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The map label for this gene is hemB [H]

Identifier: 238916683

GI number: 238916683

Start: 754905

End: 755972

Strand: Direct

Name: hemB [H]

Synonym: EUBELI_00745

Alternate gene names: 238916683

Gene position: 754905-755972 (Clockwise)

Preceding gene: 238916682

Following gene: 238916684

Centisome position: 35.21

GC content: 41.48

Gene sequence:

>1068_bases
ATGCAAGAATGGTTAAGATACATAGCAGAACATAACATACCAGTTTACAATGTTTATATGCAATGCTATTATATAGGAAA
CGTTGGAAGGAAGATGATAATCATGGACAGAACAAGAAGATTAAGAATGAATGCGACATTAAGAGATATGGTAAGAGAGA
ATCATGTAAGGGTGGACGAGCTTATATATCCTGTATTTGTTACGGAGGAGAACGATGTAATACAGGAAGTGCCGTCAATG
CCGGGAATATGTCAGTATTCGCTTGACCATGTTCTTGAAGAAATCGGACGTGCTGTAGAGGTTGGAATTAAGGGAATTCT
GCTTTTTGGAATACCTGTACATAAAGATGAGGTCGGAAGTGAGGCGTATGACGAGAAAGGTGTTGTGTGCAGGGCAATCC
GCCTTATTAAGGATGCTTATCCAGAGCTTGTTATCATGGCTGATGTGTGCCTGTGTGAGTACACATCACATGGACACTGC
GGACTTGTAAAAGATGGCGTTATATTAAATGATGAGACACTGCCGCTTCTTGCAAAGGCGTCAGTTGCTTATGCAAAAGC
GGGGGCAGATATAATTGCACCGAGTGACATGATGGATAAGAGAGTTGAGGCAATCAGAAATGCACTTGATGAGGCAGGAC
TGGTTAATATTCCTATATGTTCATACAGTGCCAAATTTGCATCAGGATATTACGGACCGTTCAGGGATGCCGCACATTCA
GCACCGGAATTTGGTGACAGAAAGACATATCAGATGGATCCGGCTAATGGAAAAGAAGCGTTAAGGGAAGTTGAAGAAGA
CATATTAGAAGGTGCAGACATGATTATTGCAAAGCCGGCCCTCGGATATATGGACGTTATGAAAGAGATAGCGCTTAACT
TCAATATTCCAATTGTCGCTTATAATGTAAGCGGTGAGTACGCGATGGTTAAGGCAGCAGCAATGAATGGCTGGATTGAT
GAGAAGAAGATTGTAATGGAGAATATGACAGGCTTTAAGAGAGCCGGAGCTAAGATGATAATTACATATCATGCTATAGA
TGTGGCAAAATGGTTAAAGGAAGAGTAA

Upstream 100 bases:

>100_bases
CTTACAACTATTGAAAATGCGGAGGAAATCCTGATGCTTACTGACGAGGGCATCAAGGAGAGAGGAACTCACGAAGAACT
TATGAAGCTTGATGGTGAGT

Downstream 100 bases:

>100_bases
CATTTTTAGCAGTATTAAATTAAGCAGGAGGAGCTGGTTTTATGAATGAAGAGATGTCAAATGATTTATATGAGAGAGCA
GTAAAACATATGCCGGGTGG

Product: porphobilinogen synthase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MQEWLRYIAEHNIPVYNVYMQCYYIGNVGRKMIIMDRTRRLRMNATLRDMVRENHVRVDELIYPVFVTEENDVIQEVPSM
PGICQYSLDHVLEEIGRAVEVGIKGILLFGIPVHKDEVGSEAYDEKGVVCRAIRLIKDAYPELVIMADVCLCEYTSHGHC
GLVKDGVILNDETLPLLAKASVAYAKAGADIIAPSDMMDKRVEAIRNALDEAGLVNIPICSYSAKFASGYYGPFRDAAHS
APEFGDRKTYQMDPANGKEALREVEEDILEGADMIIAKPALGYMDVMKEIALNFNIPIVAYNVSGEYAMVKAAAMNGWID
EKKIVMENMTGFKRAGAKMIITYHAIDVAKWLKEE

Sequences:

>Translated_355_residues
MQEWLRYIAEHNIPVYNVYMQCYYIGNVGRKMIIMDRTRRLRMNATLRDMVRENHVRVDELIYPVFVTEENDVIQEVPSM
PGICQYSLDHVLEEIGRAVEVGIKGILLFGIPVHKDEVGSEAYDEKGVVCRAIRLIKDAYPELVIMADVCLCEYTSHGHC
GLVKDGVILNDETLPLLAKASVAYAKAGADIIAPSDMMDKRVEAIRNALDEAGLVNIPICSYSAKFASGYYGPFRDAAHS
APEFGDRKTYQMDPANGKEALREVEEDILEGADMIIAKPALGYMDVMKEIALNFNIPIVAYNVSGEYAMVKAAAMNGWID
EKKIVMENMTGFKRAGAKMIITYHAIDVAKWLKEE
>Mature_355_residues
MQEWLRYIAEHNIPVYNVYMQCYYIGNVGRKMIIMDRTRRLRMNATLRDMVRENHVRVDELIYPVFVTEENDVIQEVPSM
PGICQYSLDHVLEEIGRAVEVGIKGILLFGIPVHKDEVGSEAYDEKGVVCRAIRLIKDAYPELVIMADVCLCEYTSHGHC
GLVKDGVILNDETLPLLAKASVAYAKAGADIIAPSDMMDKRVEAIRNALDEAGLVNIPICSYSAKFASGYYGPFRDAAHS
APEFGDRKTYQMDPANGKEALREVEEDILEGADMIIAKPALGYMDVMKEIALNFNIPIVAYNVSGEYAMVKAAAMNGWID
EKKIVMENMTGFKRAGAKMIITYHAIDVAKWLKEE

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=305, Percent_Identity=47.8688524590164, Blast_Score=291, Evalue=8e-79,
Organism=Escherichia coli, GI87081728, Length=317, Percent_Identity=48.5804416403785, Blast_Score=303, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6321398, Length=315, Percent_Identity=45.3968253968254, Blast_Score=269, Evalue=6e-73,
Organism=Drosophila melanogaster, GI21358291, Length=305, Percent_Identity=43.2786885245902, Blast_Score=264, Evalue=6e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 39818; Mature: 39818

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEWLRYIAEHNIPVYNVYMQCYYIGNVGRKMIIMDRTRRLRMNATLRDMVRENHVRVDE
CHHHHHHHHHCCCCEEEEEEEEEEECCCCCEEEEEECCHHHECCHHHHHHHHHCCCCHHH
LIYPVFVTEENDVIQEVPSMPGICQYSLDHVLEEIGRAVEVGIKGILLFGIPVHKDEVGS
EEEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCC
EAYDEKGVVCRAIRLIKDAYPELVIMADVCLCEYTSHGHCGLVKDGVILNDETLPLLAKA
HHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCEEECCEEECCCCCCHHHHH
SVAYAKAGADIIAPSDMMDKRVEAIRNALDEAGLVNIPICSYSAKFASGYYGPFRDAAHS
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHCCCCCCHHHHHCC
APEFGDRKTYQMDPANGKEALREVEEDILEGADMIIAKPALGYMDVMKEIALNFNIPIVA
CCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEE
YNVSGEYAMVKAAAMNGWIDEKKIVMENMTGFKRAGAKMIITYHAIDVAKWLKEE
EECCCCEEEEEHHHHCCCCCHHHHHHHHCCHHHHCCCEEEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MQEWLRYIAEHNIPVYNVYMQCYYIGNVGRKMIIMDRTRRLRMNATLRDMVRENHVRVDE
CHHHHHHHHHCCCCEEEEEEEEEEECCCCCEEEEEECCHHHECCHHHHHHHHHCCCCHHH
LIYPVFVTEENDVIQEVPSMPGICQYSLDHVLEEIGRAVEVGIKGILLFGIPVHKDEVGS
EEEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCC
EAYDEKGVVCRAIRLIKDAYPELVIMADVCLCEYTSHGHCGLVKDGVILNDETLPLLAKA
HHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCEEECCEEECCCCCCHHHHH
SVAYAKAGADIIAPSDMMDKRVEAIRNALDEAGLVNIPICSYSAKFASGYYGPFRDAAHS
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHCCCCCCHHHHHCC
APEFGDRKTYQMDPANGKEALREVEEDILEGADMIIAKPALGYMDVMKEIALNFNIPIVA
CCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEE
YNVSGEYAMVKAAAMNGWIDEKKIVMENMTGFKRAGAKMIITYHAIDVAKWLKEE
EECCCCEEEEEHHHHCCCCCHHHHHHHHCCHHHHCCCEEEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]