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The map label for this gene is pilT [H]

Identifier: 238917624

GI number: 238917624

Start: 1768300

End: 1769349

Strand: Reverse

Name: pilT [H]

Synonym: EUBELI_01703

Alternate gene names: 238917624

Gene position: 1769349-1768300 (Counterclockwise)

Preceding gene: 238917625

Following gene: 238917623

Centisome position: 82.52

GC content: 37.81

Gene sequence:

>1050_bases
ATGGAATTAAAACAGTTAATAAGAAAAGCAAGAAGTGTACAATGTTCAGATTTGCATATTACAGCAGGAGATTTTGTAGC
AGTAAGAAGATTTGGAGAACTTAAACTGATTGATATTAAGCCAACGATTGAAGAAACAGAAAAAATGCTGTTTGATATTC
TTAATGATGAGGATATAAGAAGGGTTAAGAATGGAGAAGATATTGATGTTGCTGCATCTGACGAGTCAGGTGGACGATTA
AGAATTAATATCTATCACCAGAGAAGAAATCTTGCAGCCAGTATCCGTTTACTGGATGCAAATATACCATCATTTGAGGA
TCTTGGACTTCCAGGAAAGTTGCTTGCAGAGCTAGCATCTAAGAGAAGTGGTCTTGTTCTTGTAACAGGTCCTACAGGTA
GTGGTAAGTCTACAACACTTGCTGCAATGATTGATTATATTAATAATAATATGGCAAGACATATTATTACAGTAGAGGAT
CCTATCGAGTATGTATATGGTTTTAAGCAGTCTATGATTCATCAGAGACAGGTTGGAAGAGATGTCCCTAATTTTGCATC
TGCACTTCGTTCAGCACTTCGAGAAGATCCTGATGTAATCCTTGTAGGTGAAATGAGAGATTATGAGACAATCAATGCGG
CTATTACTGCTGCTGAGACAGGACATCTTGTAATGTCAACATTGCATACTAAGAGTGCAGCACAGACCGTTGAAAGAATT
ATCAGCGCATGCCCGATAGAGGCACAGCCTGCTCTTACAATCCAGCTTTCTTCAATTCTTACGGGTGTTATTACACAGAC
ACTTGTACCTTTTGCAAGAAAGGAAGGCCGAATTGCGGCAACAGAGGTTATGCTTAATAATACGGCTGTAGCCAACCTTA
TTAAGGAAAAGAAAACTAATCAGATTCAGACCGTTTTACAGTCAAATCTTTCATCAGGTATGCATACATTGAATTATTCG
CTTGCTAATCTTGTTAAGACAAGGGTTATTTCAAGAGAAGCTGCTCTTAAACATTCAGAGAACCCAGCTGATCTTGCAAA
GAATATATAA

Upstream 100 bases:

>100_bases
GCAAGAAAAATGGGATAAAAGTATGATTGAATACGTATTTTTATTGTGGTAGTATCTTATTGTTGTTCATACTAATGAAC
AAAATGTATGGGAGGGGAAC

Downstream 100 bases:

>100_bases
TTGATATAATAGTAAAGAAAAAATATTTGAAAATGAAGCCAATCTACTGTATTATGGAGTCGAAAGTATTACGGACAAAT
CTATAATACAGTCGAAAGGC

Product: twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MELKQLIRKARSVQCSDLHITAGDFVAVRRFGELKLIDIKPTIEETEKMLFDILNDEDIRRVKNGEDIDVAASDESGGRL
RINIYHQRRNLAASIRLLDANIPSFEDLGLPGKLLAELASKRSGLVLVTGPTGSGKSTTLAAMIDYINNNMARHIITVED
PIEYVYGFKQSMIHQRQVGRDVPNFASALRSALREDPDVILVGEMRDYETINAAITAAETGHLVMSTLHTKSAAQTVERI
ISACPIEAQPALTIQLSSILTGVITQTLVPFARKEGRIAATEVMLNNTAVANLIKEKKTNQIQTVLQSNLSSGMHTLNYS
LANLVKTRVISREAALKHSENPADLAKNI

Sequences:

>Translated_349_residues
MELKQLIRKARSVQCSDLHITAGDFVAVRRFGELKLIDIKPTIEETEKMLFDILNDEDIRRVKNGEDIDVAASDESGGRL
RINIYHQRRNLAASIRLLDANIPSFEDLGLPGKLLAELASKRSGLVLVTGPTGSGKSTTLAAMIDYINNNMARHIITVED
PIEYVYGFKQSMIHQRQVGRDVPNFASALRSALREDPDVILVGEMRDYETINAAITAAETGHLVMSTLHTKSAAQTVERI
ISACPIEAQPALTIQLSSILTGVITQTLVPFARKEGRIAATEVMLNNTAVANLIKEKKTNQIQTVLQSNLSSGMHTLNYS
LANLVKTRVISREAALKHSENPADLAKNI
>Mature_349_residues
MELKQLIRKARSVQCSDLHITAGDFVAVRRFGELKLIDIKPTIEETEKMLFDILNDEDIRRVKNGEDIDVAASDESGGRL
RINIYHQRRNLAASIRLLDANIPSFEDLGLPGKLLAELASKRSGLVLVTGPTGSGKSTTLAAMIDYINNNMARHIITVED
PIEYVYGFKQSMIHQRQVGRDVPNFASALRSALREDPDVILVGEMRDYETINAAITAAETGHLVMSTLHTKSAAQTVERI
ISACPIEAQPALTIQLSSILTGVITQTLVPFARKEGRIAATEVMLNNTAVANLIKEKKTNQIQTVLQSNLSSGMHTLNYS
LANLVKTRVISREAALKHSENPADLAKNI

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=326, Percent_Identity=42.0245398773006, Blast_Score=248, Evalue=3e-67,
Organism=Escherichia coli, GI1789723, Length=274, Percent_Identity=38.3211678832117, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1786296, Length=291, Percent_Identity=35.7388316151203, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38375; Mature: 38375

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKQLIRKARSVQCSDLHITAGDFVAVRRFGELKLIDIKPTIEETEKMLFDILNDEDIR
CCHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCHHHH
RVKNGEDIDVAASDESGGRLRINIYHQRRNLAASIRLLDANIPSFEDLGLPGKLLAELAS
HHCCCCCEEEEECCCCCCEEEEEEEECCCCHHHEEEEEECCCCCHHHCCCCHHHHHHHHH
KRSGLVLVTGPTGSGKSTTLAAMIDYINNNMARHIITVEDPIEYVYGFKQSMIHQRQVGR
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCC
DVPNFASALRSALREDPDVILVGEMRDYETINAAITAAETGHLVMSTLHTKSAAQTVERI
CCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHH
ISACPIEAQPALTIQLSSILTGVITQTLVPFARKEGRIAATEVMLNNTAVANLIKEKKTN
HHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEHHEECCCHHHHHHHHHHHHH
QIQTVLQSNLSSGMHTLNYSLANLVKTRVISREAALKHSENPADLAKNI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure
MELKQLIRKARSVQCSDLHITAGDFVAVRRFGELKLIDIKPTIEETEKMLFDILNDEDIR
CCHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCHHHH
RVKNGEDIDVAASDESGGRLRINIYHQRRNLAASIRLLDANIPSFEDLGLPGKLLAELAS
HHCCCCCEEEEECCCCCCEEEEEEEECCCCHHHEEEEEECCCCCHHHCCCCHHHHHHHHH
KRSGLVLVTGPTGSGKSTTLAAMIDYINNNMARHIITVEDPIEYVYGFKQSMIHQRQVGR
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCC
DVPNFASALRSALREDPDVILVGEMRDYETINAAITAAETGHLVMSTLHTKSAAQTVERI
CCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHH
ISACPIEAQPALTIQLSSILTGVITQTLVPFARKEGRIAATEVMLNNTAVANLIKEKKTN
HHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEHHEECCCHHHHHHHHHHHHH
QIQTVLQSNLSSGMHTLNYSLANLVKTRVISREAALKHSENPADLAKNI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]