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The map label for this gene is recN [H]

Identifier: 238917010

GI number: 238917010

Start: 1093601

End: 1095274

Strand: Reverse

Name: recN [H]

Synonym: EUBELI_01080

Alternate gene names: 238917010

Gene position: 1095274-1093601 (Counterclockwise)

Preceding gene: 238917011

Following gene: 238917009

Centisome position: 51.08

GC content: 34.71

Gene sequence:

>1674_bases
ATGTTAGTTAATTTACATGTTAAGAATCTTGCACTTATAGAAGAAGAGAATATTGATTTTGATGAAGGACTTAATATACT
TTCTGGTGAAACCGGTGCAGGTAAATCTATTATTCTCGGTTCTATTAATGCTGCATTAGGAAGCAAGACATCACCTGATT
TTATAAGGTCAGGATGTGAATATGGACTTTCAGAGATAACATTTGCAATACCAGAAGATAAGATTGAACGAATCAAAGAA
CTGGGGGTTATATCATGCGATGACGGTGAACTTGTTATTTCGAGGAAAATTATGGCAAATCGTTCGCAGATTAAGGTTAA
CGGACAGTCTTTTACATCAGCCCAGACAAGAGTTCTTGCACATGAACTGATTGATATTCACGGACAACATGATAACCAGC
TGCTTATGGATGAGAGTAATCATTTGTCTGTAATTGATGATTATGATAACAGTATTAATCCACTTCTTGATTCGTATAAA
GAATGCTATAAGGAATATACTATATGCAAAAAAGCATATGAAAGTCTTTCTGGTGATGATGAATCAAGGATTAAAGAGAT
GGCTTTTCTTCAATATGAAATCAATGAGCTTGAATCAGCTGCTTTAAAACCTGGAGAAGATGAACAGATTGAAGCTGATT
ACCGGAGAATGAGTAACTTCCAGAAGATTGCAGGAAATCTTTCTGAGGTGTCGATGCTGCTTTCAGAAGGTGACAATAAT
CTTAGTGATATGGCAGGAGCTGCATTAAAGAATATGATAGCTGCAAGCGAATATGATGAATCTTTGAAAGATGACTGTGA
TATTCTAGCTGATATTGAGAATCTTATATCAGATGTGGCTCATAACCTTTCATCTTATATGGATGATTTTACATTTGACC
AGTCTGAATTCAATACACTTGAAGAAAGACTTGATATGATTAATTCTCTTAAGATGAAATATGGCAAGTCGATTGAAGAT
ATATTATCTGTTCTTGATAATAAGAAACAGAAGTTATCTGAATATGAGAATTATGATGAAGTTGTTGCTGCAAGAAAAGC
TGAATATGATAGAACTTATAAAAAACTTATGAATGCAGCTTTTCTTTTAAGCAGCCAGAGAAAGAAAACAGCACTAAATT
TTTCGCAGGATATAATAAATGCCTTAAAACAGCTTAATTTTCTTGATGTAAGATTTGAAGCTGAATTTGAAGAGAAAAAT
CCTGATTCAACCGGAACTGACAATGTCAGGTTTATGATATCAACTAATCCGGGTGAAGATATGAAGCCTCTTGCAAAGAT
TGCATCAGGTGGAGAATTGTCAAGAATCATGCTTGCTATTAAAAGTGTAATGGCAGAGACAGATGTATCCAAAACACTGA
TATTTGATGAGATTGATGCAGGAATCAGCGGAAAGACGGCACAGCTTGTTGCAGAGAAGTTAAATACACTCTCAAAAACT
CATCAGATAATATGCATTACCCATCTGCCTCAGATTGCGGCAATGGCTGATAATCACTATGTTATTGAGAAGTCTACTAA
AGATAATCGGACAATAAGTAACATAACAAAGCTTTCATATGATGAAAGTATAGGTGAGTTAGCAAGAATGCTTGGCGGTT
CAAGTATTACAGATGCAGTTGTGTCTAATGCAAAAGAACTTAAGAATATGGCACAGACGGCAAAAAATAATTAA

Upstream 100 bases:

>100_bases
TCATGTGTGCAGTAAGGACAGTTAATGATACTTTGCTTGTTATGAACAAGATTAAGAAACTTATTAAGAACAATTAATAG
ACAGAATTGAAAGGCAGGAT

Downstream 100 bases:

>100_bases
ATACATTTTAACTGAATTTACAGTAATACCCTTTTTTAAAAGTATTTCATTGGTCAATACTAATCTTAGAAGGATGGTGG
ATTGATATGAAGAATTTTAA

Product: DNA repair protein RecN

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MLVNLHVKNLALIEEENIDFDEGLNILSGETGAGKSIILGSINAALGSKTSPDFIRSGCEYGLSEITFAIPEDKIERIKE
LGVISCDDGELVISRKIMANRSQIKVNGQSFTSAQTRVLAHELIDIHGQHDNQLLMDESNHLSVIDDYDNSINPLLDSYK
ECYKEYTICKKAYESLSGDDESRIKEMAFLQYEINELESAALKPGEDEQIEADYRRMSNFQKIAGNLSEVSMLLSEGDNN
LSDMAGAALKNMIAASEYDESLKDDCDILADIENLISDVAHNLSSYMDDFTFDQSEFNTLEERLDMINSLKMKYGKSIED
ILSVLDNKKQKLSEYENYDEVVAARKAEYDRTYKKLMNAAFLLSSQRKKTALNFSQDIINALKQLNFLDVRFEAEFEEKN
PDSTGTDNVRFMISTNPGEDMKPLAKIASGGELSRIMLAIKSVMAETDVSKTLIFDEIDAGISGKTAQLVAEKLNTLSKT
HQIICITHLPQIAAMADNHYVIEKSTKDNRTISNITKLSYDESIGELARMLGGSSITDAVVSNAKELKNMAQTAKNN

Sequences:

>Translated_557_residues
MLVNLHVKNLALIEEENIDFDEGLNILSGETGAGKSIILGSINAALGSKTSPDFIRSGCEYGLSEITFAIPEDKIERIKE
LGVISCDDGELVISRKIMANRSQIKVNGQSFTSAQTRVLAHELIDIHGQHDNQLLMDESNHLSVIDDYDNSINPLLDSYK
ECYKEYTICKKAYESLSGDDESRIKEMAFLQYEINELESAALKPGEDEQIEADYRRMSNFQKIAGNLSEVSMLLSEGDNN
LSDMAGAALKNMIAASEYDESLKDDCDILADIENLISDVAHNLSSYMDDFTFDQSEFNTLEERLDMINSLKMKYGKSIED
ILSVLDNKKQKLSEYENYDEVVAARKAEYDRTYKKLMNAAFLLSSQRKKTALNFSQDIINALKQLNFLDVRFEAEFEEKN
PDSTGTDNVRFMISTNPGEDMKPLAKIASGGELSRIMLAIKSVMAETDVSKTLIFDEIDAGISGKTAQLVAEKLNTLSKT
HQIICITHLPQIAAMADNHYVIEKSTKDNRTISNITKLSYDESIGELARMLGGSSITDAVVSNAKELKNMAQTAKNN
>Mature_557_residues
MLVNLHVKNLALIEEENIDFDEGLNILSGETGAGKSIILGSINAALGSKTSPDFIRSGCEYGLSEITFAIPEDKIERIKE
LGVISCDDGELVISRKIMANRSQIKVNGQSFTSAQTRVLAHELIDIHGQHDNQLLMDESNHLSVIDDYDNSINPLLDSYK
ECYKEYTICKKAYESLSGDDESRIKEMAFLQYEINELESAALKPGEDEQIEADYRRMSNFQKIAGNLSEVSMLLSEGDNN
LSDMAGAALKNMIAASEYDESLKDDCDILADIENLISDVAHNLSSYMDDFTFDQSEFNTLEERLDMINSLKMKYGKSIED
ILSVLDNKKQKLSEYENYDEVVAARKAEYDRTYKKLMNAAFLLSSQRKKTALNFSQDIINALKQLNFLDVRFEAEFEEKN
PDSTGTDNVRFMISTNPGEDMKPLAKIASGGELSRIMLAIKSVMAETDVSKTLIFDEIDAGISGKTAQLVAEKLNTLSKT
HQIICITHLPQIAAMADNHYVIEKSTKDNRTISNITKLSYDESIGELARMLGGSSITDAVVSNAKELKNMAQTAKNN

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=557, Percent_Identity=31.4183123877917, Blast_Score=277, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 62032; Mature: 62032

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVNLHVKNLALIEEENIDFDEGLNILSGETGAGKSIILGSINAALGSKTSPDFIRSGCE
CEEEEEECCEEEEECCCCCHHHCCCEECCCCCCCCEEEEECHHHHHCCCCCHHHHHHHHH
YGLSEITFAIPEDKIERIKELGVISCDDGELVISRKIMANRSQIKVNGQSFTSAQTRVLA
HCHHHEEEECCHHHHHHHHHCCCEECCCCCEEEEHHHHCCCCEEEECCCHHHHHHHHHHH
HELIDIHGQHDNQLLMDESNHLSVIDDYDNSINPLLDSYKECYKEYTICKKAYESLSGDD
HHHHHHCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ESRIKEMAFLQYEINELESAALKPGEDEQIEADYRRMSNFQKIAGNLSEVSMLLSEGDNN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
LSDMAGAALKNMIAASEYDESLKDDCDILADIENLISDVAHNLSSYMDDFTFDQSEFNTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EERLDMINSLKMKYGKSIEDILSVLDNKKQKLSEYENYDEVVAARKAEYDRTYKKLMNAA
HHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
FLLSSQRKKTALNFSQDIINALKQLNFLDVRFEAEFEEKNPDSTGTDNVRFMISTNPGED
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCC
MKPLAKIASGGELSRIMLAIKSVMAETDVSKTLIFDEIDAGISGKTAQLVAEKLNTLSKT
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
HQIICITHLPQIAAMADNHYVIEKSTKDNRTISNITKLSYDESIGELARMLGGSSITDAV
CCEEEEECCCHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VSNAKELKNMAQTAKNN
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLVNLHVKNLALIEEENIDFDEGLNILSGETGAGKSIILGSINAALGSKTSPDFIRSGCE
CEEEEEECCEEEEECCCCCHHHCCCEECCCCCCCCEEEEECHHHHHCCCCCHHHHHHHHH
YGLSEITFAIPEDKIERIKELGVISCDDGELVISRKIMANRSQIKVNGQSFTSAQTRVLA
HCHHHEEEECCHHHHHHHHHCCCEECCCCCEEEEHHHHCCCCEEEECCCHHHHHHHHHHH
HELIDIHGQHDNQLLMDESNHLSVIDDYDNSINPLLDSYKECYKEYTICKKAYESLSGDD
HHHHHHCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ESRIKEMAFLQYEINELESAALKPGEDEQIEADYRRMSNFQKIAGNLSEVSMLLSEGDNN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
LSDMAGAALKNMIAASEYDESLKDDCDILADIENLISDVAHNLSSYMDDFTFDQSEFNTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EERLDMINSLKMKYGKSIEDILSVLDNKKQKLSEYENYDEVVAARKAEYDRTYKKLMNAA
HHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
FLLSSQRKKTALNFSQDIINALKQLNFLDVRFEAEFEEKNPDSTGTDNVRFMISTNPGED
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCC
MKPLAKIASGGELSRIMLAIKSVMAETDVSKTLIFDEIDAGISGKTAQLVAEKLNTLSKT
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
HQIICITHLPQIAAMADNHYVIEKSTKDNRTISNITKLSYDESIGELARMLGGSSITDAV
CCEEEEECCCHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VSNAKELKNMAQTAKNN
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]