNFGVPQNRERLIYIGNCMGVDNEQIFNEIFALSENIPEHNLGDALFALRELEASRVKNSTESGSIESGYK
IEKNNITETNDYISYINQDKVVNVVSNHKARYNNDRDIEIYGRMEPGDRSDDPKIADIMPYARRNGIFKDKYFKLENDKV
CKTITAHMKFDCNMYIHPTQARGLTPREAARVQSYPDDYFFRGAYTKTYMQIGNSVPPLLGRAIAHVIKSYMKGGND

Sequences:

>Translated_477_residues
MEYKNDKGEFISFIKAGLGDKENNHVDVCLKRVTKPVTEKFLNDRADLYKTDVDNDSELMEAFRRLAFICNEKEQECYEC
PVSKYCNTYIENARKNVSDDSLKMLDLFCGAGGLSLGFTQEGFVTSLANDIQDCCVDTYAHNHPETPRDHIVLGDIKDVV
KNLDELLAGRNVDIVVGGPPCQGFSMANRQRLIDDPRNHLYKSYVEVVKKVHPKFFVMENVKGMLSVAEQVKEDFRAIGY
SVECHILNAKNFGVPQNRERLIYIGNCMGVDNEQIFNEIFALSENIPEHNLGDALFALRELEASRVKNSTESGSIESGYK
IEKNNITETNDYISYINQDKVVNVVSNHKARYNNDRDIEIYGRMEPGDRSDDPKIADIMPYARRNGIFKDKYFKLENDKV
CKTITAHMKFDCNMYIHPTQARGLTPREAARVQSYPDDYFFRGAYTKTYMQIGNSVPPLLGRAIAHVIKSYMKGGND
>Mature_477_residues
MEYKNDKGEFISFIKAGLGDKENNHVDVCLKRVTKPVTEKFLNDRADLYKTDVDNDSELMEAFRRLAFICNEKEQECYEC
PVSKYCNTYIENARKNVSDDSLKMLDLFCGAGGLSLGFTQEGFVTSLANDIQDCCVDTYAHNHPETPRDHIVLGDIKDVV
KNLDELLAGRNVDIVVGGPPCQGFSMANRQRLIDDPRNHLYKSYVEVVKKVHPKFFVMENVKGMLSVAEQVKEDFRAIGY
SVECHILNAKNFGVPQNRERLIYIGNCMGVDNEQIFNEIFALSENIPEHNLGDALFALRELEASRVKNSTESGSIESGYK
IEKNNITETNDYISYINQDKVVNVVSNHKARYNNDRDIEIYGRMEPGDRSDDPKIADIMPYARRNGIFKDKYFKLENDKV
CKTITAHMKFDCNMYIHPTQARGLTPREAARVQSYPDDYFFRGAYTKTYMQIGNSVPPLLGRAIAHVIKSYMKGGND

Specific function: This methylase protects the DNA from cleavage by the HphI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI195927037, Length=189, Percent_Identity=30.6878306878307, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI4503351, Length=189, Percent_Identity=30.6878306878307, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1788271, Length=199, Percent_Identity=26.1306532663317, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 54339; Mature: 54339

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYKNDKGEFISFIKAGLGDKENNHVDVCLKRVTKPVTEKFLNDRADLYKTDVDNDSELM
CCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHH
EAFRRLAFICNEKEQECYECPVSKYCNTYIENARKNVSDDSLKMLDLFCGAGGLSLGFTQ
HHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
EGFVTSLANDIQDCCVDTYAHNHPETPRDHIVLGDIKDVVKNLDELLAGRNVDIVVGGPP
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCEEEECCCC
CQGFSMANRQRLIDDPRNHLYKSYVEVVKKVHPKFFVMENVKGMLSVAEQVKEDFRAIGY
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCC
SVECHILNAKNFGVPQNRERLIYIGNCMGVDNEQIFNEIFALSENIPEHNLGDALFALRE
EEEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LEASRVKNSTESGSIESGYKIEKNNITETNDYISYINQDKVVNVVSNHKARYNNDRDIEI
HHHHHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEE
YGRMEPGDRSDDPKIADIMPYARRNGIFKDKYFKLENDKVCKTITAHMKFDCNMYIHPTQ
EEECCCCCCCCCCCHHHHCCHHHHCCCCCCCCEECCCCHHHHHHHHHEEECCEEEECCHH
ARGLTPREAARVQSYPDDYFFRGAYTKTYMQIGNSVPPLLGRAIAHVIKSYMKGGND
CCCCCHHHHHHHCCCCCCCEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEYKNDKGEFISFIKAGLGDKENNHVDVCLKRVTKPVTEKFLNDRADLYKTDVDNDSELM
CCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHH
EAFRRLAFICNEKEQECYECPVSKYCNTYIENARKNVSDDSLKMLDLFCGAGGLSLGFTQ
HHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
EGFVTSLANDIQDCCVDTYAHNHPETPRDHIVLGDIKDVVKNLDELLAGRNVDIVVGGPP
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCEEEECCCC
CQGFSMANRQRLIDDPRNHLYKSYVEVVKKVHPKFFVMENVKGMLSVAEQVKEDFRAIGY
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCC
SVECHILNAKNFGVPQNRERLIYIGNCMGVDNEQIFNEIFALSENIPEHNLGDALFALRE
EEEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LEASRVKNSTESGSIESGYKIEKNNITETNDYISYINQDKVVNVVSNHKARYNNDRDIEI
HHHHHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEE
YGRMEPGDRSDDPKIADIMPYARRNGIFKDKYFKLENDKVCKTITAHMKFDCNMYIHPTQ
EEECCCCCCCCCCCHHHHCCHHHHCCCCCCCCEECCCCHHHHHHHHHEEECCEEEECCHH
ARGLTPREAARVQSYPDDYFFRGAYTKTYMQIGNSVPPLLGRAIAHVIKSYMKGGND
CCCCCHHHHHHHCCCCCCCEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759008 [H]