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The map label for this gene is pehX [H]

Identifier: 238916395

GI number: 238916395

Start: 459675

End: 461051

Strand: Direct

Name: pehX [H]

Synonym: EUBELI_00449

Alternate gene names: 238916395

Gene position: 459675-461051 (Clockwise)

Preceding gene: 238916394

Following gene: 238916397

Centisome position: 21.44

GC content: 37.69

Gene sequence:

>1377_bases
ATGAAAAAAGTTAATTTTGATGTAAAACTCCCAGCATTTGTGGAGAGAATAGTATATGTTGATAACTATGGTGCAAGACC
ATACTGTTACACTATTGAGGATGCATCAAGAAATGCGGATGCGATAAACAGGGCTATTAACTATATTTCAGAGAAGGGTG
GCGGAACTGTTGTGATTCCTGATGGAATCTGGTTTACAGCACCAATTGAGATTAAGAGTGATGTTGAGTTAAGAATTGAG
AAGAATGCAATACTTAAATTTTCTAAAGATATTGACCAGTATCCGCTTATTATCACTAACTATGAAGGTCAGGAATGTAT
AAGAGCCAAGTCACCTATTACAGCTGAAAATGCTATTAACATCGGTATAACAGGTGGCGGTGTTATTGATGGAAGTGGTG
ATATGTGGCGCCCTATAAAACAGTTTAAGATAACTGACAGACAGTGGGAAGCTCTTATGAAAAAGAGCCAGTATATAATT
GATACTAAAGGGGGCGGCATATGGATGCCGACAGAAAGCTCATTTAAAGGTAATGAGCATAATATCCAGCTTGACGCTGA
GAATGCACTTGAGAAAGCAAGCGAATATTATGATTTCTACAGACCTGTAATGGTAAGCCTTCGTCACTGTAAAAGAATTC
TTCTTGATGGTGTAACATTTAAGAATTCACCAGCATGGAATATACACCCATTTTTCTGTAAGAATCTTACAGTAAGAAAT
GTTACGGTGAGCAATCCTTATTATGCGCAGAACGGTGATGGAATTGATGTTGAATCATGCAAGAAGGTTCATATACACAA
CTGTACATTTGAAACAGGTGATGATGCAATCTGCTTAAAGTCAGGCAAGAATGCCGTCGCAAGACAGATTGAGGGACCTT
GTGAGGATGTATATATCCATGATTGTCTCGTTAATGAGGGACATGGCGGCTTCGTTATCGGAAGCGAGATGAGCCGTGGT
ATTAAAAATGTATTGGTAGAGAATTGTACTTTCCTTGGAACTGATGTCGGAGTCCGCATTAAGAGCGCACTTGGACGAGG
TGGCGTTATCGAAAATATTAACATAAAGAATATTAATATGGTTGATATTAAGGAACAGGCAATTATTCTTACAATGTCAT
ATGTGCTTAATTCTCTTAACAGAGATGAAGAAATTAACGGAATCGACAAAGACGACATTCCTTATTTTAAGAATATTAAT
TTTGAAGGAATTAACTGTCTTGGAGCAAAGGAAGCGGTTGTTATTGAACCGATAAAGGATATGCCTGAGACAATCACAGA
TATACACATTAAGGCTTCATCATTTGTTACAAGTGGTGAGAACCGTGTTGGATGTGACTGCCTTACAAGTGAACAGACAA
CTTATGAGATATTATAA

Upstream 100 bases:

>100_bases
TGAAGGCAATGCAGGGAAAAGCTAATCCGGGAATGGTTAATGCTCTTTTACAGGAGCTTTTAAAGTAACACATGAACTTA
AAACTAGAAGGAGATGTGGA

Downstream 100 bases:

>100_bases
AGAAATCCCGGCAGCACTGATATATGTGCTGCTGGGATTTATGCGTTGTGTCTTAATTTTTCTTTTCAATCTTAGCCTCA
TATTCATCAAAGATATAACC

Product: glycoside hydrolase family 28-like polygalacturonase

Products: NA

Alternate protein names: Exo-PG [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKKVNFDVKLPAFVERIVYVDNYGARPYCYTIEDASRNADAINRAINYISEKGGGTVVIPDGIWFTAPIEIKSDVELRIE
KNAILKFSKDIDQYPLIITNYEGQECIRAKSPITAENAINIGITGGGVIDGSGDMWRPIKQFKITDRQWEALMKKSQYII
DTKGGGIWMPTESSFKGNEHNIQLDAENALEKASEYYDFYRPVMVSLRHCKRILLDGVTFKNSPAWNIHPFFCKNLTVRN
VTVSNPYYAQNGDGIDVESCKKVHIHNCTFETGDDAICLKSGKNAVARQIEGPCEDVYIHDCLVNEGHGGFVIGSEMSRG
IKNVLVENCTFLGTDVGVRIKSALGRGGVIENINIKNINMVDIKEQAIILTMSYVLNSLNRDEEINGIDKDDIPYFKNIN
FEGINCLGAKEAVVIEPIKDMPETITDIHIKASSFVTSGENRVGCDCLTSEQTTYEIL

Sequences:

>Translated_458_residues
MKKVNFDVKLPAFVERIVYVDNYGARPYCYTIEDASRNADAINRAINYISEKGGGTVVIPDGIWFTAPIEIKSDVELRIE
KNAILKFSKDIDQYPLIITNYEGQECIRAKSPITAENAINIGITGGGVIDGSGDMWRPIKQFKITDRQWEALMKKSQYII
DTKGGGIWMPTESSFKGNEHNIQLDAENALEKASEYYDFYRPVMVSLRHCKRILLDGVTFKNSPAWNIHPFFCKNLTVRN
VTVSNPYYAQNGDGIDVESCKKVHIHNCTFETGDDAICLKSGKNAVARQIEGPCEDVYIHDCLVNEGHGGFVIGSEMSRG
IKNVLVENCTFLGTDVGVRIKSALGRGGVIENINIKNINMVDIKEQAIILTMSYVLNSLNRDEEINGIDKDDIPYFKNIN
FEGINCLGAKEAVVIEPIKDMPETITDIHIKASSFVTSGENRVGCDCLTSEQTTYEIL
>Mature_458_residues
MKKVNFDVKLPAFVERIVYVDNYGARPYCYTIEDASRNADAINRAINYISEKGGGTVVIPDGIWFTAPIEIKSDVELRIE
KNAILKFSKDIDQYPLIITNYEGQECIRAKSPITAENAINIGITGGGVIDGSGDMWRPIKQFKITDRQWEALMKKSQYII
DTKGGGIWMPTESSFKGNEHNIQLDAENALEKASEYYDFYRPVMVSLRHCKRILLDGVTFKNSPAWNIHPFFCKNLTVRN
VTVSNPYYAQNGDGIDVESCKKVHIHNCTFETGDDAICLKSGKNAVARQIEGPCEDVYIHDCLVNEGHGGFVIGSEMSRG
IKNVLVENCTFLGTDVGVRIKSALGRGGVIENINIKNINMVDIKEQAIILTMSYVLNSLNRDEEINGIDKDDIPYFKNIN
FEGINCLGAKEAVVIEPIKDMPETITDIHIKASSFVTSGENRVGCDCLTSEQTTYEIL

Specific function: Contributes significantly to bacterial utilization of polygalacturonate and the induction of pectate lyase in the presence of extracellular pectic polymers [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR000743
- InterPro:   IPR013783
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00041 fn3; PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.82 [H]

Molecular weight: Translated: 51114; Mature: 51114

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00502 POLYGALACTURONASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVNFDVKLPAFVERIVYVDNYGARPYCYTIEDASRNADAINRAINYISEKGGGTVVIP
CCCEEEEEECHHHHEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEE
DGIWFTAPIEIKSDVELRIEKNAILKFSKDIDQYPLIITNYEGQECIRAKSPITAENAIN
CCEEEEEEEEECCCEEEEEECCEEEEECCCCCCCCEEEECCCCHHHHHCCCCCCCCCEEE
IGITGGGVIDGSGDMWRPIKQFKITDRQWEALMKKSQYIIDTKGGGIWMPTESSFKGNEH
EEEECCEEECCCCCCCCHHHHHCCCHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCCCC
NIQLDAENALEKASEYYDFYRPVMVSLRHCKRILLDGVTFKNSPAWNIHPFFCKNLTVRN
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHCCCEEEEE
VTVSNPYYAQNGDGIDVESCKKVHIHNCTFETGDDAICLKSGKNAVARQIEGPCEDVYIH
EEECCCEEECCCCCCCHHHCCEEEEEECEEECCCCEEEEECCCHHHHHHCCCCCCEEEEE
DCLVNEGHGGFVIGSEMSRGIKNVLVENCTFLGTDVGVRIKSALGRGGVIENINIKNINM
EEEEECCCCCEEECHHHHHHHHHHHHHCCEEEECCCCEEEHHHCCCCCEEEECCCCCCEE
VDIKEQAIILTMSYVLNSLNRDEEINGIDKDDIPYFKNINFEGINCLGAKEAVVIEPIKD
EEECCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCEEECCCCCEEEECCHHC
MPETITDIHIKASSFVTSGENRVGCDCLTSEQTTYEIL
CCHHHHEEEEEEHHEEECCCCCCCCEEECCCCCEEEEC
>Mature Secondary Structure
MKKVNFDVKLPAFVERIVYVDNYGARPYCYTIEDASRNADAINRAINYISEKGGGTVVIP
CCCEEEEEECHHHHEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEE
DGIWFTAPIEIKSDVELRIEKNAILKFSKDIDQYPLIITNYEGQECIRAKSPITAENAIN
CCEEEEEEEEECCCEEEEEECCEEEEECCCCCCCCEEEECCCCHHHHHCCCCCCCCCEEE
IGITGGGVIDGSGDMWRPIKQFKITDRQWEALMKKSQYIIDTKGGGIWMPTESSFKGNEH
EEEECCEEECCCCCCCCHHHHHCCCHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCCCC
NIQLDAENALEKASEYYDFYRPVMVSLRHCKRILLDGVTFKNSPAWNIHPFFCKNLTVRN
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHCCCEEEEE
VTVSNPYYAQNGDGIDVESCKKVHIHNCTFETGDDAICLKSGKNAVARQIEGPCEDVYIH
EEECCCEEECCCCCCCHHHCCEEEEEECEEECCCCEEEEECCCHHHHHHCCCCCCEEEEE
DCLVNEGHGGFVIGSEMSRGIKNVLVENCTFLGTDVGVRIKSALGRGGVIENINIKNINM
EEEEECCCCCEEECHHHHHHHHHHHHHCCEEEECCCCEEEHHHCCCCCEEEECCCCCCEE
VDIKEQAIILTMSYVLNSLNRDEEINGIDKDDIPYFKNINFEGINCLGAKEAVVIEPIKD
EEECCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCEEECCCCCEEEECCHHC
MPETITDIHIKASSFVTSGENRVGCDCLTSEQTTYEIL
CCHHHHEEEEEEHHEEECCCCCCCCEEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2168372; 1409594 [H]