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The map label for this gene is pgk [H]

Identifier: 238917118

GI number: 238917118

Start: 1201244

End: 1202452

Strand: Reverse

Name: pgk [H]

Synonym: EUBELI_01189

Alternate gene names: 238917118

Gene position: 1202452-1201244 (Counterclockwise)

Preceding gene: 238917119

Following gene: 238917117

Centisome position: 56.08

GC content: 38.54

Gene sequence:

>1209_bases
ATGGAGGATATTAATATGCTTAATAAAAAGTCTATTGATGATATCAATGTAAAGGGCAAAAGAGTATTATGCAGATGTGA
TTTTAATGTACCTTTAGATGGAAGCAGAATTACAGATGAAACAAGACTTGTAGCTGCTCTTCCAACAATCAAGAAGCTTA
TTGCTGATGGAGGAAAGGTTATTCTTTGTTCACATCTTGGAAAGCCAAAGGGACCAGATGCTGCATTTTCTCTTGCACCA
GTTGCAAAGAGACTTTCAGAACTTCTTGGTCAGGAAGTTAAGTTTGCAGCAGATGATACTGTTGTAGGCGATAATGCAAG
AGCAGCTGTTGCTGCAATGAATGATGGAGATGTTATTCTTCTTGAAAATACAAGATTCAGACCAGAAGAGACTAAGAACG
GTGAAGCATTTTCTAAGGATTTAGCTTCTATTGCAGATGTATTTGTTAATGATGCATTTGGTACAGCTCACAGAGCGCAT
TGTTCTAATGTAGGTGTTACACAGTTTGTTGATACAGCTGTTGTTGGATATCTTATGCAGAAGGAAATTGATTTCCTTGG
AAATGCAGTTAATAATCCTGTAAGACCTTTCGTTGCAATTCTTGGTGGTTCAAAGGTTTCTTCTAAGATTTCAGTTATCA
ATAATCTTCTTGATAAGGTTGACACACTTATTATCGGTGGTGGTATGTCTTATACATTCCAGAAGGCACATGGTGGTAAT
GTAGGTAAGTCATTAGTAGAGGATGATTATCTTGATTTTGCTAAGGAAATGATGAAGAAGGCTGAAGAAAAGGGCGTTAA
AATGCTTATTCCTATCGATACAGTTGTAGCTAAGGAATTCAGCAATGATTCTCCTTCACATGTCGTAGAAGCAGGACATC
AGGCAGATGATGACATGGGTATGGATATTGGACCTAAGACATGTGAGCTTTACAAGGAAGCTTTAAAGGATGCCAAGACT
GTAGTATGGAATGGACCAATGGGAGTATTTGAATTCCCTAATTTCGCAGCAGGAACAATTGCTGTTGCAGAAGAACTTGC
ACAGCTTAAAGATGCTACAACAATCATTGGTGGTGGTGATTCAGCAGCTGCTGTTAACAACTTAGGATTTGGAGATAAGA
TGACACACATCTCTACAGGTGGTGGAGCATCTCTTGAATTCCTTGAAGGTAAAGAACTTCCAGGCGTAGTTGCAGCTAAC
GATAAGTAA

Upstream 100 bases:

>100_bases
GCTGAATTAAACTAATCATTTAGATTGTTTTTGCCGGAGAATGGTATGACTATTCTCCGGTTTTTTATAAAGCTGTATAT
GTAGCTATTTTATGTATTAT

Downstream 100 bases:

>100_bases
TTAATTTTACTGACATAGTTATATAATTATATTTTAATCCGGACCTTATTTTTGTTAAGTAGGGTCTGGATTTTTTGTTA
TTTATTTCACAAACTATACT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MEDINMLNKKSIDDINVKGKRVLCRCDFNVPLDGSRITDETRLVAALPTIKKLIADGGKVILCSHLGKPKGPDAAFSLAP
VAKRLSELLGQEVKFAADDTVVGDNARAAVAAMNDGDVILLENTRFRPEETKNGEAFSKDLASIADVFVNDAFGTAHRAH
CSNVGVTQFVDTAVVGYLMQKEIDFLGNAVNNPVRPFVAILGGSKVSSKISVINNLLDKVDTLIIGGGMSYTFQKAHGGN
VGKSLVEDDYLDFAKEMMKKAEEKGVKMLIPIDTVVAKEFSNDSPSHVVEAGHQADDDMGMDIGPKTCELYKEALKDAKT
VVWNGPMGVFEFPNFAAGTIAVAEELAQLKDATTIIGGGDSAAAVNNLGFGDKMTHISTGGGASLEFLEGKELPGVVAAN
DK

Sequences:

