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The map label for this gene is leuS [H]

Identifier: 238916624

GI number: 238916624

Start: 681257

End: 683707

Strand: Direct

Name: leuS [H]

Synonym: EUBELI_00681

Alternate gene names: 238916624

Gene position: 681257-683707 (Clockwise)

Preceding gene: 238916622

Following gene: 238916628

Centisome position: 31.77

GC content: 40.07

Gene sequence:

>2451_bases
ATGTTTTTATGCGTTTTTAAGGAGGAATGTGTTATGTCGGCAGTATATAACCACAAAACAGTTGAAAAGAAGTGGCAGCA
GATATGGCAGGATGAAAAAGCCTTTGCTGCAACTAATGATTATTCAAAACCAAAGTATTATGCACTTGTGGAGTTCCCAT
ATCCATCAGGACAGGGGCTTCATGTAGGTCATCCAAGACCATATACAGCGCTTGATATCGTAGCAAGAAAGAGAAGAATG
CAGGGATATAATGTTTTATATCCAATGGGTTGGGATGCATTTGGTCTTCCAACAGAGAACTATGCTATTAAGAACAAGAT
TCACCCTAAGATAGTTACTAAGAATAATATAGATCATTTTAAGGAACAGCTTCAGTCAATTGGTTATTCATTTGACTGGG
ACAGAGAGATTAATACTACAGATCCTGATTATTATAAGTGGACTCAGTGGATTTTCCTTAAGCTTTTTAAGGCTGGACTT
GCATATAAGCAGGAGATGCCTATCAACTGGTGTACTTCATGTAAGGTAGGTCTTGCTAATGAAGAAGTAGTTAATGGTGT
ATGTGAAAGATGTGGTTCACCTGTTGTCCGTAAGGTCAAGAGTGAGTGGATGCTTAAGATTACTGATTATGCAGATAAGC
TTATAGAAGGACTTGACCATGTCGATTATATCGAGAGAGTTAAGACACAGCAGAAGAACTGGATTGGACGTTCTACAGGT
GCTGAGGTTGATTTTGCAATTGCTGGTAAGGAAGATAAGTTAAGAATATATACAACAAGATGCGATACACTTTTCGGTGT
TACTTATATGGTTGTATCACCAGAGCATCCATATCTTGATAAGTATAAGGATGAGATTAAGAACTGGGATGAAATAGAAA
GCTATAGAGAGCAGGCTTCAAGAAAGTCAGATTTCGAGAGAGCAGAGCTTGCAAAGGATAAGACAGGTGTAGAGATTGAC
GGACTTAAGGCAGTTAATCCGGTTAATGGCAAGGAAGTTCCAATCTGGGTATCAGATTATGTACTTATGAGCTATGGTAC
AGGTGCTATCATGGCTGTTCCTGCTCACGATGAAAGAGACTGGGAGTTTGCCAAGAAATTTAACCTTCCTATCATTCAGG
TTGTAGCAAAGAACGGTGAAGAAGTTGATGTTAATGAGGCAGCATTTACAGATGTAGCAACAGGTGTTCTTATTAATTCG
GATTTCATTAACGGACTTGAAGTTAAAGAAGCTAAGGAAAAGATGATTGAATTCCTTACAGAAAAGGGAATTGGTAATGC
AAAGGTTAATTATAAGCTCCGTGACTGGGTATTCTCTCGTCAGAGATACTGGGGAGAGCCTATTCCAATTGTTCATTGTG
ACAAATGTGGCTATGTTCCAGTTGATGAGAGCGAACTTCCATTACTTCTTCCAGAGGTAGAAAGTTATATGCCTACAGAT
AATGGAGAATCACCTCTTGCAGCCATGACAGACTGGGTTAATACAACATGCCCATGCTGTGGCGGACCAGCTAAGAGAGA
GACTGATACAATGCCTCAGTGGGCTGGATCATCATGGTATTTCTTAAGATATACAGATCCACATAACACAGAGACACTCG
CAAGTCAGGAAGCACTTAAGTACTGGCTTCCGGTAGACTGGTATAATGGAGGAATGGAGCATACAACATTACACTTACTC
TATTCAAGATTCTGGCACAGATTCCTTTACGACCAGAAGGTTGTACCTTGCCCAGAGCCATATCAGAAGAGAACATCCCA
CGGTATGATTCTAGGTGAGAACGGCGAGAAGATGAGTAAGTCAAGAGGCAATGTTGTTAATCCTGATGATATCGTAAGAG
ATTATGGTGCAGATACATTAAGAACATACGAGATGTTTATCGGTGCATTTGACGCAGCAGCTTCATGGTCAGAGGATGGC
GTTAAGGGCTGCCGCAGATTCTTAGACAGAGTATGGAAGCTTCAGGATATAATGACTGATGAGGAAGGTTTCTCTAAGGA
ATTTGAGACAAAGATGCATCAGACAATTAAGAAAGTTTCATTTGATTATGAGAATCTGAAATACAATACAGCTATTGCAC
AGCTCATGACTATGCTCAATGATTTCAGCAAGGCAGGTAAGATTACTAAGGGAGAACTCAAGACATACCTTATCTTACTT
AATCCGGTTGCTCCACACATTACAGAAGAGATGTGGGAAGCAATCGGCGAGAACGGAAGAGTATATCAGCAGACATGGCC
TGAATACGATGAAGCTAAGACAGTTGAGTCAATGGTTGAGATAGCAGTCCAGATTAATGGAAAGACTAAGGCTACTATCA
ATATTGCAAAGGATGCTGACAAGGACAGTGTAATTGCTGCAGCTAAGGAAGCACTTGGAAGCAGGCTTTCTGGTAACATT
ATTAAAGAAATATATGTACCGGGAAGAATTGTTAATATTGTTGCAAAGTAA

Upstream 100 bases:

>100_bases
AAGAAATATTAATATTCATGTGAATAGTGGTATTTTAACACTTTAAAGCGATAATGTAAAGGCGGCTATTTAATTGACAA
ATTAAAGTGATGTGTGTAGA

Downstream 100 bases:

>100_bases
TTAAGAATAATAAAATATTAATTCATATTAAGAAGAGAGAGAAGCTCAGGCAGAAGCCTGAGCTCTTTTTCGCTTAGCTG
CCCAGACATACTGTTGATGA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 816; Mature: 816

Protein sequence:

>816_residues
MFLCVFKEECVMSAVYNHKTVEKKWQQIWQDEKAFAATNDYSKPKYYALVEFPYPSGQGLHVGHPRPYTALDIVARKRRM
QGYNVLYPMGWDAFGLPTENYAIKNKIHPKIVTKNNIDHFKEQLQSIGYSFDWDREINTTDPDYYKWTQWIFLKLFKAGL
AYKQEMPINWCTSCKVGLANEEVVNGVCERCGSPVVRKVKSEWMLKITDYADKLIEGLDHVDYIERVKTQQKNWIGRSTG
AEVDFAIAGKEDKLRIYTTRCDTLFGVTYMVVSPEHPYLDKYKDEIKNWDEIESYREQASRKSDFERAELAKDKTGVEID
GLKAVNPVNGKEVPIWVSDYVLMSYGTGAIMAVPAHDERDWEFAKKFNLPIIQVVAKNGEEVDVNEAAFTDVATGVLINS
DFINGLEVKEAKEKMIEFLTEKGIGNAKVNYKLRDWVFSRQRYWGEPIPIVHCDKCGYVPVDESELPLLLPEVESYMPTD
NGESPLAAMTDWVNTTCPCCGGPAKRETDTMPQWAGSSWYFLRYTDPHNTETLASQEALKYWLPVDWYNGGMEHTTLHLL
YSRFWHRFLYDQKVVPCPEPYQKRTSHGMILGENGEKMSKSRGNVVNPDDIVRDYGADTLRTYEMFIGAFDAAASWSEDG
VKGCRRFLDRVWKLQDIMTDEEGFSKEFETKMHQTIKKVSFDYENLKYNTAIAQLMTMLNDFSKAGKITKGELKTYLILL
NPVAPHITEEMWEAIGENGRVYQQTWPEYDEAKTVESMVEIAVQINGKTKATINIAKDADKDSVIAAAKEALGSRLSGNI
IKEIYVPGRIVNIVAK

Sequences:

>Translated_816_residues
MFLCVFKEECVMSAVYNHKTVEKKWQQIWQDEKAFAATNDYSKPKYYALVEFPYPSGQGLHVGHPRPYTALDIVARKRRM
QGYNVLYPMGWDAFGLPTENYAIKNKIHPKIVTKNNIDHFKEQLQSIGYSFDWDREINTTDPDYYKWTQWIFLKLFKAGL
AYKQEMPINWCTSCKVGLANEEVVNGVCERCGSPVVRKVKSEWMLKITDYADKLIEGLDHVDYIERVKTQQKNWIGRSTG
AEVDFAIAGKEDKLRIYTTRCDTLFGVTYMVVSPEHPYLDKYKDEIKNWDEIESYREQASRKSDFERAELAKDKTGVEID
GLKAVNPVNGKEVPIWVSDYVLMSYGTGAIMAVPAHDERDWEFAKKFNLPIIQVVAKNGEEVDVNEAAFTDVATGVLINS
DFINGLEVKEAKEKMIEFLTEKGIGNAKVNYKLRDWVFSRQRYWGEPIPIVHCDKCGYVPVDESELPLLLPEVESYMPTD
NGESPLAAMTDWVNTTCPCCGGPAKRETDTMPQWAGSSWYFLRYTDPHNTETLASQEALKYWLPVDWYNGGMEHTTLHLL
YSRFWHRFLYDQKVVPCPEPYQKRTSHGMILGENGEKMSKSRGNVVNPDDIVRDYGADTLRTYEMFIGAFDAAASWSEDG
VKGCRRFLDRVWKLQDIMTDEEGFSKEFETKMHQTIKKVSFDYENLKYNTAIAQLMTMLNDFSKAGKITKGELKTYLILL
NPVAPHITEEMWEAIGENGRVYQQTWPEYDEAKTVESMVEIAVQINGKTKATINIAKDADKDSVIAAAKEALGSRLSGNI
IKEIYVPGRIVNIVAK
>Mature_816_residues
MFLCVFKEECVMSAVYNHKTVEKKWQQIWQDEKAFAATNDYSKPKYYALVEFPYPSGQGLHVGHPRPYTALDIVARKRRM
QGYNVLYPMGWDAFGLPTENYAIKNKIHPKIVTKNNIDHFKEQLQSIGYSFDWDREINTTDPDYYKWTQWIFLKLFKAGL
AYKQEMPINWCTSCKVGLANEEVVNGVCERCGSPVVRKVKSEWMLKITDYADKLIEGLDHVDYIERVKTQQKNWIGRSTG
AEVDFAIAGKEDKLRIYTTRCDTLFGVTYMVVSPEHPYLDKYKDEIKNWDEIESYREQASRKSDFERAELAKDKTGVEID
GLKAVNPVNGKEVPIWVSDYVLMSYGTGAIMAVPAHDERDWEFAKKFNLPIIQVVAKNGEEVDVNEAAFTDVATGVLINS
DFINGLEVKEAKEKMIEFLTEKGIGNAKVNYKLRDWVFSRQRYWGEPIPIVHCDKCGYVPVDESELPLLLPEVESYMPTD
NGESPLAAMTDWVNTTCPCCGGPAKRETDTMPQWAGSSWYFLRYTDPHNTETLASQEALKYWLPVDWYNGGMEHTTLHLL
YSRFWHRFLYDQKVVPCPEPYQKRTSHGMILGENGEKMSKSRGNVVNPDDIVRDYGADTLRTYEMFIGAFDAAASWSEDG
VKGCRRFLDRVWKLQDIMTDEEGFSKEFETKMHQTIKKVSFDYENLKYNTAIAQLMTMLNDFSKAGKITKGELKTYLILL
NPVAPHITEEMWEAIGENGRVYQQTWPEYDEAKTVESMVEIAVQINGKTKATINIAKDADKDSVIAAAKEALGSRLSGNI
IKEIYVPGRIVNIVAK

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=880, Percent_Identity=33.4090909090909, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI268370297, Length=459, Percent_Identity=22.6579520697168, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI268370293, Length=459, Percent_Identity=22.6579520697168, Blast_Score=97, Evalue=5e-20,
Organism=Escherichia coli, GI1786861, Length=874, Percent_Identity=40.2745995423341, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1790708, Length=397, Percent_Identity=24.9370277078086, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71997510, Length=275, Percent_Identity=37.0909090909091, Blast_Score=172, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI71997517, Length=271, Percent_Identity=36.90036900369, Blast_Score=169, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI212645227, Length=309, Percent_Identity=28.4789644012945, Blast_Score=125, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17510661, Length=696, Percent_Identity=21.4080459770115, Blast_Score=118, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17554638, Length=289, Percent_Identity=23.5294117647059, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323414, Length=854, Percent_Identity=37.7049180327869, Blast_Score=505, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6321531, Length=434, Percent_Identity=24.1935483870968, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6325217, Length=145, Percent_Identity=30.3448275862069, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21355409, Length=815, Percent_Identity=31.6564417177914, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI17864482, Length=461, Percent_Identity=22.7765726681128, Blast_Score=109, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24653289, Length=461, Percent_Identity=22.7765726681128, Blast_Score=109, Evalue=7e-24,
Organism=Drosophila melanogaster, GI21355675, Length=692, Percent_Identity=21.6763005780347, Blast_Score=102, Evalue=2e-21,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 93576; Mature: 93576

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLCVFKEECVMSAVYNHKTVEKKWQQIWQDEKAFAATNDYSKPKYYALVEFPYPSGQGL
CEEEEEHHHHHHHHHHCCHHHHHHHHHHHCCCHHEECCCCCCCCCEEEEEEECCCCCCCE
HVGHPRPYTALDIVARKRRMQGYNVLYPMGWDAFGLPTENYAIKNKIHPKIVTKNNIDHF
ECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEECCCCCCEEEECCCHHHH
KEQLQSIGYSFDWDREINTTDPDYYKWTQWIFLKLFKAGLAYKQEMPINWCTSCKVGLAN
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCC
EEVVNGVCERCGSPVVRKVKSEWMLKITDYADKLIEGLDHVDYIERVKTQQKNWIGRSTG
HHHHHHHHHHCCCHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AEVDFAIAGKEDKLRIYTTRCDTLFGVTYMVVSPEHPYLDKYKDEIKNWDEIESYREQAS
CCEEEEEECCCCCEEEEEEECCHHHCEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHH
RKSDFERAELAKDKTGVEIDGLKAVNPVNGKEVPIWVSDYVLMSYGTGAIMAVPAHDERD
HHCCHHHHHHHCCCCCCEECCCEEECCCCCCCCEEEEECEEEEEECCCEEEEECCCCCCC
WEFAKKFNLPIIQVVAKNGEEVDVNEAAFTDVATGVLINSDFINGLEVKEAKEKMIEFLT
HHHHHHCCCCEEEEEECCCCEECCCHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHH
EKGIGNAKVNYKLRDWVFSRQRYWGEPIPIVHCDKCGYVPVDESELPLLLPEVESYMPTD
HCCCCCCEEEEEHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEECCCHHHHCCCC
NGESPLAAMTDWVNTTCPCCGGPAKRETDTMPQWAGSSWYFLRYTDPHNTETLASQEALK
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
YWLPVDWYNGGMEHTTLHLLYSRFWHRFLYDQKVVPCPEPYQKRTSHGMILGENGEKMSK
HCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEECCCCCHHHH
SRGNVVNPDDIVRDYGADTLRTYEMFIGAFDAAASWSEDGVKGCRRFLDRVWKLQDIMTD
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
EEGFSKEFETKMHQTIKKVSFDYENLKYNTAIAQLMTMLNDFSKAGKITKGELKTYLILL
CCCCHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
NPVAPHITEEMWEAIGENGRVYQQTWPEYDEAKTVESMVEIAVQINGKTKATINIAKDAD
CCCCHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHEEECCCCEEEEEEECCCC
KDSVIAAAKEALGSRLSGNIIKEIYVPGRIVNIVAK
CHHHHHHHHHHHHCHHCCCHHHHHCCCCEEEEEECC
>Mature Secondary Structure
MFLCVFKEECVMSAVYNHKTVEKKWQQIWQDEKAFAATNDYSKPKYYALVEFPYPSGQGL
CEEEEEHHHHHHHHHHCCHHHHHHHHHHHCCCHHEECCCCCCCCCEEEEEEECCCCCCCE
HVGHPRPYTALDIVARKRRMQGYNVLYPMGWDAFGLPTENYAIKNKIHPKIVTKNNIDHF
ECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEECCCCCCEEEECCCHHHH
KEQLQSIGYSFDWDREINTTDPDYYKWTQWIFLKLFKAGLAYKQEMPINWCTSCKVGLAN
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCC
EEVVNGVCERCGSPVVRKVKSEWMLKITDYADKLIEGLDHVDYIERVKTQQKNWIGRSTG
HHHHHHHHHHCCCHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AEVDFAIAGKEDKLRIYTTRCDTLFGVTYMVVSPEHPYLDKYKDEIKNWDEIESYREQAS
CCEEEEEECCCCCEEEEEEECCHHHCEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHH
RKSDFERAELAKDKTGVEIDGLKAVNPVNGKEVPIWVSDYVLMSYGTGAIMAVPAHDERD
HHCCHHHHHHHCCCCCCEECCCEEECCCCCCCCEEEEECEEEEEECCCEEEEECCCCCCC
WEFAKKFNLPIIQVVAKNGEEVDVNEAAFTDVATGVLINSDFINGLEVKEAKEKMIEFLT
HHHHHHCCCCEEEEEECCCCEECCCHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHH
EKGIGNAKVNYKLRDWVFSRQRYWGEPIPIVHCDKCGYVPVDESELPLLLPEVESYMPTD
HCCCCCCEEEEEHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEECCCHHHHCCCC
NGESPLAAMTDWVNTTCPCCGGPAKRETDTMPQWAGSSWYFLRYTDPHNTETLASQEALK
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
YWLPVDWYNGGMEHTTLHLLYSRFWHRFLYDQKVVPCPEPYQKRTSHGMILGENGEKMSK
HCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEECCCCCHHHH
SRGNVVNPDDIVRDYGADTLRTYEMFIGAFDAAASWSEDGVKGCRRFLDRVWKLQDIMTD
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
EEGFSKEFETKMHQTIKKVSFDYENLKYNTAIAQLMTMLNDFSKAGKITKGELKTYLILL
CCCCHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
NPVAPHITEEMWEAIGENGRVYQQTWPEYDEAKTVESMVEIAVQINGKTKATINIAKDAD
CCCCHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHEEECCCCEEEEEEECCCC
KDSVIAAAKEALGSRLSGNIIKEIYVPGRIVNIVAK
CHHHHHHHHHHHHCHHCCCHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA