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The map label for this gene is gph [H]

Identifier: 238916386

GI number: 238916386

Start: 451025

End: 451675

Strand: Direct

Name: gph [H]

Synonym: EUBELI_00440

Alternate gene names: 238916386

Gene position: 451025-451675 (Clockwise)

Preceding gene: 238916385

Following gene: 238916388

Centisome position: 21.03

GC content: 39.63

Gene sequence:

>651_bases
ATGAAATACAAAATTGTTGTATTTGATCTGGACGGAACGATACTTAATACATTGGAAGACCTTGCAGATAGTACTAATTA
TGCACTTTCAGTATTTGACATGCCTGAGAGGACGATTGAAGAAATCAGACATTTTGTTGGTAATGGGATAAGAAAGCTGA
TGGAAAGAGCAGTACCACAGGGAACACCAGAAAGCATGATAGTTAAGGTGCATGAAACATTTACAGAGCATTACAAGAAG
CACTGTGCCGATAAGACAAGACCTTATGATGGAATAAAAGAACTTATTGAGAATATAAGAAATGCCGGTGTGTACACTGC
GGTTGTATCTAACAAGGCTGATTATGGAGTGCAGATTTTATGCAAAGATTATTTTCCGGGACTATTTGATTATGCGGTTG
GAGAGAGAGAAGGCATAAGAAGAAAACCATATCCGGACAGCGTTAATGAAGTATTAAGACAGTTTGATGTTGACAGAACA
CAGGCTGTATATGTCGGGGATTCAGAAGTAGATGTCCAGACAGCGGCTAATGCAGGAATGGATGTATGTATGGTTGGCTG
GGGCTTCAGAGATGAAGAATTCTTAAAGGAAAACGGGGCTGAGTTTGTTGTACACAGCCCCGAAGAAGCATGGGAATTCA
TTAATAAGTAA

Upstream 100 bases:

>100_bases
TTATAAAGAAGGATATTGAGACACTTAATGCAGCGATTGATGAGCATTACGATATAATTGAGGAGCTGCTGGGATAAAGA
AGTCATAACAAGGAGGATAT

Downstream 100 bases:

>100_bases
TATACTTATACCACGTTATGAAGCAGGGAAGACTGCAATTCACAAAGCTGGGGGTTCTTGGATTCACGAATCAGTTCTAC
CAGACCTTCGGTGATAAGCA

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKYKIVVFDLDGTILNTLEDLADSTNYALSVFDMPERTIEEIRHFVGNGIRKLMERAVPQGTPESMIVKVHETFTEHYKK
HCADKTRPYDGIKELIENIRNAGVYTAVVSNKADYGVQILCKDYFPGLFDYAVGEREGIRRKPYPDSVNEVLRQFDVDRT
QAVYVGDSEVDVQTAANAGMDVCMVGWGFRDEEFLKENGAEFVVHSPEEAWEFINK

Sequences:

>Translated_216_residues
MKYKIVVFDLDGTILNTLEDLADSTNYALSVFDMPERTIEEIRHFVGNGIRKLMERAVPQGTPESMIVKVHETFTEHYKK
HCADKTRPYDGIKELIENIRNAGVYTAVVSNKADYGVQILCKDYFPGLFDYAVGEREGIRRKPYPDSVNEVLRQFDVDRT
QAVYVGDSEVDVQTAANAGMDVCMVGWGFRDEEFLKENGAEFVVHSPEEAWEFINK
>Mature_216_residues
MKYKIVVFDLDGTILNTLEDLADSTNYALSVFDMPERTIEEIRHFVGNGIRKLMERAVPQGTPESMIVKVHETFTEHYKK
HCADKTRPYDGIKELIENIRNAGVYTAVVSNKADYGVQILCKDYFPGLFDYAVGEREGIRRKPYPDSVNEVLRQFDVDRT
QAVYVGDSEVDVQTAANAGMDVCMVGWGFRDEEFLKENGAEFVVHSPEEAWEFINK

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=205, Percent_Identity=24.8780487804878, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24646; Mature: 24646

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKIVVFDLDGTILNTLEDLADSTNYALSVFDMPERTIEEIRHFVGNGIRKLMERAVPQ
CCEEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
GTPESMIVKVHETFTEHYKKHCADKTRPYDGIKELIENIRNAGVYTAVVSNKADYGVQIL
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEE
CKDYFPGLFDYAVGEREGIRRKPYPDSVNEVLRQFDVDRTQAVYVGDSEVDVQTAANAGM
EHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCCC
DVCMVGWGFRDEEFLKENGAEFVVHSPEEAWEFINK
CEEEECCCCCCHHHHHCCCCEEEEECHHHHHHHHCC
>Mature Secondary Structure
MKYKIVVFDLDGTILNTLEDLADSTNYALSVFDMPERTIEEIRHFVGNGIRKLMERAVPQ
CCEEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
GTPESMIVKVHETFTEHYKKHCADKTRPYDGIKELIENIRNAGVYTAVVSNKADYGVQIL
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEE
CKDYFPGLFDYAVGEREGIRRKPYPDSVNEVLRQFDVDRTQAVYVGDSEVDVQTAANAGM
EHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCCC
DVCMVGWGFRDEEFLKENGAEFVVHSPEEAWEFINK
CEEEECCCCCCHHHHHCCCCEEEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]