>Translated_402_residues
MEDINMLNKKSIDDINVKGKRVLCRCDFNVPLDGSRITDETRLVAALPTIKKLIADGGKVILCSHLGKPKGPDAAFSLAP
VAKRLSELLGQEVKFAADDTVVGDNARAAVAAMNDGDVILLENTRFRPEETKNGEAFSKDLASIADVFVNDAFGTAHRAH
CSNVGVTQFVDTAVVGYLMQKEIDFLGNAVNNPVRPFVAILGGSKVSSKISVINNLLDKVDTLIIGGGMSYTFQKAHGGN
VGKSLVEDDYLDFAKEMMKKAEEKGVKMLIPIDTVVAKEFSNDSPSHVVEAGHQADDDMGMDIGPKTCELYKEALKDAKT
VVWNGPMGVFEFPNFAAGTIAVAEELAQLKDATTIIGGGDSAAAVNNLGFGDKMTHISTGGGASLEFLEGKELPGVVAAN
DK
>Mature_402_residues
MEDINMLNKKSIDDINVKGKRVLCRCDFNVPLDGSRITDETRLVAALPTIKKLIADGGKVILCSHLGKPKGPDAAFSLAP
VAKRLSELLGQEVKFAADDTVVGDNARAAVAAMNDGDVILLENTRFRPEETKNGEAFSKDLASIADVFVNDAFGTAHRAH
CSNVGVTQFVDTAVVGYLMQKEIDFLGNAVNNPVRPFVAILGGSKVSSKISVINNLLDKVDTLIIGGGMSYTFQKAHGGN
VGKSLVEDDYLDFAKEMMKKAEEKGVKMLIPIDTVVAKEFSNDSPSHVVEAGHQADDDMGMDIGPKTCELYKEALKDAKT
VVWNGPMGVFEFPNFAAGTIAVAEELAQLKDATTIIGGGDSAAAVNNLGFGDKMTHISTGGGASLEFLEGKELPGVVAAN
DK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=417, Percent_Identity=46.2829736211031, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI31543397, Length=421, Percent_Identity=44.4180522565321, Blast_Score=328, Evalue=6e-90,
Organism=Escherichia coli, GI1789294, Length=391, Percent_Identity=43.4782608695652, Blast_Score=308, Evalue=5e-85,
Organism=Caenorhabditis elegans, GI17508823, Length=414, Percent_Identity=46.1352657004831, Blast_Score=350, Evalue=7e-97,
Organism=Saccharomyces cerevisiae, GI6319857, Length=424, Percent_Identity=46.2264150943396, Blast_Score=354, Evalue=2e-98,
Organism=Drosophila melanogaster, GI17136394, Length=414, Percent_Identity=44.6859903381643, Blast_Score=314, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24581203, Length=417, Percent_Identity=42.9256594724221, Blast_Score=309, Evalue=2e-84,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42691; Mature: 42691

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDINMLNKKSIDDINVKGKRVLCRCDFNVPLDGSRITDETRLVAALPTIKKLIADGGKV
CCCCHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCE
ILCSHLGKPKGPDAAFSLAPVAKRLSELLGQEVKFAADDTVVGDNARAAVAAMNDGDVIL
EEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCEECCCCCEEEEEECCCCEEE
LENTRFRPEETKNGEAFSKDLASIADVFVNDAFGTAHRAHCSNVGVTQFVDTAVVGYLMQ
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHH
KEIDFLGNAVNNPVRPFVAILGGSKVSSKISVINNLLDKVDTLIIGGGMSYTFQKAHGGN
HHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCC
VGKSLVEDDYLDFAKEMMKKAEEKGVKMLIPIDTVVAKEFSNDSPSHVVEAGHQADDDMG
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCHHHHHCCCCCCCCCC
MDIGPKTCELYKEALKDAKTVVWNGPMGVFEFPNFAAGTIAVAEELAQLKDATTIIGGGD
CCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCC
SAAAVNNLGFGDKMTHISTGGGASLEFLEGKELPGVVAANDK
CHHHHHCCCCCCCCCEEECCCCCEEEEECCCCCCCEEECCCC
>Mature Secondary Structure
MEDINMLNKKSIDDINVKGKRVLCRCDFNVPLDGSRITDETRLVAALPTIKKLIADGGKV
CCCCHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCE
ILCSHLGKPKGPDAAFSLAPVAKRLSELLGQEVKFAADDTVVGDNARAAVAAMNDGDVIL
EEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCEECCCCCEEEEEECCCCEEE
LENTRFRPEETKNGEAFSKDLASIADVFVNDAFGTAHRAHCSNVGVTQFVDTAVVGYLMQ
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHH
KEIDFLGNAVNNPVRPFVAILGGSKVSSKISVINNLLDKVDTLIIGGGMSYTFQKAHGGN
HHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCC
VGKSLVEDDYLDFAKEMMKKAEEKGVKMLIPIDTVVAKEFSNDSPSHVVEAGHQADDDMG
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCHHHHHCCCCCCCCCC
MDIGPKTCELYKEALKDAKTVVWNGPMGVFEFPNFAAGTIAVAEELAQLKDATTIIGGGD
CCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCC
SAAAVNNLGFGDKMTHISTGGGASLEFLEGKELPGVVAANDK
CHHHHHCCCCCCCCCEEECCCCCEEEEECCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10463150; 11466286 [H]