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The map label for this gene is hemL2 [H]

Identifier: 238916684

GI number: 238916684

Start: 756014

End: 757288

Strand: Direct

Name: hemL2 [H]

Synonym: EUBELI_00746

Alternate gene names: 238916684

Gene position: 756014-757288 (Clockwise)

Preceding gene: 238916683

Following gene: 238916685

Centisome position: 35.26

GC content: 42.04

Gene sequence:

>1275_bases
ATGAATGAAGAGATGTCAAATGATTTATATGAGAGAGCAGTAAAACATATGCCGGGTGGTGTAAACAGCCCTGTCAGGGC
ATACAAGGCAGTTGACAGAGTGCCAAGATTCATAGCTTCTGCCAAGGGAGACAGAATTACTGATGTAGATGGGAATGAAA
TGATTGACTACATATGTTCATGGGGACCGGGAATATTAGGCCATGCACATGACAGAGTAATTGCAAAGGTTAAGGAAGCT
GCGGAAGCAGGCCTTACATATGGTGCACCAACCAAGAGAGAAGTTGAGATGGCAGAGCTTATATATGAGCTTATTCCTAC
AATGGAGGTGAGCCGTCTTGTAAGTTCAGGAACAGAAGCCGTTATGAGCGCAATCCGTGTGGCAAGAGGCTACACCAGAA
GAGATAAGATAATTAAGTTCAGAGGCTGTTATCATGGACATTCTGACGGACTTTTAGTAAAAGCCGGTTCAGCAGCACTT
ACAACATCAGTTCCGGACAGTGCAGGAGTTCCTGCGGATTACACTAAGAATACACTTGTAGCAGAGTACAATGACTGCGA
TTCTGTGAAAGAGCTTTTTGATAACAATAAAGATGAAATAGCTGCGGTAATAGTCGAACCAGTTGCAGCTAACATGGGAG
TTGTGCTTCCAAGACATGGATTTCTTGAATTCTTAAGAGACATAACTAAGGAAAATGGAAGTCTTCTTATATTTGATGAG
GTAATAACAGGCTTCAGACTTTCAATTGGCGGAGCACAGGAGTACTTTAACATAAAGCCAGATCTTACAACTCTTGGAAA
GATTGTAGGTGGGGGTATGCCAGTCGGCGCATATGGCGGACGAGCTGACATTATGCGAATGGTAAGTCCTGACGGTCCTG
TATATCAGGCAGGAACATTGTCAGGCAATCCAATTGCAACAGCAGCAGGCTTAGAGACTTTAAGAATCTTAAGAGATAAT
AAGGACATATACGACAGACTTGAGACTAAGACAAAGCGTATTGCTGACAGTGTAAGAAAATGTGGAGCCGGAAGGGTATC
GGTTAATCAGATTGGTTCACTTATGAGTATATTCTTTACACCAGATGCAGTTGAAGATTATGACAGTGCAGTAAAGTCAG
ACACTAAGAAGTATGCAGAATACTTTGGACATATGCTTGATAACGGAATATACATTGCACCATCACAGTTTGAGGCAATG
TTCGTATCAGACGCACACACTAATGAGGATATCAACCGTACAATAGAGGTTATGGAAGAATTCTTCAGGAAATAA

Upstream 100 bases:

>100_bases
CTAAGATGATAATTACATATCATGCTATAGATGTGGCAAAATGGTTAAAGGAAGAGTAACATTTTTAGCAGTATTAAATT
AAGCAGGAGGAGCTGGTTTT

Downstream 100 bases:

>100_bases
TTCGGATAATAAGTGGGAATACAGATGCAGTTAGGATATGTAGGAATTAATTATAAGAATACAGACCTTTCAGTAAGGGA
CAAGATTACATTCACAGACA

Product: glutamate-1-semialdehyde 2,1-aminomutase

Products: NA

Alternate protein names: GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2 [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MNEEMSNDLYERAVKHMPGGVNSPVRAYKAVDRVPRFIASAKGDRITDVDGNEMIDYICSWGPGILGHAHDRVIAKVKEA
AEAGLTYGAPTKREVEMAELIYELIPTMEVSRLVSSGTEAVMSAIRVARGYTRRDKIIKFRGCYHGHSDGLLVKAGSAAL
TTSVPDSAGVPADYTKNTLVAEYNDCDSVKELFDNNKDEIAAVIVEPVAANMGVVLPRHGFLEFLRDITKENGSLLIFDE
VITGFRLSIGGAQEYFNIKPDLTTLGKIVGGGMPVGAYGGRADIMRMVSPDGPVYQAGTLSGNPIATAAGLETLRILRDN
KDIYDRLETKTKRIADSVRKCGAGRVSVNQIGSLMSIFFTPDAVEDYDSAVKSDTKKYAEYFGHMLDNGIYIAPSQFEAM
FVSDAHTNEDINRTIEVMEEFFRK

Sequences:

>Translated_424_residues
MNEEMSNDLYERAVKHMPGGVNSPVRAYKAVDRVPRFIASAKGDRITDVDGNEMIDYICSWGPGILGHAHDRVIAKVKEA
AEAGLTYGAPTKREVEMAELIYELIPTMEVSRLVSSGTEAVMSAIRVARGYTRRDKIIKFRGCYHGHSDGLLVKAGSAAL
TTSVPDSAGVPADYTKNTLVAEYNDCDSVKELFDNNKDEIAAVIVEPVAANMGVVLPRHGFLEFLRDITKENGSLLIFDE
VITGFRLSIGGAQEYFNIKPDLTTLGKIVGGGMPVGAYGGRADIMRMVSPDGPVYQAGTLSGNPIATAAGLETLRILRDN
KDIYDRLETKTKRIADSVRKCGAGRVSVNQIGSLMSIFFTPDAVEDYDSAVKSDTKKYAEYFGHMLDNGIYIAPSQFEAM
FVSDAHTNEDINRTIEVMEEFFRK
>Mature_424_residues
MNEEMSNDLYERAVKHMPGGVNSPVRAYKAVDRVPRFIASAKGDRITDVDGNEMIDYICSWGPGILGHAHDRVIAKVKEA
AEAGLTYGAPTKREVEMAELIYELIPTMEVSRLVSSGTEAVMSAIRVARGYTRRDKIIKFRGCYHGHSDGLLVKAGSAAL
TTSVPDSAGVPADYTKNTLVAEYNDCDSVKELFDNNKDEIAAVIVEPVAANMGVVLPRHGFLEFLRDITKENGSLLIFDE
VITGFRLSIGGAQEYFNIKPDLTTLGKIVGGGMPVGAYGGRADIMRMVSPDGPVYQAGTLSGNPIATAAGLETLRILRDN
KDIYDRLETKTKRIADSVRKCGAGRVSVNQIGSLMSIFFTPDAVEDYDSAVKSDTKKYAEYFGHMLDNGIYIAPSQFEAM
FVSDAHTNEDINRTIEVMEEFFRK

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=321, Percent_Identity=28.0373831775701, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI13994255, Length=321, Percent_Identity=26.1682242990654, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI24119277, Length=319, Percent_Identity=25.3918495297806, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI37574042, Length=355, Percent_Identity=24.7887323943662, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI226442709, Length=324, Percent_Identity=25, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI226442705, Length=354, Percent_Identity=24.5762711864407, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI284507298, Length=211, Percent_Identity=29.8578199052133, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786349, Length=423, Percent_Identity=53.4278959810875, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1789016, Length=422, Percent_Identity=30.5687203791469, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787560, Length=400, Percent_Identity=27, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI145693181, Length=309, Percent_Identity=29.7734627831715, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788044, Length=292, Percent_Identity=31.1643835616438, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1789759, Length=309, Percent_Identity=29.126213592233, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786991, Length=320, Percent_Identity=28.125, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI25144271, Length=354, Percent_Identity=27.1186440677966, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71992977, Length=363, Percent_Identity=23.6914600550964, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144274, Length=195, Percent_Identity=30.2564102564103, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=391, Percent_Identity=24.2966751918159, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324432, Length=309, Percent_Identity=25.8899676375405, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21357415, Length=301, Percent_Identity=27.2425249169435, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46408; Mature: 46408

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEEMSNDLYERAVKHMPGGVNSPVRAYKAVDRVPRFIASAKGDRITDVDGNEMIDYICS
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHH
WGPGILGHAHDRVIAKVKEAAEAGLTYGAPTKREVEMAELIYELIPTMEVSRLVSSGTEA
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCHHH
VMSAIRVARGYTRRDKIIKFRGCYHGHSDGLLVKAGSAALTTSVPDSAGVPADYTKNTLV
HHHHHHHHHCCCHHHHEEEEECCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCCEE
AEYNDCDSVKELFDNNKDEIAAVIVEPVAANMGVVLPRHGFLEFLRDITKENGSLLIFDE
EECCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCCEEEEHH
VITGFRLSIGGAQEYFNIKPDLTTLGKIVGGGMPVGAYGGRADIMRMVSPDGPVYQAGTL
HHCCCEEEECCCHHHEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEECCCC
SGNPIATAAGLETLRILRDNKDIYDRLETKTKRIADSVRKCGAGRVSVNQIGSLMSIFFT
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
PDAVEDYDSAVKSDTKKYAEYFGHMLDNGIYIAPSQFEAMFVSDAHTNEDINRTIEVMEE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHH
FFRK
HHCC
>Mature Secondary Structure
MNEEMSNDLYERAVKHMPGGVNSPVRAYKAVDRVPRFIASAKGDRITDVDGNEMIDYICS
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHH
WGPGILGHAHDRVIAKVKEAAEAGLTYGAPTKREVEMAELIYELIPTMEVSRLVSSGTEA
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCHHH
VMSAIRVARGYTRRDKIIKFRGCYHGHSDGLLVKAGSAALTTSVPDSAGVPADYTKNTLV
HHHHHHHHHCCCHHHHEEEEECCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCCEE
AEYNDCDSVKELFDNNKDEIAAVIVEPVAANMGVVLPRHGFLEFLRDITKENGSLLIFDE
EECCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCCEEEEHH
VITGFRLSIGGAQEYFNIKPDLTTLGKIVGGGMPVGAYGGRADIMRMVSPDGPVYQAGTL
HHCCCEEEECCCHHHEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEECCCC
SGNPIATAAGLETLRILRDNKDIYDRLETKTKRIADSVRKCGAGRVSVNQIGSLMSIFFT
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
PDAVEDYDSAVKSDTKKYAEYFGHMLDNGIYIAPSQFEAMFVSDAHTNEDINRTIEVMEE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHH
FFRK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA