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The map label for this gene is yclM [H]

Identifier: 238916337

GI number: 238916337

Start: 402035

End: 403351

Strand: Direct

Name: yclM [H]

Synonym: EUBELI_00371

Alternate gene names: 238916337

Gene position: 402035-403351 (Clockwise)

Preceding gene: 238916336

Following gene: 238916338

Centisome position: 18.75

GC content: 38.34

Gene sequence:

>1317_bases
ATGGTTAAAGTAGTTAAATTTGGTGGAAGTTCACTTGCAAGCGCAGAGCAGTTTAAGAAGGTTGGTAATATTATCCGTGC
TGATGAGGACAGAAAGTATGTAGTTCCTTCAGCACCTGGAAAGAGATTTCCAGAGGATACTAAGGTTACTGATATGCTTT
ACAAATGTTATGCAGAGGCAGAAGCTGGTAAGAATATTGAGAAGAGCCTTAAGGCAATTGAGGAAAGATATAACGAAATA
ATAAAGGGGCTTGGACTTAAGCTTTCATTAAAGGAACAGTTCGAGACAATCAAGAAGAATTTTGAGGCACTTGCAGGAAA
AGATTATGCAGCTTCAAGAGGTGAATATCTTAATGGTATTATCATGGCTAACTATCTTGGATATGAGTTCATAGACGCTG
CTACAGTTATCTGCTTCGATAAGGATGGAGAGTTTGATTCAGAAAAGACTAATGAAGTGTGTGAAGCAAAGTTTGCTAAT
ATTGTAAGAGCAGTTGTACCTGGATTTTATGGAGCAATGCCTAACGGAAAGGTTAAGACATTTTCAAGAGGCGGTTCTGA
TGTAACAGGATCAATCGTTGCAAGAGCACTTAAGGCTGATATGTATGAGAACTGGACAGATGTATCAGGCTTCCTTGCAG
CAGATCCAAGAATTGTAAATAATGCAAAGCCAATCAACGTTATTACATATAAGGAATTAAGAGAGCTTTCATACATGGGT
GCATCAGTGCTTCATGAATCAGCTATCTTCCCTGTAAGAAAGGGTGGAATTCCTATCAATATTAAGAATACTAATGCTCC
GGAAGATAAGGGAACAATGATTGTTGAGACAACATGTAATATGCCGGAATACACAATTACTGGTATTGCAGGTAAGAAGG
GATTCGTTGCAATCAGCATTGATAAGGATATGATGAATTCAGAAATAGGATTCTGTAGAAAGGTTCTTTCAGTATTCGAG
GATAACGGAATCTCAATCGAACATATGCCTTCAGGAATTGATACTATGACAGTATTTGTTCATCAGGATGAATTCGTAGA
GAAGGAACAGAAGGTCCTTGCGCAGATTCATAAGGCAGTTAATCCGGATTCTGTTGAGCTTGAAGCTAACCTTGCACTTA
TTGCGGTAGTCGGACGTGGAATGAAGAACAGTACAGGTATTGCTGGTAATATCTTCTCAGCACTTGCAAAAGCCAAGATT
AATGTTAAAATGATAGATCAGGGATCTTCAGAGCTTAACATCATCATTGGCGTAAGAAACAGATACTTTGAGGATGCTAT
TAAGACTATCTATGGAGTGTTTGTTCCAGAAGAATAA

Upstream 100 bases:

>100_bases
AGAGAGAGCATTTGAAGATGCTAAGGATAAGGTTTCTGTTATAAGCAGAATCAGAATAGATGATACTAAATTACAGTAAT
AATATAAGGGAGTAAGAGTA

Downstream 100 bases:

>100_bases
TAAGTGAATAGGGGGATATGGGATATGAAGGAAGCTAAGAATGAGTTGTTACAGTTTGTCGGCGGCGTAATAATGCTTGT
AGTAGGACTGTACATATTGT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MVKVVKFGGSSLASAEQFKKVGNIIRADEDRKYVVPSAPGKRFPEDTKVTDMLYKCYAEAEAGKNIEKSLKAIEERYNEI
IKGLGLKLSLKEQFETIKKNFEALAGKDYAASRGEYLNGIIMANYLGYEFIDAATVICFDKDGEFDSEKTNEVCEAKFAN
IVRAVVPGFYGAMPNGKVKTFSRGGSDVTGSIVARALKADMYENWTDVSGFLAADPRIVNNAKPINVITYKELRELSYMG
ASVLHESAIFPVRKGGIPINIKNTNAPEDKGTMIVETTCNMPEYTITGIAGKKGFVAISIDKDMMNSEIGFCRKVLSVFE
DNGISIEHMPSGIDTMTVFVHQDEFVEKEQKVLAQIHKAVNPDSVELEANLALIAVVGRGMKNSTGIAGNIFSALAKAKI
NVKMIDQGSSELNIIIGVRNRYFEDAIKTIYGVFVPEE

Sequences:

