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The map label for this gene is mdeA [H]

Identifier: 238916581

GI number: 238916581

Start: 645259

End: 646494

Strand: Direct

Name: mdeA [H]

Synonym: EUBELI_00638

Alternate gene names: 238916581

Gene position: 645259-646494 (Clockwise)

Preceding gene: 238916580

Following gene: 238916582

Centisome position: 30.09

GC content: 38.27

Gene sequence:

>1236_bases
ATGAGTGGTTTTAACACATCACTGCTGCATGATGGCGTTGACGATTATCCTAACGGAGCAACACTTGTTCCTATATATCA
GTCAAGCGCATTTAAGCATGAGAGTGCAGAAGAACTGGAGAGAATATTTGATAATAAGGCGATGGGATTCTCATATACGA
GAATCAATAATCCGACGATAGAAGCATTTGAAAAAAGAATTACCAAGCTGGAAAAAGGATTTTCAAGTGTAGCGTGTTCT
TCCGGAATGGCGGCAATATTTAATTCTATAGCTAATATTGTAAGACAGGGTGATGAGGTTGTTGCTTCATGTGGATTATA
TGGTGGAACAGTCGAACTGTTTGAAGACTTAGAAAGCTTTGGCATTACAGTGAAATATGTGGCTGATAATAAGCCAGAGT
GTTTTGAAGAACTTATAACAGATAAGACAAGAGTTGTATTTGCAGAGACAATCGGTAATCCCAAGCTTGATGTAACAGAT
ATTAAGGCAGTAGCGGATGTTGCACATGCACATGGAATTCCGCTTATAGTTGATAATACGGTATCAACGCCATATCTTAT
ACAGCCTATAACTCTTGGCGCAGATGTGGTAGTTAATTCATCATCAAAGTACATTAATGGAAGCAGTGATGCGATAAGCG
GAATACTTACTTTTAATGGAAAGTTCAAGTTTGATAAAGAAAAGTATCCGGGACTTAAACCATATTTGAAATTCGGTCCA
ATGGCATATATAATTAAACTTCGTAACGGATTATTCAGAAATACTGGTGGTTGTCTTTCACCACAGAATGCTTTTCTTAA
TAACCTGGGACTTGAGACATTAGGGCTTAGAATGGAGAGACACTGTTCTAATGCGTATGGACTTGCCAGATACTTAGACA
CATTTGAAGGAATTACAGTTAATTATCCGGGACTTGAGAGCAGTCCGTATCATGAAGTGGCTGATAAGCAGTTTACTAAA
GGCTATGGTGCGATAATAACCTTCAGGGTTGGAAGCAAGGAAAGAGCATTTAAGATAATTAATGCATTAAAGATTCCTAA
TATAGTTTCTAACATAGGAGATACCAAGACACTGGTTATCCACCCTGCTTCAACGATTGCACTTCATCTAAGCGGCAAGG
ATAAAGAAGCTTCAGGAGTGTATGACGATCTGATAAGAGTCAGCGTAGGAATAGAGGATATAGATGATCTTAAGGAAGAT
TTCAGTGCAGCCTTAGAGTCTACTGATAATGAATAG

Upstream 100 bases:

>100_bases
CAAATAACCTATTGACATACTAGGTAAAAGGGTTTAATATATGGACAGTTCAATAAATATTGAATGTTCATCGCATAACA
GATGAAAGGATGAGAAATAT

Downstream 100 bases:

>100_bases
TGCATGGCACGGAAATGAGGATATTATGGCAGAGAAAGTTGATTATGGAACACTTAAGAAGGGCGGATTCATGAGACAGA
AGCAGAAGAATAACTTCTCA

Product: O-acetylhomoserine (thiol)-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MSGFNTSLLHDGVDDYPNGATLVPIYQSSAFKHESAEELERIFDNKAMGFSYTRINNPTIEAFEKRITKLEKGFSSVACS
SGMAAIFNSIANIVRQGDEVVASCGLYGGTVELFEDLESFGITVKYVADNKPECFEELITDKTRVVFAETIGNPKLDVTD
IKAVADVAHAHGIPLIVDNTVSTPYLIQPITLGADVVVNSSSKYINGSSDAISGILTFNGKFKFDKEKYPGLKPYLKFGP
MAYIIKLRNGLFRNTGGCLSPQNAFLNNLGLETLGLRMERHCSNAYGLARYLDTFEGITVNYPGLESSPYHEVADKQFTK
GYGAIITFRVGSKERAFKIINALKIPNIVSNIGDTKTLVIHPASTIALHLSGKDKEASGVYDDLIRVSVGIEDIDDLKED
FSAALESTDNE

Sequences:

>Translated_411_residues
MSGFNTSLLHDGVDDYPNGATLVPIYQSSAFKHESAEELERIFDNKAMGFSYTRINNPTIEAFEKRITKLEKGFSSVACS
SGMAAIFNSIANIVRQGDEVVASCGLYGGTVELFEDLESFGITVKYVADNKPECFEELITDKTRVVFAETIGNPKLDVTD
IKAVADVAHAHGIPLIVDNTVSTPYLIQPITLGADVVVNSSSKYINGSSDAISGILTFNGKFKFDKEKYPGLKPYLKFGP
MAYIIKLRNGLFRNTGGCLSPQNAFLNNLGLETLGLRMERHCSNAYGLARYLDTFEGITVNYPGLESSPYHEVADKQFTK
GYGAIITFRVGSKERAFKIINALKIPNIVSNIGDTKTLVIHPASTIALHLSGKDKEASGVYDDLIRVSVGIEDIDDLKED
FSAALESTDNE
>Mature_410_residues
SGFNTSLLHDGVDDYPNGATLVPIYQSSAFKHESAEELERIFDNKAMGFSYTRINNPTIEAFEKRITKLEKGFSSVACSS
GMAAIFNSIANIVRQGDEVVASCGLYGGTVELFEDLESFGITVKYVADNKPECFEELITDKTRVVFAETIGNPKLDVTDI
KAVADVAHAHGIPLIVDNTVSTPYLIQPITLGADVVVNSSSKYINGSSDAISGILTFNGKFKFDKEKYPGLKPYLKFGPM
AYIIKLRNGLFRNTGGCLSPQNAFLNNLGLETLGLRMERHCSNAYGLARYLDTFEGITVNYPGLESSPYHEVADKQFTKG
YGAIITFRVGSKERAFKIINALKIPNIVSNIGDTKTLVIHPASTIALHLSGKDKEASGVYDDLIRVSVGIEDIDDLKEDF
SAALESTDNE

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=392, Percent_Identity=32.6530612244898, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI299473759, Length=392, Percent_Identity=30.1020408163265, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI25453487, Length=391, Percent_Identity=27.6214833759591, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1790375, Length=391, Percent_Identity=29.4117647058824, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1789383, Length=412, Percent_Identity=24.7572815533981, Blast_Score=109, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71980708, Length=391, Percent_Identity=34.0153452685422, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17538055, Length=398, Percent_Identity=33.6683417085427, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17560056, Length=400, Percent_Identity=33.25, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=38.3908045977011, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6319307, Length=416, Percent_Identity=33.6538461538462, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6321254, Length=433, Percent_Identity=27.2517321016166, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6321170, Length=326, Percent_Identity=26.3803680981595, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321904, Length=420, Percent_Identity=26.1904761904762, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922554, Length=412, Percent_Identity=32.0388349514563, Blast_Score=189, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 44867; Mature: 44736

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFNTSLLHDGVDDYPNGATLVPIYQSSAFKHESAEELERIFDNKAMGFSYTRINNPTI
CCCCCCHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCHH
EAFEKRITKLEKGFSSVACSSGMAAIFNSIANIVRQGDEVVASCGLYGGTVELFEDLESF
HHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHH
GITVKYVADNKPECFEELITDKTRVVFAETIGNPKLDVTDIKAVADVAHAHGIPLIVDNT
CCEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECC
VSTPYLIQPITLGADVVVNSSSKYINGSSDAISGILTFNGKFKFDKEKYPGLKPYLKFGP
CCCCEEEEEEECCCEEEEECCCCCCCCCHHHEEEEEEECCEEEECCCCCCCCCCHHHHCC
MAYIIKLRNGLFRNTGGCLSPQNAFLNNLGLETLGLRMERHCSNAYGLARYLDTFEGITV
HHHHHHHHCCHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
NYPGLESSPYHEVADKQFTKGYGAIITFRVGSKERAFKIINALKIPNIVSNIGDTKTLVI
ECCCCCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCHHHHHCCCCEEEEE
HPASTIALHLSGKDKEASGVYDDLIRVSVGIEDIDDLKEDFSAALESTDNE
ECCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SGFNTSLLHDGVDDYPNGATLVPIYQSSAFKHESAEELERIFDNKAMGFSYTRINNPTI
CCCCCHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCHH
EAFEKRITKLEKGFSSVACSSGMAAIFNSIANIVRQGDEVVASCGLYGGTVELFEDLESF
HHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHH
GITVKYVADNKPECFEELITDKTRVVFAETIGNPKLDVTDIKAVADVAHAHGIPLIVDNT
CCEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECC
VSTPYLIQPITLGADVVVNSSSKYINGSSDAISGILTFNGKFKFDKEKYPGLKPYLKFGP
CCCCEEEEEEECCCEEEEECCCCCCCCCHHHEEEEEEECCEEEECCCCCCCCCCHHHHCC
MAYIIKLRNGLFRNTGGCLSPQNAFLNNLGLETLGLRMERHCSNAYGLARYLDTFEGITV
HHHHHHHHCCHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
NYPGLESSPYHEVADKQFTKGYGAIITFRVGSKERAFKIINALKIPNIVSNIGDTKTLVI
ECCCCCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCHHHHHCCCCEEEEE
HPASTIALHLSGKDKEASGVYDDLIRVSVGIEDIDDLKEDFSAALESTDNE
ECCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]