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The map label for this gene is purD [H]

Identifier: 238916494

GI number: 238916494

Start: 555405

End: 556700

Strand: Direct

Name: purD [H]

Synonym: EUBELI_00551

Alternate gene names: 238916494

Gene position: 555405-556700 (Clockwise)

Preceding gene: 238916493

Following gene: 238916495

Centisome position: 25.9

GC content: 41.82

Gene sequence:

>1296_bases
ATGATATGCGGAGGAATTAAGATGAAAGTATTAGTTGTAGGCAGCGGCGGAAGAGAGCATGCAATATGCTGGAAGTTAAG
CAAGAGTCCTAAGGTTGACAAGATATACTGTGCACCGGGTAATGCGGGAATTGCAGAGCTTGCAGAGTGTGTTGATATTA
AGGCAATGGAGTTTGAGAAGCTTGTAGCATTTGCTAAGGAAAACAGTATTGACCTTACTGTTATCGGTATGGACGACCCA
CTTGTCGGCGGTGTCGTTGATGTATTCGAGGCAGAGGGCTTAAGAGTATTCGGACCAAGAAAGAATGCAGCTATTCTTGA
AGGATCTAAGGCATTTTCTAAGGACCTTATGAAGAAATACAATATTCCAACAGCAGCTTATGAAACATTTACATCAGCAG
AAGAGGCCAAGAAGTACCTTGAGACTGCTGAGTATCCTATCGTTCTTAAGGCTGACGGACTTGCACTTGGCAAGGGTGTT
CTCATCTGTGAGAACAAAGAGGATGCACTTGCAGGTGTTGATGAGCTTATGCTTGACAAGAAATTCGGAACAGCAGGTGA
TACAATTGTCATTGAGGAATTCATGACAGGCCGTGAGGTTTCAGTACTTTCATTTGTTGATGGTGATACAATTAAGATTA
TGTCATCAGCTCAGGATCATAAGAGAGCTAAGGATGGTGACCAGGGCCTTAACACAGGCGGAATGGGTAACTTCTCACCA
TCACCTTTCTACACTAAGGAAGTTGATGAGTTCTGTAAGAAATACATTTATCAGGCAACTGTTGATGCAATGAAGAGTGA
AGGCAGACCATTTAAGGGAGTTATCTTCTTTGGACTTATGCTTACTCCAAAGGGACCAAAGGTTCTTGAGTACAATGCAA
GATTTGGTGATCCTGAGGCACAGGTTGTTCTTCCTAGAATGAAGAATGATATTGTTGATGTATTTGAGGCTTGTATTGAT
GGAACTCTTGATAAGGTTGACCTTCAGTTTGAAGATAACGCATGTGTATGTGTAATTCTCGCATCTGACGGATATCCACT
CGCATATGAGAAGGGCTTCGAGATTAAGGGAATGGAAAACTTCAAGGGTAAGGATGATTATTTCTTATTCCATGCAGGTT
CTAAATTCAACGAAGAAGGCAAGGTTGTAACTAACGGAGGACGAGTTCTCGGTGTTACAGCACTTGGCAAGGACCTTAAG
GAAGCAAGAGCCAAGGCTTATGAAGCTACTGAATGGGTAGATTTCGATAACAAATATATGAGACATGATATTGGTAAGGC
AATTGATGAAGCTTAA

Upstream 100 bases:

>100_bases
AGAATTATGGAGCAGGCTGAATGGAAGCTTCTTCCTAAGGCAATTAACAAGATTGCCAATGAACATGTTAATTAATGATT
ACTGACTAAGTATATAGATA

Downstream 100 bases:

>100_bases
TTAGTAACTGTTAAGAGTCAGGCAAAATGCGAAGCATTTTCGATACTGGCGGAAAATAGTTAGTATTAAAGAAAATGAGG
GCAGATGGCTGGAGAATACC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MICGGIKMKVLVVGSGGREHAICWKLSKSPKVDKIYCAPGNAGIAELAECVDIKAMEFEKLVAFAKENSIDLTVIGMDDP
LVGGVVDVFEAEGLRVFGPRKNAAILEGSKAFSKDLMKKYNIPTAAYETFTSAEEAKKYLETAEYPIVLKADGLALGKGV
LICENKEDALAGVDELMLDKKFGTAGDTIVIEEFMTGREVSVLSFVDGDTIKIMSSAQDHKRAKDGDQGLNTGGMGNFSP
SPFYTKEVDEFCKKYIYQATVDAMKSEGRPFKGVIFFGLMLTPKGPKVLEYNARFGDPEAQVVLPRMKNDIVDVFEACID
GTLDKVDLQFEDNACVCVILASDGYPLAYEKGFEIKGMENFKGKDDYFLFHAGSKFNEEGKVVTNGGRVLGVTALGKDLK
EARAKAYEATEWVDFDNKYMRHDIGKAIDEA

Sequences:

