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The map label for this gene is cwlM [H]

Identifier: 238917194

GI number: 238917194

Start: 1301773

End: 1302732

Strand: Direct

Name: cwlM [H]

Synonym: EUBELI_01267

Alternate gene names: 238917194

Gene position: 1301773-1302732 (Clockwise)

Preceding gene: 238917192

Following gene: 238917203

Centisome position: 60.71

GC content: 39.69

Gene sequence:

>960_bases
ATGTCTTTTGCATACAATAATATGATTAATGTTATCGGAGATATTATGGCTCAATATAAGATAGCTGTAGATGCCGGACA
CGGCGGTAGCGACTATGGTGCAACATATAATGGCAGAGCTGAGAAAGATGACAACTTAAAGCTTGCTCTTGCCGTAGGTG
ATATATTAGAAAAAAATGGTATTGACGTTGTGTACACCCGTACTACTGATGAATATGAGACACCTTTTAAAAAAGCGACT
GATGCCAATGATGCTAAGGCTGATTATTTTGTTTCAATCCACAGAAATTCATCCCCTACGCCTAACCAGTACACAGGCGT
GGAAACTCTTGTTTACAATAATTCCGGTATTAAATCACAGATGGCTGCCAATATCAATTCTGAGCTGGAAAAGGCTGGCT
TTAAAAATCTTGGAATTACCGAACGTCCTAACCTTGTTGTACTTAAACGGACAAAAATGCCTGCCGTACTTGTAGAAGCC
AGCTTTATTAACAACGATAAAGATAATGAAACATTTGATAAGAACTTTAACCAGATTGCAAATGGAATCGCAGATGGTAT
TCTTAAGACCCTTGGAATTAAACCAAAGACAAATAATACTACTGCACAGGCTGCTTCAGGTGAAGTCCCATCAGTGAAAT
CAGATTCTGCCAAATCCGCTGAAATCATGCCTGCAGCATGCGAAAAAAAGCCTGCACCTCCTGCTAAACCAGCATGTAAC
TGTGATGATGACCTCTCTCCTGAGCCACTTTATCGTGTTCAGGTTGGTGCCTACCGCAATAAAAATAATGCAGACCGCAT
GCTTAATTCCCTGCTTATTGAAGGATTCCCAGCTTTTATCATATATGAGAATGACTATTATAAAGTTCAGGTTGGTGCTT
TCAGACAGCTTTCAAACGCCATTAAAATGGAGCAGAAGCTGCGTAAATTCCGATACAACACCTACATTGTATACTCATAA

Upstream 100 bases:

>100_bases
ATATTATTTTCTGGCACTTCAAATGTATGTACATATCCAACGTTCATAATCTGTCCTCTTTCTCATCTTAAGATAAATAT
ATGTGACAGGTACGCTTATT

Downstream 100 bases:

>100_bases
TTATAAAATCGGCACCAGACTGAATATTTTCAATCTGGTGCCTGATTTTAACTATAGCAATTAGTTTTCTTCTGAATTTG
GAACTAAATTCTTCACTTCT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSFAYNNMINVIGDIMAQYKIAVDAGHGGSDYGATYNGRAEKDDNLKLALAVGDILEKNGIDVVYTRTTDEYETPFKKAT
DANDAKADYFVSIHRNSSPTPNQYTGVETLVYNNSGIKSQMAANINSELEKAGFKNLGITERPNLVVLKRTKMPAVLVEA
SFINNDKDNETFDKNFNQIANGIADGILKTLGIKPKTNNTTAQAASGEVPSVKSDSAKSAEIMPAACEKKPAPPAKPACN
CDDDLSPEPLYRVQVGAYRNKNNADRMLNSLLIEGFPAFIIYENDYYKVQVGAFRQLSNAIKMEQKLRKFRYNTYIVYS

Sequences:

>Translated_319_residues
MSFAYNNMINVIGDIMAQYKIAVDAGHGGSDYGATYNGRAEKDDNLKLALAVGDILEKNGIDVVYTRTTDEYETPFKKAT
DANDAKADYFVSIHRNSSPTPNQYTGVETLVYNNSGIKSQMAANINSELEKAGFKNLGITERPNLVVLKRTKMPAVLVEA
SFINNDKDNETFDKNFNQIANGIADGILKTLGIKPKTNNTTAQAASGEVPSVKSDSAKSAEIMPAACEKKPAPPAKPACN
CDDDLSPEPLYRVQVGAYRNKNNADRMLNSLLIEGFPAFIIYENDYYKVQVGAFRQLSNAIKMEQKLRKFRYNTYIVYS
>Mature_318_residues
SFAYNNMINVIGDIMAQYKIAVDAGHGGSDYGATYNGRAEKDDNLKLALAVGDILEKNGIDVVYTRTTDEYETPFKKATD
ANDAKADYFVSIHRNSSPTPNQYTGVETLVYNNSGIKSQMAANINSELEKAGFKNLGITERPNLVVLKRTKMPAVLVEAS
FINNDKDNETFDKNFNQIANGIADGILKTLGIKPKTNNTTAQAASGEVPSVKSDSAKSAEIMPAACEKKPAPPAKPACNC
DDDLSPEPLYRVQVGAYRNKNNADRMLNSLLIEGFPAFIIYENDYYKVQVGAFRQLSNAIKMEQKLRKFRYNTYIVYS

Specific function: Hydrolyzes the cell wall of M.luteus more efficiently than that of B.licheniformis and B.subtilis. The C-terminal region, including the repeats, determines substrate specificity [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Probable). Note=Accumulates in cells as inclusion bodies. May be secreted by a mechanism different from the normal export system [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR007730 [H]

Pfam domain/function: PF01520 Amidase_3; PF05036 SPOR [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 35179; Mature: 35048

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFAYNNMINVIGDIMAQYKIAVDAGHGGSDYGATYNGRAEKDDNLKLALAVGDILEKNG
CCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHCCC
IDVVYTRTTDEYETPFKKATDANDAKADYFVSIHRNSSPTPNQYTGVETLVYNNSGIKSQ
CEEEEEECCCCCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHH
MAANINSELEKAGFKNLGITERPNLVVLKRTKMPAVLVEASFINNDKDNETFDKNFNQIA
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEEEEEHHCCCCCCCCHHHHHHHHHH
NGIADGILKTLGIKPKTNNTTAQAASGEVPSVKSDSAKSAEIMPAACEKKPAPPAKPACN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC
CDDDLSPEPLYRVQVGAYRNKNNADRMLNSLLIEGFPAFIIYENDYYKVQVGAFRQLSNA
CCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCEEEEEHHHHHHHHHH
IKMEQKLRKFRYNTYIVYS
HHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure 
SFAYNNMINVIGDIMAQYKIAVDAGHGGSDYGATYNGRAEKDDNLKLALAVGDILEKNG
CCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHCCC
IDVVYTRTTDEYETPFKKATDANDAKADYFVSIHRNSSPTPNQYTGVETLVYNNSGIKSQ
CEEEEEECCCCCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHH
MAANINSELEKAGFKNLGITERPNLVVLKRTKMPAVLVEASFINNDKDNETFDKNFNQIA
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEEEEEHHCCCCCCCCHHHHHHHHHH
NGIADGILKTLGIKPKTNNTTAQAASGEVPSVKSDSAKSAEIMPAACEKKPAPPAKPACN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC
CDDDLSPEPLYRVQVGAYRNKNNADRMLNSLLIEGFPAFIIYENDYYKVQVGAFRQLSNA
CCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCEEEEEHHHHHHHHHH
IKMEQKLRKFRYNTYIVYS
HHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1495475 [H]