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The map label for this gene is mscL [H]

Identifier: 238917212

GI number: 238917212

Start: 1323463

End: 1323891

Strand: Reverse

Name: mscL [H]

Synonym: EUBELI_01285

Alternate gene names: 238917212

Gene position: 1323891-1323463 (Counterclockwise)

Preceding gene: 238917213

Following gene: 238917211

Centisome position: 61.74

GC content: 35.2

Gene sequence:

>429_bases
ATGAAAAAATTCATTCAGGAATTTAAAGAATTTGCTTTGAAAGGAAATGTTATGAATCTGGCAGTAGGTGTTATTATCGG
TGCTGCATTTCAGAGCATTGTAACAGCACTTACAAGCAGTTTTATTAATCCGCTTATTGCCGTAGTTACAGGTGGTACTA
ATGGTGAGGGTGTTACCGTTGGTGGTACATTTACAATAAGAGGAGTTGTGTTTGATTATGGTTCATTCATAACAGCAGTT
ATTAATTTCCTTATTATGGCATTTGTTCTGTTCCTTTTAGTTAAAGCAATGAACAAGATGATGAGCTTAGGTAAGAAGAA
AGAAGAACCGGCTCCAACTACAAAGGATTGTCCATACTGTTTCAGCAAGATATCTGTTAAGGCAACAAGATGTCCTTTCT
GTACAACTGAATTAGTAGATAAGAAATAA

Upstream 100 bases:

>100_bases
CGGGAGCAGATTATATTGTTGAAACTGTAGAGGACATAAGGAAAAAACTAAAGACACTATAATCGTTTCTAAGATTAATA
ATATACGGGAGGAAAACCCA

Downstream 100 bases:

>100_bases
AATAAAGCCATATATGCAGGTCTCCCAACTGCATATATGGCTTTATTCATGTTTTTCATAAATGGATGTATCCCAAACCT
GTGTTCACGTCCAATTCACA

Product: MscL family large conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MKKFIQEFKEFALKGNVMNLAVGVIIGAAFQSIVTALTSSFINPLIAVVTGGTNGEGVTVGGTFTIRGVVFDYGSFITAV
INFLIMAFVLFLLVKAMNKMMSLGKKKEEPAPTTKDCPYCFSKISVKATRCPFCTTELVDKK

Sequences:

>Translated_142_residues
MKKFIQEFKEFALKGNVMNLAVGVIIGAAFQSIVTALTSSFINPLIAVVTGGTNGEGVTVGGTFTIRGVVFDYGSFITAV
INFLIMAFVLFLLVKAMNKMMSLGKKKEEPAPTTKDCPYCFSKISVKATRCPFCTTELVDKK
>Mature_142_residues
MKKFIQEFKEFALKGNVMNLAVGVIIGAAFQSIVTALTSSFINPLIAVVTGGTNGEGVTVGGTFTIRGVVFDYGSFITAV
INFLIMAFVLFLLVKAMNKMMSLGKKKEEPAPTTKDCPYCFSKISVKATRCPFCTTELVDKK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=120, Percent_Identity=45, Blast_Score=93, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 15365; Mature: 15365

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFIQEFKEFALKGNVMNLAVGVIIGAAFQSIVTALTSSFINPLIAVVTGGTNGEGVTV
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
GGTFTIRGVVFDYGSFITAVINFLIMAFVLFLLVKAMNKMMSLGKKKEEPAPTTKDCPYC
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
FSKISVKATRCPFCTTELVDKK
HHHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure
MKKFIQEFKEFALKGNVMNLAVGVIIGAAFQSIVTALTSSFINPLIAVVTGGTNGEGVTV
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
GGTFTIRGVVFDYGSFITAVINFLIMAFVLFLLVKAMNKMMSLGKKKEEPAPTTKDCPYC
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
FSKISVKATRCPFCTTELVDKK
HHHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA