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The map label for this gene is topA [H]

Identifier: 238916741

GI number: 238916741

Start: 819917

End: 821992

Strand: Direct

Name: topA [H]

Synonym: EUBELI_00803

Alternate gene names: 238916741

Gene position: 819917-821992 (Clockwise)

Preceding gene: 238916740

Following gene: 238916742

Centisome position: 38.24

GC content: 37.24

Gene sequence:

>2076_bases
ATGCATAAGTATTTGGTTATAGTGGAGTCACCTGCCAAGGTAAAGACTATCAGTAAATTCCTTGGAGCTAATTACAAGGT
AATGGCGTCACAGGGACATGTAAGGGATTTGCCTAAGAGCCAGATGGGAGTTGACATTGAACATGATTATGAACCTAAAT
ATATTACGATAAGGGGAAAGGGAGATATTCTTGCTGCTTTAAGAAAAGAAGCGAAGAAAGCTGATAAAGTATATCTCGCA
ACCGATCCCGACCGCGAGGGAGAAGCTATTTCATGGCATCTTGCAGCGGCACTTAAATTAGAAGATAAAGATATATACAG
AATTACATTTAACGAAATCACTAAGAATGCAGTTAAGGCATCATTGAAAGAAGCAAGAAAGATTGATATGAATCTTGTCG
ATGCTCAGCAGGCGAGAAGAGTATTAGACCGTGTTGTTGGTTATGGAATCAGTCCATTATTATGGGCTAAGATTAAAAGA
GGCTTAAGTGCTGGAAGAGTTCAGTCTGTTGCTTTAAGAATGATATGTGACAGAGAGAATGAGATAGATGAATTTATTCC
AGAAGAATACTGGTCTATGGAAGCTGTTCTTAATATTAAAGGGGAGAAAAAGCCTTTAATTGCAAAGTTCTATGGCGATG
AGAATGGAAAAATTGAAATTAAGAATGGTGCACAGATGCAGGATATTCTTGATGAAGTAAAGAAGAGTGATTTTAGTATT
GAAAGCATTAAGAGGGGAGAAAAGGTAAAGAAATCACCACTTCCATTTACTACAAGTACACTTCAGCAGGAAGCAGCTAA
AACTCTTAATATGTCTACAAAGCGTACAATGAATATTGCACAGCAGTTATATGAAGGCGTTGATATTAAAGGAAGAGGAA
CAGTAGGTCTTATTACGTATTTAAGAACAGATTCTACAAGAGTTGCAGATGAGGCTAAGGTATCATCCAGAGAATATATA
AGCCAGAATTATGGAGAAAAATATATTCCCCAAGAAGCTAATGCCAAGAAGGATGATAAGAAGATTCAGGATGCACATGA
GGCTATCAGACCAACTGATCTTAACTTATCACCGGCAATAGTAAAGGAATCTTTACAGAGAGATCAGTTCAGGCTTTATC
AGCTTATCTGGAAAAGATTTGTTGCAAGCCAGATGGCACCGGCTGTATATGAAACGACATCAGTAAAGATTATGGCAGGA
AAGTACAGATTCAGCGTAGCAGCTTCTAAGGTTATGTTTGATGGTTTTATGTCTGTATATAAGAATGATGATGACAATGA
AGAAAATAATACTCTTGCGAAAGGACTGGACGAGAATTCAGAACTTTCATTGGAAGATGTCAATGGAGTGCAGCATTTTA
CACAGCCACCTGCACATTTTACAGAAGCATCATTAGTAAAGGCGCTTGAGGAACAGGGAATAGGCAGACCAAGTACTTAT
GCTCCTACAATATCTACAATTATTGCAAGACATTATGTTATTAAAGAGAATAAGAATCTTTATATTTCAGAGCTTGGAAA
TGCGGTTAATAATATAATGATAACAGCTTTTCCTACTATTGTAGATGTTAAGTTTACAGCTAATATGGAGTCTCTTCTTG
ATGGCGTTGCAGAAGGAACCGTGGAATGGAAGGAAATTATAAGAAACTTTTATCCTGATCTTAAAGTTGCAATTGATGAA
GCGGAGAAGGAACTTGAACATGTTAAGATAGAAGATGAAGTAACTGATGTTATTTGTGATAAATGTGGCAGAAATATGGT
TATTAAGTATGGCCCTCATGGTAAGTTCTTAGGGTGTCCGGGATTTCCTGAATGTCATAATACCAAACCGTATCTTGAAA
AGATTGGAGTTCCATGTCCTAAGTGTGGAAAAGATGTTATCATAAAGAAAACAAAGAAGGGAAGAACCTTCTATGGATGT
GATGGCTACCCTGATTGTGATTTCGTATCATGGCAGAGACCGTCTGAGAAAAAATGTCCTAAGTGTGGCGGATATATGGT
TGAGAAAGGCAATAAAATTGTATGTGCTGATGAGACATGCGGCTATGTAGAGAATAAGAATGACAAGGAACAATAA

Upstream 100 bases:

>100_bases
GGCGGAGGGGATTCTGCTTGAGCTGGAGCTTGATGGGCTTATTGAAGAGGTTTCAAAGAATTATTATATAAGGAAGCATA
TTTGATTTGGAGGGCTATAG

Downstream 100 bases:

>100_bases
CCGGGTGGTTATTATCAGAAGAAGGATGGGAGATTTACAATGAGTGTTCAGCTTCTTGATAAAACAAGAAAAATCAATAA
GCTGCTCCACAATAATCATT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 691; Mature: 691

Protein sequence:

>691_residues
MHKYLVIVESPAKVKTISKFLGANYKVMASQGHVRDLPKSQMGVDIEHDYEPKYITIRGKGDILAALRKEAKKADKVYLA
TDPDREGEAISWHLAAALKLEDKDIYRITFNEITKNAVKASLKEARKIDMNLVDAQQARRVLDRVVGYGISPLLWAKIKR
GLSAGRVQSVALRMICDRENEIDEFIPEEYWSMEAVLNIKGEKKPLIAKFYGDENGKIEIKNGAQMQDILDEVKKSDFSI
ESIKRGEKVKKSPLPFTTSTLQQEAAKTLNMSTKRTMNIAQQLYEGVDIKGRGTVGLITYLRTDSTRVADEAKVSSREYI
SQNYGEKYIPQEANAKKDDKKIQDAHEAIRPTDLNLSPAIVKESLQRDQFRLYQLIWKRFVASQMAPAVYETTSVKIMAG
KYRFSVAASKVMFDGFMSVYKNDDDNEENNTLAKGLDENSELSLEDVNGVQHFTQPPAHFTEASLVKALEEQGIGRPSTY
APTISTIIARHYVIKENKNLYISELGNAVNNIMITAFPTIVDVKFTANMESLLDGVAEGTVEWKEIIRNFYPDLKVAIDE
AEKELEHVKIEDEVTDVICDKCGRNMVIKYGPHGKFLGCPGFPECHNTKPYLEKIGVPCPKCGKDVIIKKTKKGRTFYGC
DGYPDCDFVSWQRPSEKKCPKCGGYMVEKGNKIVCADETCGYVENKNDKEQ

Sequences:

>Translated_691_residues
MHKYLVIVESPAKVKTISKFLGANYKVMASQGHVRDLPKSQMGVDIEHDYEPKYITIRGKGDILAALRKEAKKADKVYLA
TDPDREGEAISWHLAAALKLEDKDIYRITFNEITKNAVKASLKEARKIDMNLVDAQQARRVLDRVVGYGISPLLWAKIKR
GLSAGRVQSVALRMICDRENEIDEFIPEEYWSMEAVLNIKGEKKPLIAKFYGDENGKIEIKNGAQMQDILDEVKKSDFSI
ESIKRGEKVKKSPLPFTTSTLQQEAAKTLNMSTKRTMNIAQQLYEGVDIKGRGTVGLITYLRTDSTRVADEAKVSSREYI
SQNYGEKYIPQEANAKKDDKKIQDAHEAIRPTDLNLSPAIVKESLQRDQFRLYQLIWKRFVASQMAPAVYETTSVKIMAG
KYRFSVAASKVMFDGFMSVYKNDDDNEENNTLAKGLDENSELSLEDVNGVQHFTQPPAHFTEASLVKALEEQGIGRPSTY
APTISTIIARHYVIKENKNLYISELGNAVNNIMITAFPTIVDVKFTANMESLLDGVAEGTVEWKEIIRNFYPDLKVAIDE
AEKELEHVKIEDEVTDVICDKCGRNMVIKYGPHGKFLGCPGFPECHNTKPYLEKIGVPCPKCGKDVIIKKTKKGRTFYGC
DGYPDCDFVSWQRPSEKKCPKCGGYMVEKGNKIVCADETCGYVENKNDKEQ
>Mature_691_residues
MHKYLVIVESPAKVKTISKFLGANYKVMASQGHVRDLPKSQMGVDIEHDYEPKYITIRGKGDILAALRKEAKKADKVYLA
TDPDREGEAISWHLAAALKLEDKDIYRITFNEITKNAVKASLKEARKIDMNLVDAQQARRVLDRVVGYGISPLLWAKIKR
GLSAGRVQSVALRMICDRENEIDEFIPEEYWSMEAVLNIKGEKKPLIAKFYGDENGKIEIKNGAQMQDILDEVKKSDFSI
ESIKRGEKVKKSPLPFTTSTLQQEAAKTLNMSTKRTMNIAQQLYEGVDIKGRGTVGLITYLRTDSTRVADEAKVSSREYI
SQNYGEKYIPQEANAKKDDKKIQDAHEAIRPTDLNLSPAIVKESLQRDQFRLYQLIWKRFVASQMAPAVYETTSVKIMAG
KYRFSVAASKVMFDGFMSVYKNDDDNEENNTLAKGLDENSELSLEDVNGVQHFTQPPAHFTEASLVKALEEQGIGRPSTY
APTISTIIARHYVIKENKNLYISELGNAVNNIMITAFPTIVDVKFTANMESLLDGVAEGTVEWKEIIRNFYPDLKVAIDE
AEKELEHVKIEDEVTDVICDKCGRNMVIKYGPHGKFLGCPGFPECHNTKPYLEKIGVPCPKCGKDVIIKKTKKGRTFYGC
DGYPDCDFVSWQRPSEKKCPKCGGYMVEKGNKIVCADETCGYVENKNDKEQ

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=590, Percent_Identity=24.406779661017, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI4507635, Length=512, Percent_Identity=23.6328125, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI1787529, Length=749, Percent_Identity=38.7182910547397, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI1788061, Length=560, Percent_Identity=24.6428571428571, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI48994931, Length=114, Percent_Identity=35.0877192982456, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32563869, Length=714, Percent_Identity=25.9103641456583, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17555378, Length=524, Percent_Identity=23.8549618320611, Blast_Score=129, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6323263, Length=484, Percent_Identity=22.7272727272727, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24585251, Length=659, Percent_Identity=24.5827010622155, Blast_Score=139, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24640096, Length=638, Percent_Identity=24.294670846395, Blast_Score=133, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 77883; Mature: 77883

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKYLVIVESPAKVKTISKFLGANYKVMASQGHVRDLPKSQMGVDIEHDYEPKYITIRGK
CCEEEEEEECCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCEEEEECC
GDILAALRKEAKKADKVYLATDPDREGEAISWHLAAALKLEDKDIYRITFNEITKNAVKA
CHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEEEEEEECCCCEEEEEHHHHHHHHHHH
SLKEARKIDMNLVDAQQARRVLDRVVGYGISPLLWAKIKRGLSAGRVQSVALRMICDREN
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
EIDEFIPEEYWSMEAVLNIKGEKKPLIAKFYGDENGKIEIKNGAQMQDILDEVKKSDFSI
CHHHHCHHHHHCEEEEEECCCCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHCCCCH
ESIKRGEKVKKSPLPFTTSTLQQEAAKTLNMSTKRTMNIAQQLYEGVDIKGRGTVGLITY
HHHHCCCHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEE
LRTDSTRVADEAKVSSREYISQNYGEKYIPQEANAKKDDKKIQDAHEAIRPTDLNLSPAI
EECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHH
VKESLQRDQFRLYQLIWKRFVASQMAPAVYETTSVKIMAGKYRFSVAASKVMFDGFMSVY
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCEEEEEECCEEEHHHHHHHHHHHHHHHH
KNDDDNEENNTLAKGLDENSELSLEDVNGVQHFTQPPAHFTEASLVKALEEQGIGRPSTY
CCCCCCCCCCHHHHCCCCCCCCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCC
APTISTIIARHYVIKENKNLYISELGNAVNNIMITAFPTIVDVKFTANMESLLDGVAEGT
CHHHHHHHHHHHEEECCCCEEHHHHHHHHHCEEEEEECEEEEEEECCCHHHHHHHHHHCC
VEWKEIIRNFYPDLKVAIDEAEKELEHVKIEDEVTDVICDKCGRNMVIKYGPHGKFLGCP
HHHHHHHHHHCCCCEEHHHHHHHHHHHEECHHHHHHHHHHHCCCCCEEEECCCCCEECCC
GFPECHNTKPYLEKIGVPCPKCGKDVIIKKTKKGRTFYGCDGYPDCDFVSWQRPSEKKCP
CCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCC
KCGGYMVEKGNKIVCADETCGYVENKNDKEQ
CCCCEEEECCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHKYLVIVESPAKVKTISKFLGANYKVMASQGHVRDLPKSQMGVDIEHDYEPKYITIRGK
CCEEEEEEECCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCEEEEECC
GDILAALRKEAKKADKVYLATDPDREGEAISWHLAAALKLEDKDIYRITFNEITKNAVKA
CHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEEEEEEECCCCEEEEEHHHHHHHHHHH
SLKEARKIDMNLVDAQQARRVLDRVVGYGISPLLWAKIKRGLSAGRVQSVALRMICDREN
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
EIDEFIPEEYWSMEAVLNIKGEKKPLIAKFYGDENGKIEIKNGAQMQDILDEVKKSDFSI
CHHHHCHHHHHCEEEEEECCCCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHCCCCH
ESIKRGEKVKKSPLPFTTSTLQQEAAKTLNMSTKRTMNIAQQLYEGVDIKGRGTVGLITY
HHHHCCCHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEE
LRTDSTRVADEAKVSSREYISQNYGEKYIPQEANAKKDDKKIQDAHEAIRPTDLNLSPAI
EECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHH
VKESLQRDQFRLYQLIWKRFVASQMAPAVYETTSVKIMAGKYRFSVAASKVMFDGFMSVY
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCEEEEEECCEEEHHHHHHHHHHHHHHHH
KNDDDNEENNTLAKGLDENSELSLEDVNGVQHFTQPPAHFTEASLVKALEEQGIGRPSTY
CCCCCCCCCCHHHHCCCCCCCCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCC
APTISTIIARHYVIKENKNLYISELGNAVNNIMITAFPTIVDVKFTANMESLLDGVAEGT
CHHHHHHHHHHHEEECCCCEEHHHHHHHHHCEEEEEECEEEEEEECCCHHHHHHHHHHCC
VEWKEIIRNFYPDLKVAIDEAEKELEHVKIEDEVTDVICDKCGRNMVIKYGPHGKFLGCP
HHHHHHHHHHCCCCEEHHHHHHHHHHHEECHHHHHHHHHHHCCCCCEEEECCCCCEECCC
GFPECHNTKPYLEKIGVPCPKCGKDVIIKKTKKGRTFYGCDGYPDCDFVSWQRPSEKKCP
CCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCC
KCGGYMVEKGNKIVCADETCGYVENKNDKEQ
CCCCEEEECCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]