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The map label for this gene is gph [C]

Identifier: 238917213

GI number: 238917213

Start: 1323930

End: 1324616

Strand: Reverse

Name: gph [C]

Synonym: EUBELI_01286

Alternate gene names: 238917213

Gene position: 1324616-1323930 (Counterclockwise)

Preceding gene: 238917214

Following gene: 238917212

Centisome position: 61.78

GC content: 36.39

Gene sequence:

>687_bases
ATGACTAAGAGATATATATTATTTGACCTTGATGGAACAATTACAGATTCAAGCGAGGGAATTACCAAAAGTGTGCAGCA
TGCACTTAAGAAACTTGGAATAGAAGAAAATGACCAGGCGATGCTTCGAAGATTCATAGGTCCTCCTCTTGATGAAAGCT
TTGAAAAATTTTATGGATTTGACAAAGAAAAAGCACTAAAGGCAGTTGATTATTACCGTGAAAGATATTCTGACAAGGGA
ATATATGAAAATGTTCTTTTTGACGGGATAGCAGATATGCTTGAAGGATTAAAGAGTGATGGATATATAATTGCACTTGC
TACATGTAAGCCTGAAATATATGTTCCAAGAATATTAGAGTACTTTAATGTTGATAAGTATTTTGATGTTGCTGTTGGAA
GTGAATTAGAAGGTGGAGAACGTAGACATAAGAACCAGGTTATTGATGAAGTGTTTGTAAGGCTTGCTGCTGCCGGACTT
GCGGACGCTGACAATCTGCCAGAAACAAAGTCACAGTCTATTATGATTGGTGACAGAAAAGACGATATATTAGGTGCTAA
AGCTTCTGGAATTGATTCTATGGGAGTAAGATATGGTTTTGCAGAAGAACAGGAGCTTGAGATAGCGGGAGCAGATTATA
TTGTTGAAACTGTAGAGGACATAAGGAAAAAACTAAAGACACTATAA

Upstream 100 bases:

>100_bases
GTATATGATGAGGCTTCAGCGGATGACTGGCAGGATATTTGTCAGATAGCCGATTACAATATCAGAAGTTACAGTGAATT
AATGTGATAATGGAGAAAAT

Downstream 100 bases:

>100_bases
TCGTTTCTAAGATTAATAATATACGGGAGGAAAACCCAATGAAAAAATTCATTCAGGAATTTAAAGAATTTGCTTTGAAA
GGAAATGTTATGAATCTGGC

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: Nucleoside 5'-monophosphate phosphohydrolase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTKRYILFDLDGTITDSSEGITKSVQHALKKLGIEENDQAMLRRFIGPPLDESFEKFYGFDKEKALKAVDYYRERYSDKG
IYENVLFDGIADMLEGLKSDGYIIALATCKPEIYVPRILEYFNVDKYFDVAVGSELEGGERRHKNQVIDEVFVRLAAAGL
ADADNLPETKSQSIMIGDRKDDILGAKASGIDSMGVRYGFAEEQELEIAGADYIVETVEDIRKKLKTL

Sequences:

>Translated_228_residues
MTKRYILFDLDGTITDSSEGITKSVQHALKKLGIEENDQAMLRRFIGPPLDESFEKFYGFDKEKALKAVDYYRERYSDKG
IYENVLFDGIADMLEGLKSDGYIIALATCKPEIYVPRILEYFNVDKYFDVAVGSELEGGERRHKNQVIDEVFVRLAAAGL
ADADNLPETKSQSIMIGDRKDDILGAKASGIDSMGVRYGFAEEQELEIAGADYIVETVEDIRKKLKTL
>Mature_227_residues
TKRYILFDLDGTITDSSEGITKSVQHALKKLGIEENDQAMLRRFIGPPLDESFEKFYGFDKEKALKAVDYYRERYSDKGI
YENVLFDGIADMLEGLKSDGYIIALATCKPEIYVPRILEYFNVDKYFDVAVGSELEGGERRHKNQVIDEVFVRLAAAGLA
DADNLPETKSQSIMIGDRKDDILGAKASGIDSMGVRYGFAEEQELEIAGADYIVETVEDIRKKLKTL

Specific function: Specifically dephosphorylates nucleoside 5'- monophosphates to nucleosides and inorganic phosphate. Displays high activity toward 5'-UMP and 5'-IMP, significant activity against 5'-XMP and 5'-TMP, and low activity against 5'-CMP [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=250, Percent_Identity=26.4, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 25692; Mature: 25561

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRYILFDLDGTITDSSEGITKSVQHALKKLGIEENDQAMLRRFIGPPLDESFEKFYGF
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHCC
DKEKALKAVDYYRERYSDKGIYENVLFDGIADMLEGLKSDGYIIALATCKPEIYVPRILE
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
YFNVDKYFDVAVGSELEGGERRHKNQVIDEVFVRLAAAGLADADNLPETKSQSIMIGDRK
HHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCC
DDILGAKASGIDSMGVRYGFAEEQELEIAGADYIVETVEDIRKKLKTL
CCCCCCCCCCCCHHHHCCCCCCCCCEEHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKRYILFDLDGTITDSSEGITKSVQHALKKLGIEENDQAMLRRFIGPPLDESFEKFYGF
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHCC
DKEKALKAVDYYRERYSDKGIYENVLFDGIADMLEGLKSDGYIIALATCKPEIYVPRILE
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
YFNVDKYFDVAVGSELEGGERRHKNQVIDEVFVRLAAAGLADADNLPETKSQSIMIGDRK
HHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCC
DDILGAKASGIDSMGVRYGFAEEQELEIAGADYIVETVEDIRKKLKTL
CCCCCCCCCCCCHHHHCCCCCCCCCEEHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]