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The map label for this gene is def [H]

Identifier: 238916653

GI number: 238916653

Start: 718723

End: 719238

Strand: Direct

Name: def [H]

Synonym: EUBELI_00715

Alternate gene names: 238916653

Gene position: 718723-719238 (Clockwise)

Preceding gene: 238916652

Following gene: 238916654

Centisome position: 33.52

GC content: 37.98

Gene sequence:

>516_bases
ATGTACAATACGATATTAATGGATGATTGCGAGGAGGAATATATGGCAATAAGAAATATTAGAACACTTGGAGATGATAT
ACTAAGAGCCAAGGCAAAAGAGATTACAGAGATGACACCAAGAATTGAAGAGCTTATTGATGATATGTTTGATACAATGT
ATGAGGCTAATGGTGTAGGTCTTGCAGCACCTCAGGTTGGAATCAGAAAGAGACTTGTTGTTATTGACTGTGGAGATGAT
CCTATTGTTCTTATCAATCCTGTTGTATTAGAGACAAGCGGAAGTCAGACAGGGCTTGAAGGATGCCTAAGTGTACCTGG
AAAGACAGGTGAGGTTACAAGACCTAATTATGCCAAGGTTAAGGCTCTTAATGAGAATATGGAAGAGATTATAGTAGAAG
GAGAGGAGCTTCTTGCAAGATGCCTTCTTCATGAGATTGACCATCTTGATGGTATAATGTATGTTGATAAAGTAGAAGGA
GAACTTCTCTCTACAGATGACATTGAAGAAGAATAA

Upstream 100 bases:

>100_bases
AAGATAATCTATGTAAAACATAAAGACAGGGAAGTTCTTAACAGTATATATGAAAAGCTTGATGTTAATATTGTTGGAAA
TCAGGCTTTTAAGAATATTA

Downstream 100 bases:

>100_bases
TTAATGAAAAGGGGAACAGAATGCGAGTAGTATTTATGGGTACACCCGATTTTGCTGTAGGGACACTTAAAGCAATAATC
GAAGCAGGACATGATGTGGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MYNTILMDDCEEEYMAIRNIRTLGDDILRAKAKEITEMTPRIEELIDDMFDTMYEANGVGLAAPQVGIRKRLVVIDCGDD
PIVLINPVVLETSGSQTGLEGCLSVPGKTGEVTRPNYAKVKALNENMEEIIVEGEELLARCLLHEIDHLDGIMYVDKVEG
ELLSTDDIEEE

Sequences:

>Translated_171_residues
MYNTILMDDCEEEYMAIRNIRTLGDDILRAKAKEITEMTPRIEELIDDMFDTMYEANGVGLAAPQVGIRKRLVVIDCGDD
PIVLINPVVLETSGSQTGLEGCLSVPGKTGEVTRPNYAKVKALNENMEEIIVEGEELLARCLLHEIDHLDGIMYVDKVEG
ELLSTDDIEEE
>Mature_171_residues
MYNTILMDDCEEEYMAIRNIRTLGDDILRAKAKEITEMTPRIEELIDDMFDTMYEANGVGLAAPQVGIRKRLVVIDCGDD
PIVLINPVVLETSGSQTGLEGCLSVPGKTGEVTRPNYAKVKALNENMEEIIVEGEELLARCLLHEIDHLDGIMYVDKVEG
ELLSTDDIEEE

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=146, Percent_Identity=42.4657534246575, Blast_Score=114, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=31.3253012048193, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19075; Mature: 19075

Theoretical pI: Translated: 3.94; Mature: 3.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNTILMDDCEEEYMAIRNIRTLGDDILRAKAKEITEMTPRIEELIDDMFDTMYEANGVG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCC
LAAPQVGIRKRLVVIDCGDDPIVLINPVVLETSGSQTGLEGCLSVPGKTGEVTRPNYAKV
CCCCCCCCCEEEEEEECCCCCEEEECCEEEECCCCCCCHHHHHCCCCCCCCCCCCCHHHH
KALNENMEEIIVEGEELLARCLLHEIDHLDGIMYVDKVEGELLSTDDIEEE
HHHHCCHHHHHHCHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCC
>Mature Secondary Structure
MYNTILMDDCEEEYMAIRNIRTLGDDILRAKAKEITEMTPRIEELIDDMFDTMYEANGVG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCC
LAAPQVGIRKRLVVIDCGDDPIVLINPVVLETSGSQTGLEGCLSVPGKTGEVTRPNYAKV
CCCCCCCCCEEEEEEECCCCCEEEECCEEEECCCCCCCHHHHHCCCCCCCCCCCCCHHHH
KALNENMEEIIVEGEELLARCLLHEIDHLDGIMYVDKVEGELLSTDDIEEE
HHHHCCHHHHHHCHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA