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The map label for this gene is ynfL [H]

Identifier: 238916603

GI number: 238916603

Start: 660840

End: 661739

Strand: Direct

Name: ynfL [H]

Synonym: EUBELI_00660

Alternate gene names: 238916603

Gene position: 660840-661739 (Clockwise)

Preceding gene: 238916601

Following gene: 238916604

Centisome position: 30.82

GC content: 37.0

Gene sequence:

>900_bases
ATGACATTACAGCAGTTAAGATACATCGTTATGGTTGCAGAGACAGGCACGATTACTGAGGCGGCAGGGAAATTATATAT
TTCACAGCCAAGCCTTACCAATGCAATTCATGAACTTGAAAAAGAGATGAATATTACGATATTCAACAGAACTAATAAAG
GAATAAGTGTATCTAAAGAAGGTGAAGAGTTCTTATCGTATGCAAGACAGATTCTTGACCAGGCGGCAATCTTAGAGGAT
AAGTATAAGGGCGATAATGGTGGAAGAAAGCAATATTGTATATCAACACAGCATTATTCTTTTGCAGTCAACGCATTTGT
TGACCTTATAAAGCAGTTCGGGCAGGATGAGTATGATTTCAGCTTAAGAGAAACGCAGACTTATGAGATTATCGAGGATG
TTGCAAGGTTAAGAAGTGAGATTGGAATACTGTTCATTAATGATTTTAATGAGGCTGTTATTAGAAAGATTTTAAAGTCA
TATGAACTTGAATTTCATGAGCTTTTTACAGCAAGACCACATGTATTCATAAGCAGAAAACATCCACTTGCAGAGTGCAG
TGTCATTCATAATGAGCAGTTAGAAGAATATCCTTATCTGTCATTTGAGCAGGGCGAACATAATTCATTCTATTTCTCAG
AGGAGATATTCTCAGAAACAGCAAGAAAGAAGAACATAAGAGTCCGTGACAGGGCAACACTTTTTAATCTGCTGATTGGT
CTTAATGGTTACACAGTAAGTAGTGGTATTATTGACAAGAAGCTCAACGGAAAAGACATTATAGCAGTGCCGCTTGCTGA
TGAATCAGATATGCATATTGGTTACATAACTCACAGAAAAGGGATGCTTAGCAGGCTTGGAAGTACATATCTTGAGGCTA
TAAGGAAGTATCTTAAATAG

Upstream 100 bases:

>100_bases
ATAATCAGAAATTTTTACGGGCTGATTGTATCATAATATTTTTTAAAAAACAACAAAAATATGTTACAATATTTTAAATA
TTATTCAGGCAGGTAAGAGT

Downstream 100 bases:

>100_bases
TCATTGCATATATGTAAAATGAGTTAGCCATAGTTTTAAACTATGACTAACTCTTTTTTTTATGTATTATACAAATGCTT
TTTTGGAATTTATAATGACA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTLQQLRYIVMVAETGTITEAAGKLYISQPSLTNAIHELEKEMNITIFNRTNKGISVSKEGEEFLSYARQILDQAAILED
KYKGDNGGRKQYCISTQHYSFAVNAFVDLIKQFGQDEYDFSLRETQTYEIIEDVARLRSEIGILFINDFNEAVIRKILKS
YELEFHELFTARPHVFISRKHPLAECSVIHNEQLEEYPYLSFEQGEHNSFYFSEEIFSETARKKNIRVRDRATLFNLLIG
LNGYTVSSGIIDKKLNGKDIIAVPLADESDMHIGYITHRKGMLSRLGSTYLEAIRKYLK

Sequences:

>Translated_299_residues
MTLQQLRYIVMVAETGTITEAAGKLYISQPSLTNAIHELEKEMNITIFNRTNKGISVSKEGEEFLSYARQILDQAAILED
KYKGDNGGRKQYCISTQHYSFAVNAFVDLIKQFGQDEYDFSLRETQTYEIIEDVARLRSEIGILFINDFNEAVIRKILKS
YELEFHELFTARPHVFISRKHPLAECSVIHNEQLEEYPYLSFEQGEHNSFYFSEEIFSETARKKNIRVRDRATLFNLLIG
LNGYTVSSGIIDKKLNGKDIIAVPLADESDMHIGYITHRKGMLSRLGSTYLEAIRKYLK
>Mature_298_residues
TLQQLRYIVMVAETGTITEAAGKLYISQPSLTNAIHELEKEMNITIFNRTNKGISVSKEGEEFLSYARQILDQAAILEDK
YKGDNGGRKQYCISTQHYSFAVNAFVDLIKQFGQDEYDFSLRETQTYEIIEDVARLRSEIGILFINDFNEAVIRKILKSY
ELEFHELFTARPHVFISRKHPLAECSVIHNEQLEEYPYLSFEQGEHNSFYFSEEIFSETARKKNIRVRDRATLFNLLIGL
NGYTVSSGIIDKKLNGKDIIAVPLADESDMHIGYITHRKGMLSRLGSTYLEAIRKYLK

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=215, Percent_Identity=28.8372093023256, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693105, Length=291, Percent_Identity=23.3676975945017, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788887, Length=289, Percent_Identity=26.2975778546713, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34481; Mature: 34349

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQQLRYIVMVAETGTITEAAGKLYISQPSLTNAIHELEKEMNITIFNRTNKGISVSKE
CCHHHEEEEEEEECCCCEEHHCCEEEEECCHHHHHHHHHHHHCCEEEEECCCCCCCCCCC
GEEFLSYARQILDQAAILEDKYKGDNGGRKQYCISTQHYSFAVNAFVDLIKQFGQDEYDF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCC
SLRETQTYEIIEDVARLRSEIGILFINDFNEAVIRKILKSYELEFHELFTARPHVFISRK
CHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCHHHHHHCCCCCEEEECC
HPLAECSVIHNEQLEEYPYLSFEQGEHNSFYFSEEIFSETARKKNIRVRDRATLFNLLIG
CCCHHHHHHHHHHHHHCCCCEEECCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LNGYTVSSGIIDKKLNGKDIIAVPLADESDMHIGYITHRKGMLSRLGSTYLEAIRKYLK
CCCEEECCCCEECCCCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLQQLRYIVMVAETGTITEAAGKLYISQPSLTNAIHELEKEMNITIFNRTNKGISVSKE
CHHHEEEEEEEECCCCEEHHCCEEEEECCHHHHHHHHHHHHCCEEEEECCCCCCCCCCC
GEEFLSYARQILDQAAILEDKYKGDNGGRKQYCISTQHYSFAVNAFVDLIKQFGQDEYDF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCC
SLRETQTYEIIEDVARLRSEIGILFINDFNEAVIRKILKSYELEFHELFTARPHVFISRK
CHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCHHHHHHCCCCCEEEECC
HPLAECSVIHNEQLEEYPYLSFEQGEHNSFYFSEEIFSETARKKNIRVRDRATLFNLLIG
CCCHHHHHHHHHHHHHCCCCEEECCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LNGYTVSSGIIDKKLNGKDIIAVPLADESDMHIGYITHRKGMLSRLGSTYLEAIRKYLK
CCCEEECCCCEECCCCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]