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The map label for this gene is pycB [H]

Identifier: 238916855

GI number: 238916855

Start: 939186

End: 940604

Strand: Direct

Name: pycB [H]

Synonym: EUBELI_00924

Alternate gene names: 238916855

Gene position: 939186-940604 (Clockwise)

Preceding gene: 238916854

Following gene: 238916856

Centisome position: 43.8

GC content: 39.82

Gene sequence:

>1419_bases
ATGGCAGAACAGGTTAAAAAACCTTTAAAGATTACTGAGACAGTATTGCGTGATGCACATCAGTCTCTTATAGCTACAAG
AATGACAACAGAGCAGATGCTTCCTATCGTAGATAAGATGGATAAGGTTGGTTATCATTCAGTAGAGTGCTGGGGAGGAG
CTACATTTGATGCTTCACTCAGATTCCTTAAGGAAGATCCATGGGAAAGACTTCGTAAGCTTAGAGATGGATTCAAGAAT
ACTAAGCTTCAGATGCTTTTCAGAGGACAGAATATATTAGGTTACAATCATTACGCAGATGATGTAGTTGAGTACTTTGT
TCAGAAGTCAATTGCTAACGGTATTGATATTATCCGTATTTTCGATTGCTTTAATGATTTAAGAAACTTAGAGACAGCTG
TTAAGGCTGCCAAGAAGGAAAAAGGACATGCTCAGATTGCTCTTTCTTATACATTAGGTGATGCTTATACACTTGATTAC
TGGAAAGAGACAGCTAAGAGAATTGAAGACATGGGTGCAGATTCTATCTGCATCAAGGATATGGCTGGACTTTTGCTTCC
ATACAAGGCTACAGAGCTTGTACAGGCATTAAAGGATGGTTCATCTCTTCCTATTCAGATGCATACACATTATACATCTG
GTGTAGCTTCAATGACATACTTAAAAGCAGTAGAAGCAGGTGCTGATATTATTGATACAGCTATGTCACCATTCTCAATG
GGAACATCACAGCCAGCTACAGAGGTTATGGTAGAGACATTTAAGGGTACACAGTATGATACAGGACTTGACCAGAATCT
TCTTGCTGAGATAGCTGATTACTTCCAGCCAATGCGTGAGGAAGCATTAAAGAGCGGACTTATGAATACTAAGGTTCTTG
GAGTTAATATTAAGACATTACTTTATCAGGTTCCAGGTGGAATGCTTTCTAACCTTGTATCACAGCTTAAGGAAGCTGGT
GCAGAGGACAAGTACAGAGATGTATTAGAGGAAATCCCAAGAGTTCGTAAAGATTATGGTGAACCACCTCTTGTTACTCC
TTCATCACAGATTGTTGGTACTCAGGCTGTTCTTAATGTATTACAGGGTGAGAGATACAAGATGATTACTAAGGAATCTA
AGAAGGTTCTTGCTGGTGAATTTGGTCAGACAATCAAGCCATTCAATCCAGAAGTTCAGAAGAAGGCTATCGGTGATACA
AAGCCTATTACATGCCGTCCAGCTGACTTGATTGAACCACAGCTTGAGAAATTCAAGAATGATCCAATTGTACAGCAGTA
CAAGCAGCAGGATGAGGATGTGCTTTCATATGCATTATTCCCACAGGTTGCGACAGAGTTCTTCAAGTATAGAGAAGCTC
AGCAGACAAAGGTTGATCCTACAAAGGCAGATACAGCTAATAAGGCTTATCCAGTCTAA

Upstream 100 bases:

>100_bases
GATGCATGCTATGGGACCTAACGTTGCCGGTGTTATTGGTACAGCGGTTGCAGCCGGTACATTTATGGCCATGTTTGGCG
TTATGTAAAAGGAGGATATT

Downstream 100 bases:

>100_bases
TAATTAATATAATAGACCGTCATGTGCGCAAAAGCGTATGTGGCGGTCTTTTTTTGCAGCGAAGCTGCGTAGAGACTTGA
CCTGCAAAAAAAGCAGGCAG

Product: oxaloacetate decarboxylase

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAEQVKKPLKITETVLRDAHQSLIATRMTTEQMLPIVDKMDKVGYHSVECWGGATFDASLRFLKEDPWERLRKLRDGFKN
TKLQMLFRGQNILGYNHYADDVVEYFVQKSIANGIDIIRIFDCFNDLRNLETAVKAAKKEKGHAQIALSYTLGDAYTLDY
WKETAKRIEDMGADSICIKDMAGLLLPYKATELVQALKDGSSLPIQMHTHYTSGVASMTYLKAVEAGADIIDTAMSPFSM
GTSQPATEVMVETFKGTQYDTGLDQNLLAEIADYFQPMREEALKSGLMNTKVLGVNIKTLLYQVPGGMLSNLVSQLKEAG
AEDKYRDVLEEIPRVRKDYGEPPLVTPSSQIVGTQAVLNVLQGERYKMITKESKKVLAGEFGQTIKPFNPEVQKKAIGDT
KPITCRPADLIEPQLEKFKNDPIVQQYKQQDEDVLSYALFPQVATEFFKYREAQQTKVDPTKADTANKAYPV

Sequences:

>Translated_472_residues
MAEQVKKPLKITETVLRDAHQSLIATRMTTEQMLPIVDKMDKVGYHSVECWGGATFDASLRFLKEDPWERLRKLRDGFKN
TKLQMLFRGQNILGYNHYADDVVEYFVQKSIANGIDIIRIFDCFNDLRNLETAVKAAKKEKGHAQIALSYTLGDAYTLDY
WKETAKRIEDMGADSICIKDMAGLLLPYKATELVQALKDGSSLPIQMHTHYTSGVASMTYLKAVEAGADIIDTAMSPFSM
GTSQPATEVMVETFKGTQYDTGLDQNLLAEIADYFQPMREEALKSGLMNTKVLGVNIKTLLYQVPGGMLSNLVSQLKEAG
AEDKYRDVLEEIPRVRKDYGEPPLVTPSSQIVGTQAVLNVLQGERYKMITKESKKVLAGEFGQTIKPFNPEVQKKAIGDT
KPITCRPADLIEPQLEKFKNDPIVQQYKQQDEDVLSYALFPQVATEFFKYREAQQTKVDPTKADTANKAYPV
>Mature_471_residues
AEQVKKPLKITETVLRDAHQSLIATRMTTEQMLPIVDKMDKVGYHSVECWGGATFDASLRFLKEDPWERLRKLRDGFKNT
KLQMLFRGQNILGYNHYADDVVEYFVQKSIANGIDIIRIFDCFNDLRNLETAVKAAKKEKGHAQIALSYTLGDAYTLDYW
KETAKRIEDMGADSICIKDMAGLLLPYKATELVQALKDGSSLPIQMHTHYTSGVASMTYLKAVEAGADIIDTAMSPFSMG
TSQPATEVMVETFKGTQYDTGLDQNLLAEIADYFQPMREEALKSGLMNTKVLGVNIKTLLYQVPGGMLSNLVSQLKEAGA
EDKYRDVLEEIPRVRKDYGEPPLVTPSSQIVGTQAVLNVLQGERYKMITKESKKVLAGEFGQTIKPFNPEVQKKAIGDTK
PITCRPADLIEPQLEKFKNDPIVQQYKQQDEDVLSYALFPQVATEFFKYREAQQTKVDPTKADTANKAYPV

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=461, Percent_Identity=33.6225596529284, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI106049295, Length=461, Percent_Identity=33.6225596529284, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI106049292, Length=461, Percent_Identity=33.6225596529284, Blast_Score=233, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17562816, Length=475, Percent_Identity=34.9473684210526, Blast_Score=243, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6321376, Length=477, Percent_Identity=33.7526205450734, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6319695, Length=473, Percent_Identity=34.0380549682875, Blast_Score=256, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24652212, Length=469, Percent_Identity=35.8208955223881, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24652210, Length=469, Percent_Identity=35.8208955223881, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24652214, Length=469, Percent_Identity=35.8208955223881, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI19921944, Length=469, Percent_Identity=35.8208955223881, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24652216, Length=469, Percent_Identity=35.8208955223881, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI281363050, Length=469, Percent_Identity=36.4605543710021, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24652224, Length=469, Percent_Identity=36.4605543710021, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24652222, Length=469, Percent_Identity=36.4605543710021, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24652220, Length=469, Percent_Identity=36.4605543710021, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24652218, Length=469, Percent_Identity=36.4605543710021, Blast_Score=260, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 53033; Mature: 52902

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQVKKPLKITETVLRDAHQSLIATRMTTEQMLPIVDKMDKVGYHSVECWGGATFDASL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHH
RFLKEDPWERLRKLRDGFKNTKLQMLFRGQNILGYNHYADDVVEYFVQKSIANGIDIIRI
HHHHCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
FDCFNDLRNLETAVKAAKKEKGHAQIALSYTLGDAYTLDYWKETAKRIEDMGADSICIKD
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEHHHHHHHHHHHHHCCCCHHHHHH
MAGLLLPYKATELVQALKDGSSLPIQMHTHYTSGVASMTYLKAVEAGADIIDTAMSPFSM
HHHHHCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCC
GTSQPATEVMVETFKGTQYDTGLDQNLLAEIADYFQPMREEALKSGLMNTKVLGVNIKTL
CCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHH
LYQVPGGMLSNLVSQLKEAGAEDKYRDVLEEIPRVRKDYGEPPLVTPSSQIVGTQAVLNV
HHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
LQGERYKMITKESKKVLAGEFGQTIKPFNPEVQKKAIGDTKPITCRPADLIEPQLEKFKN
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHCHHHHHHCC
DPIVQQYKQQDEDVLSYALFPQVATEFFKYREAQQTKVDPTKADTANKAYPV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AEQVKKPLKITETVLRDAHQSLIATRMTTEQMLPIVDKMDKVGYHSVECWGGATFDASL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHH
RFLKEDPWERLRKLRDGFKNTKLQMLFRGQNILGYNHYADDVVEYFVQKSIANGIDIIRI
HHHHCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
FDCFNDLRNLETAVKAAKKEKGHAQIALSYTLGDAYTLDYWKETAKRIEDMGADSICIKD
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEHHHHHHHHHHHHHCCCCHHHHHH
MAGLLLPYKATELVQALKDGSSLPIQMHTHYTSGVASMTYLKAVEAGADIIDTAMSPFSM
HHHHHCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCC
GTSQPATEVMVETFKGTQYDTGLDQNLLAEIADYFQPMREEALKSGLMNTKVLGVNIKTL
CCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHH
LYQVPGGMLSNLVSQLKEAGAEDKYRDVLEEIPRVRKDYGEPPLVTPSSQIVGTQAVLNV
HHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
LQGERYKMITKESKKVLAGEFGQTIKPFNPEVQKKAIGDTKPITCRPADLIEPQLEKFKN
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHCHHHHHHCC
DPIVQQYKQQDEDVLSYALFPQVATEFFKYREAQQTKVDPTKADTANKAYPV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]