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The map label for this gene is rlmN

Identifier: 238916657

GI number: 238916657

Start: 722259

End: 723311

Strand: Direct

Name: rlmN

Synonym: EUBELI_00719

Alternate gene names: 238916657

Gene position: 722259-723311 (Clockwise)

Preceding gene: 238916656

Following gene: 238916658

Centisome position: 33.68

GC content: 38.18

Gene sequence:

>1053_bases
ATGACAGATATTAAGTCACTTAATTATGACGAACTTGTAACATATATGGCCGGACTTGGTGAGAAGAAGTTCAGGGCTGG
GCAGCTATATCAGTGGATGCATGAAAAGCTGGCAGACAGCTTTGATGAATGTACCAATCTTTCCAATGCATTAAGGCAGA
AGTTAAAGGAAACATCAGAATATGTATGTCTTGAGCCTGTCAGGGTACAGCATTCAAAGCTTGACGGAACAGAAAAATAT
CTGTTCAGATTATCAGATGGTAATTATGTTGAAAGCGTACTCATGAAATATCATCATGGTAATTCAGTGTGTATATCTTC
ACAGGTAGGCTGCCGTATGGGCTGCCGTTTCTGTGCATCTACACTTAATGGAAAAGTCAGGGATTTAAGACCATCTGAAA
TGCTTGACCAGATATACAGAATACAGAAGATTACAGGGGAGCGAGTAAGCAATGTTGTAGTTATGGGTTCAGGAGAGCCA
ATGGATAACTATGATAATCTTATTAAGTTCATAGAACTTCTCAATGACGAGAGGGGGCTTAACATAAGCCAGCGTAATAT
TACTGTATCATCATGTGGAATAGTACCTAAGCTAAAGGAACTTGCTGACCTTAAGCTGCAGATTACTCTTGCTATTTCAC
TTCATGCTCCAAATGATGAACTAAGAAAGACAATGATGCCTATAGCTAATAAATATTCAATAGAAGAGATAATGGATGTA
TGCAGGTATTACATAGAATGTACAGGCAGAAGAATCTCATTTGAATACAGTCTTGTTAAAGGCGTCAATGATTCAATGGA
ATGTGCAAAGCAGTTGATTGAACTTGTTAAGGGCATGAATTGCCATATTAATCTTATACCTGTTAATCCTATCAAGGAGC
GTGATTATAAGCAGACAGGTAAGGAGGAAGTGTACGCATTTAAAAATAAACTTGAAAAGAACGGAATAAATGTTACAATT
AGAAGAGAAATGGGCAGAGATATTGATGGTGCCTGTGGACAGTTAAGGAATAAGTATATGGAGGATATCGATGAGAGCGA
CGGCGGTTACTGA

Upstream 100 bases:

>100_bases
AAGAATATGGCTTTAAGAAGATAGAATACAGACAGATTCTGCCGGGAAAGAATGGCGGAACAGATGGGTTCTTTGTCGCA
AAACTTAGGAGAGAATAGAC

Downstream 100 bases:

>100_bases
TATTGGCAAAACTCGTGCCGTTAATCAGGATTATGTATATACATCAGTTGACAGTATTGGATGTCTGCCTAATCTTTTTA
TTGTTGCAGATGGAATGGGA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MTDIKSLNYDELVTYMAGLGEKKFRAGQLYQWMHEKLADSFDECTNLSNALRQKLKETSEYVCLEPVRVQHSKLDGTEKY
LFRLSDGNYVESVLMKYHHGNSVCISSQVGCRMGCRFCASTLNGKVRDLRPSEMLDQIYRIQKITGERVSNVVVMGSGEP
MDNYDNLIKFIELLNDERGLNISQRNITVSSCGIVPKLKELADLKLQITLAISLHAPNDELRKTMMPIANKYSIEEIMDV
CRYYIECTGRRISFEYSLVKGVNDSMECAKQLIELVKGMNCHINLIPVNPIKERDYKQTGKEEVYAFKNKLEKNGINVTI
RREMGRDIDGACGQLRNKYMEDIDESDGGY

Sequences:

>Translated_350_residues
MTDIKSLNYDELVTYMAGLGEKKFRAGQLYQWMHEKLADSFDECTNLSNALRQKLKETSEYVCLEPVRVQHSKLDGTEKY
LFRLSDGNYVESVLMKYHHGNSVCISSQVGCRMGCRFCASTLNGKVRDLRPSEMLDQIYRIQKITGERVSNVVVMGSGEP
MDNYDNLIKFIELLNDERGLNISQRNITVSSCGIVPKLKELADLKLQITLAISLHAPNDELRKTMMPIANKYSIEEIMDV
CRYYIECTGRRISFEYSLVKGVNDSMECAKQLIELVKGMNCHINLIPVNPIKERDYKQTGKEEVYAFKNKLEKNGINVTI
RREMGRDIDGACGQLRNKYMEDIDESDGGY
>Mature_349_residues
TDIKSLNYDELVTYMAGLGEKKFRAGQLYQWMHEKLADSFDECTNLSNALRQKLKETSEYVCLEPVRVQHSKLDGTEKYL
FRLSDGNYVESVLMKYHHGNSVCISSQVGCRMGCRFCASTLNGKVRDLRPSEMLDQIYRIQKITGERVSNVVVMGSGEPM
DNYDNLIKFIELLNDERGLNISQRNITVSSCGIVPKLKELADLKLQITLAISLHAPNDELRKTMMPIANKYSIEEIMDVC
RYYIECTGRRISFEYSLVKGVNDSMECAKQLIELVKGMNCHINLIPVNPIKERDYKQTGKEEVYAFKNKLEKNGINVTIR
REMGRDIDGACGQLRNKYMEDIDESDGGY

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=339, Percent_Identity=40.7079646017699, Blast_Score=258, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_EUBE2 (C4Z523)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930174.1
- ProteinModelPortal:   C4Z523
- GeneID:   7957264
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00719
- OMA:   TFCSTAQ
- ProtClustDB:   CLSK2506256
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 40048; Mature: 39916

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIKSLNYDELVTYMAGLGEKKFRAGQLYQWMHEKLADSFDECTNLSNALRQKLKETSE
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YVCLEPVRVQHSKLDGTEKYLFRLSDGNYVESVLMKYHHGNSVCISSQVGCRMGCRFCAS
EEECCCHHHHHHHCCCCHHEEEEECCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHH
TLNGKVRDLRPSEMLDQIYRIQKITGERVSNVVVMGSGEPMDNYDNLIKFIELLNDERGL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHCCCCCC
NISQRNITVSSCGIVPKLKELADLKLQITLAISLHAPNDELRKTMMPIANKYSIEEIMDV
CEECCEEEEHHCCCCHHHHHHHCCEEEEEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHH
CRYYIECTGRRISFEYSLVKGVNDSMECAKQLIELVKGMNCHINLIPVNPIKERDYKQTG
HHHHHHHCCCEEEEEEHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHCC
KEEVYAFKNKLEKNGINVTIRREMGRDIDGACGQLRNKYMEDIDESDGGY
HHHHHHHHHHHHHCCCCEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDIKSLNYDELVTYMAGLGEKKFRAGQLYQWMHEKLADSFDECTNLSNALRQKLKETSE
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YVCLEPVRVQHSKLDGTEKYLFRLSDGNYVESVLMKYHHGNSVCISSQVGCRMGCRFCAS
EEECCCHHHHHHHCCCCHHEEEEECCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHH
TLNGKVRDLRPSEMLDQIYRIQKITGERVSNVVVMGSGEPMDNYDNLIKFIELLNDERGL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHCCCCCC
NISQRNITVSSCGIVPKLKELADLKLQITLAISLHAPNDELRKTMMPIANKYSIEEIMDV
CEECCEEEEHHCCCCHHHHHHHCCEEEEEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHH
CRYYIECTGRRISFEYSLVKGVNDSMECAKQLIELVKGMNCHINLIPVNPIKERDYKQTG
HHHHHHHCCCEEEEEEHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHCC
KEEVYAFKNKLEKNGINVTIRREMGRDIDGACGQLRNKYMEDIDESDGGY
HHHHHHHHHHHHHCCCCEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA