| Definition | Eubacterium eligens ATCC 27750 chromosome, complete genome. |
|---|---|
| Accession | NC_012778 |
| Length | 2,144,190 |
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The map label for this gene is metE [H]
Identifier: 238916604
GI number: 238916604
Start: 661854
End: 664139
Strand: Direct
Name: metE [H]
Synonym: EUBELI_00661
Alternate gene names: 238916604
Gene position: 661854-664139 (Clockwise)
Preceding gene: 238916603
Following gene: 238916605
Centisome position: 30.87
GC content: 38.1
Gene sequence:
>2286_bases ATGAGGAGAGCAGGTATGAAGACAGCAGTAGCAGGTTATCCAAGGATAGGAACATTAAGAGAGTTAAAGTTTGCATTAGA AAAATATTTCAGAAAAGAAATAAGTGCAGATGAACTGACACAGACAGCAAAGGAGTTACGAAAGACACACTGGCTTATAC AGAAAGAGGCAGGAATTGATTATATCACAAGCAATGATTTTTCATATTATGATATAGTGCTTGATACAGCATTTTTACTG AATATAATTTCTGAAAGATACAATGAACTTGAGGTTTCAGAGCTTGATAAATATCTTGCAATGGCAAGGGGCTATCAGGG AGAGAACGGTGATGTAAAAGCCCTTGCAATGAAGAAATGGTTTAATACTAATTACCACTATATTGTTCCAGAGGTTGAGG ACAGCACGCAGATAAGACTTACAGGTAATAAGCTGTGGGCAGAATATGCAGAGGCAAAAGAACTTGGAATTGAGACAAAA CCGGTAATCACAGGTGTGTATACATTATTTAAGCTGTGCAGATTTACAGGCAAGAAGAAGGCAGATGATTTTATCAATGC ATTTATTGAAGCATATAAAGATGTTTATAGCAAATGTGAAGCTGTAGGAATCCAGTGGCTTCAGTTTGATGAGCCTGCAC TTGTACAGGACATGACAGAAGAAGACAGAGAACTGTTTGTCAAAATGTATAGTGATATTTTAGGTAAGAAGAAATCATGC AAAGTATTGCTTCAGACATATTTCGGAGACGTGAGGGATGTGTATGAAGATATTGTTAAGCTGTCGTTTGACGGAATCGG ACTTGATTTTATTGAGGGAAAGAAGACAGCAGAACTCATTGAAAAATATGGATTTCCAAAGGACACGATATTATTCGCTG GACTTGTAAATGGAAAGAATATCTGGAAGAACCATTATGAGAAGACTTTGAATGTGCTTAAAAAGCTTGAAGATAAGGGA ATACAGACGGTTTTATCAACATCATGTTCGCTGCAGCATGTTCCATATACATTAAAGCATGAGAATAAACTTTCGGATGA ATATCTGAATTACTTCGCATTTGCAGAAGAAAAGCTTGTGGAATTAAAGGAATTAAGCGTGCTTGCCGAGTGTGGAAATA TTGAAGAAGATGAAAGATTCAAGACAAACAGGAAGCTTTTTGCAGGTACAAGAAAATGTGATAATGAATCTGTAAAGAAA AGACTTGCGGAAGTTACAGAGGCGGATTACAGAAGACTTCCTGCAAGAAGTGAGAGACAGCAGCTTCAGAAGAAAGAATT TGCATTACCGAAGCTGCCTACAACTACAATCGGTTCATTTCCACAGACAAAAGATGTTAAGGCCAACCGTTCAGCATTCC GTAAGGGTGAAATCAGCGAAGAACAGTATGTTGAATTTAATAAGAAGAAAATTGAAGAATGTGTCAGATGGCAGGAAAAA ATCGGACTTGATGTACTTGTTCATGGAGAATACGAAAGAAATGACATGGTTGAATATTTCGGTGAAGCGTTGGGAGGTTT TCTGTTCACAGAGAAAGCATGGGTTCAGTCATATGGAACAAGATGTGTTAAACCACCTGTTATATGGGGAGATGTTTACC GCAAGAAGCCGATAACAGTTGAGTGGTCTGTGTATGCACAGTCACTTACAGATAAGATAATGAAAGGAATGCTTACAGGT CCGGTAACAATTCTTAACTGGTCATTCCCAAGAGAAGATATTACAATCAAGGAATCAATTTCGCAGATTGCACTTGCAAT AAGAGATGAGGTGCTTGACCTTGAAGCAAATGGAATTAAGATTATTCAGATTGATGAGGCAGCTTTAAGAGAAAAGCTTC CATTAAGAAAATCTGACTGGAATACAGAATATCTTGATTTTTCAATACCAGCATTCAGACTTACTGCAAGCGGTGTAAAA CCTGAAACACAGATACATACTCATATGTGTTACAGTGAATTTACAGATATAATCCCAGCAATAGACGATATGGACGCTGA TGTTATCACATTTGAAGCATCACGCTCAGACCTTCAGATTCTTGATTCGTTAAGAGAAAACAACTTTGAAACAGAGGTTG GACCGGGAGTATACGACATTCATTCACCAAGAATACCGTCGGTAGAAGAGATTACCCGTGCAATAAAGATAATGCTAACA AAGATAGACAAAGACAAGTTGTGGGTAAATCCAGACTGTGGACTTAAGACAAGAGGTGTGCCAGAGACAGAAGCAAGCCT TAAGAATATGGTTAAGGCAGCTGAGATTATCAGGGCTGGGTTGTAG
Upstream 100 bases:
>100_bases TAAAATGAGTTAGCCATAGTTTTAAACTATGACTAACTCTTTTTTTTATGTATTATACAAATGCTTTTTTGGAATTTATA ATGACAATATCAAATAATAG
Downstream 100 bases:
>100_bases TGTAGGCATTTGTGAGACTGGAGATGGTTTATTGTGAGGCGGCGGGGTGGATTGGACGTTGAAGTGGAGAAAGTCACAGA GGAAGTCCAAAAGGGACTAG
Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase
Products: NA
Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]
Number of amino acids: Translated: 761; Mature: 761
Protein sequence:
>761_residues MRRAGMKTAVAGYPRIGTLRELKFALEKYFRKEISADELTQTAKELRKTHWLIQKEAGIDYITSNDFSYYDIVLDTAFLL NIISERYNELEVSELDKYLAMARGYQGENGDVKALAMKKWFNTNYHYIVPEVEDSTQIRLTGNKLWAEYAEAKELGIETK PVITGVYTLFKLCRFTGKKKADDFINAFIEAYKDVYSKCEAVGIQWLQFDEPALVQDMTEEDRELFVKMYSDILGKKKSC KVLLQTYFGDVRDVYEDIVKLSFDGIGLDFIEGKKTAELIEKYGFPKDTILFAGLVNGKNIWKNHYEKTLNVLKKLEDKG IQTVLSTSCSLQHVPYTLKHENKLSDEYLNYFAFAEEKLVELKELSVLAECGNIEEDERFKTNRKLFAGTRKCDNESVKK RLAEVTEADYRRLPARSERQQLQKKEFALPKLPTTTIGSFPQTKDVKANRSAFRKGEISEEQYVEFNKKKIEECVRWQEK IGLDVLVHGEYERNDMVEYFGEALGGFLFTEKAWVQSYGTRCVKPPVIWGDVYRKKPITVEWSVYAQSLTDKIMKGMLTG PVTILNWSFPREDITIKESISQIALAIRDEVLDLEANGIKIIQIDEAALREKLPLRKSDWNTEYLDFSIPAFRLTASGVK PETQIHTHMCYSEFTDIIPAIDDMDADVITFEASRSDLQILDSLRENNFETEVGPGVYDIHSPRIPSVEEITRAIKIMLT KIDKDKLWVNPDCGLKTRGVPETEASLKNMVKAAEIIRAGL
Sequences:
>Translated_761_residues MRRAGMKTAVAGYPRIGTLRELKFALEKYFRKEISADELTQTAKELRKTHWLIQKEAGIDYITSNDFSYYDIVLDTAFLL NIISERYNELEVSELDKYLAMARGYQGENGDVKALAMKKWFNTNYHYIVPEVEDSTQIRLTGNKLWAEYAEAKELGIETK PVITGVYTLFKLCRFTGKKKADDFINAFIEAYKDVYSKCEAVGIQWLQFDEPALVQDMTEEDRELFVKMYSDILGKKKSC KVLLQTYFGDVRDVYEDIVKLSFDGIGLDFIEGKKTAELIEKYGFPKDTILFAGLVNGKNIWKNHYEKTLNVLKKLEDKG IQTVLSTSCSLQHVPYTLKHENKLSDEYLNYFAFAEEKLVELKELSVLAECGNIEEDERFKTNRKLFAGTRKCDNESVKK RLAEVTEADYRRLPARSERQQLQKKEFALPKLPTTTIGSFPQTKDVKANRSAFRKGEISEEQYVEFNKKKIEECVRWQEK IGLDVLVHGEYERNDMVEYFGEALGGFLFTEKAWVQSYGTRCVKPPVIWGDVYRKKPITVEWSVYAQSLTDKIMKGMLTG PVTILNWSFPREDITIKESISQIALAIRDEVLDLEANGIKIIQIDEAALREKLPLRKSDWNTEYLDFSIPAFRLTASGVK PETQIHTHMCYSEFTDIIPAIDDMDADVITFEASRSDLQILDSLRENNFETEVGPGVYDIHSPRIPSVEEITRAIKIMLT KIDKDKLWVNPDCGLKTRGVPETEASLKNMVKAAEIIRAGL >Mature_761_residues MRRAGMKTAVAGYPRIGTLRELKFALEKYFRKEISADELTQTAKELRKTHWLIQKEAGIDYITSNDFSYYDIVLDTAFLL NIISERYNELEVSELDKYLAMARGYQGENGDVKALAMKKWFNTNYHYIVPEVEDSTQIRLTGNKLWAEYAEAKELGIETK PVITGVYTLFKLCRFTGKKKADDFINAFIEAYKDVYSKCEAVGIQWLQFDEPALVQDMTEEDRELFVKMYSDILGKKKSC KVLLQTYFGDVRDVYEDIVKLSFDGIGLDFIEGKKTAELIEKYGFPKDTILFAGLVNGKNIWKNHYEKTLNVLKKLEDKG IQTVLSTSCSLQHVPYTLKHENKLSDEYLNYFAFAEEKLVELKELSVLAECGNIEEDERFKTNRKLFAGTRKCDNESVKK RLAEVTEADYRRLPARSERQQLQKKEFALPKLPTTTIGSFPQTKDVKANRSAFRKGEISEEQYVEFNKKKIEECVRWQEK IGLDVLVHGEYERNDMVEYFGEALGGFLFTEKAWVQSYGTRCVKPPVIWGDVYRKKPITVEWSVYAQSLTDKIMKGMLTG PVTILNWSFPREDITIKESISQIALAIRDEVLDLEANGIKIIQIDEAALREKLPLRKSDWNTEYLDFSIPAFRLTASGVK PETQIHTHMCYSEFTDIIPAIDDMDADVITFEASRSDLQILDSLRENNFETEVGPGVYDIHSPRIPSVEEITRAIKIMLT KIDKDKLWVNPDCGLKTRGVPETEASLKNMVKAAEIIRAGL
Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]
COG id: COG0620
COG function: function code E; Methionine synthase II (cobalamin-independent)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]
Homologues:
Organism=Escherichia coli, GI2367304, Length=756, Percent_Identity=46.031746031746, Blast_Score=654, Evalue=0.0, Organism=Saccharomyces cerevisiae, GI6320936, Length=775, Percent_Identity=43.4838709677419, Blast_Score=607, Evalue=1e-174,
Paralogues:
None
Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013215 - InterPro: IPR006276 - InterPro: IPR002629 [H]
Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]
EC number: =2.1.1.14 [H]
Molecular weight: Translated: 87475; Mature: 87475
Theoretical pI: Translated: 5.43; Mature: 5.43
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 3.0 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 3.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRRAGMKTAVAGYPRIGTLRELKFALEKYFRKEISADELTQTAKELRKTHWLIQKEAGID CCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC YITSNDFSYYDIVLDTAFLLNIISERYNELEVSELDKYLAMARGYQGENGDVKALAMKKW EEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHH FNTNYHYIVPEVEDSTQIRLTGNKLWAEYAEAKELGIETKPVITGVYTLFKLCRFTGKKK HCCCCEEEEECCCCCCEEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC ADDFINAFIEAYKDVYSKCEAVGIQWLQFDEPALVQDMTEEDRELFVKMYSDILGKKKSC HHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH KVLLQTYFGDVRDVYEDIVKLSFDGIGLDFIEGKKTAELIEKYGFPKDTILFAGLVNGKN HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHCCCCHHEEEEEECCCHH IWKNHYEKTLNVLKKLEDKGIQTVLSTSCSLQHVPYTLKHENKLSDEYLNYFAFAEEKLV HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHH ELKELSVLAECGNIEEDERFKTNRKLFAGTRKCDNESVKKRLAEVTEADYRRLPARSERQ HHHHHHHHHHCCCCCHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH QLQKKEFALPKLPTTTIGSFPQTKDVKANRSAFRKGEISEEQYVEFNKKKIEECVRWQEK HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH IGLDVLVHGEYERNDMVEYFGEALGGFLFTEKAWVQSYGTRCVKPPVIWGDVYRKKPITV CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHCCCCCEE EWSVYAQSLTDKIMKGMLTGPVTILNWSFPREDITIKESISQIALAIRDEVLDLEANGIK EHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCEE IIQIDEAALREKLPLRKSDWNTEYLDFSIPAFRLTASGVKPETQIHTHMCYSEFTDIIPA EEEECHHHHHHCCCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC IDDMDADVITFEASRSDLQILDSLRENNFETEVGPGVYDIHSPRIPSVEEITRAIKIMLT CCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHH KIDKDKLWVNPDCGLKTRGVPETEASLKNMVKAAEIIRAGL HCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC >Mature Secondary Structure MRRAGMKTAVAGYPRIGTLRELKFALEKYFRKEISADELTQTAKELRKTHWLIQKEAGID CCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC YITSNDFSYYDIVLDTAFLLNIISERYNELEVSELDKYLAMARGYQGENGDVKALAMKKW EEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHH FNTNYHYIVPEVEDSTQIRLTGNKLWAEYAEAKELGIETKPVITGVYTLFKLCRFTGKKK HCCCCEEEEECCCCCCEEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC ADDFINAFIEAYKDVYSKCEAVGIQWLQFDEPALVQDMTEEDRELFVKMYSDILGKKKSC HHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH KVLLQTYFGDVRDVYEDIVKLSFDGIGLDFIEGKKTAELIEKYGFPKDTILFAGLVNGKN HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHCCCCHHEEEEEECCCHH IWKNHYEKTLNVLKKLEDKGIQTVLSTSCSLQHVPYTLKHENKLSDEYLNYFAFAEEKLV HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHH ELKELSVLAECGNIEEDERFKTNRKLFAGTRKCDNESVKKRLAEVTEADYRRLPARSERQ HHHHHHHHHHCCCCCHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH QLQKKEFALPKLPTTTIGSFPQTKDVKANRSAFRKGEISEEQYVEFNKKKIEECVRWQEK HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH IGLDVLVHGEYERNDMVEYFGEALGGFLFTEKAWVQSYGTRCVKPPVIWGDVYRKKPITV CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHCCCCCEE EWSVYAQSLTDKIMKGMLTGPVTILNWSFPREDITIKESISQIALAIRDEVLDLEANGIK EHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCEE IIQIDEAALREKLPLRKSDWNTEYLDFSIPAFRLTASGVKPETQIHTHMCYSEFTDIIPA EEEECHHHHHHCCCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC IDDMDADVITFEASRSDLQILDSLRENNFETEVGPGVYDIHSPRIPSVEEITRAIKIMLT CCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHH KIDKDKLWVNPDCGLKTRGVPETEASLKNMVKAAEIIRAGL HCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA