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The map label for this gene is rhgT [H]

Identifier: 238917337

GI number: 238917337

Start: 1462134

End: 1463564

Strand: Reverse

Name: rhgT [H]

Synonym: EUBELI_01414

Alternate gene names: 238917337

Gene position: 1463564-1462134 (Counterclockwise)

Preceding gene: 238917338

Following gene: 238917336

Centisome position: 68.26

GC content: 40.11

Gene sequence:

>1431_bases
ATGAGTACATTATGGGTTGTTGGTGATTCAACATTAAGTTCTTTTGATGACAAATACTATTATCCAAGATATGGATACGG
AACAAAGCTTGGCTGTTATCTTAACAGCATGGTGCAGGTTAATAATCTTGCACTTTCAGGAAGAAGCAGCTTAAGCTTTA
CTAAGGAAGAGAATTATAAGGAACTTCTTGCAGGCATGAAAGCAGGAGATTTTCTTATAATCGGATTCGGACATAATGAT
GAGAAAACAGAGACAGGAAGATATACAAGCCCGATAGGCGGCAGGGATAAGAAAGGCACATTTGCAGCATCATTATATGA
TAATTATATAAAGCCGGCACTTGATGTTTCATGTACACCAATACTGTGTACGCCTATTGTAAGAAGAACTGCAACAGGAG
AGTGGACAAAGCAGGAGCTTCACATAACTGATGACGCTGCACAGTTTAAGGGAGGGGATTATTCACAGGCAGTCCGTGAC
CTTGCAAGAGACCTTGGAATCGTCTGTGTTGATATGACCGAAAAGACAAAGGCTTTATATGATGAGTTAGGACCAGAAGA
GACAATTTATCTTCACGCATGGCCATCTAATAAAGAAGTAAGTGTTGACAATACACACACTAATATATGGGGCGGCAGGG
TTAATGCGTTCCTTGTTATGCAGGAGCTTGAAAAGGCTGGAATATCAGGACTTTCAGAGAATATAGTTAATATAAGGGCA
GATGAACCGCTTCCAGACAAGAACAGATATCTTGAAAAAAATGCTTCATACAAGCCGGTAGAGTTTAGCGATGAGCTTGC
TGACAGCAAGAACTTTAAAGATGCATATGGATTCAAAGGCACAGTATTCGGGGATGTTACAACTCTGCCAACGGAATCTG
ATAATTATATACTTGAAGAAGTTCCAGGGGGAATACACATCGCGGTTAAGAATAATGATGGCAAGATATCTGCTGTTACA
GACGGAATTGCAATGTACTATAAGAAGATTCCTGTAAATGTGAACTTTACCCTTAAGGCTAAGATGACAATTAATGATTA
TTTCTATAACAATCAGGTGTCATTTGGGCTTATGGTAAGAGATGACATGTATATTGATAAGAAAATGCCCGATGTACTTG
GCGATTATGTAGCGGCAGCACCGCTTAACCTTACTTATAAAGACCAGGCATGGAGTTGTTTTGCAAGAAAGAACAGCGAG
CTTATGCAGGGAAGCGTGACAGGAAGAGAGCTTAAGCCGGGTGATACCGTAGAAGTATGTATAAAGTCTAATCCGGACGG
CTATGCAGTAAAATTAGGCGATGGAGAGTTCTTAACAGGCGGATTTGATTTTAAGCTGACAGCCGTAGACCCTAAGCATG
TATACGCAGGATTCTTCGTTTCGAGAAATGCGGATGTTACATTTACGGATATAGAATATACAGAGAATTAA

Upstream 100 bases:

>100_bases
TCGAGAAAGAGATAGCTGCGATGGAAGAACACCTCCGCGAAAACTACCTTAATAAAACATATTGATGATAACACATATAT
TTAAAACGAGGTGACATACG

Downstream 100 bases:

>100_bases
TACAATTTTTATATATGATTGTATTTAATTTTACATAGGAAAATGTTACAATTACATCAGAAATATCGTGAAAAAATAGC
GTTGACGCGGAAATGAGGCA

Product: carbohydrate esterase family 12 protein

Products: NA

Alternate protein names: RGAE [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSTLWVVGDSTLSSFDDKYYYPRYGYGTKLGCYLNSMVQVNNLALSGRSSLSFTKEENYKELLAGMKAGDFLIIGFGHND
EKTETGRYTSPIGGRDKKGTFAASLYDNYIKPALDVSCTPILCTPIVRRTATGEWTKQELHITDDAAQFKGGDYSQAVRD
LARDLGIVCVDMTEKTKALYDELGPEETIYLHAWPSNKEVSVDNTHTNIWGGRVNAFLVMQELEKAGISGLSENIVNIRA
DEPLPDKNRYLEKNASYKPVEFSDELADSKNFKDAYGFKGTVFGDVTTLPTESDNYILEEVPGGIHIAVKNNDGKISAVT
DGIAMYYKKIPVNVNFTLKAKMTINDYFYNNQVSFGLMVRDDMYIDKKMPDVLGDYVAAAPLNLTYKDQAWSCFARKNSE
LMQGSVTGRELKPGDTVEVCIKSNPDGYAVKLGDGEFLTGGFDFKLTAVDPKHVYAGFFVSRNADVTFTDIEYTEN

Sequences:

>Translated_476_residues
MSTLWVVGDSTLSSFDDKYYYPRYGYGTKLGCYLNSMVQVNNLALSGRSSLSFTKEENYKELLAGMKAGDFLIIGFGHND
EKTETGRYTSPIGGRDKKGTFAASLYDNYIKPALDVSCTPILCTPIVRRTATGEWTKQELHITDDAAQFKGGDYSQAVRD
LARDLGIVCVDMTEKTKALYDELGPEETIYLHAWPSNKEVSVDNTHTNIWGGRVNAFLVMQELEKAGISGLSENIVNIRA
DEPLPDKNRYLEKNASYKPVEFSDELADSKNFKDAYGFKGTVFGDVTTLPTESDNYILEEVPGGIHIAVKNNDGKISAVT
DGIAMYYKKIPVNVNFTLKAKMTINDYFYNNQVSFGLMVRDDMYIDKKMPDVLGDYVAAAPLNLTYKDQAWSCFARKNSE
LMQGSVTGRELKPGDTVEVCIKSNPDGYAVKLGDGEFLTGGFDFKLTAVDPKHVYAGFFVSRNADVTFTDIEYTEN
>Mature_475_residues
STLWVVGDSTLSSFDDKYYYPRYGYGTKLGCYLNSMVQVNNLALSGRSSLSFTKEENYKELLAGMKAGDFLIIGFGHNDE
KTETGRYTSPIGGRDKKGTFAASLYDNYIKPALDVSCTPILCTPIVRRTATGEWTKQELHITDDAAQFKGGDYSQAVRDL
ARDLGIVCVDMTEKTKALYDELGPEETIYLHAWPSNKEVSVDNTHTNIWGGRVNAFLVMQELEKAGISGLSENIVNIRAD
EPLPDKNRYLEKNASYKPVEFSDELADSKNFKDAYGFKGTVFGDVTTLPTESDNYILEEVPGGIHIAVKNNDGKISAVTD
GIAMYYKKIPVNVNFTLKAKMTINDYFYNNQVSFGLMVRDDMYIDKKMPDVLGDYVAAAPLNLTYKDQAWSCFARKNSEL
MQGSVTGRELKPGDTVEVCIKSNPDGYAVKLGDGEFLTGGFDFKLTAVDPKHVYAGFFVSRNADVTFTDIEYTEN

Specific function: May play role in the degradation of type I rhamnogalacturonan derived from plant cell walls. This enzyme has a broad substrate specificity, and shows strong preference for glucose pentaacetate, beta-naphthylacetate, and p-nitrophenyl acetate (pNPA). Also