>Translated_431_residues
MICGGIKMKVLVVGSGGREHAICWKLSKSPKVDKIYCAPGNAGIAELAECVDIKAMEFEKLVAFAKENSIDLTVIGMDDP
LVGGVVDVFEAEGLRVFGPRKNAAILEGSKAFSKDLMKKYNIPTAAYETFTSAEEAKKYLETAEYPIVLKADGLALGKGV
LICENKEDALAGVDELMLDKKFGTAGDTIVIEEFMTGREVSVLSFVDGDTIKIMSSAQDHKRAKDGDQGLNTGGMGNFSP
SPFYTKEVDEFCKKYIYQATVDAMKSEGRPFKGVIFFGLMLTPKGPKVLEYNARFGDPEAQVVLPRMKNDIVDVFEACID
GTLDKVDLQFEDNACVCVILASDGYPLAYEKGFEIKGMENFKGKDDYFLFHAGSKFNEEGKVVTNGGRVLGVTALGKDLK
EARAKAYEATEWVDFDNKYMRHDIGKAIDEA
>Mature_431_residues
MICGGIKMKVLVVGSGGREHAICWKLSKSPKVDKIYCAPGNAGIAELAECVDIKAMEFEKLVAFAKENSIDLTVIGMDDP
LVGGVVDVFEAEGLRVFGPRKNAAILEGSKAFSKDLMKKYNIPTAAYETFTSAEEAKKYLETAEYPIVLKADGLALGKGV
LICENKEDALAGVDELMLDKKFGTAGDTIVIEEFMTGREVSVLSFVDGDTIKIMSSAQDHKRAKDGDQGLNTGGMGNFSP
SPFYTKEVDEFCKKYIYQATVDAMKSEGRPFKGVIFFGLMLTPKGPKVLEYNARFGDPEAQVVLPRMKNDIVDVFEACID
GTLDKVDLQFEDNACVCVILASDGYPLAYEKGFEIKGMENFKGKDDYFLFHAGSKFNEEGKVVTNGGRVLGVTALGKDLK
EARAKAYEATEWVDFDNKYMRHDIGKAIDEA

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=421, Percent_Identity=44.1805225653207, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI209869995, Length=421, Percent_Identity=44.1805225653207, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI209869993, Length=421, Percent_Identity=44.1805225653207, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI28416899, Length=421, Percent_Identity=44.1805225653207, Blast_Score=362, Evalue=1e-100,
Organism=Escherichia coli, GI1790438, Length=430, Percent_Identity=45.8139534883721, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17567511, Length=428, Percent_Identity=39.018691588785, Blast_Score=281, Evalue=5e-76,
Organism=Saccharomyces cerevisiae, GI6321203, Length=435, Percent_Identity=42.5287356321839, Blast_Score=335, Evalue=9e-93,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=45.5813953488372, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24582400, Length=414, Percent_Identity=46.6183574879227, Blast_Score=359, Evalue=2e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 47140; Mature: 47140

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICGGIKMKVLVVGSGGREHAICWKLSKSPKVDKIYCAPGNAGIAELAECVDIKAMEFEK
CCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHCCHHHHHHH
LVAFAKENSIDLTVIGMDDPLVGGVVDVFEAEGLRVFGPRKNAAILEGSKAFSKDLMKKY
HHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCCEEEECCCCCCEEEECCHHHHHHHHHHH
NIPTAAYETFTSAEEAKKYLETAEYPIVLKADGLALGKGVLICENKEDALAGVDELMLDK
CCCHHHHHHHCCHHHHHHHHHHCCCCEEEEECCEEECCEEEEECCCCHHHHHHHHHHHHC
KFGTAGDTIVIEEFMTGREVSVLSFVDGDTIKIMSSAQDHKRAKDGDQGLNTGGMGNFSP
CCCCCCCEEEEEHHHCCCCEEEEEEECCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCCC
SPFYTKEVDEFCKKYIYQATVDAMKSEGRPFKGVIFFGLMLTPKGPKVLEYNARFGDPEA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCEEEECCCCCCCCC
QVVLPRMKNDIVDVFEACIDGTLDKVDLQFEDNACVCVILASDGYPLAYEKGFEIKGMEN
EEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCEEECCCCEECCCCC
FKGKDDYFLFHAGSKFNEEGKVVTNGGRVLGVTALGKDLKEARAKAYEATEWVDFDNKYM
CCCCCCEEEEECCCCCCCCCCEEECCCEEEEEHHHHHHHHHHHHHHHCCHHEECCCCHHH
RHDIGKAIDEA
HHHHHHHHCCC
>Mature Secondary Structure
MICGGIKMKVLVVGSGGREHAICWKLSKSPKVDKIYCAPGNAGIAELAECVDIKAMEFEK
CCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHCCHHHHHHH
LVAFAKENSIDLTVIGMDDPLVGGVVDVFEAEGLRVFGPRKNAAILEGSKAFSKDLMKKY
HHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCCEEEECCCCCCEEEECCHHHHHHHHHHH
NIPTAAYETFTSAEEAKKYLETAEYPIVLKADGLALGKGVLICENKEDALAGVDELMLDK
CCCHHHHHHHCCHHHHHHHHHHCCCCEEEEECCEEECCEEEEECCCCHHHHHHHHHHHHC
KFGTAGDTIVIEEFMTGREVSVLSFVDGDTIKIMSSAQDHKRAKDGDQGLNTGGMGNFSP
CCCCCCCEEEEEHHHCCCCEEEEEEECCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCCC
SPFYTKEVDEFCKKYIYQATVDAMKSEGRPFKGVIFFGLMLTPKGPKVLEYNARFGDPEA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCEEEECCCCCCCCC
QVVLPRMKNDIVDVFEACIDGTLDKVDLQFEDNACVCVILASDGYPLAYEKGFEIKGMEN
EEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCEEECCCCEECCCCC
FKGKDDYFLFHAGSKFNEEGKVVTNGGRVLGVTALGKDLKEARAKAYEATEWVDFDNKYM
CCCCCCEEEEECCCCCCCCCCEEECCCEEEEEHHHHHHHHHHHHHHHCCHHEECCCCHHH
RHDIGKAIDEA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]