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The map label for this gene is sigK [H]

Identifier: 238916719

GI number: 238916719

Start: 790637

End: 791308

Strand: Direct

Name: sigK [H]

Synonym: EUBELI_00781

Alternate gene names: 238916719

Gene position: 790637-791308 (Clockwise)

Preceding gene: 238916718

Following gene: 238916720

Centisome position: 36.87

GC content: 36.61

Gene sequence:

>672_bases
ATGTGTAGGGGTGCCTTTTTGAAAACATTTGAAAAACCATTATCGGCACAGGAAGAGAAAGAATTGCTGATAAAATTACG
CGAAGGCGATAGTGCGGCCAGAAATGCTCTTATAGAAAAGAACATGCGTCTTGTTGCACATATAGTCAAAAAATATACCT
CTACTGAACGTGATTATATTAATGAAGACCTTATTTCAGTGGGAACAATAGGACTTATTAAGGCTGTTGATTCATTTGAC
ATAGAAAGAAATGTGAGATTCTCAACATATGCTGCCAAATGTATAGACAACGAAATTCTTATGCTTTTCCGTCAGGATAA
AAAGAAGGAACGGGAAGTGTCTCTTAATGAGCCTATAGGAAAGGACAAAGAGGGAAATGAGATAAGCCTTAAGGATATTA
TCGGAGAAGATTCGGAGAAGAAACAGCCTGATGCTGTGGAAGATTATATGTTTTATGAGCAGATTAAGTGTATATATGGT
AATATAGAAAAGGTGCTTGCACCAAGAGAGATAGAGATATTAAAATACAGGTATGGACTTTTTGGTGCAAAGGAATGTAC
ACAGAATGAACTTGCTGACAGGCTTGGAATAAGCAGAAGCTATGTGAGCAGAATAGAGAAAAAGGCACTTGGTAAATTAA
GAGAAGTGCTTATTGAGTGGAAACTTATATGA

Upstream 100 bases:

>100_bases
ATCTTAAGACGTCAGGAAGAAGAAACAGAAGTTAACAGTTAAATTAAAGAGACAGTTGGATAGGCAATTAAAGGCACCTT
TACACATATGATATATAAAA

Downstream 100 bases:

>100_bases
TAATATAGTCAGGAGGACATGAAATGCAGATTACAGATGCAAAGGTAATAGCAGGCAGATTATCTAAGGTAAGGGATATC
ATGAAATCAGAAGGCGTTGA

Product: RNA polymerase sporulation-specific sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MCRGAFLKTFEKPLSAQEEKELLIKLREGDSAARNALIEKNMRLVAHIVKKYTSTERDYINEDLISVGTIGLIKAVDSFD
IERNVRFSTYAAKCIDNEILMLFRQDKKKEREVSLNEPIGKDKEGNEISLKDIIGEDSEKKQPDAVEDYMFYEQIKCIYG
NIEKVLAPREIEILKYRYGLFGAKECTQNELADRLGISRSYVSRIEKKALGKLREVLIEWKLI

Sequences:

>Translated_223_residues
MCRGAFLKTFEKPLSAQEEKELLIKLREGDSAARNALIEKNMRLVAHIVKKYTSTERDYINEDLISVGTIGLIKAVDSFD
IERNVRFSTYAAKCIDNEILMLFRQDKKKEREVSLNEPIGKDKEGNEISLKDIIGEDSEKKQPDAVEDYMFYEQIKCIYG
NIEKVLAPREIEILKYRYGLFGAKECTQNELADRLGISRSYVSRIEKKALGKLREVLIEWKLI
>Mature_223_residues
MCRGAFLKTFEKPLSAQEEKELLIKLREGDSAARNALIEKNMRLVAHIVKKYTSTERDYINEDLISVGTIGLIKAVDSFD
IERNVRFSTYAAKCIDNEILMLFRQDKKKEREVSLNEPIGKDKEGNEISLKDIIGEDSEKKQPDAVEDYMFYEQIKCIYG
NIEKVLAPREIEILKYRYGLFGAKECTQNELADRLGISRSYVSRIEKKALGKLREVLIEWKLI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=257, Percent_Identity=27.6264591439689, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1789448, Length=252, Percent_Identity=26.5873015873016, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014209
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 25778; Mature: 25778

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRGAFLKTFEKPLSAQEEKELLIKLREGDSAARNALIEKNMRLVAHIVKKYTSTERDYI
CCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
NEDLISVGTIGLIKAVDSFDIERNVRFSTYAAKCIDNEILMLFRQDKKKEREVSLNEPIG
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KDKEGNEISLKDIIGEDSEKKQPDAVEDYMFYEQIKCIYGNIEKVLAPREIEILKYRYGL
CCCCCCEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHCC
FGAKECTQNELADRLGISRSYVSRIEKKALGKLREVLIEWKLI
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCRGAFLKTFEKPLSAQEEKELLIKLREGDSAARNALIEKNMRLVAHIVKKYTSTERDYI
CCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
NEDLISVGTIGLIKAVDSFDIERNVRFSTYAAKCIDNEILMLFRQDKKKEREVSLNEPIG
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KDKEGNEISLKDIIGEDSEKKQPDAVEDYMFYEQIKCIYGNIEKVLAPREIEILKYRYGL
CCCCCCEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHCC
FGAKECTQNELADRLGISRSYVSRIEKKALGKLREVLIEWKLI
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]