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The map label for this gene is asnB [H]

Identifier: 238917203

GI number: 238917203

Start: 1313585

End: 1315429

Strand: Direct

Name: asnB [H]

Synonym: EUBELI_01276

Alternate gene names: 238917203

Gene position: 1313585-1315429 (Clockwise)

Preceding gene: 238917194

Following gene: 238917242

Centisome position: 61.26

GC content: 38.75

Gene sequence:

>1845_bases
ATGTGTGGATTCGCTGGTTTTACCGGCTACTTAGCTGACGGTGATGCTGTTCTTGAACGCATGATGAATAAGATTATTCA
CAGAGGACCTGACAGCGCAGGTAAATACATTGATGATAAAGCGTACATGGGCTTTAGAAGACTTTCTATCATTGATCTTG
ATAACGGAAGCCAGCCTATGTTTAACGAGAATAAGAAGATAGTAATAACATTTAACGGTGAGATATATAACTATCAGGAT
TTAAGAAAAGACCTTATTGAGAAAGGTCACGTATTTGCTAACAATTCAGATACTGAAGTTCTCATTCACTCATACGAGGA
GTATGGCAAGGACATGCTTAACAAGCTTCGTGGAATGTTTGCATTCGTAATCTGGGACAGCGAGAAAGAAACTCTTTTCG
GAGCAAGAGACTTCTTCGGAATTAAGCCTTTCTACTACACAGTTGCAGACGGCAACATGATATATGGTTCAGAAATTAAA
AGTATCTTAGAGCATCCTGCTTATAAGAAAGAGGTTAATCCTGTTGCACTTGAGAATTATCTCACATTCCAGTACTCAGT
ACTTGATGAAACATTCTTCAAAGGAATATTCAAGTTGATGCCTGGTCACTTCTTTACATTCCACAAGGGTGAGCTTAACA
TTGAAAGATATTGGGAGCCTACATTTGATGCTGATGAGAGCAAGTCTTTAGATGAATTTGTTGATGAAATCGACGATGTT
ATGCATGATTCAGTTGAACACCATAAGATTAGTGATGTTGAGGTTGGTTCATTCCTCTCAAGTGGTGTTGATTCAAGCTA
TGTTGCTGCTACATTTAACGGTGACAAAACATTTACTGTTGGATTTGATTATGAAAAATACAACGAGATTGACTATGCCA
AAGCTTTATCTGACAAAATTAAAATTGACAACTATTCAAAGCTTGTTTCAAGTGAAGAATACTGGGCTGCAATTCCTAAA
ATCCAGTATCATATGGACGAGCCTTTAGCTGACCCTGCTGCGATTGCACTTTACTTTGTAAGCCAGACAGCAGCCAAGCA
TGTAAAGGTTGCCATGTCAGGTGAAGGTGCTGACGAGTTTTTTGGTGGATATAACATTTACAGAGAACCTCTCGATCTTG
CAGAATTCCAGAAGCTCCCTAAAGGCTTAAGAAAAGGACTTGCCGGCATTGCCAATGCTATTCCATTCAAGTTCAAGGGA
AAAAGCTTCTTAAACAGAGCAAGCAAGACAGTTGAAGAAAGATTCATCGGTAACGCTTTCATGTTCAATGAAAAAGAACG
TGCCAGGATATTAAAGAATCCTACCGGCAAGTATGACCACAAGCAACTTACAAAGCCTTTCTATGATAAGGTTGCTGATA
AGGATGATGTTACTAAGATGCAGTATATTGATATCAATTTCTGGCTGATAGGCGATATCTTACTTAAGGCTGATAAGATG
AGTATGGCTCACTCTCTTGAGGTAAGAGTTCCATTCCTTGATAAGGAAGTGTTCAATGTTGCCCGTACTATTCCAACTAA
GTACAAGGTTAACAAGAGTAATACCAAGTATGCCATGAGACAGGCTGCCCACAGACATCTTCCAGACATGGTTGCTGAGA
AAAAGAAGCTTGGATTCCCTGTTCCTATCAGAATCTGGCTCAAGGATGAGAAATATTACAATATGATTAAAAAGGCTTTC
ACAAGTGAAGCTGCTGAGAAATATTTTAACACTGATGAGATTGTTAAATATCTTGATGAACACAAAGAAGGAAAGGCCGA
TAATTCGCGTAAGATATGGACAATTTACATGTTCCTTGTATGGTATGAAGATTTCTTCGGCAACGGAGTTAAAGAAGCCG
CTTAA

Upstream 100 bases:

>100_bases
TGTATTAGCCTGATTACTTTGTTATAATTCATAAGTAAATTTTACATACTACATTTAACAATGCACTGATTGTGCAGACA
CAATATGGAGGTACAGATAC

Downstream 100 bases:

>100_bases
TTTTTTATAATTATAATAACAATTAACCCCTGCCGCTAATGCGACAGGGGTTTTGTTATACCTAACACATATTCTGTTAA
TTACTTATTAGTCTCTTCTA

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MCGFAGFTGYLADGDAVLERMMNKIIHRGPDSAGKYIDDKAYMGFRRLSIIDLDNGSQPMFNENKKIVITFNGEIYNYQD
LRKDLIEKGHVFANNSDTEVLIHSYEEYGKDMLNKLRGMFAFVIWDSEKETLFGARDFFGIKPFYYTVADGNMIYGSEIK
SILEHPAYKKEVNPVALENYLTFQYSVLDETFFKGIFKLMPGHFFTFHKGELNIERYWEPTFDADESKSLDEFVDEIDDV
MHDSVEHHKISDVEVGSFLSSGVDSSYVAATFNGDKTFTVGFDYEKYNEIDYAKALSDKIKIDNYSKLVSSEEYWAAIPK
IQYHMDEPLADPAAIALYFVSQTAAKHVKVAMSGEGADEFFGGYNIYREPLDLAEFQKLPKGLRKGLAGIANAIPFKFKG
KSFLNRASKTVEERFIGNAFMFNEKERARILKNPTGKYDHKQLTKPFYDKVADKDDVTKMQYIDINFWLIGDILLKADKM
SMAHSLEVRVPFLDKEVFNVARTIPTKYKVNKSNTKYAMRQAAHRHLPDMVAEKKKLGFPVPIRIWLKDEKYYNMIKKAF
TSEAAEKYFNTDEIVKYLDEHKEGKADNSRKIWTIYMFLVWYEDFFGNGVKEAA

Sequences:

>Translated_614_residues
MCGFAGFTGYLADGDAVLERMMNKIIHRGPDSAGKYIDDKAYMGFRRLSIIDLDNGSQPMFNENKKIVITFNGEIYNYQD
LRKDLIEKGHVFANNSDTEVLIHSYEEYGKDMLNKLRGMFAFVIWDSEKETLFGARDFFGIKPFYYTVADGNMIYGSEIK
SILEHPAYKKEVNPVALENYLTFQYSVLDETFFKGIFKLMPGHFFTFHKGELNIERYWEPTFDADESKSLDEFVDEIDDV
MHDSVEHHKISDVEVGSFLSSGVDSSYVAATFNGDKTFTVGFDYEKYNEIDYAKALSDKIKIDNYSKLVSSEEYWAAIPK
IQYHMDEPLADPAAIALYFVSQTAAKHVKVAMSGEGADEFFGGYNIYREPLDLAEFQKLPKGLRKGLAGIANAIPFKFKG
KSFLNRASKTVEERFIGNAFMFNEKERARILKNPTGKYDHKQLTKPFYDKVADKDDVTKMQYIDINFWLIGDILLKADKM
SMAHSLEVRVPFLDKEVFNVARTIPTKYKVNKSNTKYAMRQAAHRHLPDMVAEKKKLGFPVPIRIWLKDEKYYNMIKKAF
TSEAAEKYFNTDEIVKYLDEHKEGKADNSRKIWTIYMFLVWYEDFFGNGVKEAA
>Mature_614_residues
MCGFAGFTGYLADGDAVLERMMNKIIHRGPDSAGKYIDDKAYMGFRRLSIIDLDNGSQPMFNENKKIVITFNGEIYNYQD
LRKDLIEKGHVFANNSDTEVLIHSYEEYGKDMLNKLRGMFAFVIWDSEKETLFGARDFFGIKPFYYTVADGNMIYGSEIK
SILEHPAYKKEVNPVALENYLTFQYSVLDETFFKGIFKLMPGHFFTFHKGELNIERYWEPTFDADESKSLDEFVDEIDDV
MHDSVEHHKISDVEVGSFLSSGVDSSYVAATFNGDKTFTVGFDYEKYNEIDYAKALSDKIKIDNYSKLVSSEEYWAAIPK
IQYHMDEPLADPAAIALYFVSQTAAKHVKVAMSGEGADEFFGGYNIYREPLDLAEFQKLPKGLRKGLAGIANAIPFKFKG
KSFLNRASKTVEERFIGNAFMFNEKERARILKNPTGKYDHKQLTKPFYDKVADKDDVTKMQYIDINFWLIGDILLKADKM
SMAHSLEVRVPFLDKEVFNVARTIPTKYKVNKSNTKYAMRQAAHRHLPDMVAEKKKLGFPVPIRIWLKDEKYYNMIKKAF
TSEAAEKYFNTDEIVKYLDEHKEGKADNSRKIWTIYMFLVWYEDFFGNGVKEAA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=394, Percent_Identity=26.9035532994924, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI168229252, Length=394, Percent_Identity=26.9035532994924, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI168229250, Length=394, Percent_Identity=26.9035532994924, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI296010848, Length=371, Percent_Identity=26.9541778975741, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI296010852, Length=298, Percent_Identity=25.8389261744966, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI296010850, Length=298, Percent_Identity=25.8389261744966, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1786889, Length=445, Percent_Identity=28.7640449438202, Blast_Score=156, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17560178, Length=400, Percent_Identity=27.25, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71993933, Length=368, Percent_Identity=29.0760869565217, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25147557, Length=368, Percent_Identity=29.0760869565217, Blast_Score=117, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI25147560, Length=271, Percent_Identity=29.1512915129151, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321563, Length=400, Percent_Identity=26.5, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6325403, Length=401, Percent_Identity=23.9401496259352, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI45553209, Length=443, Percent_Identity=27.313769751693, Blast_Score=138, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70828; Mature: 70828

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGFAGFTGYLADGDAVLERMMNKIIHRGPDSAGKYIDDKAYMGFRRLSIIDLDNGSQPM
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEEEEECCCCCCCC
FNENKKIVITFNGEIYNYQDLRKDLIEKGHVFANNSDTEVLIHSYEEYGKDMLNKLRGMF
CCCCCEEEEEECCCEECHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHHHHHHHHHHHEE
AFVIWDSEKETLFGARDFFGIKPFYYTVADGNMIYGSEIKSILEHPAYKKEVNPVALENY
EEEEECCCCCCEECCHHHCCCCCEEEEEECCCEEEHHHHHHHHHCCCHHHCCCCEEEHHE
LTFQYSVLDETFFKGIFKLMPGHFFTFHKGELNIERYWEPTFDADESKSLDEFVDEIDDV
EEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCCCCCCCCCHHHHHHHHHHH
MHDSVEHHKISDVEVGSFLSSGVDSSYVAATFNGDKTFTVGFDYEKYNEIDYAKALSDKI
HHCCHHHCCCCCCHHHHHHHCCCCCCEEEEEECCCEEEEEECCHHHHCCHHHHHHHHHHC
KIDNYSKLVSSEEYWAAIPKIQYHMDEPLADPAAIALYFVSQTAAKHVKVAMSGEGADEF
CCCCHHHHHCCCHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHH
FGGYNIYREPLDLAEFQKLPKGLRKGLAGIANAIPFKFKGKSFLNRASKTVEERFIGNAF
HCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCCCE
MFNEKERARILKNPTGKYDHKQLTKPFYDKVADKDDVTKMQYIDINFWLIGDILLKADKM
EECCHHHHHHHCCCCCCCCHHHHCCHHHHHHCCCCCCCEEEEEEEEEEEEHHHHHHHHHH
SMAHSLEVRVPFLDKEVFNVARTIPTKYKVNKSNTKYAMRQAAHRHLPDMVAEKKKLGFP
HHHHEEEEEECCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHCCCC
VPIRIWLKDEKYYNMIKKAFTSEAAEKYFNTDEIVKYLDEHKEGKADNSRKIWTIYMFLV
CEEEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHH
WYEDFFGNGVKEAA
HHHHHHCCCCCCCC
>Mature Secondary Structure
MCGFAGFTGYLADGDAVLERMMNKIIHRGPDSAGKYIDDKAYMGFRRLSIIDLDNGSQPM
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEEEEECCCCCCCC
FNENKKIVITFNGEIYNYQDLRKDLIEKGHVFANNSDTEVLIHSYEEYGKDMLNKLRGMF
CCCCCEEEEEECCCEECHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHHHHHHHHHHHEE
AFVIWDSEKETLFGARDFFGIKPFYYTVADGNMIYGSEIKSILEHPAYKKEVNPVALENY
EEEEECCCCCCEECCHHHCCCCCEEEEEECCCEEEHHHHHHHHHCCCHHHCCCCEEEHHE
LTFQYSVLDETFFKGIFKLMPGHFFTFHKGELNIERYWEPTFDADESKSLDEFVDEIDDV
EEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCCCCCCCCCHHHHHHHHHHH
MHDSVEHHKISDVEVGSFLSSGVDSSYVAATFNGDKTFTVGFDYEKYNEIDYAKALSDKI
HHCCHHHCCCCCCHHHHHHHCCCCCCEEEEEECCCEEEEEECCHHHHCCHHHHHHHHHHC
KIDNYSKLVSSEEYWAAIPKIQYHMDEPLADPAAIALYFVSQTAAKHVKVAMSGEGADEF
CCCCHHHHHCCCHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHH
FGGYNIYREPLDLAEFQKLPKGLRKGLAGIANAIPFKFKGKSFLNRASKTVEERFIGNAF
HCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCCCE
MFNEKERARILKNPTGKYDHKQLTKPFYDKVADKDDVTKMQYIDINFWLIGDILLKADKM
EECCHHHHHHHCCCCCCCCHHHHCCHHHHHHCCCCCCCEEEEEEEEEEEEHHHHHHHHHH
SMAHSLEVRVPFLDKEVFNVARTIPTKYKVNKSNTKYAMRQAAHRHLPDMVAEKKKLGFP
HHHHEEEEEECCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHCCCC
VPIRIWLKDEKYYNMIKKAFTSEAAEKYFNTDEIVKYLDEHKEGKADNSRKIWTIYMFLV
CEEEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHH
WYEDFFGNGVKEAA
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]