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: NA

Molecular weight: Translated: 52952; Mature: 52821

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLWVVGDSTLSSFDDKYYYPRYGYGTKLGCYLNSMVQVNNLALSGRSSLSFTKEENYK
CCEEEEEECCHHHHCCCCCCCCCCCCCCCHHHHHHHHEEEEEEEECCCCCEEECHHCCHH
ELLAGMKAGDFLIIGFGHNDEKTETGRYTSPIGGRDKKGTFAASLYDNYIKPALDVSCTP
HHHHCCCCCCEEEEEECCCCCCCCCCCEECCCCCCCCCCEEEEHHHHHHCCCCCCCCCCC
ILCTPIVRRTATGEWTKQELHITDDAAQFKGGDYSQAVRDLARDLGIVCVDMTEKTKALY
CHHHHHHHHCCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHH
DELGPEETIYLHAWPSNKEVSVDNTHTNIWGGRVNAFLVMQELEKAGISGLSENIVNIRA
HHCCCCCEEEEEECCCCCEEEECCCCCEECCCCEEEEHHHHHHHHHCCCCCCCCEEEEEC
DEPLPDKNRYLEKNASYKPVEFSDELADSKNFKDAYGFKGTVFGDVTTLPTESDNYILEE
CCCCCCCCHHHHCCCCCCCCCCCHHHCCCCCCCHHCCCCEEEEECEEECCCCCCCEEHEE
VPGGIHIAVKNNDGKISAVTDGIAMYYKKIPVNVNFTLKAKMTINDYFYNNQVSFGLMVR
CCCCEEEEEECCCCEEEEEECCCEEEEEECCEEEEEEEEEEEEEEEEEECCEEEEEEEEE
DDMYIDKKMPDVLGDYVAAAPLNLTYKDQAWSCFARKNSELMQGSVTGRELKPGDTVEVC
CCEEECCCCHHHHHHHHHHCCCCEEECCCCCEEECCCCCHHHCCCCCCCCCCCCCEEEEE
IKSNPDGYAVKLGDGEFLTGGFDFKLTAVDPKHVYAGFFVSRNADVTFTDIEYTEN
EECCCCCEEEEECCCCEEECCCEEEEEEECCCCEEEEEEEECCCCEEEEEEEECCC
>Mature Secondary Structure 
STLWVVGDSTLSSFDDKYYYPRYGYGTKLGCYLNSMVQVNNLALSGRSSLSFTKEENYK
CEEEEEECCHHHHCCCCCCCCCCCCCCCHHHHHHHHEEEEEEEECCCCCEEECHHCCHH
ELLAGMKAGDFLIIGFGHNDEKTETGRYTSPIGGRDKKGTFAASLYDNYIKPALDVSCTP
HHHHCCCCCCEEEEEECCCCCCCCCCCEECCCCCCCCCCEEEEHHHHHHCCCCCCCCCCC
ILCTPIVRRTATGEWTKQELHITDDAAQFKGGDYSQAVRDLARDLGIVCVDMTEKTKALY
CHHHHHHHHCCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHH
DELGPEETIYLHAWPSNKEVSVDNTHTNIWGGRVNAFLVMQELEKAGISGLSENIVNIRA
HHCCCCCEEEEEECCCCCEEEECCCCCEECCCCEEEEHHHHHHHHHCCCCCCCCEEEEEC
DEPLPDKNRYLEKNASYKPVEFSDELADSKNFKDAYGFKGTVFGDVTTLPTESDNYILEE
CCCCCCCCHHHHCCCCCCCCCCCHHHCCCCCCCHHCCCCEEEEECEEECCCCCCCEEHEE
VPGGIHIAVKNNDGKISAVTDGIAMYYKKIPVNVNFTLKAKMTINDYFYNNQVSFGLMVR
CCCCEEEEEECCCCEEEEEECCCEEEEEECCEEEEEEEEEEEEEEEEEECCEEEEEEEEE
DDMYIDKKMPDVLGDYVAAAPLNLTYKDQAWSCFARKNSELMQGSVTGRELKPGDTVEVC
CCEEECCCCHHHHHHHHHHCCCCEEECCCCCEEECCCCCHHHCCCCCCCCCCCCCEEEEE
IKSNPDGYAVKLGDGEFLTGGFDFKLTAVDPKHVYAGFFVSRNADVTFTDIEYTEN
EECCCCCEEEEECCCCEEECCCEEEEEEECCCCEEEEEEEECCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]