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The map label for this gene is purN [H]

Identifier: 238916493

GI number: 238916493

Start: 554783

End: 555379

Strand: Direct

Name: purN [H]

Synonym: EUBELI_00550

Alternate gene names: 238916493

Gene position: 554783-555379 (Clockwise)

Preceding gene: 238916492

Following gene: 238916494

Centisome position: 25.87

GC content: 40.2

Gene sequence:

>597_bases
ATGCTAAGAGTTGCAGTTATGGTTTCCGGAGGCGGAACTAATCTTCAGGCTATTATCGATGCGGTTAAGGATGGAACTAT
CACTAACACAGAGCTTGTAGCAGTTATAAGCAATAATGCCGGAGCATATGCACTCACAAGAGCTAAGGATAACAATATTC
CAGCATATTGCATATCACCTAAGGATTATGAAAGCAGAGATGCATTTAACGATGCGCTGCTTGATAAAGTGAATGAGCTT
AATGTTGACCTTATAGTACTCGCAGGATTCCTTGTAAGAATCCCAGAGAAAATGGTTCATCAGTACAGCCACAGAATTAT
TAATATTCATCCATCACTTATACCTTCCTTCTGCGGAGTTGGTTTCTATGGACTCAAGGTTCATGAAGCAGCTCTGGCAA
AGGGTGTTAAGGTATCAGGTGCAACTGTGCATTATGTAGATGAAGGAATGGATACAGGAGAGATTATTTTCCAGAAGGCA
GTTGATGTACTTGATGGTGATACTCCTGAAACTCTTCAGAGAAGAATTATGGAGCAGGCTGAATGGAAGCTTCTTCCTAA
GGCAATTAACAAGATTGCCAATGAACATGTTAATTAA

Upstream 100 bases:

>100_bases
ATCCTGCTGATGTTGATACAGTTATGGAAGCAGTTAAGGCTGCAGGCGAGACTCCATATGTAATCGGAAGCATTGAAGCT
GGCGAGAAGGGAGTTACTTT

Downstream 100 bases:

>100_bases
TGATTACTGACTAAGTATATAGATAATGATATGCGGAGGAATTAAGATGAAAGTATTAGTTGTAGGCAGCGGCGGAAGAG
AGCATGCAATATGCTGGAAG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MLRVAVMVSGGGTNLQAIIDAVKDGTITNTELVAVISNNAGAYALTRAKDNNIPAYCISPKDYESRDAFNDALLDKVNEL
NVDLIVLAGFLVRIPEKMVHQYSHRIINIHPSLIPSFCGVGFYGLKVHEAALAKGVKVSGATVHYVDEGMDTGEIIFQKA
VDVLDGDTPETLQRRIMEQAEWKLLPKAINKIANEHVN

Sequences:

>Translated_198_residues
MLRVAVMVSGGGTNLQAIIDAVKDGTITNTELVAVISNNAGAYALTRAKDNNIPAYCISPKDYESRDAFNDALLDKVNEL
NVDLIVLAGFLVRIPEKMVHQYSHRIINIHPSLIPSFCGVGFYGLKVHEAALAKGVKVSGATVHYVDEGMDTGEIIFQKA
VDVLDGDTPETLQRRIMEQAEWKLLPKAINKIANEHVN
>Mature_198_residues
MLRVAVMVSGGGTNLQAIIDAVKDGTITNTELVAVISNNAGAYALTRAKDNNIPAYCISPKDYESRDAFNDALLDKVNEL
NVDLIVLAGFLVRIPEKMVHQYSHRIINIHPSLIPSFCGVGFYGLKVHEAALAKGVKVSGATVHYVDEGMDTGEIIFQKA
VDVLDGDTPETLQRRIMEQAEWKLLPKAINKIANEHVN

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=196, Percent_Identity=40.3061224489796, Blast_Score=140, Evalue=6e-34,
Organism=Homo sapiens, GI209869995, Length=196, Percent_Identity=40.3061224489796, Blast_Score=140, Evalue=6e-34,
Organism=Homo sapiens, GI209869993, Length=196, Percent_Identity=40.3061224489796, Blast_Score=140, Evalue=6e-34,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=35.7512953367876, Blast_Score=124, Evalue=3e-30,
Organism=Escherichia coli, GI1787483, Length=195, Percent_Identity=28.2051282051282, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=192, Percent_Identity=35.9375, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320616, Length=193, Percent_Identity=33.6787564766839, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24582400, Length=193, Percent_Identity=43.0051813471503, Blast_Score=152, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21613; Mature: 21613

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVAVMVSGGGTNLQAIIDAVKDGTITNTELVAVISNNAGAYALTRAKDNNIPAYCISP
CEEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEECC
KDYESRDAFNDALLDKVNELNVDLIVLAGFLVRIPEKMVHQYSHRIINIHPSLIPSFCGV
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCEEEEECHHHHHHHHCC
GFYGLKVHEAALAKGVKVSGATVHYVDEGMDTGEIIFQKAVDVLDGDTPETLQRRIMEQA
CEEHHHHHHHHHHCCCEECCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EWKLLPKAINKIANEHVN
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLRVAVMVSGGGTNLQAIIDAVKDGTITNTELVAVISNNAGAYALTRAKDNNIPAYCISP
CEEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEECC
KDYESRDAFNDALLDKVNELNVDLIVLAGFLVRIPEKMVHQYSHRIINIHPSLIPSFCGV
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCEEEEECHHHHHHHHCC
GFYGLKVHEAALAKGVKVSGATVHYVDEGMDTGEIIFQKAVDVLDGDTPETLQRRIMEQA
CEEHHHHHHHHHHCCCEECCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EWKLLPKAINKIANEHVN
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA