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The map label for this gene is dusC [H]

Identifier: 238916319

GI number: 238916319

Start: 380030

End: 381031

Strand: Direct

Name: dusC [H]

Synonym: EUBELI_00353

Alternate gene names: 238916319

Gene position: 380030-381031 (Clockwise)

Preceding gene: 238916318

Following gene: 238916321

Centisome position: 17.72

GC content: 39.92

Gene sequence:

>1002_bases
ATGGCCCCAATGGAGGGGCTTACAGATTTTACATACCGTAACGCCTACGAGAAAACATTTGGAAAAGGCAGAATTACAAA
ATATTTTACACCGTTTATCTCACCTAATAAGTCAGAGAATTTTCTTGCAAGAGAGATAAGGGACATAGACCGCGGGCATA
ATAAGGATACATATACGGTTGTACAGGTCATGACTAATGAAGCTAAGGATTTTATATGGACAGCAAAAATGCTTTATGAA
AAATACGGCTACAGCGAGATTAATCTTAATGCCGGCTGTCCGTCAGGCACAGTTGTTTCAAAGGATAAAGGCTCAGGAAT
GCTTCGCGACACAGAAAAGCTCGACCGTTTTCTTGATGAGGTGTTTGAGGATGCATTTATAAGAGAGAATATTAAGGTTT
CGGTCAAGACACGAATCGGTGTTGAAGATGATGACAGTTTTCCACAGATAATGGAGGTGTATAATTCTTATCCTCTTGAG
GAACTTATTGTTCATCCGAGAGTGCGTACAGATTATTACAGAAATGAGCTGCATCTTGACGCTTTCCGTTATGCTGTTGA
TAACAGCAGGAATAAATTAGTGCTTAACGGAGATATATTTACAAGGAAGAATTTCCTTGAAATGAAGGAGATGTTTCCGG
AGGTTGATACATTTATGCTTGGAAGAGGGCTTATCGCGGACCCCGGACTGATTAATGTATTAACAGATGATAATCCTCAG
GCGATGGTGCGGGACCTTAATGCAGACAAGAAGCTGATGAAAGAGCTTCACGACCTTGTATACGCAGCCCGCACAGCCAT
AATGCCGGGAGATACACATGCCATACACAGAATGAAAGAAATGTGGTGTTATATGGAATATGTGTTTGATGACTGCAAAA
AAGAAATCAAGGCAATCAAGAAATCCCAGAGAATGGCTGATTACAAGGCTGCAGTTGATGTGTTCTTCAACAAGGCAATG
CTTGTTGAAAGAAAGAATATTATATTTTCAAAAAAATTCTAA

Upstream 100 bases:

>100_bases
GCAGTAAAAGAATTTTTCGAGACAGCCATTGTGACTAACAGCAACGGCACATTTACATTAGAATAACAGAAAGATACAGT
ATATGAGCAATACTATATAT

Downstream 100 bases:

>100_bases
ATCAGTGACAGGCAGTATCATATCAGATATTGTCTGTCTTCTTTTTCTGCTGGTGACCTGTCATAAAGGCAAGCACCTGT
CTTGGCAGGAATCTGCTGCC

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MAPMEGLTDFTYRNAYEKTFGKGRITKYFTPFISPNKSENFLAREIRDIDRGHNKDTYTVVQVMTNEAKDFIWTAKMLYE
KYGYSEINLNAGCPSGTVVSKDKGSGMLRDTEKLDRFLDEVFEDAFIRENIKVSVKTRIGVEDDDSFPQIMEVYNSYPLE
ELIVHPRVRTDYYRNELHLDAFRYAVDNSRNKLVLNGDIFTRKNFLEMKEMFPEVDTFMLGRGLIADPGLINVLTDDNPQ
AMVRDLNADKKLMKELHDLVYAARTAIMPGDTHAIHRMKEMWCYMEYVFDDCKKEIKAIKKSQRMADYKAAVDVFFNKAM
LVERKNIIFSKKF

Sequences:

>Translated_333_residues
MAPMEGLTDFTYRNAYEKTFGKGRITKYFTPFISPNKSENFLAREIRDIDRGHNKDTYTVVQVMTNEAKDFIWTAKMLYE
KYGYSEINLNAGCPSGTVVSKDKGSGMLRDTEKLDRFLDEVFEDAFIRENIKVSVKTRIGVEDDDSFPQIMEVYNSYPLE
ELIVHPRVRTDYYRNELHLDAFRYAVDNSRNKLVLNGDIFTRKNFLEMKEMFPEVDTFMLGRGLIADPGLINVLTDDNPQ
AMVRDLNADKKLMKELHDLVYAARTAIMPGDTHAIHRMKEMWCYMEYVFDDCKKEIKAIKKSQRMADYKAAVDVFFNKAM
LVERKNIIFSKKF
>Mature_332_residues
APMEGLTDFTYRNAYEKTFGKGRITKYFTPFISPNKSENFLAREIRDIDRGHNKDTYTVVQVMTNEAKDFIWTAKMLYEK
YGYSEINLNAGCPSGTVVSKDKGSGMLRDTEKLDRFLDEVFEDAFIRENIKVSVKTRIGVEDDDSFPQIMEVYNSYPLEE
LIVHPRVRTDYYRNELHLDAFRYAVDNSRNKLVLNGDIFTRKNFLEMKEMFPEVDTFMLGRGLIADPGLINVLTDDNPQA
MVRDLNADKKLMKELHDLVYAARTAIMPGDTHAIHRMKEMWCYMEYVFDDCKKEIKAIKKSQRMADYKAAVDVFFNKAML
VERKNIIFSKKF

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788462, Length=330, Percent_Identity=23.9393939393939, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38878; Mature: 38747

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPMEGLTDFTYRNAYEKTFGKGRITKYFTPFISPNKSENFLAREIRDIDRGHNKDTYTV
CCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHH
VQVMTNEAKDFIWTAKMLYEKYGYSEINLNAGCPSGTVVSKDKGSGMLRDTEKLDRFLDE
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHH
VFEDAFIRENIKVSVKTRIGVEDDDSFPQIMEVYNSYPLEELIVHPRVRTDYYRNELHLD
HHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHH
AFRYAVDNSRNKLVLNGDIFTRKNFLEMKEMFPEVDTFMLGRGLIADPGLINVLTDDNPQ
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEEECCCHH
AMVRDLNADKKLMKELHDLVYAARTAIMPGDTHAIHRMKEMWCYMEYVFDDCKKEIKAIK
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSQRMADYKAAVDVFFNKAMLVERKNIIFSKKF
HHHHHHHHHHHHHHHHHHHHHEECCCCEEECCC
>Mature Secondary Structure 
APMEGLTDFTYRNAYEKTFGKGRITKYFTPFISPNKSENFLAREIRDIDRGHNKDTYTV
CCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHH
VQVMTNEAKDFIWTAKMLYEKYGYSEINLNAGCPSGTVVSKDKGSGMLRDTEKLDRFLDE
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHH
VFEDAFIRENIKVSVKTRIGVEDDDSFPQIMEVYNSYPLEELIVHPRVRTDYYRNELHLD
HHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHH
AFRYAVDNSRNKLVLNGDIFTRKNFLEMKEMFPEVDTFMLGRGLIADPGLINVLTDDNPQ
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEEECCCHH
AMVRDLNADKKLMKELHDLVYAARTAIMPGDTHAIHRMKEMWCYMEYVFDDCKKEIKAIK
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSQRMADYKAAVDVFFNKAMLVERKNIIFSKKF
HHHHHHHHHHHHHHHHHHHHHEECCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]