Click here to switch to the map view.

The map label for this gene is thiE [H]

Identifier: 238916423

GI number: 238916423

Start: 487873

End: 488499

Strand: Direct

Name: thiE [H]

Synonym: EUBELI_00479

Alternate gene names: 238916423

Gene position: 487873-488499 (Clockwise)

Preceding gene: 238916422

Following gene: 238916424

Centisome position: 22.75

GC content: 42.26

Gene sequence:

>627_bases
ATGAATACATTTATGTATAGAAAATATATATGTATAACGAACAAGCACCTTGTGAGTGGCAGCGGTGGTGCAGAGTGTAC
TATGAATGAGTACATTATGCAGCTTGAAAAATGTGTGGCACTGCACCCATATGCCATGATACTTCGGGAAAAGGATATGG
ACAGGCAGAACTACAGGAAACTTGCAAGACGCATACAGGTAATGTGCAAGAAAGCTTCTGTTAAAATGTTTGTTCACAGT
GATATCAGTGCGGCAGAGTATGCAGGCTGTGGAAATGTACATTTTTCAATGGAACATTTTAAACAAATGTGTGATGAACA
GGCTGATGTGATAAAGGGGCTTGATTGTATGAGTGTATCGTGTCACAGTATGGAAGAGGCAGTGATTGCTGTCCGTGCAG
GAGCAGACCAGATTGTGCTTGGAACTATATTTGAGACGCAGTGTAAACCGGGGAAGATGGGCGCAGGGCTTAGTTTTCTT
GAAGAAGTGTGTAGAGCAGTTCATACTGTTAATCCCACAGTGAAAGTGTTTGCCATTGGAGGAATTAAGCCTGATAATAT
CGAGGAAGTGCTTGAAGCCGGTGCAGATGGCGGATGTATGATGTCATGGTTTATGCAACAAGATTAA

Upstream 100 bases:

>100_bases
GATGGTGATGCCGGAGATGAACAGTTTGAAATCAATGATAACCGTTCATTTGACAGAATGTATAAGGATATGGAGGATGG
CGGGCTTCAGCCAGTCGTGA

Downstream 100 bases:

>100_bases
TATAATAGACAAAGAAAAACTTCTGTGATATAATTTCGCGGAAGTTTTTCTTTTTTAAACTTATTTAAACTTCGTTTCGT
ATAATAATATATAAAACTAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MNTFMYRKYICITNKHLVSGSGGAECTMNEYIMQLEKCVALHPYAMILREKDMDRQNYRKLARRIQVMCKKASVKMFVHS
DISAAEYAGCGNVHFSMEHFKQMCDEQADVIKGLDCMSVSCHSMEEAVIAVRAGADQIVLGTIFETQCKPGKMGAGLSFL
EEVCRAVHTVNPTVKVFAIGGIKPDNIEEVLEAGADGGCMMSWFMQQD

Sequences:

>Translated_208_residues
MNTFMYRKYICITNKHLVSGSGGAECTMNEYIMQLEKCVALHPYAMILREKDMDRQNYRKLARRIQVMCKKASVKMFVHS
DISAAEYAGCGNVHFSMEHFKQMCDEQADVIKGLDCMSVSCHSMEEAVIAVRAGADQIVLGTIFETQCKPGKMGAGLSFL
EEVCRAVHTVNPTVKVFAIGGIKPDNIEEVLEAGADGGCMMSWFMQQD
>Mature_208_residues
MNTFMYRKYICITNKHLVSGSGGAECTMNEYIMQLEKCVALHPYAMILREKDMDRQNYRKLARRIQVMCKKASVKMFVHS
DISAAEYAGCGNVHFSMEHFKQMCDEQADVIKGLDCMSVSCHSMEEAVIAVRAGADQIVLGTIFETQCKPGKMGAGLSFL
EEVCRAVHTVNPTVKVFAIGGIKPDNIEEVLEAGADGGCMMSWFMQQD

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23172; Mature: 23172

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
7.7 %Met     (Translated Protein)
13.0 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
7.7 %Met     (Mature Protein)
13.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTFMYRKYICITNKHLVSGSGGAECTMNEYIMQLEKCVALHPYAMILREKDMDRQNYRK
CCCCCEEEEEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHH
LARRIQVMCKKASVKMFVHSDISAAEYAGCGNVHFSMEHFKQMCDEQADVIKGLDCMSVS
HHHHHHHHHHHHHEEEEEECCCCHHHHCCCCCEEECHHHHHHHHHHHHHHHHCCHHHHHH
CHSMEEAVIAVRAGADQIVLGTIFETQCKPGKMGAGLSFLEEVCRAVHTVNPTVKVFAIG
HHHHHHHHEEEECCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEC
GIKPDNIEEVLEAGADGGCMMSWFMQQD
CCCCCCHHHHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MNTFMYRKYICITNKHLVSGSGGAECTMNEYIMQLEKCVALHPYAMILREKDMDRQNYRK
CCCCCEEEEEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHH
LARRIQVMCKKASVKMFVHSDISAAEYAGCGNVHFSMEHFKQMCDEQADVIKGLDCMSVS
HHHHHHHHHHHHHEEEEEECCCCHHHHCCCCCEEECHHHHHHHHHHHHHHHHCCHHHHHH
CHSMEEAVIAVRAGADQIVLGTIFETQCKPGKMGAGLSFLEEVCRAVHTVNPTVKVFAIG
HHHHHHHHEEEECCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEC
GIKPDNIEEVLEAGADGGCMMSWFMQQD
CCCCCCHHHHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA