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The map label for this gene is spoIVB [H]

Identifier: 238917009

GI number: 238917009

Start: 1092264

End: 1093514

Strand: Reverse

Name: spoIVB [H]

Synonym: EUBELI_01079

Alternate gene names: 238917009

Gene position: 1093514-1092264 (Counterclockwise)

Preceding gene: 238917010

Following gene: 238917008

Centisome position: 51.0

GC content: 36.53

Gene sequence:

>1251_bases
ATGAAGAATTTTAAAAAAGGGTTAATTTATGCCGGGGTTCTGGCATTTTGCATAGGTTTTGGAGCTTATATATACTGTAA
TTCAATTAAGAAAAAGATTCCAGATAAGCTATATATATATAAGCAGGATAAGGCTGTTTTTTCTATGGACATACCTGTTG
TTGGTACTGTATATGACAGCAGTAATAATATATGTGCCGATAATATTGATTTTGGAAAGCAGGTTACAATTCAGTCGGGA
AATACCGGTCAGTATTATGTGGATTATAAGCTTTTTGGACTGCTTTCTGTCGCAAGGACACATATGGAGGTAGTCGATGA
AAAATACATTTATTCCGGAGGTTTTCAGGTCGGTATATATCTTAAATGTAATGGAGTATATGTTGTTAATACAGAAACAA
TATGCACATATGACGGGCAAAATGTTGTTCCTGCCAAAGGAAAGATTAATAAAGGTGATTATATAATTAAAGTTAATGGC
TCTCAGACAGATACGAAAGAACAGCTTTTACAGGCGGTGTCAGAATCGGCGGGAAATAGTATGGACATAACCGTCAGGCG
GGATGGTCAGGAAATTGAAGAGCAGATTATACCTGTGAAAAATGTTGCAGGGGAATACAAACTTGGAATCTGGGTTAAAG
ATGATACACAGGGTGTTGGAACTGTTACTTATGTGTGTGAAGATGGCACATTTGCAGCATTAGGACATGGAATATCGGAT
AACGAAACAGGGAATGTGCTTGATATAAGAGATGGCATGATATACAGAACAAGAATTCTGTCTATTGTACCCGGAAAAAA
TGGAGAGCCTGGAGAGCTTCTTGGTACGATTGATTATAGAGAGGACAACATAGGAAGTATAAGCCGTAATACTGATAAAG
GCATATATGGTGAAAATGCGTACTCTTTATATAAGGAGTATTCACCAGAACTGATGAAAGCAGCCGGGTCATATGAAGCT
TCAAAAGGAACAGCATATGTGAGGTTTTATAACAATGGCAGTTTTCATGATTATGAAATTGATATAACAGATTTAAAGTA
TGGCGATTCTAAGAATATAACATTTAAGGTGACATCAGGAGAGCTTCTCAAGCTGACTAATGGAATTGTACAGGGGATGA
GTGGGAGCCCGATTATTCAGAATGGAAAGATAATTGGGGCTGTCACACATGTATTTGTAGATGATTCTACTTGCGGATAT
GGTATATTTATTGATAGAATGCTTGATAAAGACGAAAAAGAAACTAATTAA

Upstream 100 bases:

>100_bases
CAAAAAATAATTAAATACATTTTAACTGAATTTACAGTAATACCCTTTTTTAAAAGTATTTCATTGGTCAATACTAATCT
TAGAAGGATGGTGGATTGAT

Downstream 100 bases:

>100_bases
GAAAAGGATTATGATACGATGGAAAGAGAGAAATTTGGCAGCAGACTTGGATTCATACTTATAAGTGCAGGCTGCGCAAT
CGGAATTGGAAATGTATGGC

Product: stage IV sporulation protein B

Products: NA

Alternate protein names: Sporulation factor IV B protease; Stage IV sporulation protein B [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKNFKKGLIYAGVLAFCIGFGAYIYCNSIKKKIPDKLYIYKQDKAVFSMDIPVVGTVYDSSNNICADNIDFGKQVTIQSG
NTGQYYVDYKLFGLLSVARTHMEVVDEKYIYSGGFQVGIYLKCNGVYVVNTETICTYDGQNVVPAKGKINKGDYIIKVNG
SQTDTKEQLLQAVSESAGNSMDITVRRDGQEIEEQIIPVKNVAGEYKLGIWVKDDTQGVGTVTYVCEDGTFAALGHGISD
NETGNVLDIRDGMIYRTRILSIVPGKNGEPGELLGTIDYREDNIGSISRNTDKGIYGENAYSLYKEYSPELMKAAGSYEA
SKGTAYVRFYNNGSFHDYEIDITDLKYGDSKNITFKVTSGELLKLTNGIVQGMSGSPIIQNGKIIGAVTHVFVDDSTCGY
GIFIDRMLDKDEKETN

Sequences:

>Translated_416_residues
MKNFKKGLIYAGVLAFCIGFGAYIYCNSIKKKIPDKLYIYKQDKAVFSMDIPVVGTVYDSSNNICADNIDFGKQVTIQSG
NTGQYYVDYKLFGLLSVARTHMEVVDEKYIYSGGFQVGIYLKCNGVYVVNTETICTYDGQNVVPAKGKINKGDYIIKVNG
SQTDTKEQLLQAVSESAGNSMDITVRRDGQEIEEQIIPVKNVAGEYKLGIWVKDDTQGVGTVTYVCEDGTFAALGHGISD
NETGNVLDIRDGMIYRTRILSIVPGKNGEPGELLGTIDYREDNIGSISRNTDKGIYGENAYSLYKEYSPELMKAAGSYEA
SKGTAYVRFYNNGSFHDYEIDITDLKYGDSKNITFKVTSGELLKLTNGIVQGMSGSPIIQNGKIIGAVTHVFVDDSTCGY
GIFIDRMLDKDEKETN
>Mature_416_residues
MKNFKKGLIYAGVLAFCIGFGAYIYCNSIKKKIPDKLYIYKQDKAVFSMDIPVVGTVYDSSNNICADNIDFGKQVTIQSG
NTGQYYVDYKLFGLLSVARTHMEVVDEKYIYSGGFQVGIYLKCNGVYVVNTETICTYDGQNVVPAKGKINKGDYIIKVNG
SQTDTKEQLLQAVSESAGNSMDITVRRDGQEIEEQIIPVKNVAGEYKLGIWVKDDTQGVGTVTYVCEDGTFAALGHGISD
NETGNVLDIRDGMIYRTRILSIVPGKNGEPGELLGTIDYREDNIGSISRNTDKGIYGENAYSLYKEYSPELMKAAGSYEA
SKGTAYVRFYNNGSFHDYEIDITDLKYGDSKNITFKVTSGELLKLTNGIVQGMSGSPIIQNGKIIGAVTHVFVDDSTCGY
GIFIDRMLDKDEKETN

Specific function: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by c

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Forespore intermembrane space [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase S55 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR014219
- InterPro:   IPR008763 [H]

Pfam domain/function: PF00595 PDZ; PF05580 Peptidase_S55 [H]

EC number: =3.4.21.116 [H]

Molecular weight: Translated: 45847; Mature: 45847

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNFKKGLIYAGVLAFCIGFGAYIYCNSIKKKIPDKLYIYKQDKAVFSMDIPVVGTVYDS
CCCHHHHHHHHHHHHHHHHCCCEEEECCHHHHCCCEEEEEEECCEEEEECCCEEEEEEEC
SNNICADNIDFGKQVTIQSGNTGQYYVDYKLFGLLSVARTHMEVVDEKYIYSGGFQVGIY
CCCEEECCCCCCCEEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHEEEECCEEEEEE
LKCNGVYVVNTETICTYDGQNVVPAKGKINKGDYIIKVNGSQTDTKEQLLQAVSESAGNS
EEECCEEEEECCEEEEECCCEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCE
MDITVRRDGQEIEEQIIPVKNVAGEYKLGIWVKDDTQGVGTVTYVCEDGTFAALGHGISD
EEEEEECCCHHHHHHCCCHHHCCCCEEEEEEEECCCCCCEEEEEEECCCCEEEECCCCCC
NETGNVLDIRDGMIYRTRILSIVPGKNGEPGELLGTIDYREDNIGSISRNTDKGIYGENA
CCCCCEEEECCCEEEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCH
YSLYKEYSPELMKAAGSYEASKGTAYVRFYNNGSFHDYEIDITDLKYGDSKNITFKVTSG
HHHHHHCCHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEEEEEEECCCCCEEEEECCC
ELLKLTNGIVQGMSGSPIIQNGKIIGAVTHVFVDDSTCGYGIFIDRMLDKDEKETN
CEEEEHHHHHCCCCCCCEEECCEEEEEEEEEEEECCCCCCEEEEEHHHCCCCCCCC
>Mature Secondary Structure
MKNFKKGLIYAGVLAFCIGFGAYIYCNSIKKKIPDKLYIYKQDKAVFSMDIPVVGTVYDS
CCCHHHHHHHHHHHHHHHHCCCEEEECCHHHHCCCEEEEEEECCEEEEECCCEEEEEEEC
SNNICADNIDFGKQVTIQSGNTGQYYVDYKLFGLLSVARTHMEVVDEKYIYSGGFQVGIY
CCCEEECCCCCCCEEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHEEEECCEEEEEE
LKCNGVYVVNTETICTYDGQNVVPAKGKINKGDYIIKVNGSQTDTKEQLLQAVSESAGNS
EEECCEEEEECCEEEEECCCEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCE
MDITVRRDGQEIEEQIIPVKNVAGEYKLGIWVKDDTQGVGTVTYVCEDGTFAALGHGISD
EEEEEECCCHHHHHHCCCHHHCCCCEEEEEEEECCCCCCEEEEEEECCCCEEEECCCCCC
NETGNVLDIRDGMIYRTRILSIVPGKNGEPGELLGTIDYREDNIGSISRNTDKGIYGENA
CCCCCEEEECCCEEEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCH
YSLYKEYSPELMKAAGSYEASKGTAYVRFYNNGSFHDYEIDITDLKYGDSKNITFKVTSG
HHHHHHCCHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEEEEEEECCCCCEEEEECCC
ELLKLTNGIVQGMSGSPIIQNGKIIGAVTHVFVDDSTCGYGIFIDRMLDKDEKETN
CEEEEHHHHHCCCCCCCEEECCEEEEEEEEEEEECCCCCCEEEEEHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 11741860 [H]