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The map label for this gene is dacF [H]

Identifier: 238917030

GI number: 238917030

Start: 1110799

End: 1112046

Strand: Direct

Name: dacF [H]

Synonym: EUBELI_01100

Alternate gene names: 238917030

Gene position: 1110799-1112046 (Clockwise)

Preceding gene: 238916997

Following gene: 238917031

Centisome position: 51.81

GC content: 35.26

Gene sequence:

>1248_bases
ATGAAATCATTTAAGAATTTTTCAATTATTATACTCGTATTATCGTTGTTGGTTTGTTGCATATTCTGTCCTGTAACAAC
TCAGGCAGCAGGACTATATTCAAAATACTCTGTTCTTATTGATTCTGATTCAGGCAGGATACTTTCTGGCAGCAATGAAA
CAACGGCTGTAAGCATGGCAAGCACAACTAAAATTATGACTCTTATTATTGCTTTGGAAAACTGTGACAAGAATTTTGTC
GTAACAACTTCCGCCTACGCAGCTTCCATGCCTGATGTACAGCTTAACGCCGTAACTGGTGAGCAATTCATAATTAACGA
CCTATACTACTCACTTATGCTTGAATCACATAATGATTCCGCTGTAATAATTGCTGAAAATGCTGCTTATTACTTACTAT
GTAAAAATCCTTCTTTAAGGTCTGAAACTCCGTTTATCAATAATTATAATTACGATTCCTCATTTCTTTCTGAGATTAAC
CATGAACAAAGTGAAATACTTGTACATATATTTACCGGCCTTATGAATGAAAAAGCGGATGATATTCTCTTATCAGACAC
ATATTACATAACACCTAATGGTCTTGATGCCGAGGATAACTATAGTTTTCATCATACAACAGCATATGACCTTGCAAAAA
CCATGGCATATTGTATTAATAATGAGGATTTTTTATATATAACACAGACTGTTTCTAAAACAATTTCAGACACAACAGGC
AGATACCATTACCAGCTAAATAACAAAAACAGGCTTTTAAATCCTAATGAAGGAATTATCTCTGGAAAAACAGGTTTCAC
TAACAAAGCCGGATACTGCTATGTATGTGCATACAAAGACAAAAACCGGACACTGTGCATCGCCCTGCTTGCATGTGGCT
GGCCACCAAATAAGAATTACAAATGGAGTGACGCCAACTATCTTATTGCACTTGGTAAGAAATACAACAAGCAGAAATAT
TTTAATTGCGAGTACACATTTTACATTCCTGTATCTAAAGGAAAATATAGCTTCTGTGAACTTATTCCTGACAGTGCTGT
TGAGTTTCTTGCCTGCGATGACGATTCCGTAAATATCCAGATAGATATTCCAGAAACACTTACTGCTCCTGTCAATTCAG
CACAGATATATGGAACAATTAATATATATGTAAATAATGAGCCTGCCCGCAGTATTAATCTGAAAGCAAAGCAGAGTATT
CCCAAAAAATCCCATACACTGGATATATTTGATACAATATTTCGATAG

Upstream 100 bases:

>100_bases
TATTAAATCACATATTAATCTATATATAATAATACCTTATAGCACTCATAAATTCAAATCATTTTAATATAATCATAGAA
AGACATTTTATTAAATTTCT

Downstream 100 bases:

>100_bases
CAGCGCAAATTTGGGGATTATTTTACGAAAACACATGACACGGTTATAATTGTGTTATATAATTAAAAAGTCGGTTTTAA
CTGGCTAATAATATATGGAG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKSFKNFSIIILVLSLLVCCIFCPVTTQAAGLYSKYSVLIDSDSGRILSGSNETTAVSMASTTKIMTLIIALENCDKNFV
VTTSAYAASMPDVQLNAVTGEQFIINDLYYSLMLESHNDSAVIIAENAAYYLLCKNPSLRSETPFINNYNYDSSFLSEIN
HEQSEILVHIFTGLMNEKADDILLSDTYYITPNGLDAEDNYSFHHTTAYDLAKTMAYCINNEDFLYITQTVSKTISDTTG
RYHYQLNNKNRLLNPNEGIISGKTGFTNKAGYCYVCAYKDKNRTLCIALLACGWPPNKNYKWSDANYLIALGKKYNKQKY
FNCEYTFYIPVSKGKYSFCELIPDSAVEFLACDDDSVNIQIDIPETLTAPVNSAQIYGTINIYVNNEPARSINLKAKQSI
PKKSHTLDIFDTIFR

Sequences:

>Translated_415_residues
MKSFKNFSIIILVLSLLVCCIFCPVTTQAAGLYSKYSVLIDSDSGRILSGSNETTAVSMASTTKIMTLIIALENCDKNFV
VTTSAYAASMPDVQLNAVTGEQFIINDLYYSLMLESHNDSAVIIAENAAYYLLCKNPSLRSETPFINNYNYDSSFLSEIN
HEQSEILVHIFTGLMNEKADDILLSDTYYITPNGLDAEDNYSFHHTTAYDLAKTMAYCINNEDFLYITQTVSKTISDTTG
RYHYQLNNKNRLLNPNEGIISGKTGFTNKAGYCYVCAYKDKNRTLCIALLACGWPPNKNYKWSDANYLIALGKKYNKQKY
FNCEYTFYIPVSKGKYSFCELIPDSAVEFLACDDDSVNIQIDIPETLTAPVNSAQIYGTINIYVNNEPARSINLKAKQSI
PKKSHTLDIFDTIFR
>Mature_415_residues
MKSFKNFSIIILVLSLLVCCIFCPVTTQAAGLYSKYSVLIDSDSGRILSGSNETTAVSMASTTKIMTLIIALENCDKNFV
VTTSAYAASMPDVQLNAVTGEQFIINDLYYSLMLESHNDSAVIIAENAAYYLLCKNPSLRSETPFINNYNYDSSFLSEIN
HEQSEILVHIFTGLMNEKADDILLSDTYYITPNGLDAEDNYSFHHTTAYDLAKTMAYCINNEDFLYITQTVSKTISDTTG
RYHYQLNNKNRLLNPNEGIISGKTGFTNKAGYCYVCAYKDKNRTLCIALLACGWPPNKNYKWSDANYLIALGKKYNKQKY
FNCEYTFYIPVSKGKYSFCELIPDSAVEFLACDDDSVNIQIDIPETLTAPVNSAQIYGTINIYVNNEPARSINLKAKQSI
PKKSHTLDIFDTIFR

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 46633; Mature: 46633

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFKNFSIIILVLSLLVCCIFCPVTTQAAGLYSKYSVLIDSDSGRILSGSNETTAVSMA
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEEEEECCCCCEEECCCCCEEEEEH
STTKIMTLIIALENCDKNFVVTTSAYAASMPDVQLNAVTGEQFIINDLYYSLMLESHNDS
HHHEEEEEEEEEECCCCCEEEEECHHHCCCCCEEEEEECCCEEEHHHEEEEEEEECCCCC
AVIIAENAAYYLLCKNPSLRSETPFINNYNYDSSFLSEINHEQSEILVHIFTGLMNEKAD
EEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCC
DILLSDTYYITPNGLDAEDNYSFHHTTAYDLAKTMAYCINNEDFLYITQTVSKTISDTTG
CEEEECEEEECCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCC
RYHYQLNNKNRLLNPNEGIISGKTGFTNKAGYCYVCAYKDKNRTLCIALLACGWPPNKNY
EEEEEECCCCCCCCCCCCEEECCCCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCE
KWSDANYLIALGKKYNKQKYFNCEYTFYIPVSKGKYSFCELIPDSAVEFLACDDDSVNIQ
EECCCCEEEEECCCCCCCEEEEEEEEEEEEECCCCCHHEECCCCCCEEEEEECCCCEEEE
IDIPETLTAPVNSAQIYGTINIYVNNEPARSINLKAKQSIPKKSHTLDIFDTIFR
EECCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEHHHCCCCCCCEEHHHHHHCC
>Mature Secondary Structure
MKSFKNFSIIILVLSLLVCCIFCPVTTQAAGLYSKYSVLIDSDSGRILSGSNETTAVSMA
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEEEEECCCCCEEECCCCCEEEEEH
STTKIMTLIIALENCDKNFVVTTSAYAASMPDVQLNAVTGEQFIINDLYYSLMLESHNDS
HHHEEEEEEEEEECCCCCEEEEECHHHCCCCCEEEEEECCCEEEHHHEEEEEEEECCCCC
AVIIAENAAYYLLCKNPSLRSETPFINNYNYDSSFLSEINHEQSEILVHIFTGLMNEKAD
EEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCC
DILLSDTYYITPNGLDAEDNYSFHHTTAYDLAKTMAYCINNEDFLYITQTVSKTISDTTG
CEEEECEEEECCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCC
RYHYQLNNKNRLLNPNEGIISGKTGFTNKAGYCYVCAYKDKNRTLCIALLACGWPPNKNY
EEEEEECCCCCCCCCCCCEEECCCCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCE
KWSDANYLIALGKKYNKQKYFNCEYTFYIPVSKGKYSFCELIPDSAVEFLACDDDSVNIQ
EECCCCEEEEECCCCCCCEEEEEEEEEEEEECCCCCHHEECCCCCCEEEEEECCCCEEEE
IDIPETLTAPVNSAQIYGTINIYVNNEPARSINLKAKQSIPKKSHTLDIFDTIFR
EECCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEHHHCCCCCCCEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]