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The map label for this gene is yitJ [H]

Identifier: 238917210

GI number: 238917210

Start: 1319938

End: 1321725

Strand: Reverse

Name: yitJ [H]

Synonym: EUBELI_01283

Alternate gene names: 238917210

Gene position: 1321725-1319938 (Counterclockwise)

Preceding gene: 238917211

Following gene: 238917209

Centisome position: 61.64

GC content: 37.64

Gene sequence:

>1788_bases
ATGAAGTATACAGGAACAGCAGGAATATTATATGACAGATTAAAGAGTGAAATACTGGTGGCAGATGGTGCGTTTGGAAC
ATACTATGCTAAGAAGTATGATACGGGCAGCCTGCCTGAGCTGGCAAATTTACAGGCTTCAGACAGAGTACTGGCAATTC
ATAAAGAATATATTGAAGCTGGTGCTAAGATTATCAGAACTAATACATTTGCAAGTAATACCGTGTGTCTTGGAGCGGGA
TTTGATGAGGTAAGAAATAATATTAAAAATGCCGTTGACATTGCAAGGGAAGCCGTAAAGGGGACAGATGTGCTTGTAGC
AGCTGATATTGGCCCTATTCCTGGTGAAAATATGATTGAAAAAGAGCATATTGAAAAAGAGTATGTTGATATAGTTAATG
TTTTTAAGGAATGTGGCGTTGATATATTTGTTTTTGAAACATTTCCAGAACTGGGATTTATTGATAAAGCAATTGAAAAT
GCAGGAGAAAACGGATTTGTTATAACTCAGTTTGCAATAAATCAGTTTGGCTATAGCAGTGCAGGCTTTAGTGCAAGAAA
GCTGATTGAAGAAGCTGGGAAAAATTCATATATTGATGCATGTGGATTTAACTGCGGAGTTGGTCCGGGACATATGAAGA
AGCTTGTGCAGAGCCTTATTAACAGGAATGACAAGTATTTTGCGGTGCTTCCAAATGCAGGATATCCACAGGTTGTAAGC
GGTCGAATGGTATTTGATGATAACAATGCAGCATATTTTTCACAGATTATGCATGATCTGGCTGAGGATGGAGCTGATAT
TATTGGCGGCTGTTGTGGAACAACACCTGAATATATAAGACAGATGGTTTTAAAAACTGCTGATGTTGTTCATATGAAAA
ATGCTTCCATAGAAGAAGAGATATCAGAAAAGAAAACTGCAAATGATGTTTCTTTCTATAAAGGAAAAGAAGGAAGAAAA
CTGATTGCTGTAGAACTTGCTCCTCCGCTTGGAATTGATGATGAGAAGATTATGGATGCTGCTTTTTTGATGAAGGAAAG
CGGAGTTGATGTGCTTACATTTCCGGATTCACCATCAGGAAGAACACGTGCAGATTCAATACTTATGGCAGAAAAGGTTT
CGAGAGAAACGGGAATGTGTGTTATGCCGCACATATGCTGTCGTGACAAGAATGCTATAGCAATGCGTTCACAGCTTTTG
GGAGCACATATAAATGATATTAATAATTTTCTCGTTATTACCGGTGATCCCATACCATCAGTTGTAAGAGCGTCTGTAAA
GAGTGTGTTTAACTTCGATTCAGTTGGACTTATGAATATAATAAGTGATATGAATCAGGAACAGTTTGCCGGGAAACCGG
TTATATATGGTGGAGCAATTAATCAGGGAAGGGTTAATTTTAAAGTGGAACTTGAGCGTGTTAAGAAAAAAATGGAGGCG
GGAGCCACATTTTTTATGACGCAGCCTGTATTTTCTGATGAAGATATAGACAGACTCAGACAGATTAAAGAACAGACAGG
GGCAAGGATTCTTTGTGGAATAATGCCATTTGTAAGTCTTCGAAATGCAACATTCATGAAAAATGAGATGACAGGTATTA
ATGTTACTGATGAGATTCTTTCAAGATACAGAGCTGATATGTCAAAAGAAGAGGGAGAAGAGACCGGTATTCAGATAGCA
AGGGAAATAATTGCAAAGACTACTGATTTTGTTGATGGGTATTATTTTTCATTTCCATTTAACAGGGTACATATGTTAAA
GAAGATTATTGGGAAGAACAATCAGTAA

Upstream 100 bases:

