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The map label for this gene is fmt

Identifier: 238916654

GI number: 238916654

Start: 719259

End: 720206

Strand: Direct

Name: fmt

Synonym: EUBELI_00716

Alternate gene names: 238916654

Gene position: 719259-720206 (Clockwise)

Preceding gene: 238916653

Following gene: 238916655

Centisome position: 33.54

GC content: 40.93

Gene sequence:

>948_bases
ATGCGAGTAGTATTTATGGGTACACCCGATTTTGCTGTAGGGACACTTAAAGCAATAATCGAAGCAGGACATGATGTGGC
AGCAGTTGTTACACAGCCTGATAAGCCAAGGGGAAGAAGCAAATCACTTGTTTTCTCTCCAGTTAAGGATGAAGCTGTGG
CACATGGAATCACAGTGTTACAGCCGGAGAGAGCAAGAGATGAAGCATTTGTAGAAGAATTAAGAACATATAATGCAGAT
GTAATTGTTGTCGTTGCATTTGGACAGTTACTGCCGGCAAGTATTATTAACATGCCAAGATACGGCTGTATCAATGTTCA
TGCATCACTTCTTCCTAAGTACAGAGGTGCATCGCCAATCCAGTGGGCAGTTATTGATGGCTGTGAGTATTCAGGTGTTA
CAACCATGAAGATGGATGAAGGCCTTGATACAGGAGATATCCTGATGGTTGAGAAGGTGAAGCTTGATGCTAAAGAGACG
GGCGGAAGTCTTTTTGACAGATTAAGTGATGTCGGTGCGCATCTTTTGGTTAAGACACTTGAAGGACTTGAAGCTGGAAC
TATTACTCCTGTTAAGCAGGATGATTCAGAAAGCACTTATGTAAAAATGCTTCATAAGTCATTTGGTAAGATGGATTTCA
ATAAGAGTGCAGCTGAACTTGAAAGACTTATAAGAGGACTTAATCCATGGCCTAGTGCATTTACTTATATAGACGGTAAG
ATGTTAAAGATATGGGATGCAGATGTTGCAGATAATATCAGTGAAGTACAGACAGAAGAAGTTAAGCCGGGACAGGTTGT
TACTGTAGGAAAGAACACATTTACAATAGCCTGTGGACAAGGTTACTTAGTTGTTAATGAGGTTCAGTTAGAAGGTAAGA
AGAGAATGGACAGCGGTTCATTCTTAAGAGGCAACCAGTTAGAAGCAGGTGTAATGTTAGGAGAATAA

Upstream 100 bases:

>100_bases
TTGACCATCTTGATGGTATAATGTATGTTGATAAAGTAGAAGGAGAACTTCTCTCTACAGATGACATTGAAGAAGAATAA
TTAATGAAAAGGGGAACAGA

Downstream 100 bases:

>100_bases
GCAGAATCTTAAATGGTTCAGAAAAGAGGTATTATGGTAGCTTTATATGGTTATTATGGATATGGATATTACTATGATCC
AATGTACATTCTGATAATAA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRVVFMGTPDFAVGTLKAIIEAGHDVAAVVTQPDKPRGRSKSLVFSPVKDEAVAHGITVLQPERARDEAFVEELRTYNAD
VIVVVAFGQLLPASIINMPRYGCINVHASLLPKYRGASPIQWAVIDGCEYSGVTTMKMDEGLDTGDILMVEKVKLDAKET
GGSLFDRLSDVGAHLLVKTLEGLEAGTITPVKQDDSESTYVKMLHKSFGKMDFNKSAAELERLIRGLNPWPSAFTYIDGK
MLKIWDADVADNISEVQTEEVKPGQVVTVGKNTFTIACGQGYLVVNEVQLEGKKRMDSGSFLRGNQLEAGVMLGE

Sequences:

>Translated_315_residues
MRVVFMGTPDFAVGTLKAIIEAGHDVAAVVTQPDKPRGRSKSLVFSPVKDEAVAHGITVLQPERARDEAFVEELRTYNAD
VIVVVAFGQLLPASIINMPRYGCINVHASLLPKYRGASPIQWAVIDGCEYSGVTTMKMDEGLDTGDILMVEKVKLDAKET
GGSLFDRLSDVGAHLLVKTLEGLEAGTITPVKQDDSESTYVKMLHKSFGKMDFNKSAAELERLIRGLNPWPSAFTYIDGK
MLKIWDADVADNISEVQTEEVKPGQVVTVGKNTFTIACGQGYLVVNEVQLEGKKRMDSGSFLRGNQLEAGVMLGE
>Mature_315_residues
MRVVFMGTPDFAVGTLKAIIEAGHDVAAVVTQPDKPRGRSKSLVFSPVKDEAVAHGITVLQPERARDEAFVEELRTYNAD
VIVVVAFGQLLPASIINMPRYGCINVHASLLPKYRGASPIQWAVIDGCEYSGVTTMKMDEGLDTGDILMVEKVKLDAKET
GGSLFDRLSDVGAHLLVKTLEGLEAGTITPVKQDDSESTYVKMLHKSFGKMDFNKSAAELERLIRGLNPWPSAFTYIDGK
MLKIWDADVADNISEVQTEEVKPGQVVTVGKNTFTIACGQGYLVVNEVQLEGKKRMDSGSFLRGNQLEAGVMLGE

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=318, Percent_Identity=27.6729559748428, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI238814322, Length=309, Percent_Identity=26.537216828479, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI164663775, Length=321, Percent_Identity=28.3489096573209, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1789683, Length=306, Percent_Identity=42.156862745098, Blast_Score=257, Evalue=8e-70,
Organism=Escherichia coli, GI1788589, Length=316, Percent_Identity=25.9493670886076, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI133930964, Length=336, Percent_Identity=26.7857142857143, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45550868, Length=307, Percent_Identity=30.6188925081433, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28571984, Length=229, Percent_Identity=34.061135371179, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24585660, Length=245, Percent_Identity=25.7142857142857, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_EUBE2 (C4Z520)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930171.1
- GeneID:   7957261
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00716
- OMA:   IMQMDEG
- ProtClustDB:   CLSK2548277
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34295; Mature: 34295

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVFMGTPDFAVGTLKAIIEAGHDVAAVVTQPDKPRGRSKSLVFSPVKDEAVAHGITVL
CEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEECCCCHHHHHCCEEEE
QPERARDEAFVEELRTYNADVIVVVAFGQLLPASIINMPRYGCINVHASLLPKYRGASPI
CCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCCCCE
QWAVIDGCEYSGVTTMKMDEGLDTGDILMVEKVKLDAKETGGSLFDRLSDVGAHLLVKTL
EEEEECCCCCCCEEEEEECCCCCCCCEEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHH
EGLEAGTITPVKQDDSESTYVKMLHKSFGKMDFNKSAAELERLIRGLNPWPSAFTYIDGK
HCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCE
MLKIWDADVADNISEVQTEEVKPGQVVTVGKNTFTIACGQGYLVVNEVQLEGKKRMDSGS
EEEEECCHHHCCHHHHHHHCCCCCEEEEECCCEEEEEECCCEEEEEEEEECCCCCCCCCC
FLRGNQLEAGVMLGE
CEECCCCCCCEEECC
>Mature Secondary Structure
MRVVFMGTPDFAVGTLKAIIEAGHDVAAVVTQPDKPRGRSKSLVFSPVKDEAVAHGITVL
CEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEECCCCHHHHHCCEEEE
QPERARDEAFVEELRTYNADVIVVVAFGQLLPASIINMPRYGCINVHASLLPKYRGASPI
CCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCCCCE
QWAVIDGCEYSGVTTMKMDEGLDTGDILMVEKVKLDAKETGGSLFDRLSDVGAHLLVKTL
EEEEECCCCCCCEEEEEECCCCCCCCEEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHH
EGLEAGTITPVKQDDSESTYVKMLHKSFGKMDFNKSAAELERLIRGLNPWPSAFTYIDGK
HCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCE
MLKIWDADVADNISEVQTEEVKPGQVVTVGKNTFTIACGQGYLVVNEVQLEGKKRMDSGS
EEEEECCHHHCCHHHHHHHCCCCCEEEEECCCEEEEEECCCEEEEEEEEECCCCCCCCCC
FLRGNQLEAGVMLGE
CEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA