Click here to switch to the map view.

The map label for this gene is nlaXM [H]

Identifier: 238917714

GI number: 238917714

Start: 1855040

End: 1855975

Strand: Reverse

Name: nlaXM [H]

Synonym: EUBELI_01794

Alternate gene names: 238917714

Gene position: 1855975-1855040 (Counterclockwise)

Preceding gene: 238917715

Following gene: 238917713

Centisome position: 86.56

GC content: 36.0

Gene sequence:

>936_bases
ATGAAAGTAGTTTCATTCTTCAGTGGGCTTGGTGGACTTGATAAAGGGTTTGTAGATACAGGCTATGATATAATATGGGC
AAATGATTTTGATAAATATGCTGTGCAGACATATAAGGCTAATTTTGGAGAACATATTGTTCTTGGAGATATAAATGAAA
TTCCATTAGAGGAGATACCAGACTGCGATATACTGATAGGTGGTTTTCCGTGCCAGCCGTTTAGTATGATGGGGCAGCAA
AAAGGGTTTGAAGATGCAAGAGGAACATTGTTTTTCAGAATAGCAGAGATTGTGGATGATAAAATAAAAAAAGGAAAGAA
ACCGAAGGCAATTATTCTTGAAAATGTAAGATCCTTGAGAACACATAATAATGGAGAAACATATAAAGAAATATACAGAA
TATTACATGATGTGCTTGGATATAATGTGTTCTGTGACATATTAAATTCAGCTGATTATGGAGTACCTCAGACACGAAAC
AGAACATATATTGTATGTTTTGATAATCAGAATGCAAGATTTGAATTTCCAGAAAAGAAAAAACTTAATAAAACATTGCA
GGATTTACTGGAACCAGAAGTTGATGATAAATATTTCCTGTCAGATAGAATATTGCCTACAATATTATCAGATGGAACTG
GTGGATATAAGGCAAAATCGGAAATAGATCTGAAGATTGCAAGACCTTTGTGTGCAACTATGGCGAAGATGCACAGAGCA
TGCCAGGATAACTATGTAACCCAGAATGGAAGGGTAAGAAGACTTACTCCGCGTGAGTGTGCAAGATTGCAGGGATTTCA
GGATTCATTCGTAATCCCGGTATCTGACAGTCAGGCATATAAACAGTTTGGAAATGCAGTTACAGTTAATGTATCAAAAG
CTGTTGCCCAATCGGTAAAGGCAACATTAATAAATCTTGGAGAGTGGAAAGACTGA

Upstream 100 bases:

>100_bases
TATGACATTATTGCGTAATGAAGGGAAAACTGCCTCTGTGGAAATTGGATTGTGGATCAGAGATTACATAAAAAATAATA
AGCTTGAGGAGGAAAAATAA

Downstream 100 bases:

>100_bases
TATGGAATACAAAAATGATAAAGGTGAATTTATATCTTTTATAAAGGCTGGATTGGGCGATAAGGAGAATAATCATGTAG
ATGTCTGCCTAAAAAGAGTT

Product: DNA (cytosine-5-)-methyltransferase

Products: NA

Alternate protein names: M.NlaX [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKVVSFFSGLGGLDKGFVDTGYDIIWANDFDKYAVQTYKANFGEHIVLGDINEIPLEEIPDCDILIGGFPCQPFSMMGQQ
KGFEDARGTLFFRIAEIVDDKIKKGKKPKAIILENVRSLRTHNNGETYKEIYRILHDVLGYNVFCDILNSADYGVPQTRN
RTYIVCFDNQNARFEFPEKKKLNKTLQDLLEPEVDDKYFLSDRILPTILSDGTGGYKAKSEIDLKIARPLCATMAKMHRA
CQDNYVTQNGRVRRLTPRECARLQGFQDSFVIPVSDSQAYKQFGNAVTVNVSKAVAQSVKATLINLGEWKD

Sequences:

>Translated_311_residues
MKVVSFFSGLGGLDKGFVDTGYDIIWANDFDKYAVQTYKANFGEHIVLGDINEIPLEEIPDCDILIGGFPCQPFSMMGQQ
KGFEDARGTLFFRIAEIVDDKIKKGKKPKAIILENVRSLRTHNNGETYKEIYRILHDVLGYNVFCDILNSADYGVPQTRN
RTYIVCFDNQNARFEFPEKKKLNKTLQDLLEPEVDDKYFLSDRILPTILSDGTGGYKAKSEIDLKIARPLCATMAKMHRA
CQDNYVTQNGRVRRLTPRECARLQGFQDSFVIPVSDSQAYKQFGNAVTVNVSKAVAQSVKATLINLGEWKD
>Mature_311_residues
MKVVSFFSGLGGLDKGFVDTGYDIIWANDFDKYAVQTYKANFGEHIVLGDINEIPLEEIPDCDILIGGFPCQPFSMMGQQ
KGFEDARGTLFFRIAEIVDDKIKKGKKPKAIILENVRSLRTHNNGETYKEIYRILHDVLGYNVFCDILNSADYGVPQTRN
RTYIVCFDNQNARFEFPEKKKLNKTLQDLLEPEVDDKYFLSDRILPTILSDGTGGYKAKSEIDLKIARPLCATMAKMHRA
CQDNYVTQNGRVRRLTPRECARLQGFQDSFVIPVSDSQAYKQFGNAVTVNVSKAVAQSVKATLINLGEWKD

Specific function: This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI195927037, Length=221, Percent_Identity=27.6018099547511, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI4503351, Length=221, Percent_Identity=27.6018099547511, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1788271, Length=360, Percent_Identity=32.5, Blast_Score=154, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 35182; Mature: 35182

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVSFFSGLGGLDKGFVDTGYDIIWANDFDKYAVQTYKANFGEHIVLGDINEIPLEEIP
CCHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHCC
DCDILIGGFPCQPFSMMGQQKGFEDARGTLFFRIAEIVDDKIKKGKKPKAIILENVRSLR
CCCEEECCCCCCHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
THNNGETYKEIYRILHDVLGYNVFCDILNSADYGVPQTRNRTYIVCFDNQNARFEFPEKK
CCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHH
KLNKTLQDLLEPEVDDKYFLSDRILPTILSDGTGGYKAKSEIDLKIARPLCATMAKMHRA
HHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
CQDNYVTQNGRVRRLTPRECARLQGFQDSFVIPVSDSQAYKQFGNAVTVNVSKAVAQSVK
HHHCCCCCCCCEEECCHHHHHHHCCCCCCEEEECCCCHHHHHHCCEEEEEHHHHHHHHHH
ATLINLGEWKD
HHHHHCCCCCC
>Mature Secondary Structure
MKVVSFFSGLGGLDKGFVDTGYDIIWANDFDKYAVQTYKANFGEHIVLGDINEIPLEEIP
CCHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHCC
DCDILIGGFPCQPFSMMGQQKGFEDARGTLFFRIAEIVDDKIKKGKKPKAIILENVRSLR
CCCEEECCCCCCHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
THNNGETYKEIYRILHDVLGYNVFCDILNSADYGVPQTRNRTYIVCFDNQNARFEFPEKK
CCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHH
KLNKTLQDLLEPEVDDKYFLSDRILPTILSDGTGGYKAKSEIDLKIARPLCATMAKMHRA
HHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
CQDNYVTQNGRVRRLTPRECARLQGFQDSFVIPVSDSQAYKQFGNAVTVNVSKAVAQSVK
HHHCCCCCCCCEEECCHHHHHHHCCCCCCEEEECCCCHHHHHHCCEEEEEHHHHHHHHHH
ATLINLGEWKD
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2277628 [H]