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The map label for this gene is eda [H]

Identifier: 238917181

GI number: 238917181

Start: 1283338

End: 1283997

Strand: Reverse

Name: eda [H]

Synonym: EUBELI_01254

Alternate gene names: 238917181

Gene position: 1283997-1283338 (Counterclockwise)

Preceding gene: 238917182

Following gene: 238917179

Centisome position: 59.88

GC content: 41.67

Gene sequence:

>660_bases
ATGAAAGGAGTCTATATAATTATGAGCAATATTGCAGACAAATTTGAAGAACTTGGAGTAATTCCAGTAGTAGTATTGAA
TGATGCTAAAGATGCACTTCCATTAGCTAAGGCTTTAGTTGATGGTGGTCTTCCTTGTGCAGAGGTTACATTCCGTACAG
CAGCAGCTGAGGAATCTATCCGTCTTATGCATGAAGCATATCCAGATATGGTACTTGCAGCAGGTACAGTACTTACAACA
GAGCAGGTTGACAGAGCTGTAGCAGCTGGTGCTTCAGTTATCGTAAGCCCAGGATTTGATCCAGAAATCGTTGATTACTG
TATTTCTAAGAACATTCCTGTTATGCCAGGTATCGTTACACCTTCAGAGCTTGCACAGGCTGTTAAGAGAGGCCTTACAA
GAGTTAAATTCTTCCCAGCTACAGCTGCAGGTGGAATTAAGATGATCAAGGCTATGTGCGCAGCTTACACTAATGTAAGA
ATCATGCCTACAGGTGGTATCAACACAGCTAACCTTGAGGAATTCTTATCATGCGATAAGATATTCTGCTGTGGTGGTTC
ATGGATGGTTAAGGGTGATATGATTAAGGCAGGAGAATTTGACAAGATTAAGGATATGACAGCAGAGGCTGTTGCTCTTG
TTAAGAAGATCCGTGGCTAA

Upstream 100 bases:

>100_bases
TGCCGTTGCTGAGGGAATAACAACATTTATAATGCTTGTAATGGTGCTGAGAGTGTGATAGAATATTTGCGGACAAAATT
ATCCCAGAGATTGGGAAAAT

Downstream 100 bases:

>100_bases
TTACCAGTGATTGTTAATAGCAAAGATACATATAAACCCCGTATTCTGTTTTGCAGGATACGGGGTTTTGTACGTATATT
ATGTTTTTTAGTGAATTACA

Product: 2-dehydro-3-deoxyphosphogluconate aldolase / 4-hydroxy-2-oxoglutarate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKGVYIIMSNIADKFEELGVIPVVVLNDAKDALPLAKALVDGGLPCAEVTFRTAAAEESIRLMHEAYPDMVLAAGTVLTT
EQVDRAVAAGASVIVSPGFDPEIVDYCISKNIPVMPGIVTPSELAQAVKRGLTRVKFFPATAAGGIKMIKAMCAAYTNVR
IMPTGGINTANLEEFLSCDKIFCCGGSWMVKGDMIKAGEFDKIKDMTAEAVALVKKIRG

Sequences:

>Translated_219_residues
MKGVYIIMSNIADKFEELGVIPVVVLNDAKDALPLAKALVDGGLPCAEVTFRTAAAEESIRLMHEAYPDMVLAAGTVLTT
EQVDRAVAAGASVIVSPGFDPEIVDYCISKNIPVMPGIVTPSELAQAVKRGLTRVKFFPATAAGGIKMIKAMCAAYTNVR
IMPTGGINTANLEEFLSCDKIFCCGGSWMVKGDMIKAGEFDKIKDMTAEAVALVKKIRG
>Mature_219_residues
MKGVYIIMSNIADKFEELGVIPVVVLNDAKDALPLAKALVDGGLPCAEVTFRTAAAEESIRLMHEAYPDMVLAAGTVLTT
EQVDRAVAAGASVIVSPGFDPEIVDYCISKNIPVMPGIVTPSELAQAVKRGLTRVKFFPATAAGGIKMIKAMCAAYTNVR
IMPTGGINTANLEEFLSCDKIFCCGGSWMVKGDMIKAGEFDKIKDMTAEAVALVKKIRG

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=182, Percent_Identity=41.2087912087912, Blast_Score=154, Evalue=3e-39,
Organism=Escherichia coli, GI48994954, Length=190, Percent_Identity=30, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 23247; Mature: 23247

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGVYIIMSNIADKFEELGVIPVVVLNDAKDALPLAKALVDGGLPCAEVTFRTAAAEESI
CCCEEEEHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RLMHEAYPDMVLAAGTVLTTEQVDRAVAAGASVIVSPGFDPEIVDYCISKNIPVMPGIVT
HHHHHHCCCHHHHCCCEEEHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCC
PSELAQAVKRGLTRVKFFPATAAGGIKMIKAMCAAYTNVRIMPTGGINTANLEEFLSCDK
HHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCCC
IFCCGGSWMVKGDMIKAGEFDKIKDMTAEAVALVKKIRG
EEEECCCEEEECCEEECCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGVYIIMSNIADKFEELGVIPVVVLNDAKDALPLAKALVDGGLPCAEVTFRTAAAEESI
CCCEEEEHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RLMHEAYPDMVLAAGTVLTTEQVDRAVAAGASVIVSPGFDPEIVDYCISKNIPVMPGIVT
HHHHHHCCCHHHHCCCEEEHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCC
PSELAQAVKRGLTRVKFFPATAAGGIKMIKAMCAAYTNVRIMPTGGINTANLEEFLSCDK
HHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCCC
IFCCGGSWMVKGDMIKAGEFDKIKDMTAEAVALVKKIRG
EEEECCCEEEECCEEECCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]