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The map label for this gene is thiH [H]

Identifier: 238916422

GI number: 238916422

Start: 486633

End: 487892

Strand: Direct

Name: thiH [H]

Synonym: EUBELI_00478

Alternate gene names: 238916422

Gene position: 486633-487892 (Clockwise)

Preceding gene: 238916421

Following gene: 238916423

Centisome position: 22.7

GC content: 38.1

Gene sequence:

>1260_bases
ATGAACGAAGAAAATCAGGTATATTTTATAGACAGTGACCAGCTTCCACCAGCCGCGTTAGAGAGAAAGCATAAGTTGGA
GCAGGATCCGTCATTCAGAACAGATTACATGAAATATCTTGATGACATGGAAATAATTGATTCAGATATTAAGGACAAAG
TATTAAAGGCAATGGATGAGTTTGATTATGATTCATACACAGCAGCAGATGTTGAGAGGGCTTTAAGCAAAGATACCTGC
AATATTGAGGATTTTAAGGCATTGCTTTCGCCGGCTGCAGCACCTTACTTAGAGCAGATGGCAAGAAAGGCAGAGAATGA
AACTAAGAAACATTTTGGCAATACAGTGTATATATTTACGCCAATATATATAGCCAATTATTGCCAGAATTACTGCATAT
ACTGTGGATTCAACTGCTACAATAACATTCACAGAAAGAAACTTTCATTTGAGGAAATTGAGCATGAGATGAAAGTTATT
GCTGATACAGGAATGGAAGAAATTCTGATACTGACAGGAGAGAGTAGAAAATATTCAGACGTTGAATACATTGGCGAATC
AGTTAAGATAGCAAGAAAATATTTCAAGAATATCGGAATAGAGATATATCCTGTAAATGTGGATGAATACCGCTATCTTC
ATGAGTGCGGTGTTGATTATGTAACAGTATTCCAGGAGACATATAATCCTGTCAAATATGAGACATTACATTTATTAGGA
CATAAGAGAGTATGGCCATACAGATTCGATGCACAGGAAAGAGCACTTATTGCAGGTATGAGAGGTGCAGGATTTTCAGC
ATTGTTAGGGCTTGATGATTTCAGAAAAGATGCACTGGCAACGGCACTTCATGTATATTATCTTAACAGAAAATACCCTC
ATGCAGAGCTGAGTCTTAGCTGTCCGAGACTCCGTCCGATAATCAATAATGATAAGATTAATCCAAGAGATGTCGGTGAA
AAGCAGTTGTGCCAGGTACTTTGTGCGTACAGATTATTCCTTCCATTTGTTGGAATAACAGTTTCAAGCCGTGAGAGTGC
GCAGTTTAGAAATGGTATAACCAAGATATGTGCCACTAAAGTTTCAGCGGGTGTTTCAACAGGAATCGGTGACCATGAAT
CTAAATATACAGGAAAAAAAGATGGTGATGCCGGAGATGAACAGTTTGAAATCAATGATAACCGTTCATTTGACAGAATG
TATAAGGATATGGAGGATGGCGGGCTTCAGCCAGTCGTGAATGAATACATTTATGTATAG

Upstream 100 bases:

>100_bases
ATTGCAAGAGATACTGGCGGGATAATTTCAGCGGAAGCATAACTTCGGGGAAGTATTGCTTGACGAAATATGAGAATATA
ATAGAAAAGAAGGCAGAATA

Downstream 100 bases:

>100_bases
AAAATATATATGTATAACGAACAAGCACCTTGTGAGTGGCAGCGGTGGTGCAGAGTGTACTATGAATGAGTACATTATGC
AGCTTGAAAAATGTGTGGCA

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNEENQVYFIDSDQLPPAALERKHKLEQDPSFRTDYMKYLDDMEIIDSDIKDKVLKAMDEFDYDSYTAADVERALSKDTC
NIEDFKALLSPAAAPYLEQMARKAENETKKHFGNTVYIFTPIYIANYCQNYCIYCGFNCYNNIHRKKLSFEEIEHEMKVI
ADTGMEEILILTGESRKYSDVEYIGESVKIARKYFKNIGIEIYPVNVDEYRYLHECGVDYVTVFQETYNPVKYETLHLLG
HKRVWPYRFDAQERALIAGMRGAGFSALLGLDDFRKDALATALHVYYLNRKYPHAELSLSCPRLRPIINNDKINPRDVGE
KQLCQVLCAYRLFLPFVGITVSSRESAQFRNGITKICATKVSAGVSTGIGDHESKYTGKKDGDAGDEQFEINDNRSFDRM
YKDMEDGGLQPVVNEYIYV

Sequences:

>Translated_419_residues
MNEENQVYFIDSDQLPPAALERKHKLEQDPSFRTDYMKYLDDMEIIDSDIKDKVLKAMDEFDYDSYTAADVERALSKDTC
NIEDFKALLSPAAAPYLEQMARKAENETKKHFGNTVYIFTPIYIANYCQNYCIYCGFNCYNNIHRKKLSFEEIEHEMKVI
ADTGMEEILILTGESRKYSDVEYIGESVKIARKYFKNIGIEIYPVNVDEYRYLHECGVDYVTVFQETYNPVKYETLHLLG
HKRVWPYRFDAQERALIAGMRGAGFSALLGLDDFRKDALATALHVYYLNRKYPHAELSLSCPRLRPIINNDKINPRDVGE
KQLCQVLCAYRLFLPFVGITVSSRESAQFRNGITKICATKVSAGVSTGIGDHESKYTGKKDGDAGDEQFEINDNRSFDRM
YKDMEDGGLQPVVNEYIYV
>Mature_419_residues
MNEENQVYFIDSDQLPPAALERKHKLEQDPSFRTDYMKYLDDMEIIDSDIKDKVLKAMDEFDYDSYTAADVERALSKDTC
NIEDFKALLSPAAAPYLEQMARKAENETKKHFGNTVYIFTPIYIANYCQNYCIYCGFNCYNNIHRKKLSFEEIEHEMKVI
ADTGMEEILILTGESRKYSDVEYIGESVKIARKYFKNIGIEIYPVNVDEYRYLHECGVDYVTVFQETYNPVKYETLHLLG
HKRVWPYRFDAQERALIAGMRGAGFSALLGLDDFRKDALATALHVYYLNRKYPHAELSLSCPRLRPIINNDKINPRDVGE
KQLCQVLCAYRLFLPFVGITVSSRESAQFRNGITKICATKVSAGVSTGIGDHESKYTGKKDGDAGDEQFEINDNRSFDRM
YKDMEDGGLQPVVNEYIYV

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=352, Percent_Identity=37.2159090909091, Blast_Score=238, Evalue=7e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 48326; Mature: 48326

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEENQVYFIDSDQLPPAALERKHKLEQDPSFRTDYMKYLDDMEIIDSDIKDKVLKAMDE
CCCCCEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDYDSYTAADVERALSKDTCNIEDFKALLSPAAAPYLEQMARKAENETKKHFGNTVYIFT
CCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
PIYIANYCQNYCIYCGFNCYNNIHRKKLSFEEIEHEMKVIADTGMEEILILTGESRKYSD
HHHHHHHHHCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCH
VEYIGESVKIARKYFKNIGIEIYPVNVDEYRYLHECGVDYVTVFQETYNPVKYETLHLLG
HHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHC
HKRVWPYRFDAQERALIAGMRGAGFSALLGLDDFRKDALATALHVYYLNRKYPHAELSLS
CCCCCCEECCCHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
CPRLRPIINNDKINPRDVGEKQLCQVLCAYRLFLPFVGITVSSRESAQFRNGITKICATK
CCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHH
VSAGVSTGIGDHESKYTGKKDGDAGDEQFEINDNRSFDRMYKDMEDGGLQPVVNEYIYV
HHCCHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure
MNEENQVYFIDSDQLPPAALERKHKLEQDPSFRTDYMKYLDDMEIIDSDIKDKVLKAMDE
CCCCCEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDYDSYTAADVERALSKDTCNIEDFKALLSPAAAPYLEQMARKAENETKKHFGNTVYIFT
CCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
PIYIANYCQNYCIYCGFNCYNNIHRKKLSFEEIEHEMKVIADTGMEEILILTGESRKYSD
HHHHHHHHHCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCH
VEYIGESVKIARKYFKNIGIEIYPVNVDEYRYLHECGVDYVTVFQETYNPVKYETLHLLG
HHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHC
HKRVWPYRFDAQERALIAGMRGAGFSALLGLDDFRKDALATALHVYYLNRKYPHAELSLS
CCCCCCEECCCHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
CPRLRPIINNDKINPRDVGEKQLCQVLCAYRLFLPFVGITVSSRESAQFRNGITKICATK
CCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHH
VSAGVSTGIGDHESKYTGKKDGDAGDEQFEINDNRSFDRMYKDMEDGGLQPVVNEYIYV
HHCCHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]