>100_bases
TTTTAGTGCAAATTAAACTTAATAATGATACAATTTTGGAGTCACTTATATAAGGTGGCTCCTTTTTTAAGTTGAATGTT
AAAAGATAAGGGGACTGGGC

Downstream 100 bases:

>100_bases
GTAGGACTATTCTATTGACTTATTAGAAATGTATCTGATATAATGTTGAAAAGTTTCGATGCGGAATAATTTAATAATAT
GTGAGGTGTGAGGTGTGGTA

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MKYTGTAGILYDRLKSEILVADGAFGTYYAKKYDTGSLPELANLQASDRVLAIHKEYIEAGAKIIRTNTFASNTVCLGAG
FDEVRNNIKNAVDIAREAVKGTDVLVAADIGPIPGENMIEKEHIEKEYVDIVNVFKECGVDIFVFETFPELGFIDKAIEN
AGENGFVITQFAINQFGYSSAGFSARKLIEEAGKNSYIDACGFNCGVGPGHMKKLVQSLINRNDKYFAVLPNAGYPQVVS
GRMVFDDNNAAYFSQIMHDLAEDGADIIGGCCGTTPEYIRQMVLKTADVVHMKNASIEEEISEKKTANDVSFYKGKEGRK
LIAVELAPPLGIDDEKIMDAAFLMKESGVDVLTFPDSPSGRTRADSILMAEKVSRETGMCVMPHICCRDKNAIAMRSQLL
GAHINDINNFLVITGDPIPSVVRASVKSVFNFDSVGLMNIISDMNQEQFAGKPVIYGGAINQGRVNFKVELERVKKKMEA
GATFFMTQPVFSDEDIDRLRQIKEQTGARILCGIMPFVSLRNATFMKNEMTGINVTDEILSRYRADMSKEEGEETGIQIA
REIIAKTTDFVDGYYFSFPFNRVHMLKKIIGKNNQ

Sequences:

>Translated_595_residues
MKYTGTAGILYDRLKSEILVADGAFGTYYAKKYDTGSLPELANLQASDRVLAIHKEYIEAGAKIIRTNTFASNTVCLGAG
FDEVRNNIKNAVDIAREAVKGTDVLVAADIGPIPGENMIEKEHIEKEYVDIVNVFKECGVDIFVFETFPELGFIDKAIEN
AGENGFVITQFAINQFGYSSAGFSARKLIEEAGKNSYIDACGFNCGVGPGHMKKLVQSLINRNDKYFAVLPNAGYPQVVS
GRMVFDDNNAAYFSQIMHDLAEDGADIIGGCCGTTPEYIRQMVLKTADVVHMKNASIEEEISEKKTANDVSFYKGKEGRK
LIAVELAPPLGIDDEKIMDAAFLMKESGVDVLTFPDSPSGRTRADSILMAEKVSRETGMCVMPHICCRDKNAIAMRSQLL
GAHINDINNFLVITGDPIPSVVRASVKSVFNFDSVGLMNIISDMNQEQFAGKPVIYGGAINQGRVNFKVELERVKKKMEA
GATFFMTQPVFSDEDIDRLRQIKEQTGARILCGIMPFVSLRNATFMKNEMTGINVTDEILSRYRADMSKEEGEETGIQIA
REIIAKTTDFVDGYYFSFPFNRVHMLKKIIGKNNQ
>Mature_595_residues
MKYTGTAGILYDRLKSEILVADGAFGTYYAKKYDTGSLPELANLQASDRVLAIHKEYIEAGAKIIRTNTFASNTVCLGAG
FDEVRNNIKNAVDIAREAVKGTDVLVAADIGPIPGENMIEKEHIEKEYVDIVNVFKECGVDIFVFETFPELGFIDKAIEN
AGENGFVITQFAINQFGYSSAGFSARKLIEEAGKNSYIDACGFNCGVGPGHMKKLVQSLINRNDKYFAVLPNAGYPQVVS
GRMVFDDNNAAYFSQIMHDLAEDGADIIGGCCGTTPEYIRQMVLKTADVVHMKNASIEEEISEKKTANDVSFYKGKEGRK
LIAVELAPPLGIDDEKIMDAAFLMKESGVDVLTFPDSPSGRTRADSILMAEKVSRETGMCVMPHICCRDKNAIAMRSQLL
GAHINDINNFLVITGDPIPSVVRASVKSVFNFDSVGLMNIISDMNQEQFAGKPVIYGGAINQGRVNFKVELERVKKKMEA
GATFFMTQPVFSDEDIDRLRQIKEQTGARILCGIMPFVSLRNATFMKNEMTGINVTDEILSRYRADMSKEEGEETGIQIA
REIIAKTTDFVDGYYFSFPFNRVHMLKKIIGKNNQ

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=315, Percent_Identity=24.1269841269841, Blast_Score=85, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535405, Length=317, Percent_Identity=25.2365930599369, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 65701; Mature: 65701

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTGTAGILYDRLKSEILVADGAFGTYYAKKYDTGSLPELANLQASDRVLAIHKEYIEA
CCCCCCHHHHHHHHHCCEEEECCCCCCCEECCCCCCCCCHHHCCCCCCCEEHHHHHHHHC
GAKIIRTNTFASNTVCLGAGFDEVRNNIKNAVDIAREAVKGTDVLVAADIGPIPGENMIE
CCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
KEHIEKEYVDIVNVFKECGVDIFVFETFPELGFIDKAIENAGENGFVITQFAINQFGYSS
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEEEEHHHHCCCCC
AGFSARKLIEEAGKNSYIDACGFNCGVGPGHMKKLVQSLINRNDKYFAVLPNAGYPQVVS
CCHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
GRMVFDDNNAAYFSQIMHDLAEDGADIIGGCCGTTPEYIRQMVLKTADVVHMKNASIEEE
CEEEEECCCHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHEECCCCHHHH
ISEKKTANDVSFYKGKEGRKLIAVELAPPLGIDDEKIMDAAFLMKESGVDVLTFPDSPSG
HHHHHCCCCCHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
RTRADSILMAEKVSRETGMCVMPHICCRDKNAIAMRSQLLGAHINDINNFLVITGDPIPS
CCHHHHHHHHHHHHHCCCCEEECHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHH
VVRASVKSVFNFDSVGLMNIISDMNQEQFAGKPVIYGGAINQGRVNFKVELERVKKKMEA
HHHHHHHHHHCCCCCHHHHHHHHCCHHHHCCCCEEECCCCCCCEEEEEEEHHHHHHHHHC
GATFFMTQPVFSDEDIDRLRQIKEQTGARILCGIMPFVSLRNATFMKNEMTGINVTDEIL
CCEEEEECCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCEECCCCCCCCCHHHHH
SRYRADMSKEEGEETGIQIAREIIAKTTDFVDGYYFSFPFNRVHMLKKIIGKNNQ
HHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKYTGTAGILYDRLKSEILVADGAFGTYYAKKYDTGSLPELANLQASDRVLAIHKEYIEA
CCCCCCHHHHHHHHHCCEEEECCCCCCCEECCCCCCCCCHHHCCCCCCCEEHHHHHHHHC
GAKIIRTNTFASNTVCLGAGFDEVRNNIKNAVDIAREAVKGTDVLVAADIGPIPGENMIE
CCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
KEHIEKEYVDIVNVFKECGVDIFVFETFPELGFIDKAIENAGENGFVITQFAINQFGYSS
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEEEEHHHHCCCCC
AGFSARKLIEEAGKNSYIDACGFNCGVGPGHMKKLVQSLINRNDKYFAVLPNAGYPQVVS
CCHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
GRMVFDDNNAAYFSQIMHDLAEDGADIIGGCCGTTPEYIRQMVLKTADVVHMKNASIEEE
CEEEEECCCHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHEECCCCHHHH
ISEKKTANDVSFYKGKEGRKLIAVELAPPLGIDDEKIMDAAFLMKESGVDVLTFPDSPSG
HHHHHCCCCCHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
RTRADSILMAEKVSRETGMCVMPHICCRDKNAIAMRSQLLGAHINDINNFLVITGDPIPS
CCHHHHHHHHHHHHHCCCCEEECHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHH
VVRASVKSVFNFDSVGLMNIISDMNQEQFAGKPVIYGGAINQGRVNFKVELERVKKKMEA
HHHHHHHHHHCCCCCHHHHHHHHCCHHHHCCCCEEECCCCCCCEEEEEEEHHHHHHHHHC
GATFFMTQPVFSDEDIDRLRQIKEQTGARILCGIMPFVSLRNATFMKNEMTGINVTDEIL
CCEEEEECCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCEECCCCCCCCCHHHHH
SRYRADMSKEEGEETGIQIAREIIAKTTDFVDGYYFSFPFNRVHMLKKIIGKNNQ
HHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]