>Translated_438_residues
MVKVVKFGGSSLASAEQFKKVGNIIRADEDRKYVVPSAPGKRFPEDTKVTDMLYKCYAEAEAGKNIEKSLKAIEERYNEI
IKGLGLKLSLKEQFETIKKNFEALAGKDYAASRGEYLNGIIMANYLGYEFIDAATVICFDKDGEFDSEKTNEVCEAKFAN
IVRAVVPGFYGAMPNGKVKTFSRGGSDVTGSIVARALKADMYENWTDVSGFLAADPRIVNNAKPINVITYKELRELSYMG
ASVLHESAIFPVRKGGIPINIKNTNAPEDKGTMIVETTCNMPEYTITGIAGKKGFVAISIDKDMMNSEIGFCRKVLSVFE
DNGISIEHMPSGIDTMTVFVHQDEFVEKEQKVLAQIHKAVNPDSVELEANLALIAVVGRGMKNSTGIAGNIFSALAKAKI
NVKMIDQGSSELNIIIGVRNRYFEDAIKTIYGVFVPEE
>Mature_438_residues
MVKVVKFGGSSLASAEQFKKVGNIIRADEDRKYVVPSAPGKRFPEDTKVTDMLYKCYAEAEAGKNIEKSLKAIEERYNEI
IKGLGLKLSLKEQFETIKKNFEALAGKDYAASRGEYLNGIIMANYLGYEFIDAATVICFDKDGEFDSEKTNEVCEAKFAN
IVRAVVPGFYGAMPNGKVKTFSRGGSDVTGSIVARALKADMYENWTDVSGFLAADPRIVNNAKPINVITYKELRELSYMG
ASVLHESAIFPVRKGGIPINIKNTNAPEDKGTMIVETTCNMPEYTITGIAGKKGFVAISIDKDMMNSEIGFCRKVLSVFE
DNGISIEHMPSGIDTMTVFVHQDEFVEKEQKVLAQIHKAVNPDSVELEANLALIAVVGRGMKNSTGIAGNIFSALAKAKI
NVKMIDQGSSELNIIIGVRNRYFEDAIKTIYGVFVPEE

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=453, Percent_Identity=28.476821192053, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1790455, Length=453, Percent_Identity=24.7240618101545, Blast_Score=125, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320893, Length=459, Percent_Identity=25.9259259259259, Blast_Score=120, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 48098; Mature: 48098

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKVVKFGGSSLASAEQFKKVGNIIRADEDRKYVVPSAPGKRFPEDTKVTDMLYKCYAEA
CEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHH
EAGKNIEKSLKAIEERYNEIIKGLGLKLSLKEQFETIKKNFEALAGKDYAASRGEYLNGI
HCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCE
IMANYLGYEFIDAATVICFDKDGEFDSEKTNEVCEAKFANIVRAVVPGFYGAMPNGKVKT
EEEHHCCHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
FSRGGSDVTGSIVARALKADMYENWTDVSGFLAADPRIVNNAKPINVITYKELRELSYMG
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCEEECCCCCEEEEEHHHHHHHHHHH
ASVLHESAIFPVRKGGIPINIKNTNAPEDKGTMIVETTCNMPEYTITGIAGKKGFVAISI
HHHHHHHCCCEEECCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEE
DKDMMNSEIGFCRKVLSVFEDNGISIEHMPSGIDTMTVFVHQDEFVEKEQKVLAQIHKAV
CHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECHHHHHHHHHHHHHHHHHC
NPDSVELEANLALIAVVGRGMKNSTGIAGNIFSALAKAKINVKMIDQGSSELNIIIGVRN
CCCCEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCEEEEEEECH
RYFEDAIKTIYGVFVPEE
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVKVVKFGGSSLASAEQFKKVGNIIRADEDRKYVVPSAPGKRFPEDTKVTDMLYKCYAEA
CEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHH
EAGKNIEKSLKAIEERYNEIIKGLGLKLSLKEQFETIKKNFEALAGKDYAASRGEYLNGI
HCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCE
IMANYLGYEFIDAATVICFDKDGEFDSEKTNEVCEAKFANIVRAVVPGFYGAMPNGKVKT
EEEHHCCHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
FSRGGSDVTGSIVARALKADMYENWTDVSGFLAADPRIVNNAKPINVITYKELRELSYMG
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCEEECCCCCEEEEEHHHHHHHHHHH
ASVLHESAIFPVRKGGIPINIKNTNAPEDKGTMIVETTCNMPEYTITGIAGKKGFVAISI
HHHHHHHCCCEEECCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEE
DKDMMNSEIGFCRKVLSVFEDNGISIEHMPSGIDTMTVFVHQDEFVEKEQKVLAQIHKAV
CHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECHHHHHHHHHHHHHHHHHC
NPDSVELEANLALIAVVGRGMKNSTGIAGNIFSALAKAKINVKMIDQGSSELNIIIGVRN
CCCCEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCEEEEEEECH
RYFEDAIKTIYGVFVPEE
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]